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Citations for New England Biolabs :
1751 - 1800 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 4°C) and the supernatant was added to 24 mL of amylose resin (NEB, Ipswich MA) pre-equilibrated with buffer C and nutated for 2 hr at 4°C ...
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bioRxiv - Genetics 2019Quote: ... Thawed cells were resuspended in 5 ml of 1X NEB2 restriction enzyme buffer (New England Biolabs) and poured into a pre-chilled mortar containing liquid N2 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Desialylation of ACE2-wt-Fc was performed with 2500 U mL-1 neuraminidase (New England Biolabs) in 50 mM sodium citrate (pH 5.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then 0.3 μL of 1 mg mL−1 trypsin (trypsin-ultra, MS-grade, New England Biolabs) was added and samples were incubated at 37 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Sonicated salmon sperm DNA was then diluted to 1 mg/ml in Terminal Transferase buffer (NEB) with 0.25 mM CoCl2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive control slides were treated with 3 U/mL DNase-1 (New England Biolabs, Cat. M0303) for 10 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Tn5 transposase was bound by mixing the cleared lysate with 30 mL of chitin resin (NEB) in a column and rotating end-to-end for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... each chamber is filled for 30 min with a 1 mg/ml solution of Streptavidin (NEB) and then passivated with a blocking solution (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... incubating mixture with concentrated (2,000,000 units/mL) T4 DNA ligase and ligase buffer (New England Biolabs) at 16°C for 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... The restriction enzymes used were either BsaI or BsmBI (both 10 000 U/mL from NEB).
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bioRxiv - Genomics 2022Quote: ... PIP particles were suspended in buffer with 29 U/mL Proteinase K Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Genomics 2024Quote: ... and pooled into 7 ml ligation buffer (1X ligation buffer 3 (New England Biolabs; without ATP), 1 mM ATP ...
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bioRxiv - Microbiology 2024Quote: ... 5 units (2.5 μL) of RNase free DNAse I (NEW ENGLAND BioLabs, 2000 u. mL-1) and 100 μL of nuclease free water was added for every 10 μg of RNA ...
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bioRxiv - Pathology 2023Quote: ... Filtrates were treated with a cocktail of 2.0 μL of micrococcal nuclease and 1.0 μL benzonase (2,000,000 gel units/mL Micrococcal nuclease, NEB Inc ...
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bioRxiv - Microbiology 2023Quote: ... Filtered (0.45 µm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Systems Biology 2023Quote: ... 400 µL of RNAlater and 2 µL of 20 mg/mL BSA (New England Biolabs # B9000S) were added to the samples and incubated on ice for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... 40 U/mL Rnasin) and then resuspended in ligation buffer plus 2 μM SplintR Ligase (NEB). Cells were incubated for 1h at 37 ºC with gentle agitation.
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10 in PBS-BSA (PBS supplemented with 0.4 mg/mL BSA (New England Biolabs)) ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were subsequently treated with 10 U of calf intestinal alkaline phosphatase (NEB, 10,000 U/ml) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... 17 μl total RNA (100 ng/μl) was added with 2 μl buffer and 0.5 μl RNase-free DNaseI enzyme (New England Biolabs GmbH, Frankfurt am Main, Germany) incubating on ice for 30 s ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2019Quote: Metagenomic libraries were prepared from DNA (100 ng) using the NEBNext® Ultra II FS DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) > 100ng input protocol as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: Purified guide crRNA and template RNA was obtained by following this in vitro transcription as a 100 µL batch reaction with a DNA digestion step (0.035 U/µL DNaseI, 1x DNaseI Buffer, NEB No. M0303S, 37°C, 1h) and a phenol-chloroform extraction protocol in a 5’-Phase Lock Gel Heavy (VWR ...
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bioRxiv - Genomics 2020Quote: PCR products were loaded into a single lane on a 1% agarose gel with 100 bp DNA ladder (New England Biolabs, Frankfurt am Main, Germany). Fragments between 200 and 500 bp were cut from the gel with a scalpel and purified using the QIAquick gel extraction kit (QIAgen ...
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bioRxiv - Genomics 2020Quote: ... Between 100 and 200 ng of genomic DNA was digested using 2 units of the MslI restriction enzyme (New England Biolabs ®, Ipswich, Massachusetts, USA.) and using 1 unit of NEB4 buffer in 20 μl of volume and for 2 hours at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: The RNA-seq libraries were prepared from 100 μg of total RNA per genotype with NEBNext Ultra II RNA Library Prep Kit mRNA Isolation Module (NEB, Cat# E7775S, E7490S, E7500S). Quality control and sequencing of the RNA-seq libraries were performed by GENEWIZ ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 100 ng of DNA was cleaved with PstI HF and MseI restrictases in CutSmart® buffer (New England Biolabs; 3 hours at 37°C). P1 and P2 barcoded adapters corresponding to the restriction site of the enzymes were ligated immediately afterwards with T4 ligase (NEB ...
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using a pre-poured amylose column containing 4 mL amylose resin (New England Biolabs, E8021L) followed by size exclusion chromatography (protein buffer ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... GST-GGA3 was grown overnight in 50 mL of Laria-Bertani broth in NiCo21 E Coli (NEB). The following day the overnight culture was transferred to 1L of terrific buffer ...
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bioRxiv - Bioengineering 2019Quote: ... the lysate was digested by ∼0.2 mg/ml Proteinase K (New England Biolabs, Cat. # P8102 or P8107S) at 55°C for ∼1 day ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The tissue sections were then lysed in lysis buffer and Protinease K (10mg/ml, New England BioLabs). The lysis buffer contained 50 mM KCL ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: PCR was performed on toe clippings that were incubated overnight at 55° C in tail lysis buffer (10mM Tris pH 8, 0.4M NaCl, 2mM EDTA, 0.1% SDS, 3.6U/mL Proteinase K (NEB)) ...
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bioRxiv - Biophysics 2021Quote: ... and 3 samples from each replicate combined into 7 mL with 1 × T4 DNA Ligase Buffer (NEB), with 1% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was restricted with 66.7 U/ml AluI (R0137, New England Biolabs, Ipswich, MA, USA) in CutSmart buffer (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was incubated with gentle shaking for 1 h with 10 mL of amylose resin (NEB) pre-equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... The cell suspension was then mixed with 5 ml ligation buffer (550 μl 10x ligation buffer (NEB), 27.5 μl 20 mg/ml BSA (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... Gelated samples were digested in 4 U/ml proteinase K buffer (New England Biolabs, Ipswitch, MA, USA) with 50 mM Tris pH 8.0 (Serva ...
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bioRxiv - Biochemistry 2022Quote: ... mixing with 5 μL of stop buffer (50 mM EDTA and 2 mg/ml Proteinase K (NEB)) ...
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bioRxiv - Biochemistry 2019Quote: ... and concentrated to 2 mg/mL and incubated with 3125 Units of Endoglycosidase H (Endo H)(NEB) per milligram of protein for 3 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was dripped twice through a 10 ml packed volume of amylose resin (New England Biolabs), washed with 5 column volumes of column buffer (20 mM Tris ...
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bioRxiv - Genomics 2020Quote: ... washed with ChIP buffer then resuspended in 1.6 mL of of MNase buffer with 1x BSA (NEB). The nuclei solution was preincubated at 37°C for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... and resuspended in 1 x DPBS with 0.4 mg/ml bovine serum albumin (# 74719, New England Biolabs) at approximately 1000 cells/µl ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were drained of all liquids before 2 mg/ml of Proteinase K (New England Biolabs) diluted in PK buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... IP and input samples were incubated with 10 μL of 800 U/mL Proteinase K (NEB P8107S) at 37° for 2 hours ...
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bioRxiv - Genetics 2020Quote: ... 10 mL of a 10% BSA solution was incubated with 2.5 μL of Proteinase K (NEB P8107S) for 30 minutes at room temperature (∼22°C) ...
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Molecular Biology 2024Quote: The flow cell chamber was passivated with 30 µL PBS-BSA buffer (1.0 mg/mL BSA (NEB) in PBS ...