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Citations for New England Biolabs :
2201 - 2250 of 2416 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Both purified genes and plasmids were digested with Nde I and Not I prior to ligation using the T4 DNA ligase (New England Biolabs, UK). The recombinant plasmids pET-hGFAT2 (without tag ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into pNL4-3 plasmid following digestion with BssHII and SalI (New England Biolab, USA) and ligation using T4 DNA ligase (New England Biolabs, USA). The chimeric viruses were produced by transient transfection of the plasmids into the 293T cell line using FuGene HD and harvested 72 hours later by a collection of the cell-free supernatant cleared by centrifugation and stored in aliquots at −80°C.
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CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsbioRxiv - Genetics 2020Quote: ... was first cloned into the pKOPIS plasmid (Kuang et al. 2018) using the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) (Hsieh 2018) ...
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bioRxiv - Cell Biology 2021Quote: ... containing TauRD-I260C for fluorescent labeling were constructed by mutagenesis of the pHUE-TauRD (P301L/V337M) plasmid using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2020Quote: ... The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB, R3101S) and AgeI (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 pg of lhx1a:DsRed/pI-SceI plasmid DNA was injected into 1-cell stage embryos along with the I-SceI restriction enzyme (NEB, R0694S). These injected embryos were raised to adulthood and screened for DsRed expression in known lhx1a expression domains and isolated a stable Tg(lhx1a:DsRed ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription templates were amplified from the plasmids by PCR and and transcribed in vitro with the HiScribe T7 High Yield RNA synthesis kit (New England Biolabs Inc.). To obtain sufficient amounts of mRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... cells we first created the pHAGE-empty plasmid (pAS4940) by excising the ERBB2 coding sequence from pHAGE-ERBB2 (addgene #116734) using Xho1 (NEB, R0146S) followed by re-ligation of the vector ...
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bioRxiv - Cell Biology 2020Quote: ... Two missense point mutations present in the initial plasmid (c1411t and c2671t) were corrected using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S) according to manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... The seven PCR products were assembled into the pCC1-F567-mNG plasmid that were pre-linearized with NheI and XhoI by using the NEBuilder® HiFi DNA Assembly kit (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The 3-part assembly reaction (plasmid-promoter-insert) was performed using the Gibson assembly master mix 2x (New England Biolabs #E2611S), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme digest of 500 ng of plasmid DNA extracted from 190693 and 169757 was performed with PpuMI and XhoI (both New England Biolabs, USA) and immediately run on a 1% agarose gel following incubation for 1 hour at 37°C.
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bioRxiv - Molecular Biology 2021Quote: Site-directed mutagenesis of the AP-1 binding sites in the distal enhancer reporter plasmids were performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Primer pairs 5’-CCATAATGTGgggCTATACTAAATTTCATCTTC-3’ and 5’-CTAAATCCACTTAGAAAAAACAATC-3’ ...
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bioRxiv - Genetics 2020Quote: ... The digestion product was resolved on a 0.7% agarose gel and the plasmid backbone was purified using the Monarch gel purification kit (NEB, Cat# T1020S). The ‘T2A-GFP-WPRE’ sequence was inserted into the digested backbone using the Gibson Assembly kit (SGI ...
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bioRxiv - Genomics 2021Quote: ... Gibson Assembly was performed using the amplified plasmid backbone and a synthesized DNA fragment “GibsonBC4” according to the manufacturer’s protocol (NEB cat# E2611L). Barcoded plasmid library DNA was purified using the Zymo Clean and Concentrate-5 (Zymo Research cat# D4014 ...
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bioRxiv - Molecular Biology 2021Quote: ... 500bp upstream and 500bp downstream sequence from start codon was inserted in pFA6a-kanMX6 plasmid [60] after the kanMX6 cassette using Gibson assembly cloning (NEB, E2611). FRB tag [23] was then inserted after the start codon by using Gibson assembly cloning and used as template for PCR ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding ORFs were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and SphI enzymes (New England Biolabs, USA) (ECP_3022 ...
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bioRxiv - Biophysics 2022Quote: ... Both insert and vector were digested using the said restriction enzymes and ligated to form a circular plasmid using T4 DNA ligase (NEB, #M0202L). Sequence was verified by sanger sequencing.
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bioRxiv - Cell Biology 2022Quote: All plasmids reported in this work were constructed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Cat. #E2621), based on the Gibson assembly method (Gibson et al. ...
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bioRxiv - Cell Biology 2022Quote: ... the PCR product and the pDRF1-GW plasmid were digested using BamHI-HF and NheI-HF (New England Biolabs, Ipswich, MA) and the PCR product was ligated into the plasmid using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Immunology 2022Quote: Point mutations were introduced through linearizing the plasmid by PCR with primers containing the intended mutations (Supp. Table S1) followed by reverting to circular plasmid with Gibson Assembly Master Mix (M5510A, NEB, USA).
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bioRxiv - Microbiology 2023Quote: An integration cassette containing two regions of homology flanking a kanamycin-resistance gene was engineered on a pUC backbone plasmid encoding ampicillin resistance using the NEBuilder HiFi DNA Assembly mastermix (NEB E2621L). A counter-selectable marker based on PheS77 was engineered under the constitutive SpeI promoter and cloned into the pUC plasmid (generating pUC-BC ...
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bioRxiv - Molecular Biology 2022Quote: RLuc-P2A-X-P2A-Fluc plasmids (where X represents a variable sequence) were assembled using NEB Builder HiFi DNA Assembly Cloning Kit (New England Biolabs, #E2621S) by combining the original plasmid digested with HindIII (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...
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bioRxiv - Neuroscience 2022Quote: ... and was ligated with 500 ng of the purified digested plasmid band and then transformed in NEB stable competent cells (New England BioLabs, C3050H). Successful ligation of the guide was confirmed by Sanger sequencing using a mU6 sequencing primer (Supplementary Table 7).
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bioRxiv - Physiology 2022Quote: To identify the molecular lesion in the hyds-2 strain a 9KB PCR fragment of the ZK795.1 gene was cloned into the PUC19 plasmid by Gibson assembly (New England Biolabs, Ipswich, MA). The seaEx22 plasmid along with a myo-2:mCherry (PCFJ90 ...
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bioRxiv - Bioengineering 2024Quote: ... Primers containing the spacer sequence and homology to the rcRNA plasmid backbone were annealed using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA fragments for construction of the plasmids were obtained through either high-fidelity amplification using Q5 DNA Polymerase (NEB, USA) or restriction enzyme digestion ...
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bioRxiv - Microbiology 2024Quote: nfsA and nfsB mutants were constructed by gene inactivation using the pKNOCK suicide plasmid (22) The DNA fragments were amplified with Phusion high-fidelity DNA polymerase (NEB, UK) from E ...
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bioRxiv - Microbiology 2024Quote: ... All recombinant plasmids in this study were constructed with NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts, USA). All oligonucleotides were ordered and all sequencing works were done at Eurofins Genomics (Ebersberg ...
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bioRxiv - Molecular Biology 2024Quote: ... the linear DNA was digested using 28.8 U/μL of plasmid-safe ATP-dependent DNase Exonuclease V (ExoV, RecBCD) (New England Biolabs, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were then introduced into pBBR1-MCS2 plasmid at XhoI and HindIII restriction sites (#R0146 and #R0104; New England Biolabs NEB). We then transformed (thermal shock ...
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bioRxiv - Microbiology 2024Quote: The pLIC-HA-DHFR-TS (gift from Jeroen Saeij, University of California, Davis) plasmid was treated simultaneously with PacI (NEB #R0547S) and AvrII (NEB #R0174S ...
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bioRxiv - Microbiology 2024Quote: ... A fragment containing the CRISPR array plus the extra BsrG1 restriction site was cut from the synthesized pUC19 by digestion with DraI and BstZ17I enzymes and cloned into the BsaI-linearized pSTU-1 plasmid using the NEBuilder HiFi cloning kit (New England Biolabs, UK). The plasmid sequence was confirmed by sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... were digested with NotI and the wc-1 and wc-2 fragments were cloned into the respective plasmids with NEB HiFi DNA assembly mastermix (NEB, US). This resulted in the prey plasmid wc-1-pMB29 and bait plasmid wc-2-pMB28 ...
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bioRxiv - Cell Biology 2023Quote: Capped mRNAs with poly-A tail were synthesized from linearized pGEMHE plasmids using the HiScribe™ T7 ARCA mRNA Kit (NEB). The mRNA was purified by Monarch RNA Cleanup Kit (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The Homology-Directed Repair (HDR) template used for gene replacement was assembled into plasmid pBluscript KS-using HiFi assembly (NEB, E2621S). Two independent mutants were obtained based on two different protoplast pools and different sgRNAs (Supplemental Table 1) ...
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bioRxiv - Systems Biology 2023Quote: ... HiFi-Cas9 and LZ3-Cas9 constitutive expression plasmids lentiHiFi-Puro and lentiLZ3-Puro were constructed by the Gibson Assembly Site-Directed Mutagenesis approach (NEB, #E2621) using lentiCas9-Puro as the template ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified barcode fragment was then assembled into the cloning backbone plasmid pKI110 by Golden Gate Assembly (66) using BsmBI (NEB #R0580S). We performed two assembly reactions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the plasmids for the Blasticidin and Zeocin resistance marker-based systems were introduced to T7 Express chemically competent cells (NEB #C2566I) according to the manufacturer’s high-efficiency transformation protocols ...
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bioRxiv - Synthetic Biology 2023Quote: ... Linear plasmid fragments were generated by PCR and purified by Monarch® PCR and DNA cleanup kit (New England BioLabs®). Ligations were performed with In-Fusion® enzyme at 50°C for 15 minutes (min ...
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bioRxiv - Synthetic Biology 2023Quote: ... we performed the ligation of fragments from module plasmids in the presence of BsaI-HF (New England BioLabs, Ipswich, MA, USA) to generate array plasmids ...
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bioRxiv - Microbiology 2022Quote: ... Fragments were integrated into the linearized (KpnI/XbaI digested) pRK415 plasmid by NEBuilderR HiFi assembly cloning method (NEB Lab, United States) as described above.
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bioRxiv - Molecular Biology 2022Quote: PANK3 mutations were made in a gateway compatible pDONR plasmid containing a PANK3 ORF (a gift from the lab of Ben Cravatt) by amplifying the whole plasmid with primers containing the desired mutations and using HiFi DNA Assembly Master Mix (NEB, # E2621) to re-circularize the amplicon ...
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bioRxiv - Microbiology 2023Quote: ... Phosphorylated primers containing one half of the barcode each at the 5’ ends were used to linearize the pUC-BC plasmid and the resulting amplicon was ligated using T4 DNA Ligase (NEB M2200L). The purified ligation reaction was transformed into NEB-10-Beta competent cells (NEB C3020K ...
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bioRxiv - Microbiology 2023Quote: Plasmid construction was performed by: PCR amplification of the fragment to insert in the plasmid with Q5® High-Fidelity DNA Polymerase (New England Biolabs), digestion with the appropriate FastDigest restriction enzymes (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... All enzymes for DNA amplification (PHUSION® polymerase) and plasmid construction (restriction enzymes, T4-DNA ligase and Quick CIP) were obtained from New England Biolabs (NEB) and used according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... with primers listed in Table S8, then inserted into PHR-mCh- CryWT plasmid (Adgene, 101221) by using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S). The generated constructs were fully sequenced to confirm the absence of any mutations or stop codons ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of linearized plasmid was used as template in a 10 µL reaction using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) with an 8:1 ratio of cap analog to GTP ...