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Citations for New England Biolabs :
1801 - 1850 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... reverse: 5’-GATGGCGTGGAACCATGTC-3’) were obtained from the wild type plasmids pCMV-hnCoV-S via Q5 SiteDirected Mutagenesis Kit (NEB). pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward ...
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bioRxiv - Molecular Biology 2021Quote: ... Gibson assembly ligation into a restriction-enzyme digested pCAG backbone plasmid was then performed using Gibson Assembly Master Mix (NEB) according to manufacturer’s guidance.
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bioRxiv - Microbiology 2019Quote: ... The pBAMD1-4 plasmid was linearized by digestion with HindIII-HF and EcoRI-HF (New England Biolabs # R3104S and # R3101S) at 37 °C for 2 h in Cutsmart buffer ...
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bioRxiv - Microbiology 2020Quote: ... Gel extracted DNA fragments were incubated in a ratio of 1:3 of plasmid to insert (1:5 for inserts smaller than 300 nucleotides) in the presence of Gibson Assembly master mix (NEB) at 50°C for 1 hour.
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bioRxiv - Microbiology 2020Quote: ... All CRISPR/Cas9 plasmids used in this study were derived from the single-guide RNA (sgRNA) plasmid pSAG1:CAS9-GFP, U6:sgUPRT (Shen, Brown et al. 2014) by Q5 site-directed mutagenesis (New England Biolabs) to alter the 20-nt sgRNA sequence ...
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bioRxiv - Microbiology 2019Quote: ... cassette between SalI and BglII in the plasmid pGDB34 was isolated by digestion and gel purification and inserted between the same restriction sites in the plasmid pyPM2GT-EXP2-mNeonGreen14 with a T4 Quick Ligation kit (NEB), replacing the yDHODH cassette ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids(von Boehmer et al., 2016) using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... σ3 WT and mutant sequences flanked by BamHI and EcoRI sites were synthesized commercially by ATUM and then cloned into pWPXL-GFP plasmids to replace GFP sequence through T4 ligation (NEB). pWPXL-Flag and pWPXL-HA vectors were generated by ATUM by replacing GFP with Flag- or HA-‘stuffer sequences’ flanked by BamHI and EcoRI sites ...
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bioRxiv - Immunology 2020Quote: ... The linearized plasmids were purified and utilized as templates in a T7 ARCA in vitro transcription reaction (New England Biolabs). The mRNA product was then purified using an Invitrogen Purelink RNA mini kit according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... A 2-micron Ura3-selective plasmid was constructed from three DNA fragments using HiFi DNA Assembly Master Mix (New England Biolabs) for cloning ...
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bioRxiv - Developmental Biology 2021Quote: ... each CBE4max-SpG and CBE4max-SpRY sequence has been inserted into pCS2+ plasmid linearized with EcoRI using the Gibson Assembly Cloning Kit (New England Biolabs). The fragment has been amplified using the primers F-5’-TGCAGGATCCCATCGATTCGGCCACCATGAAACGGACAG -3’ and R-5’-TAGAGGCTCGAGAGGCCTTGTCAGACTTTCCTCTTCTTCTTGG -3’ ...
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bioRxiv - Cell Biology 2021Quote: ... S.aureus Cas9 was amplified with homologous adaptors from pX601 by PCR for ligation into the pMB950 plasmid digested with NheI and XhoI (New England Biolabs).
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bioRxiv - Genetics 2020Quote: ... The ‘2A-Puro-WPRE’ sequence was then removed from the LCv2 plasmid via restriction digestion with PmeI (NEB, Cat# R0560S) and BamHI (NEB ...
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bioRxiv - Genetics 2020Quote: ... the ‘T2A-GFP-WPRE’ sequence was amplified from the hVMD2-hBEST1-T2A-GFP plasmid using LCv2-GFP.Gib.F and .R primers and Q5 2X MM (NEB, Cat# M0492L). The ‘2A-Puro-WPRE’ sequence was then removed from the LCv2 plasmid via restriction digestion with PmeI (NEB ...
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bioRxiv - Genomics 2021Quote: The pCeTyX Ty1hygro-AI carrying plasmid was constructed by assembling four overlapping DNA fragments thanks to the Gibson Assembly Cloning Kit (NEB). The 5,472 bp plasmid backbone was obtained after restriction of p41Neo 1-F GW (https://www.addgene.org/58545/ ...
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bioRxiv - Biophysics 2021Quote: ... The R525E-R527E mutant plasmid was generated using pET-24a-XccBphP as a template for mutagenesis using whole plasmid amplification followed by DpnI template digestion (New England Biolabs). This was achieved using Q5 High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... PCRs purified using the Nucleospin gel and PCR clean-up kit were cloned into plasmid backbones digested with restriction enzymes (NEB) using the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Developmental Biology 2022Quote: ... HDR plasmid with mutant me31B genes was generated by mutating the me31B wild type gene in the wildtype HDR donor plasmid by using the Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturer’s recommended protocols ...
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bioRxiv - Cell Biology 2022Quote: MKLP1(1-711)-3xFLAG-Avi was cloned by stitching four oligonucleotide primer sequences together into a digested MKLP1(1-711)-Avitag plasmid using a HiFi DNA assembly reaction kit (M5520A, New England Biolabs). Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2022Quote: ... RD fragments amplified from Drosophila embryonic cDNA were integrated between SgrDI (Thermo) and BsHTI (Thermo) restriction sites in the respective backbone plasmid via Gibson assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The new construct was created from a PCR reaction that amplified the entire plasmid harbouring the substitution using the high-fidelity polymerase Q5 according to the manufacturer’s instructions (New England Biolabs; NEB). The unpurified PCR product was then treated with DpnI to digest the methylated template DNA which was subsequently transformed into DH5α ...
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bioRxiv - Biophysics 2022Quote: ... the assembled sequences were transferred to the target plasmids (WT, MUT) by using a different set of type IIS enzymes (BbvI, New England Biolabs #R0173 ...
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bioRxiv - Bioengineering 2022Quote: ... A 4699 bp HDR template dsDNA (HDRT) was generated by amplification from a plasmid template using the Q5 High-Fidelity 2X Master Mix (New England BioLabs) with primers containing truncated Cas9 target sequences (IDT ...
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bioRxiv - Bioengineering 2022Quote: All rAAV6 vector plasmids were cloned using NEBuilder® HiFi DNA Assembly Master Mix (cat # E2621L, New England Biolabs (NEB) Inc. ...
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bioRxiv - Bioengineering 2022Quote: All rAAV6 vector plasmids were cloned using NEBuilder® HiFi DNA Assembly Master Mix (cat # E2621L, New England Biolabs (NEB) Inc. ...
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bioRxiv - Biochemistry 2022Quote: Elp constructs were PCR amplified with encoded 5’ BamHI and 3’ NotI restriction sites from the previously generated full-length ElpQ19-343 and ElpB19-378 pT7HMT plasmids using Q5 Master Mix (NEB) as previously described (31) ...
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bioRxiv - Cell Biology 2022Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
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bioRxiv - Immunology 2022Quote: Protein expression plasmids were constructed using dsDNA gBlocks from IDT and NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, E5520S) and then transformed in NEB 5-alpha competent cells (NEB ...
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bioRxiv - Genetics 2022Quote: ... I23A was introduced at the same time with GFP knock-in by incorporating the corresponding mutation in the 3’ homology arm on the repair template plasmid using the Q5 site-directed mutagenesis kit (New England Biolabs). GermLine Optimized mScarlet-i sequence (Fielmich et al ...
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bioRxiv - Genomics 2022Quote: ... Ligand-encoding sequences were subsequently cloned by restriction digest removal of CD19-encoding sequence from the EF1ɑ-CD19 IRES-EGFP lentiviral plasmid using XbaI & MluI (New England Biolabs) and inserted with InFusion cloning (Takara Bio) ...
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bioRxiv - Genomics 2022Quote: A second-generation lentiviral transfer plasmid encoding expression of EF1ɑ promoter - CAR-P2A-mCherry (21) was digested with XbaI & MluI (New England Biolabs) to drop out the CAR-P2A-mCherry transgene ...
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bioRxiv - Immunology 2022Quote: Fab fragments were generated by inserting a stop codon six amino acids upstream of the hinge region (CPPCP) of the heavy chain expression plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). This mutagenized plasmid was co-transfected with the respective light chain plasmid ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 40bp downstream) were PCR-amplified from the PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified fragment was cloned into pSEVA121 using the NEBuilder® HiFi DNA Assembly kit (New England BioLabs ...
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bioRxiv - Genomics 2022Quote: PCR templates for in vitro transcription (IVT) were amplified from S2 cell cDNA or pGF-P5(S65T) plasmid using Phusion Hot Start Flex DNA Polymerase (NEB) and primers listed in Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Other G2L4 and GII RT mutant plasmids were derived from pMal-G2L4 RT or pMal-GII RT by using a Q5 mutagenesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... The PCR was done with 1-2 ng of plasmid and 200 nM of each primer in Phusion High-Fidelity PCR Master Mix (New England Biolabs) with pre-denaturation at 98°C for 5 sec followed by 12 cycles of 98°C for 5 sec ...
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bioRxiv - Microbiology 2022Quote: ... These vectors were generated by mutating the sgRNA site in the pSag1-Cas9-U6-sgUPRT-HXG plasmid [14] using the Q5 Site-Directed Mutagenesis Kit (NEB). The two guide RNAs were designed to target TgEFP1 at either of the predicted EF-hand domains using the online E-CRISP tool (http://www.e-crisp.org/E-CRISP/) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid backbones and inserts for cloning were amplified from the purified chromosomal DNA using Q5 High-Fidelity DNA Polymerase (NEB). PCR products and plasmids were purified with Monarch DNA kits (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... The other molecular cloning trials and plasmid gene replacements were performed by Gibson’s assembly with Gibson Assembly® Cloning Kit (NEB). The procedure used to generate each plasmid construct is described in the corresponding subsection of Material and Methods.
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bioRxiv - Microbiology 2022Quote: ... followed by gel-purification of the plasmid backbone and Gibson assembly with re-targeted intron fragments using the Gibson Assembly Master Mix (NEB). Gibson assemblies were transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... Multilayer genetic circuit plasmids were constructed using PCR and Golden Gate assembly (GAA) using BsmBI Type IIS Enzyme (New England Biolabs), and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... The fragments were purified and inserted into pCAMBIA3300U (pCAMBIA3300 with a double PacI USER cassette inserted between the PstI-XmaI sites at the multiple cloning site) (Nour-Eldin et al, 2006) after which the plasmid was transformed into Escherichia coli DH5α (NEB). Kanamycin resistant colonies were selected and their plasmids purified followed by restriction digestion and sequencing prior to introduction into Agrobacterium tumefaciens GV3101 for plant transformation ...
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bioRxiv - Neuroscience 2022Quote: ... The digested plasmid and the PCR product were then assembled with NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs). Chemically competent Stbl3 Escherichia coli cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: 5’ biotinylated DNA that was used for transcription antitermination assays and as a PCR template when preparing modified DNA templates below was PCR amplified from plasmid DNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and primers HP4_5bio.R and PRA1_NoMod.F (for ZTP and fluoride templates ...
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bioRxiv - Plant Biology 2022Quote: ... The sgRNA expression cassettes of OsU6c–sgRNA–T1 and OsU3–sgRNA–T2 were amplified from pYLsgRNA–OsU6c and pYLsgRNA–OsU3 plasmids using Phusion High-Fidelity DNA Polymerase (New England BioLabs) and cloned into a binary vector ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplified yciF (with Strep II tag on C-terminal) and pQE60 plasmid (containing an IPTG inducible T5 promoter) were digested with BamHI and EcoRI (New England Biolabs) and purified by gel extraction (MinElute gel extraction kit ...
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bioRxiv - Genomics 2022Quote: All plasmids were constructed using standard cloning methods with New England Biolabs restriction enzymes and T4 DNA ligase (New England Biolabs) or with the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Microbiology 2022Quote: ... FastDigest restriction enzymes (Thermo-Fisher) were used for plasmid digestion and Gibson assembly was performed using the NEBuilder HiFi DNA assembly kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Synthetic Biology 2022Quote: A double-stranded DNA scaffold template was obtained from pUCIDT-AMP-T7p_DB982 plasmid (10 ng in 50 μL reaction mixture) by PCR amplification using Phusion® DNA polymerase (NEB), T7-DB982 forward and T7-DB982 reverse primers (0.5 μM each) ...