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Citations for New England Biolabs :
1551 - 1600 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Restriction digestion was carried out by adding 25 μL of 10 ×NEBuffer 2 and 100 U of the MluCI restriction enzyme (NEB, R0538) and incubating for ≥2 hours at 37°C in a Thermomixer at 900 rpm ...
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bioRxiv - Zoology 2021Quote: ... amplicons were analyzed by 1.5% agarose gel electrophoresis with ethidium bromide staining and using a DNA ladder marker (2 log, 100 bp, or 1 kb DNA ladder from New England Biolabs, USA). Expected PCR product sizes of the first step and nested PCR step were 514 and 148 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg of GST-beads were incubated for 15 min on ice in GRB buffer in a 25 μl reaction volume with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070) ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and either BICD2N-Halo (50 pmol) or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281; Chitin, New England BioLabs, S6651 ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequence was amplified from 50-100 ng of genomic DNA using Phusion High-Fidelity DNA Polymerase master mix PCR protocol (NEB #M0530). Gel purified PCR amplicons were sent to Integrated Genomic Operation (IGO ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments ranging from 3 kb to 5 kb with 40-100 bp terminal homologies were amplified from mouse BAC RP23-51O13 with Q5 polymerase (NEB, M0491L). Approximately equal amount (100 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng rRNA-depleted RNA from each nuclear fraction was used with the NEBNext Ultra II Directional library kit (NEB E7760S) to prepare RNA-seq libraries ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 2 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... nucleoplasm fractions (100 µg) were incubated with either mock (distilled water) or 50 Units of calf intestinal alkaline phosphatase (QuickCIP, NEB M0525L) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... nucleoplasm fractions (100 µg) were incubated with either distilled water (mock) or 50 Units of calf intestinal alkaline phosphatase (QuickCIP, NEB M0525L) and brought up to 100 uL with PBS ...
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bioRxiv - Genomics 2022Quote: ... we performed two separate 100 ul reactions (25 cycles each) with 250 ng of input gDNA using Q5 MASTER MIX (NEB #M0491) and the resulting products were pooled (PCR reaction ...
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bioRxiv - Molecular Biology 2019Quote: 300-500 ng purified mRNA was fragmented for 15 min at 70°C to ~100 nt using the NEBNext fragmentation module (NEB # E6150S) and purified using the Qiagen MinElute kit (Qiagen # 74204 ...
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bioRxiv - Developmental Biology 2019Quote: ... 100-200 ng total RNA was used to prepare an RNAseq library using an mRNA isolation kit (New England BioLabs, E7490) and a NEBNext Ultra RNA library prep kit (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... The digested samples were loaded on an agarose gel to detect and purify fragments ranging from 100-500 bps using the Monarch® DNA Gel Extraction Kit (New England Biolabs). The fragmented gDNA was incubated for 1 hr with 10 μM PhuS in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: Glands were dissected in cold PB+RVC (100 mM Na Phosphate Buffer pH 7.2, 10 mM Ribonucleoside-vanadyl complex; New England Biolabs, Ipswich, MA). During dissection ...
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bioRxiv - Cell Biology 2019Quote: ... 400 µL of lysate at the protein concentration of 1,5 µg/µL were added to 100 μL of washed Oligo d(T)25 Magnetic Beads (NEB, Ipswich, MA) and incubated at RT for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... eukaryotic recombinant Stlac2 was denatured with Glycoprotein denaturing buffer at 100 °C for 10 min to be deglycosylated using PNGase F (NEB, USA) according to instructions ...
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bioRxiv - Genetics 2020Quote: ... was annealed with 1ul of the reverse sgRNA (100 uM) using 1 ul 10X T4 ligation buffer (New England Biolabs, NEB), 1 ul T4 PNK (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... Purified embryo mRNA was fragmented to 100-200 nt RNA fragments by magnesium-buffer based degradation using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S). Fragmentation was optimized for time and RNA input amount monitoring RNA size using the mRNA Pico Chip (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 100 μM PvG748-SBS12-RT random hexamer primer (Supplementary Table 3) and 1 μl of 10 mM dNTP mix (NEB, N0447S), incubated for 3 minutes at 65 °C and transferred to ice ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng of total RNA was used to generate RNA-seq library using NEBNext Ultra RNA Library prep kit (NEB, E7530L) according to the vendor’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 100 µl volume) using primers described in Table S3C and Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Beverly, MA). This provides approximately 300X coverage for sequencing ...
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bioRxiv - Molecular Biology 2023Quote: Circular library preparation was performed in final T4 ligation mixture of 100 μl including 1X T4 DNA ligase buffer (NEB, USA), 1 μM phosphorylated library ...
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bioRxiv - Microbiology 2024Quote: ... 100 pmol oligonucleotide was incubated with 10 µCi [γ-32P]-ATP in the presence of 10 U T4 polynucleotide kinase (NEB) at 37°C for 2 hours ...
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bioRxiv - Plant Biology 2022Quote: ... Annealing of the previously designed forward and reverse sgRNA oligos (100 μM) was performed using the T4 Polynucleotide Kinase (NEB, UK). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were loaded on to 1% (w/v) agarose gels stained with Midori Green Advance DNA stain (Geneflow #S6-0022) alongside 100 bp ladder (NEB #N3231S), before submitting for DNA Sanger sequencing (Source Genomics ...
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bioRxiv - Biophysics 2023Quote: Tau was phosphorylated in vitro according to previous protocols with some modifications.12,88 50 µL of 100 µM tau was incubated with 2 µg cAMP-dependent Protein Kinase (PKA, New England BioLabs P6000S) and/or 0.4 µg GSK3β (Sigma-Aldrich G4296 ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 ng of RNA was reverse-transcribed into cDNA using the LunaScript RT Master Mix Kit (New England Biolabs #E3025L). qPCR reactions utilized specific primers and were performed in triplicate with Applied Biosystems TaqMan Gene Expression Master Mix (ThermoFisher #4369016) ...
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bioRxiv - Neuroscience 2024Quote: RNA-seq libraries were prepared from 100 ng RNA/sample using the NEBNext Ultra II RNA library preparation kit for Illumina (NEB E7770S) in conjunction with the NEBNext poly(A ...
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bioRxiv - Genomics 2024Quote: The gRNA libraries were amplified from each gDNA sample across 100 μL PCR reactions using Q5 hot start polymerase (NEB, M0493) with 1 μg of gDNA per reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... These products were then purified by separation on a 1.0% agarose gel at 100 volts constant for 1 hour and were then purified via the Monarch DNA gel extraction kit (NEB, #T1020L). Purified products were quantified with qubit high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: Restriction digestion was carried out by adding 25 μL of 10× CutSmart buffer and 100 U of the MluCI restriction enzyme (NEB #R0538) plus 100 U of the MboI enzyme (NEB #R0147) ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were washed in 2×SSC for 30 minutes for a total of four washes and then stored at 4°C in 2×SSC supplemented with 1:100 Murine RNase inhibitor (New England Biolabs, M0314S) for no longer than 2 weeks prior to imaging.
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bioRxiv - Bioengineering 2023Quote: ... and ligated with the digested pSubMAAP vector at a 100 ng : 37.5 ng vector : insert ratio using T4 DNA ligase (New England Biolabs, Ipswich, MA). The ligation reactions were desalted for 40 minutes using a membrane on molecular-grade H20 and electroporated into DH10B electrocompetent cells ...
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bioRxiv - Bioengineering 2023Quote: ... The components of the integration plasmids pTargetF_araA::P103/105/106/101/100-SIDF were sequentially assembled into pTargetF_araA GG using BsaI-HFv2 (NEB, Ipswich, MA, USA) digestion in four steps ...
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bioRxiv - Genomics 2023Quote: ... Hi-C library was purified by adding 80 μl Ampure beads and amplified in two 100 μl pre-amplification reactions (50 μl 2x NEBNEXT Ultra II Q5 Master Mix (NEB, M0544L), 5 μl 10 μM Nextera-P5-pre-Primer ...
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bioRxiv - Biochemistry 2023Quote: ... The total RNA was treated by DNase I (5 U per 100 μg total RNA) and mRNAs were enriched by NEBNext Poly(A) mRNA magnetic isolation module (NEB E7490L). 8-10 μg of mRNA from each sample was fragmented to the size of ~120-nt by Ambion Fragmentation reagent (Thermo Scientific AM8740 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml Tris buffer (10 mM Tris HCl (pH 8.0)) ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Immunology 2023Quote: ... The sequence flanking the SHP2 sgRNA target site was amplified by PCR from 100 ng of genomic DNA using Q5 DNA polymerase (NEB; M0491L) and the primers ...
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bioRxiv - Neuroscience 2023Quote: 2 ug of pX552 vector with SapI gRNA insertion site and Cre-dependent transgene was digested with 1:100 SapI enzyme (BioLabs #R0569S) in 1:10 CutSmart Buffer (BioLabs #B6004S ...
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bioRxiv - Neuroscience 2023Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS + 2% BSA + 0.2U/µl RNase inhibitor - New England Biolabs, Cat#: M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their shape ...
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bioRxiv - Neuroscience 2024Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS, 2% BSA, 0.2U/µl RNase inhibitor; New England Biolabs, cat# M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their morphology ...
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bioRxiv - Neuroscience 2024Quote: The MERSCOPE 500 gene imaging kit was activated by adding 250 μl of Imaging Buffer Activator (Vizgen, #203000015) and 100 μl of RNAse Inhibitor (New England BioLabs, M0314L). 15 ml of mineral oil was overlaid on top of the imaging buffer through the activation port ...
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bioRxiv - Developmental Biology 2020Quote: ... 3mM MgCl2) for 5 min before digesting with 0.2U/ml of DNase I (NEB). Embryos were then fixed in 4% PFA ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 mg/ml BSA and 0.04 unit/μl T7 DNA polymerase (New England Biolabs) at 37 °C for 3 hours ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria were immediately split into eight 1 mL aliquots of SOC (NEB, B9020S) and recovered for 1 h at 37 °C then independently expanded in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (EMD ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were incubated in 40 μg/ml PNGase F (New England Biolabs, MA) for 2 hr at 37 °C to cleave all N-glycans before immunoblotting 62 ...
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bioRxiv - Genomics 2022Quote: ... and then the reactions were treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...