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Citations for New England Biolabs :
2001 - 2050 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Reverse transcription of RNA to cDNA was performed by combining 500 ng/ mL of the RNA samples with the LunaScript™ RT SuperMix (5X) (NEB) in 20 μL aliquots ...
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bioRxiv - Microbiology 2020Quote: ... After adding 10% 1M sodium dodecyl sulphate (SDS) and 10 μL of 8 U/mL proteinase K (NEB, #P8107S, Ipswich, MA), the suspension was then incubated at 56°C for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μl of the diluted samples was then denatured by addition of 4 M urea and Proteinase K (40 U/ml; New England Biolabs #P8107S), incubated for 5 min at 65°C ...
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bioRxiv - Biochemistry 2022Quote: ... the used RNA was radioactively labelled on the 5’-end with [γ-32P]ATP (10 mCi/ml, Hartmann Analytic) and T4 PNK (NEB), following purification via PAGE ...
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bioRxiv - Microbiology 2021Quote: The fusion protein was purified from the clarified supernatants by adding 20 ml of chitin resin (New England Biolabs, cat#S6651S) and incubating with gentle rotation overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... We added 1 mL of RAD reagent from the RAD002 kit and 0.2mL of Blunt TA ligase (New England Biolabs, MA, USA) and the reaction was incubated for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... and 50 µg/ml Proteinase K) at 65 °C for 12 hrs and purified with Monarch DNA Cleanup Columns (New England Biolabs, T1030L). For ALaP-qPCR analysis ...
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bioRxiv - Biochemistry 2020Quote: ... chromatin and chromatin bound fractions were released from magnetic beads using binding buffer containing 5 mM CaCl2 plus an excess (2000 units/ 20 mL reaction) of micrococcal nuclease (MNase; NEB, M0247S) Beads were incubated for 5 minutes at 37 °C with shaking (1250 rpm) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with PBS and the cells were incubated in 1ml serum free medium and 250U/ml PNGase F (NEB, P0704S) for 6h ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome remodeling reactions (25 μL) were carried out in REA Buffer with 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Microbiology 2021Quote: SYBR-based qPCR reactions were set up in duplicate as follows: 25 μl reactions with 20 U/ml Phusion Hot Start Flex DNA polymerase (NEB M0535L), 1X HF Buffer ...
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bioRxiv - Immunology 2020Quote: ... For genotyping hair samples were incubated in 250 μL of Direct PCR reagent and 20 mg/mL Proteinase K (New England Biolabs, P8102) for 1 h at 55°C ...
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bioRxiv - Molecular Biology 2022Quote: ... MNase dilutions were prepared fresh before each digestion at 25 μg/mL in 1X bovine serum albumin (BSA) (New England Biolabs B9001S).
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bioRxiv - Cell Biology 2022Quote: ... Dephosphorylation of CRL4B took place in a 100 μl reaction mixture containing 86 μl of CRL4B protein (at 0.8 mg/ml) with 2 μl λ-phosphatase (λ-PP) in the presence of 0.1 mM MnCl2 and 1x PMP reaction buffer (NEB). Untagged-CRL4 complexes (4A ...
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bioRxiv - Genomics 2022Quote: ... 1500 calcein-stained cells in 5 μL of were added to 35 μL of barcoded hydrogel templates with 29 U/mL Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Genomics 2024Quote: ... Samples were then washed twice with PBS-T and incubated for 15 minutes with 20 µg/mL Streptavidin (New England Biolabs N7021S) and 100 µg/mL rAlbumin (New England Biolabs B9200S ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5XSSC in H2O) on a light table for 1 hour and treated with 2 μg/mL Protease K (NEB, Cat PB107S) in PBS containing 0.3% Triton-X for 10 minutes followed by post-fixation in 4% PFA for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Template DNA was digested with the addition of 1 μL RNase-free DNaseI (NEB # M0303S; enzyme stock at 2,000 U/mL) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation at 214,743 rcf at 4°C in S55A rotor using a Sorvall Discovery M120 SE Micro-Ultracentrifuge and added to 1 mL of Chitin Resin (NEB # S6651S) in a Peirce centrifugation column (Thermo Fisher # 89897 ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 at room temperature for two hours in the presence of 25 mU ml-1 apyrase (NEB, cat. no: M0398S) and either C3a or EP54 for complex formation ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM MgCl2 and 1 mM DTT) and treated with 0.5 U/mL calf intestinal alkaline phosphatase (New England Biolabs, cat#M0290) in dephosphorylation buffer for 10 minutes at 37 ℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... 1–10 µg of RNA transcribed in vitro was treated with Escherichia coli poly(A) polymerase (5000 U/mL, New England Biolabs, M0276) at 37°C for 30 min followed by clean-up using an RNeasy Mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... were grown out in LB medium with 50 µg/mL Kanamycin and then pelleted before plasmids were isolated using the Monarch Plasmid Miniprep Kit (NEB, T1010L). Plasmids were sequenced (Sanger ...
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bioRxiv - Genetics 2023Quote: ... at 37°C overnight while shaking to digest chromatin, then ligation incubation buffer (10mg/mL BSA, 1x T4 DNA Ligase Reaction Buffer (NEB, #B0202S) and 2000U of T4 DNA Ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Digestion products were diluted by the addition of 4 mL 1.1× T4 DNA Ligase Reaction Buffer (New England BioLabs® Inc) with 1% Triton X-100 and incubated for 1 h at 37°C ...
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the resulting cell pellet was resuspended in 1 mL of RNA Protection Buffer (1× NEB DNA/RNA Protection Reagent, 1% (w/v) polyvinylpyrrolidone-40 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.5 µl of 1 mg mL−1 purified bovine serum albumin (1:20 dilution in dH2O of BSA, Molecular Biology Grade 20 mg ml−1, NEB cat. B9000), 0.25 µl of T4 DNA ligase at 400 U µL−1 (NEB cat ...
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bioRxiv - Genomics 2020Quote: ... we heated samples to 95°C for 3min and snap-cooled on an ice slush before gently adding 0.5μl of enzyme solution (8,000 U/ml Bst DNA Polymerase Large Fragment, New England Biolabs, USA, in 1X amplification buffer) into the aqueous droplet.
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bioRxiv - Biochemistry 2021Quote: ... 50 μg/ml glycogen) and DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, T1030). About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103) ...
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... samples were washed in phosphate buffered saline (PBS) and labelled with 1:500 of 10mg/mL Hoechst 33342 (New England Biolabs, Cat# 4082S) for 10 min with a subsequent wash in PBS ...
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bioRxiv - Microbiology 2021Quote: ... resulting in ∼1 ml of turbid bacterial culture which was subsequently used as input for plasmid purification by miniprep kit (New England Biolabs, cat#T1010S). The pooled plasmid library DNA for each library was eluted in 35 μl of elution buffer and quantified ...
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bioRxiv - Biophysics 2021Quote: ... The surface is then passivated by incubating bovine serum albumin (BSA, New England Biolabs, 10 mg/ml in PBS and 50% glycerol) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Biochemistry 2021Quote: ... to 10 μl of the plasma/stop solution 70 μl of mixture consisting of 75 mU/ml ATP sulfurylase (New England Biolabs, Cambridge, MA), 1 μmol/L APS (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR product were circularized by intra-molecular self-ligation in 1 ml of ligation buffer using T4 DNA ligase (New England Biolabs, Ipswich, MA). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in 1 mL ice-cold RNase-free cell lysis buffer supplemented with 1 μL RNase inhibitor (M0307S, NEB; Ipswich, MA). After storage in −80°C refrigerator for about 40 mins ...
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bioRxiv - Molecular Biology 2022Quote: ... Additional reconstitutions with linker histone were performed by mixing reconstituted 183×12 core arrays with 1 mg/ml recombinant linker histone variant H10 (New England Biolabs, cat.# M2501S) at a molar ratio of 0.7 molecule histone H1 per nucleosome in solution containing 500 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... and the impurities that were tagged by Chitin Binding Domain were extracted on a gravity-flow column using 5 mL Chitin resin (New England BioLabs, Evry, France). The target proteins were purified with HisTrap HP columns (GE Life Sciences ...
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bioRxiv - Genomics 2022Quote: ... samples were gently removed from the chamber and digested overnight at 37°C in 8 U mL-1 proteinase K (NEB, cat# P8107) in digestion buffer (1× TAE buffer ...
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bioRxiv - Neuroscience 2022Quote: ... or medium that were pulled down with heparin-agarose (0.5 ml) were denatured and incubated with Arthrobacter ureafaciens sialidase (Nacalai Tesque, 4 milliunits) and/or O-glycosidase (New England BioLabs, 80,000 units), or peptide N-glycanase (PNGase ...
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bioRxiv - Biochemistry 2023Quote: Cell pellet from panning experiment containing ∼106 cells was resuspended with 50 μL 0.1 mg/mL RNase A in 1x Phusion® HF reaction buffer (NEB, Cat. B0518S). Cells were vortexed at maximum speed for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then hybridized overnight at 37°C in hybridization buffer (2xSSC, 10% dextran sulfate [Sigma-Aldrich, D8906], 1mg/mL yeast tRNA [Sigma-Aldrich, R8759], 2mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Synthetic Biology 2024Quote: ... Assembly of the final CRISPRi plasmid was done by combining the libraries of the gRNA position vectors with the pST_301 plasmid in a Golde Gate reaction as described above but with Esp3I (NEB, 10,000 U/mL) instead of BsaI ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with the random colonies selected in LB agar plates with 50 µg/ml carbenicillin using OneTaq® 2X Master Mix (New England Biolabs, USA) and sequenced with Sanger method (Eurofins Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Purification of PCR products was performed with an in-house ExoSAP solution [50 μl of Exonuclease I (20,000 units/ml) (NEB® M0568, Ipswich, MA); 40 μl of Antarctic Phosphatase (5,000 units/ml) ...
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bioRxiv - Genetics 2022Quote: ... 2004) PCR reactions were performed in MiniAmp Plus Thermal Cycler with Q5® High-Fidelity DNA polymerase (2000 U/mL) (NEB Ipswich, Massachusetts) according to the manufacturer’s instructions ...