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Citations for New England Biolabs :
1951 - 2000 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 25 ng of linearised plasmids were ligated with 75 ng of purified VH and VL by Gibson Assembly (New England BioLabs). The reaction was performed in a final volume of 5 μL ...
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bioRxiv - Systems Biology 2024Quote: Approximately 6ng of each loxcode cassette was combined with approximately 75ng of the linear digested plasmid pool in a final 10uL of 1x HiFi assembly reaction (NEB). This was incubated on a thermocycler at 50C for 15min ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR fragment was gel purified and inserted into plasmids pTRC99a and pKI_IS10 (gift of S. Wenk) using HiFi DNA Assembly Cloning Kit (NEB. pZE21) for Gibson cloning ...
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bioRxiv - Biophysics 2023Quote: ... with the restriction enzymes AvrII and HindIII and cloned into the digested plasmid pGJJ191 (described in 24) using T4 ligase (New England Biolabs). AbundancePCA (aPCA ...
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bioRxiv - Genetics 2023Quote: ... Homology arms were obtained by PCR from HEK-293T genomic DNA and a plasmid template was generated per candidate GSH site by Gibson assembly (New England Biolabs) using fragments synthesized by Q5 High Fidelity Taq polymerase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2023Quote: ... The fragments were cloned into a pCS2+ plasmid linearized with Xho1 and Xba1 restriction enzymes using a Quick Ligation Kit (NEB). Each plasmid was then linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription (using the mMESSAGE mMACHINE sp6 Ultra kit AM1340 ...
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bioRxiv - Genomics 2023Quote: ... The backbones for the Gibson reaction for GRB2-SH3 and PSD95-PDZ3 library assembly (aPCA plasmids) were first linearized using primers listed in Extended Data Table 3 and next treated with Dpn1 (NEB) restriction enzyme to remove the circular plasmid template ...
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bioRxiv - Developmental Biology 2023Quote: ... The Sox2(600bp)::GFP plasmid was generated by digesting the primer overhangs of the PCR product with XhoI and EcoRV (NEB) and its consequent ligation into linearised plasmid ISceI/MCS-d1GFP-SV40/ISceI ...
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bioRxiv - Systems Biology 2024Quote: ... We digested ∼2ug of each of 24 barcoded ORF plasmid pools (2 replicate pools of each of 9 hORFs and 3 vORFs) overnight with I-SceI (NEB) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from a pENTR cyfip2-EGFP plasmid [32] using custom primers and the Q5 Site Directed Mutagenesis Kit (NEB) to induce the desired C179R (ΔRac1 ...
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bioRxiv - Neuroscience 2024Quote: ... DIG- and FITC-labeled antisense RNA probes were generated from 1 µg of linearized plasmid template using T7 or Sp6 RNA polymerases (NEB) and DIG-11-UTP (Roche) ...
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bioRxiv - Pathology 2024Quote: ... Prior to transformation the bait plasmids were linearised in the URA3 gene by PCR or restriction digest (BbsI/Esp3I/BstBI (NEB)) to allow integration into the genome ...
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bioRxiv - Neuroscience 2024Quote: ... The codon-optimized APEX2-V5 sequence was synthesized as a gBlock (Integrated DNA Technologies) and inserted into the TOPO genomic sequence plasmid using the NEBuilder HiFi DNA assembly master mix (New England Biolabs). CRISPR guide RNA (gRNA ...
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bioRxiv - Neuroscience 2024Quote: ... a V5 tag was inserted after the start codon of Ten-m cDNA (isoform B) in the plasmid pUAST-attB-Ten-m5 using the Q5 site-directed mutagenesis kit (New England Biolabs). To generate the UAS-V5-Ten-m-ΔICD and UAS-V5-Ten-m-ΔECD constructs ...
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bioRxiv - Microbiology 2024Quote: ... the plasmid pME6000 was digested by HindIII and the PCR-amplified gene of interest was inserted by Gibson cloning (NEB). For this ...
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bioRxiv - Biochemistry 2024Quote: ... were PCR amplified with indicated primers (see Table S1) and inserted into backbone plasmids via restriction digest and ligation (all enzymes from NEB) or Gateway cloning (Thermo) ...
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bioRxiv - Bioengineering 2024Quote: ... And cloned into a expression plasmid under the control of the EF1α using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs). To generate 3’ and 5’ stop codon reporters ...
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bioRxiv - Bioengineering 2024Quote: ... Digested backbone plasmid and annealed spacer sequences were assembled using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs) in a reaction containing 5 µl of the master mix 2 µl of the digested backbone and 3 µl of the PCR annealed spacer ...
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bioRxiv - Genetics 2023Quote: ... and incubated with restriction enzyme-digested CD510-B1 plasmid (SystemBio) and assembled with Gibson Assembly Master Mix (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... The amplicons were digested with SacI and EagI ligated into the plasmid containing the ATG8 promoter linearized with HindIII (New England Biolabs) and SalI (New England Biolabs) ...
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bioRxiv - Genomics 2024Quote: ... Gibson assembly reactions to create these plasmids were performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, #E2621L).
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bioRxiv - Genomics 2024Quote: ... and the wild-type (USH2A:c.7595-2144A) minigene plasmids were generated by Q5® High-Fidelity DNA Polymerase (New Englands Biolabs) amplification of the target USH2A region using gDNA of a human heterozygous USH2A:c.7595-2144A/G patient and subsequent cloning into the pSPL3 backbone vector by NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were then introduced into pBBR1-MCS2 plasmid at XhoI and HindIII restriction sites (#R0146 and #R0104; New England Biolabs NEB). We then transformed (thermal shock ...
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bioRxiv - Genomics 2024Quote: ... The purified scaffold insert (2 ng) was ligated with the digested intermediate plasmid library vector (200 ng) using T4 DNA Ligase (NEB) at room temperature for 45 min ...
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bioRxiv - Genetics 2024Quote: The ligation reaction was performed in a 1:5 plasmid to insert copy number ratio using T4 DNA ligase (NEB) at 16°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Examples of plasmid DNA with similar profiles were additionally differentiated by digestion with restriction enzymes BamHI and HindIII (NEB, USA). The digestion was performed according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2024Quote: Plasmids were constructed by amplifying inserts by PCR using specific oligonucleotides and the Q5 high fidelity enzyme (New England Biolabs). PCRs were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: Plasmid pET28bR109H for overexpression of the R109H mutant was obtained by site-directed mutagenesis of plasmid pET28bRho (kindly provided by Pr. James Berger, Johns Hopkins University) using the NEBuilder HiFi kit (New England Biolabs). The R109H mutant was overexpressed in BL21(DE3)pLysS cells carrying the pET28bR109H plasmid and purified as described for WT Rho 105.
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bioRxiv - Microbiology 2021Quote: ... reporter genes (obtained as gBlock Gene Fragments from IDT) were then ligated into the linearized plasmid by Gibson Assembly (NEBuilder® HiFi DNA assembly, NEB). The phosphoprotein gene start and the hemagglutinin (H ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a donor DNA containing two homology arms flanking the DsRed sequence for homology repair were cloned into the pHD-ScarlessDsRed plasmid using Q5® High-Fidelity DNA Polymerase (New England Biolabs). Left homology arm contained the sequence in 3R:16188287-16189463 ...
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bioRxiv - Molecular Biology 2019Quote: ... of pA-Dam protein was incubated with 500 ng of unmethylated plasmid in 20 µL of 1x dam MethylTransferase buffer (New England BioLabs #M0222S) supplemented with 80 µM S-adenosylmethionine (SAM ...
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bioRxiv - Microbiology 2021Quote: E248R deletion mutant proteins of distinct domains (Δ constructs) were generated from E248R WT plasmid by site direct mutagenesis using the Q5 mutagenesis kit (New England Biolabs) as follows ...
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bioRxiv - Genetics 2021Quote: All constructs used for homologous recombination into the chromosome were constructed in a pUC19 plasmid vector (New England Biolabs, Ipswich, MA). PCR was used to amplify DNA fragments and Gibson assembly cloning (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified PCR products were ligated into the recombinant plasmid pZE0-P-MglE-T using the recommended standard USER cloning protocol (NEB, UK).
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bioRxiv - Molecular Biology 2020Quote: ... and the next two fragments were inserted into resulting plasmid using NEBuilder®HiFi DNA Assembly Cloning Kit (New England Biolabs). Fragments were amplified with primers (Table S1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mcr-1 gene along with its natural promoter was PCR-amplified from the natural PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified and purified mcr-1 fragment was assembled together with PCR-amplified pSEVA121 backbone using NEBuilder® HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The resulting fragment is then ligated into a digested pCDNA3-YFP Basic plasmid that had been cut with Kpn1 and Xho1 using a standard T4 DNA Ligase protocol (NEB #M0202). Once the insert ...
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bioRxiv - Molecular Biology 2021Quote: TCF20(1-1268)-mEGFP construct was generated from the TCF20-mEGFP plasmid using the Q5 Side-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: All single gRNA-expressing plasmids were assembled by Gibson assembly reaction using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). Depending of the plasmid features ...
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bioRxiv - Genetics 2019Quote: ... and integrated with hygromycin resistance cassette amplified from plasmid pUMa1507 (Terfrüchte et al. 2014) by Gibson Assembly (New England Biolabs, Ipswich, USA).
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bioRxiv - Cell Biology 2019Quote: ... red fluorescent protein coding sequence (dsRed) were cloned into the above derivative tetracycline inducible plasmid with NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) at NheI and MluI restriction sites (pLVD-GRASLND and pLVD-dsRed) ...
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bioRxiv - Neuroscience 2019Quote: ... and the coding sequence of TurboRFP fused to the 3’ UTR of the ribosomal gene Ppa-rpl-23 (Schlager et al., 2009) were cloned into the pUC19 plasmid using Golden Gate Assembly Mix (New England BioLabs, E1600S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: IVT was performed using the T7 promoter of the pcDNA3.3_NDG plasmid and HiScribe T7 ARCA mRNA kit with tailing (NEB #E2060S, Ipswich, MA). Whole kit was used with 20 ug DNA following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Donor sequences which typically contain 500-bp upstream and 500-bp downstream of the editing sites were amplified by PCR and ligated into the linearized targeting plasmid (digested by XhoI (NEB, USA)) using the ClonExpress One Step Cloning Kit (Vazyme ...
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bioRxiv - Genetics 2019Quote: ... as shown in figure S2) were introduced into the ARSA plasmid by using Site-Directed Mutagenesis Kit (Cat. No. E0552s, NEB, USA). The sequences of mutated cDNA vectors were confirmed using the ABI3500 sequencer (Applied Biosystems Inc. ...
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bioRxiv - Microbiology 2021Quote: ... the SAG1 3’UTR was amplified from pNJ-26 and cloned into the tagging plasmid to replace DHFR 3’UTR by Gibson assembly (NEB, E5520S). BAG1-mCherry GCaMP6f reporter tachyzoites were co-transfected with 10 μg of pSAG1::CAS9-U6::sgDHFR 3’UTR and 2 μg of PCR amplified P2A-mTagBFP2-HXGPRT flanked with 40 bp homology regions ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations to the binK sequence on the plasmid were designed using the NEBaseChanger tool and constructed with the Q5 site-directed mutagenesis kit (New England BioLabs, Inc.). The constructed plasmid was transformed into either electrocompetent or chemically competent DH5α λpir E ...
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bioRxiv - Bioengineering 2021Quote: ... homology arms were amplified from genomic DNA and introduced to the MCS of the linearized SIBR-Cas plasmid using the NEBuilder® HiFi DNA Assembly Master Mix (NEB). The plasmid was linearized using Esp3I (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... 30 bp at the 3’ end of PAM site was selected as the guide sequence and cloned into pCASCADE plasmid using Q5 site-directed mutagenesis (NEB, MA) following manufacturer’s protocol ...