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Citations for New England Biolabs :
1601 - 1650 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 1 mM calcium chloride and 200 unit/ml final concentrations of micrococcal nuclease (NEB). Cell lysates were incubated at 25 ℃ for 15 minutes before adding 3 mM final concentration of EGTA was added.
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bioRxiv - Developmental Biology 2020Quote: ... Reaction was stopped by depleting ATP with 4.5 U/ml apyrase (New England Biolabs) for 10 min at room temperature ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The remaining sample was diluted in 10 ml 1x T4 DNA ligase buffer (NEB) containing 1% Triton X- 100 and incubated at 370C and 750 rpm for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were quenched with 2% H2O2 and digested in 10µg/ml Proteinase K (BioLabs). The reaction was stopped with 2mg/ml Glycine (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... cultured HEK cells expressing Endoglycan were treated with O-glycosidase (8’000 U/ml; NEB) or α2-3,6,8 Neuraminidase (5 U/ml ...
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bioRxiv - Biochemistry 2021Quote: ... After slowly cooling down T4 DNA ligase (final concentration of 40 U/mL, NEB) was added and the reaction was performed for 2 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The template was destroyed by adding 1 μL 20,000 U/mL DpnI (NEB, R0176S) to the mix and incubating at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin reverse crosslinking was performed by adding 50µl of 20mg/ml proteinase K (NEB) and 120µl of 10% SDS and incubated at 55C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and 7U/mL Klenow Fragment of DNA Polymerase I (3’-5 exo-) (NEB, M0212). Reactions were incubated at 37°C and incorporation of labeled dCMP was monitored by an acid precipitation assay ...
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bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Bovine serum albumin at final 0.2 mg/mL and Phi29 polymerase (New England BioLabs) were added and the reaction ran at 30°C for 16 hours before inactivation at 65°C for 10 minutes.
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bioRxiv - Microbiology 2022Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, M0242, 200,000 units/ml) and incubated at 16°C for 14 h ...
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bioRxiv - Developmental Biology 2020Quote: ... the wings were treated with 1.25 µl (20 mg/ml) proteinase-K (NEB, P8107S) in 1 ml 1X PBST and then with 2 mg/ml glycine in 1X PBST ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was degraded by adding 50 µl of 20mg/ml proteinase K (NEB, P8102), 50 µl of 10% SDS and incubated at 55°C for 30 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... and the supernatant was adsorbed to a 0.4 ml amylose resin (New England Biolabs) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 0.45 mL of pre-equilibrated oligo(dT)25 magnetic beads (New England Biolabs, #S1419S) were added to the lysates and incubated for 1 h at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.02% NP-40) including 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was collected and passed through a 2 mL Amylose resin (NEB E8021S) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... The soluble fraction was applied to 10 ml of chitin beads (New England Biolabs) equilibrated with chilled buffer A ...
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bioRxiv - Molecular Biology 2020Quote: ... The chambers of the microplate were pre-treated with 1 mg/ml BSA (NEB) for 30 min before aspirating the BSA ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant fraction was incubated with 4 ml amylose resin (New England Biolabs, E8021L) for 1 h ...
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bioRxiv - Biophysics 2020Quote: ... The flow cell was incubated for 10 minutes with 1 mg/ml streptavidin (NEB) and then 10 minutes with Blockaid (ThermoFisher) ...
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bioRxiv - Genomics 2022Quote: ... PIP particles were suspended in buffer with 29 U/mL Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNase I (2 µL, 2,000 U/ml, RNase-free, New England Biolabs, Ipswich, MA), 10× DNase buffer ...
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bioRxiv - Genomics 2022Quote: ... we spike in 2.5 µL of 3M U/mL T7 DNA ligase (NEB M0318L) and continue incubating for 1 hour at 25C ...
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bioRxiv - Neuroscience 2023Quote: ... 20µL sample + 6µL H2O + 1µL proteinase K (200µg/mL) (New England BioLabs, Ipswich, MA). 3 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were treated with 200 U/mL of α2,3,6,8,9 neuraminidase A (NEB Bioke, P0722L) and 0.6 U of α1,2,3,4,6-L-fucosidase (Megazyme ...
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bioRxiv - Neuroscience 2022Quote: ... and nuclei were resuspended in 2 mL 1x NEBuffer 3.1 (New England Biolabs; B7203S) + 20uL NxGen RNase Inhibitor.
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bioRxiv - Microbiology 2022Quote: ... 87.5 µl of Proteinase K (800 units/ml) (New England Biolabs, Ipswich, MA, USA) were added ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions were further purified on 7.5-10 mL amylose columns (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.13 mM S-adenosylmethionine) and 50uL of 4,000U/mL M.CviPI GpC methyltransferase (NEB, M0227L). Each methylation reaction was then incubated for at 37 °C shaking at 1000 rpm for 7.5 minutes (unless specified otherwise ...
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bioRxiv - Genetics 2024Quote: ... 120 μL (4 mg/mL) streptavidin magnetic beads (NEB cat no. 50-812-660) were washed 3 times with 1 mL of lysis buffer then resuspended in 100 μL of complete lysis buffer and added to the hybridization mix and then incubated at 37 °C for an additional 30 min with mixing ...
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bioRxiv - Biophysics 2023Quote: ... 0.05% (v/v) Tween 20 (SigmaAldrich) and 40 mg/mL BSA (New England Biolabs).
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bioRxiv - Biophysics 2023Quote: ... Lysates were applied to 10-mL bed volume of chitin resin (New England BioLabs) that was pre-equilibrated with column buffer for 1.5 hours at 4°C on a tube rotator ...
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bioRxiv - Biophysics 2023Quote: ... 0.05% (v/v) Tween 20 (SigmaAldrich) and 40 mg/mL BSA (New England Biolabs), pH 7.9 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted sample was incubated with 2 ml amylose resin (New England Biolabs, E8021L) for 1 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 250 μL of sample with 16 μg/mL TPCK-treated trypsin (New England BioLabs), 2X penicillin and streptomycin and 2X antibiotic-antimycotic (Wisent ...
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bioRxiv - Microbiology 2023Quote: ... Extracellular nucleic acids were removed with DNase I (New England Biolabs, 20 U/mL) and RNase A (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then cooled and treated with 50 μg/ml proteinase K (PK, NEB) for 18 h at 60°C ...
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bioRxiv - Biochemistry 2024Quote: ... in the presence of 5mM CaCl2 and 25mU/mL apyrase (NEB, Cat. no: M0398S), for 2h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... All samples were pelleted and resuspended in 8μg/mL recombinant albumin (New England Biolabs) in dPBS[-/-] for encapsulation with the Chromium X instrument (10X Genomics) ...
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bioRxiv - Immunology 2024Quote: ... 0.4 µl of 2000 U/ml Phusion® High-Fidelity DNA Polymerase (NEB, #M0530S), 2 µl of the universal forward primer (5’-AAGCAGTGGTATCAACGCAGAG-3’ ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and placed in single-cell suspension in 1 mL RPMI+10% FBS ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and stained with LIVE/DEAD Aqua for 15 minutes at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... ∼500 ng of genomic DNA was digested for 3 hours in a 50 μl reaction with 100 units each of the restriction enzymes EcoRI-HF and Msp1 (New England Biolabs, Ipswich, MA), and 1X CutSmart Buffer (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... Assembly was performed with 1650 ng of BsmBI-digested pLentiCRISPRv2 and 250 ng of amplicon in a total reaction volume of 100 μL with HiFI DNA Assembly Mix (NEB, Ipswich MA) for 30 min at 50°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was then treated with DNase for 10 min at 37 °C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Neuroscience 2020Quote: ... Methylation standards (100 % and 0 %) were either prepared by in vitro methylation of human genomic DNA using M.SssI CpG methyltransferase (NEB, Frankfurt a.M; Germany) or by whole-genome amplification using the REPLI-g Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: The rAAV:HDR:cleaved plasmids were linearized with BspQI restriction enzyme and spacer sequences with compatible overhangs were ligated into 100 ng of plasmid backbone using ElectroLigase® (NEB M0369) following the manufacturer’s protocol ...