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Citations for New England Biolabs :
1801 - 1850 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 2 µL of 2000 U/mL DNase I to the IVT reaction mixture (New England Biolabs). Template DNA digestion was carried out at 37 °C for 30 minutes in a thermocycler block prior to purification of the RNA using a Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting eluant was further treated with 1% SDS and 8 U/mL proteinase K (NEB, P8107S) at 42°C for 20 minutes then phenol chloroform extracted and ethanol precipitated.
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bioRxiv - Biophysics 2023Quote: ... The clarified and filtered lysate was loaded on 1 mL of IgG Sepharose fast flow resin (NEB). The column was washed with lysis buffer and TEV cleavage buffer (lysis buffer with 75 mM NaCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... nucleic acids were digested by addition of 10 µL of DNaseI (2000 units/mL, New England Biolabs) and CaCl2 to a final concentration of 10 mM ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of each phage was treated with 1 µl DNase (New England Biolabs, USA) at 37 ⁰C for 40 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction mixture for polymerization contained 25 units/ml Hot-start Taq DNA polymerase (New England Biolabs), Taq standard reaction buffer ...
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bioRxiv - Biophysics 2024Quote: ... The resulting annealed structure was then combined with 1 µL of DraIII-HF (NEB; 100,000 units/mL) and 1 µL of BaeGI-HF (NEB ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nuclei were pelleted at 500 ξ g at 4°C for 5 minutes and resuspended in 0.5 mL of 1.2x NEBuffer r2.1 (New England Biolabs) containing 3% sodium dodecyl sulfate (SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μL of 100 μM the forward and reverse DNA oligos were annealed in 47 μL annealing buffer (5 μl NEB buffer 3.1 and 42 μL H2O) by 5 min incubation at 95 °C and slow cool down in the heating block overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in 20 µl reactions and products were run on a 1% agarose gel alongside an appropriately sized ladder (either New England Biolabs 100 bp or 1kb DNA ladder) and stained post-electrophoresis with GelRed® (Biotium) ...
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bioRxiv - Microbiology 2020Quote: ... the inserts and the plasmids were mixed with Gibson master mix 2x (100μL 5X ISO Buffer, 0.2 μL 10,000 U/mL T5 exonuclease (NEB #M0363S), 6.25 μL 2,000 U/mL Phusion HF polymerase (NEB #M0530S) ...
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bioRxiv - Molecular Biology 2021Quote: ... their nuclei were isolated via centrifugation then resuspended in a 0.5 ml solution comprising 1.2 × restriction enzyme NEBuffer™ r3.1 (NEB) containing 0.3% SDS ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysis was cleared by centrifugation at 16,100g for 10 min and 0.65 ml supernatant was mixed with 50μl Protein G beads (NEB, #37478S) pre-conjugated to U1-70K antibody (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This was then loaded and incubated in a gravity column containing 25 mL of chitin bead slurry (NEB), then washed with 400 mL column buffer (20 mM TrisHCl pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was then further purified by a second affinity step over an 8 mL amylose column (NEB), where it was washed with 3 CV of Amylose Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... in 57 ml using end repair buffer from NEBNext Ultra II DNA library preparation kit for Illumina (NEB). Glucosylated DNA was then end repaired without purification followed by ligation to Pyrrolo-dC adaptors as indicated in NEBNext Ultra II DNA library preparation protocol (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... The template was eliminated after the PCR reactions by addition of 1 µL DpnI (10,000 U/mL, NEB) to 25 µL PCR reactions and incubation at 37 °C for at least 1 h ...
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bioRxiv - Microbiology 2021Quote: Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... 10 µL of Gibson Master Mix 2X(100μL 5X ISO Buffer, 0.2 μL 10,000 U/mL T5 exonuclease (NEB #M0363S), 6.25 μL 2,000 U/mL Phusion HF polymerase (NEB #M0530S) ...
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bioRxiv - Physiology 2022Quote: ... Diluted lysate was then incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The chromosomal DNA was digested with 2.25 µl of BglII restriction enzyme (New England Biolabs; 50000 U/ml) for 2 h and 30 min at 37°C followed by the addition of 0.25 µl of BglII and incubation for another 30 min at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... the fluidic cell was incubated with 10 mg/ml bovine serum albumin (BSA, New England Biolabs, Ipswich, USA) to prevent non-specific surface binding ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM EDTA, 200 mM NaCl, 0.2% SDS, supplemented with 0.2 mg/ml Proteinase K from New England Biolabs) at 55°C overnight ...
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bioRxiv - Bioengineering 2019Quote: ... at 15 units/mL was injected over the flow cell 1 in Neb2.1 buffer 1x (New England BioLabs) with a flow rate of 5 μL/min for 15 min at 12 °C ...
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bioRxiv - Microbiology 2020Quote: ... the inserts and the plasmids were PCR amplified and then mixed with Gibson master mix 2x (100µL 5X ISO Buffer, 0.2 µL 10,000 U/mL T5 exonuclease (NEB #M0363S), 6.25 µL 2,000 U/mL Phusion HF polymerase (NEB #M0530S) ...
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bioRxiv - Cell Biology 2020Quote: ... Paired barcode and splints were phosphorylated in a PCR plate by combining 20 μl of oligonucleotides with 40 μl phosphorylation mix (1X T4 ligation buffer, 0.2 mg/ml BSA, 0.167 U/μl T4 PNK (NEB)) and incubated for 30 min at 37°C and heat inactivated for 20 min at 65°C ...
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bioRxiv - Bioengineering 2021Quote: ... 1:15 and 1:30 (PEI-AuNCs:siRNA)) were incubated with RNase H (10μg/ml, New England BioLabs, M0297L) at 37°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Ligation was performed on a 2 mL reaction scale with 1X T7 DNA Ligase buffer (New England Biolabs), 4 µM dsDNA adaptor with two different 4 base sticky-ends ...
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bioRxiv - Genomics 2021Quote: ... so nuclei were lysed directly in plates by aspirating all liquid and then adding 1.8 mL of 0.3% SDS in 1x NEB2 buffer (New England Biolabs) per 10 cm dish ...
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bioRxiv - Biochemistry 2021Quote: ... 400 nM each of phosphorylated forward and reverse primers and 0.25 µL of Phusion Polymerase (2,000 U/mL) (NEB). PCR products were run on 1% agarose gels to confirm successful amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... and crosslinks were reversed with 10% SDS and 0.6 mg/ml Proteinase K (New England Biolabs, Ipswich, MA) overnight with shaking at 65 °C ...
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bioRxiv - Genomics 2022Quote: ... 13.1 μL Murine RNAse inhibitor, 5.25 μL Tartrazine (10 mg/mL, Carl Roth) and 13.2 μL T4 DNA ligase (New England Biolabs). 4 μL of the master mix were added to the Ligation Barcoding Plate #1 or #2 (see above ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were washed three times for 5’ each with PBS-0.01%Tween and incubated with 0.24 mg/ml Monarch RNase A (New England Biolabs) for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.6 mM of A, C, and T, NEB-N0446S; 0.1 µM Clean.G; 800 U/ml Bst Polymerase, NEB-M0374L ...
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bioRxiv - Microbiology 2023Quote: ... Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Genetics 2023Quote: ... following which they were incubated in blocking buffer composed of 0.5 mg/ml BSA (New England Biolabs, #B9001S), 80 units of RNAse-OUT RNAse inhibitor (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... treating each well with 100 μl of lysis buffer (0.45% NP40, 0.45% Tween20, 0.5x NEB Q5 PCR buffer, 0.1 mg/mL NEB Proteinase K) were incubated for 10 min at 55°C in and subsequently heated to 95°C for 10 min to inactivate the proteinase K ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently mixed with ∼0.2 ng of coilable ∼3.3 kbp dsRNA and 1 mg/ml bovine serum albumin (BSA, New England Biolabs). The ∼3.3 kbp length includes only the stem ...
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bioRxiv - Genomics 2023Quote: ... Approximately 7,500 cells were diluted with 1.25 ml of a dilution buffer containing 0.4x NEBuffer 2 (NEB, B7002S), 2 mg/ml BSA (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly reactions were performed in a volume of 10 µL with 0.5 µL of either Esp3I (10,000 U/mL, NEB) or BsaI (10,000 U/mL Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were resuspended in Rnase H reaction buffer containing 20 U/mL of Rnase H enzyme (NEB M0297S). Cells were incubated for one hour at 37 °C with gentle agitation ...
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bioRxiv - Genetics 2024Quote: ... An additional 20-minute digestion was then performed with 5 µL RNase A (NEB #T3018, 20 mg/ml). The rest of the protocol remained unchanged ...
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bioRxiv - Neuroscience 2024Quote: After washed with 0.1 3 SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L), tissue sections placed on the chip were permeabilized using 0.1% pepsin (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... coli CJ236 was picked into 1 ml of 2YT media (Roth, 6676.2) supplemented with M13KO7 helper phage (NEB, N0315) to a final concentration of 1 × 108 pfu/ml and ampicillin (final concentration 100 µg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... To remove the adaptor sequences from the resulting PCR products up to 2.5 μg of product was transferred to a new 1.5 ml tube and was digested with AlwI restriction enzyme (New England Biolabs) at 37 °C for 16 hours ...
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bioRxiv - Genetics 2021Quote: Cells from each fin sample obtained as described before were washed in 2 ml PBS supplemented with 0.1% bovine serum albumin (BSA) (NEB). Each sample was divided into two replicate tubes ...
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bioRxiv - Cell Biology 2022Quote: ... mixed with 10 μl of lysis buffer (10 mM Tris-HCl [pH 8.8], 1 mM EDTA, 25 mM NaCl, 1% SDS and 8U/ml Proteinase K, NEB) for 3 min at room temperature ...