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Citations for New England Biolabs :
1651 - 1700 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281; Chitin, New England BioLabs, S6651; or Amylose, New England BioLabs, E8021) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cell lysates were adjusted to the same protein concentration using furin cleavage buffer (100 mM Tris-HCL and 1mM CaCl2) and incubated with 5 units of Furin (NEB, Ipswich, MA) for 16 h at 30℃ ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was carried out on 100 ng of gDNA per sample using Phusion High-Fidelity DNA Polymerase (New England Biolabs, Hitchin, UK) with an initial denaturation phase (98°C for 30 seconds) ...
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bioRxiv - Genomics 2020Quote: ... The RNA-Seq library was prepared with 100 ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, PN E7490S) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (PN E7760S) ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibodies were diluted in the same Triton X 100 and BSA solution and suppliers and source were: primary 1:200 anti-CK20 rabbit D9Z1Z (New England Biolabs, Herts, UK), anti-CD31 mouse JC70/A (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared from 100 ng of input RNA using the non-directional kit NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, #E7775) with the NEBNext Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... coli DNA and 2 μg of Salmonella DNA in a total of 100 μL each were added to the NEBNext® Ultra™ II End Repair/dA-Tailing module (New England Biolabs (NEB), USA ...
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bioRxiv - Genetics 2020Quote: ... 100 ng of DNA isolated from peripheral blood was digested with the methylation-sensitive HpaII enzyme (New England Biolabs, Ipswich, MA, USA), as originally described (39) ...
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bioRxiv - Zoology 2021Quote: ... The size of DNA fragments were estimated by visual inspection using a 100 bp DNA ladder marker (New England Biolabs. Inc, UK)
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bioRxiv - Biochemistry 2020Quote: ... Excess iodoacetamide was quenched with DTT and the samples were digested overnight with endoproteinase GluC (1:100) at room temperature (New England Biolabs, Product #P8100S). Digested peptides were dried in a vacuum concentrator ...
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bioRxiv - Microbiology 2020Quote: ... RNA (25-100 ng) was mixed with random oligonucleotide primers using the NEBNext® First Strand Synthesis Module kit for Illumina® (NEB) and incubated for 10 min at 94°C ...
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bioRxiv - Biophysics 2021Quote: ... was annealed with primer P3 (100 bp long) at a 1:3 DNA to primer molar ratio and ligated using T4 DNA ligase (NEB, catalog #M0202S). Primer P3 had biotin modification at its 3’ end ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples run under reducing and deglycosylating conditions were reduced by treatment of the samples with dithiothreitol (final concentration, 100 mM) and deglycosylated with PNGase F (New England Biolabs, Ipswich, USA), respectively following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... A 25 μg aliquot of CP-treated CTDNA was diluted to 100 μL of 1X nucleoside digestion mix reaction buffer (NEB, Ipswich, MA) at pH 5.4 and treated with 2.5 μL NEB nucleoside digestion mix (1 μL or 1 U per 10 μg CTDNA ...
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bioRxiv - Genetics 2022Quote: ... cDNA was produced from 100 ng of total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) with the d(T)23 VN primer ...
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bioRxiv - Microbiology 2022Quote: DNA was isolated from 100 µL of whole blood using the blood protocol from the Monarch® Genomic DNA Purification Kit (New England Biolabs, Australia). In addition ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA was then treated with DNase for 10 min at 37◦C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or four (white shavenbaby experimental) sgRNA targeting the first or second exon (100 ng/μl) and CAS9 protein (EnGen Spy Cas9 NLS from NEB, Catalog # M0646M). The distribution of the major trichome height for the white control was normal (Shapiro-Wilk normality test W = 0.9637 ...
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bioRxiv - Genetics 2023Quote: ... Ab aliquot of DNA (100 ng) was used to create libraries using the NEBNext Ultra II kit (New England Biolabs, Ipswich, Massachusetts). Libraries were sequenced at The University of Texas at Austin Genome Sequencing and Analysis Facility on a HiSeq 4000 ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... and then the PCR reactions were treated with 200 U/ml of Exonuclease I (NEB) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was treated with 200 mg/ml of DNAse I (New England BioLabs) for 10 min at 37°C followed by 10 min at room temperature and then clarified by centrifugation at 1500 g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... H2O2-treated lysed cells were incubated with 0.8 units/mL Fpg enzyme (New England Biolabs) in Fpg reaction buffer (40mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was applied onto a column with 1 ml of equilibrated amylose resin (NEB). The column with the beads and the extract was incubated on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 1× protease inhibitor cocktail) and incubated with 12,000 gel units/ml micrococcal nuclease (NEB, M0247S) at 37 °C with frequently mixing to digest chromatin to lengths of approximately 100-to-1,000 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 μL of 10 mg/mL Proteinase K (New England Biolabs, Ipswich, MA, Cat # P8107S) and 2.5 μL of 10 mg/mL RNase A (Thermo-Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μL of molecular biology grade bovine serum albumin (20 mg/mL, New England Biolabs), 5 μL of 5× Phire Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: Viral stocks were pretreated with Dnase I (10U/ml, New England Biolabs Inc, MA, USA) and Benzonase nuclease (50U/ml ...
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bioRxiv - Biochemistry 2022Quote: ... and the PCR reactions were then treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of highly concentrated HhaI enzyme (150,000 U/mL, NEB; Cat. Num. R0139B-HC1), while keeping the remaining reagents as stated in the Tapestri protocol (5 μl Forward Primer Pool ...
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bioRxiv - Molecular Biology 2022Quote: Chromatin de-crosslinking was performed by adding 50 µl of 20mg/ml proteinase K (NEB) and 120 µl of 10% SDS and incubated at 55°C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of 10 mg/mL BSA and 50 μL of T4 Ligase (NEB M0202L) in a total volume of 1000 μL with nuclease-free water ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... 0.0625 µL T7 DNA ligase (Enzymatics),0.0625µL of 20 mg/mL Bovine Serum Albumin (NEB), 2x Reaction Ligation Buffer (Enzymatics ...
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bioRxiv - Genomics 2019Quote: ... the isolated DNA was reverse-crosslinked (adding 10 mg/ml proteinase K (NEB, Ipswich, America) and 1% SDS (AMBION ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% SDS) + 10 μL of 10 mg/mL Proteinase K (New England Biolabs, Cat # P8107S). DIP digestion buffer was added to input samples up to 200 μL ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Microbiology 2021Quote: ... 0.7 μL of 20 mg mL-1 bovine serum albumin (New England Biolabs, MA, USA), 0.75 μL of each primer (10 μM) ...
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bioRxiv - Bioengineering 2022Quote: ... 200 Ω) and cells were recovered in 1 mL SOC medium (New England Biolabs, #B9020) at 30 °C and for 90 min ...
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bioRxiv - Molecular Biology 2023Quote: ... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.125 μl of Shrimp Alkaline Phosphatase (1 U/ml; New England Biolabs, cat. no. M0371S), 0.0125 μl Exonuclease I (20 U/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage samples were pre-treated with 2.5 μL of DNase I (2,000 units/mL, NEB), 2.5 mM MgCl2 and 0.5 mM CaCl2 in 200 μL of each diluted concentration of phage samples at 37 °C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: The flow cell chamber was passivated with PBS-BSA buffer (1.0 mg/mL BSA; NEB) in PBS for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The pellets were resuspended in 1.25 mL of NEB quick lysis buffer (NEB, Ipswich, USA) supplemented with 0.5 mg/mL lysozyme ...
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bioRxiv - Bioengineering 2023Quote: ... Frozen cell pellets were resuspended in 4 mL of IMAC buffer (NEB, Ipswich, MA, USA) on ice and dispersed using an ultrasonic disruptor (Sonics ...