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Citations for New England Biolabs :
1701 - 1750 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: The wells were then passivated by adding 40 ul of 20 mg/ml BSA (NEB) and incubated for at least one hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing Cdt1 were further purified on a 5 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.167 mg/mL MTSEA-biotin) for 30 min and purified with Streptavidin Magnetic Beads (NEB). The biotinylated RNA was eluted with 100 mM DTT and purified with RNA Clean beads (Vazymes) ...
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bioRxiv - Cell Biology 2023Quote: ... and M-MuLV reverse transcriptase enzyme (200,000 U mL−1, New England Biolabs, Ispwich, USA) was added per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL 20mg/ml BSA and 5 μL 400U/μl T4 DNA Ligase (NEB, M0202) overnight at 16°C (tumbling ...
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bioRxiv - Molecular Biology 2023Quote: ... a self-packed XK column (Cytiva) with 15 mL of Amylose resin (New England Biolabs) was attached to the HisTrap column ...
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bioRxiv - Immunology 2023Quote: ... The diluted supernatant was loaded onto a 5 mL amylose resin column (New England Biolabs). The column was washed with 150 mL of buffer I ...
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bioRxiv - Biochemistry 2023Quote: Infection in HCT116: Cells were infected with virus and 8 μg/mL polybrene (NEB, #H9268).
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 in the presence of 25 mU ml-1 apyrase (NEB, Cat. no. M0398S) and 1 µM of individual ligand ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Plugs were rinsed with TE and then washed with 1 ml NEB buffer 4 (NEB) 3 × 15min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of T4 DNA ligase high-concentration (2,000,000, units/mL, NEB, cat. no. M0202T) was added ...
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bioRxiv - Cell Biology 2023Quote: ... RNA recovered from sucrose density gradient fractions was treated with 600 units/ml heparinase (NEB P0735S ...
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bioRxiv - Biochemistry 2023Quote: ... Desalted peptides were dissolved in 1 ml 1 × CutSmart buffer (pH 8, New England BioLabs) and incubated with 50 units of alkaline phosphatase (calf intestinal ...
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bioRxiv - Molecular Biology 2024Quote: ... A self-packed XK column (Cytiva) with 15 mL of Amylose resin (New England Biolabs) was attached to the HisTrap column ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg/mL of both DNase I and RNase I (New England Biolabs, Ipswich, USA) was added and incubated with the phage at room temperature for 1 hour to digest any non-phage nucleic acid present in the mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 25% PEG-8000) into MilliQ and supplementing with 5.3 U/mL T5-exonuclease (NEB), 33.3 U/mL Phusion DNA polymerase (NEB) ...
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bioRxiv - Physiology 2024Quote: ... murine RNase inhibitor (500 U/mL, Cat. # M0314L; New England Biolabs, Frankfurt am Main, Germany) and heparin (10 mg/mL ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Genomics 2020Quote: ... 75 ng for 37PF and 100 ng for 362YU3) were fragmented using the NEBNext® Ultra™ II FS DNA kit (New England Biolabs, USA) at 37°C for 20 minutes (resulting in DNA fragments with an average size of 350 bp) ...
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bioRxiv - Genomics 2020Quote: ... RNAseq was performed with 100 bp paired-end reads using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Pooled DNA (100 ng) was fragmented and barcoded using the NEBNext→ Fast DNA Fragmentation and Library Prep Set for Ion Torrent™ (NEB Inc, UK) and IonXpress Barcode Adapters kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... coli DNA and 2 μg of Salmonella DNA in a total of 100 μL each were added to the NEBNext® Ultra™ II End Repair/dA-Tailing module (New England Biolabs (NEB), USA ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were run on a 1% agarose gel stained with ethidium bromide alongside a 100 bp ladder (New England Biolabs, Ipswitch, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons were gel purified and 100 ng of the mutated linearized plasmids were re-ligated by using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, Ipswich, MA, USA), following the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared from 100 ng of DNA using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB E7805L) with NEBNext® Multiplex Oligos for Illumina® (E7600S) ...
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bioRxiv - Cancer Biology 2024Quote: ... and chromatin was digested overnight by adding 25 µL 10X NEBuffer 2 and 100 U Mbo I (New England Biolabs, catalog No. R0147). Digestion efficiency was confirmed by isolating DNA from a 25-µL aliquot of the suspension and performing agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2023Quote: ... The inserted barcoding sequences were annealed using randomized overlapping single stranded oligonucleotides by adding 3 µl of each oligonucleotide (100 µM) to 500 µl 1x 2.1 NEB-buffer (New England Biolabs, Ipswich, MA, USA, #B6002S) followed by boiling at 100 °C for 3 minutes to finally let them associate during the cooling to room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was prepared by combining 2 µL of 100 µM guide RNA (gRNA) with 2 µL of 20 µM EnGen SpyCas9 NLS (NEB, Cat. No. M0646T), and incubated at room temperature for 2-4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... the HeLa cells were kept in HeLa culture media treated overnight with 0.2 U/mL of □2-3,6,8,9 Neuraminidase (New England Biolabs) to cleave sialic acid from the cell surface glycans.
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bioRxiv - Molecular Biology 2021Quote: 2.5 mg of DNA were resuspended in 20 mL of 1X CutSmart Buffer (NEB cat#B7204S) and incubated at RT for one hour on a rotating wheel.
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bioRxiv - Evolutionary Biology 2021Quote: ... with either Proteinase K at 200 μg/mL final concentration (New England Biolabs, Ipswich, MA, USA) to assess for any protein-dependent effect or sonication using a Branson 450 Digital Sonifier (3 pulses of 15 sec ...
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bioRxiv - Genomics 2022Quote: ... The electroporated bacteria was immediately split into ten 1 mL aliquots of outgrowth medium (NEB, B9035S) and incubated at 37°C for 1 hr then independently scaled up in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (Teknova ...
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Bioengineering 2019Quote: ... Aliquots of individual sputum samples were pretreated with 20 U/mL of DNase I (NEB, MA) for subsequent fluorometric assays of mucin and DNA concentrations ...
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bioRxiv - Biochemistry 2019Quote: ... To obtain the nucleotide free (apo) state 50 U/mL of alkaline phosphatase (New England Biolabs) was used ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... cells were treated with sialidase (200 U/mL Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase, New England BioLabs) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was collected and mixed with 1 ml of amylose agarose resin (New England Biolabs) pre-equilibrated with buffer A per 50 ml of cell homogenate ...
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bioRxiv - Microbiology 2022Quote: ... Filtered (0.45 μm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Microbiology 2021Quote: ... immediately rescued in 1 ml of 37°C SOC outgrowth medium (New England Biolabs, cat#B9020S) and incubated with shaking at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... 6μg of sonicated DNA was pretreated with 12 μl of RNase H (5000 U/mL; NEB) for incubation at 37°C for 6h prior to immunoprecipitation by S9.6 antibody ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µl T4 DNA ligase buffer and 2 µl 10 mg/ml BSA (New England Biolabs). These reactions were placed in a thermocycler with following program ...
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bioRxiv - Neuroscience 2022Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Microbiology 2019Quote: ... cells were suspended in 1 ml of super optimal broth with catabolite repression (S.O.C. medium) (NEB) and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Thawed cells were resuspended in 5 ml of 1X NEB2 restriction enzyme buffer (New England Biolabs) and poured into a pre-chilled mortar containing liquid N2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 M guanidine HCl) containing 8 units/ml and Proteinase K (P8107S, New England Biolabs Inc.) was added freshly to the digestion buffer ...
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Immunology 2019Quote: ... CytoFIX was pre-incubated on ice for 2min with 40IU/mL murine RNAse inhibitors (M0314, NEB). After 15min of incubation at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was then equilibrated twice in 1 ml of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...