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Citations for New England Biolabs :
1851 - 1900 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... and resuspended in 50 µL of 1 mg/mL RNAse A solution (New England Biolabs, Ipswich, Massachusetts, United States) and statically incubated at 37 °C for 3 h ...
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bioRxiv - Genomics 2020Quote: ... 500 ng of intact gDNA was digested overnight with 2.5 μl of MspI (20,000 units/ml; NEB cat. R0106S) in the manufacturer’s supplied buffer (40 μl total volume ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1.5 mg of protein was cleaved in a 2 ml reaction with 240 Units of TEV protease (NEB) for two hours at 30 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs] ...
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bioRxiv - Synthetic Biology 2021Quote: ... carrying all remaining components in a Golden Gate reaction performed as described above but with Esp3I (10,000 U/mL, NEB) instead of BsaI ...
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bioRxiv - Systems Biology 2020Quote: ... We discarded the supernatant and resuspended the pellet in 2 mL of 1x NEB buffer 3.1 (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was passed three times over a column of 10 ml of amylose resin (New England Biolabs, E8031L). The column was washed with MBP wash buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... before incubation during 6 min in 50μl of PNK reaction mix (1x PNKT, 1 mM ATP and 0.05 U/ml T4 PNK 3′phosphatase minus (NEB) in a thermomixer at 37°C and 1400rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... Half of each forebrain was immersed in 4.8 ml nuclei extraction buffer (NEB [Sucrose 0.32M, CaCl 5mM, Mg(Ac).4H20 3mM ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were performed using Q5© high-fidelity DNA polymerase [2,000 units/ml] (New England Biolabs, Germany) in triplicates ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was dissolved and digested in 50 ml buffer A with 2 mM CaCl2 and 4,000 units of micrococcal nuclease (M0247S, NEB) at RT for 20 min ...
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bioRxiv - Biophysics 2022Quote: ... The coupling reaction was performed at 25 °C for 1 h and followed by addition of 0.2 unit/mL apyrase (New England Biolabs). After additional 1 h at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The RNA was first hydrolyzed overnight to nucleotide monophosphates using 300 U/ μg Nuclease P1 (NEB, 100,000 U/mL) at 37°C in 100 mM ammonium acetate (pH 5.5 ...
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bioRxiv - Genetics 2022Quote: ... The swab was then given to the primary investigator and placed in a 1.5 ml microcentrifuge tube containing 400 μl of Cell Lysis Buffer from the Monarch Genomic DNA Purification Kit (NEB). Samples were stored in Cell Lysis Buffer at 4°C until processed.
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bioRxiv - Immunology 2022Quote: ... T cell clones were thawed and allowed to rest at 37°C in RPMI containing DNAse (5µg/mL, New England Biolabs).
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bioRxiv - Evolutionary Biology 2022Quote: ... the pre-cleared lysates were collected and mixed with the biotin labeled pre-miRNA or biotin labeled shuffled pre-miRNA as negative control in the final concentration of 8.23 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Microbiology 2021Quote: ... Then homogenized lysate incubated with 0.80 mL of pre- equilibrated oligo(dT)25 magnetic beads (New England Biolabs, #S1419S) for 1 h at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2020Quote: ... Infection was done with HIV-1NL-GFP passed through 0.2 μM filters (MilliporeSigma Durapore) and treated with 20 U/mL DNaseI (New England Biolabs) for 60 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA was isolated from single fins by placing them in lysis buffer (10mM Tris pH 8, 100mM NaCl, 10 mM EDTA, 0.5% SDS) with Proteinase K (333µg/mL, NEB P8107S) at 55°C for between four hours and overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were then washed once in 5 volumes of binding buffer (100μL, 1xPBS containing 1mg/mL of BSA (NEB)) for 10 minutes at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Diatom exconjugants were selected on 1/2L1 1% agar medium supplemented with 20 μg mL−1 phleomycin (Gold Biolabs) at a diel cycle of 14:10 at ~50 μE ms−1 light intensity.
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bioRxiv - Genomics 2019Quote: ... the DNA was treated with 2 μml of NEBNext FFPE DNA Repair Mix and 2 μml of NEBNext Ultra II End-prep enzyme mix in a 60 μml volume that also included μml of FFPE and 3.5 μml of End-prep reaction buffers (New England Biolabs). The reaction was performed at 20°C for 5 min and 65°C for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl) and incubated for 10 min at 37 °C with 2.000 units/mL of DNase I (New England BioLabs). Half of the resulting sample was incubated with 10 µg/mL of PK (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... the IVT template RNA (0.01– 0.05 A260 units) was digested into nucleosides using 10 µg/ml nuclease P1 (New England Biolabs, M0660S), and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was diluted with one volume of water and treated with 0.5 μL of 20 mg/mL proteinase K (New England Biolabs) at 50°C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... All buffers used in the nuclei isolation and sorting contained 0.4 U/mL RNAse inhibitor murine (New England Biolabs). Cross-linked samples were incubated with 800 μL quenching buffer (1 M Tris-HCl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR reactions contained 10 ng of template DNA and 1X 5PRIME HotMasterMix (Quanta Biosciences) as well as 0.4 mg ml-1 BSA (NEB) and 0.4 μM of each primer ...
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bioRxiv - Biochemistry 2021Quote: ... nuclei from 4 x 107 of cells were resuspended in 0.5 ml of MNase digestion buffer and digested with 2400 U of MNase (NEB) in the presence of 3.3 mM CaCl2 and 0.2% Triton X-100 at 30°C for 12 min with vigorous shaking (1200 rpm) ...
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bioRxiv - Biochemistry 2021Quote: ... Heparinase digests were performed for 2 hours at 37 °C with a mixture of Heparinase I + II and III (3.5 mUnits/ml, NEB) in Heparinase digestion buffer (20 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed with 1xPBS and blocked for 30 minutes at 37°C in blocking buffer (1xPBS, 0.05% BSA [Invitrogen, Am2616], 50μg/mL yeast tRNA [Sigma-Aldrich, R8759], 10mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Biochemistry 2021Quote: Recombinant SARS-CoV-2 S protein constructs HexaFurin and HexaPro were concentrated to 0.5 mg/mL in Assay Buffer and incubated with the indicated concentrations of furin protease (NEB) or dasTMPRSS2 ...
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bioRxiv - Microbiology 2021Quote: ... 4°C and the supernatant applied on a 6 ml gravity flow column with Chitin resin (New England Biolabs), equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were treated with 25 µg Proteinase K (1.25 µL of 20 mg/mL stock solution, New England Biolabs) and incubated at 50 °C for 20 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... by gradient salt dialysis in the presence of 0.2 mg/ml nuclease-free bovine serum albumin (BSA, New England Biolabs). Quality of reconstitution was assessed by MNase and chromatosome stop assays (Figure 2-figure supplement 1D&E).
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bioRxiv - Neuroscience 2024Quote: ... and then washed with 0.1x SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L) (NEB, M0314L). RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42°C using SuperScript II (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... incubated at 37°C for 5 minutes, and then washed with 0.1x SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L) (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... The filtered lysate was passed over a gravity-flow column packed with 0.5 ml amylose resin (New England Biolabs) that had been pre-equilibrated with extraction buffer ...
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bioRxiv - Systems Biology 2024Quote: ... the cDNA was diluted to 0.05 µg/ml and PCR-amplified using Phusion DNA polymerase (New England Biolabs M0530) with 0.5 mM dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... Plugs were then equilibrated twice for 30 min at room temperature in 1 ml of 1x NEBuffer 3.1 (New England Biolabs). After the buffer was completely discarded ...
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bioRxiv - Microbiology 2023Quote: ... Enzymes BsaI-HF®v2 (#R3733S) and BsmBI-v2 (#R0739S) (10.000 U/mL) and T7 ligase (#M0318S) were obtained from New England Biolabs (NEB). PCR amplicons were first cloned into the pYTK001 entry vector before assembly into a composite construct using preassembled vector backbones with GFP dropouts ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL of RNase H (5,000 U/mL) (New England Biolabs, Fig. 2f and Extended Data Fig. 2c,d) were treated with 1.5 of 10× RNase H buffer in the sample by adjusting with Milli-Q H2O to total 15 µL ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the annealed oligos were phosphorylated in a 10 µl reaction containing 0.25 µl T4 PNK (10000 units/ml, NEB) and 1 µl 10x T4 ligase buffer for 40 min at 37°Ϲ before heat-inactivation at 65°Ϲ for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... 4.1 μl of PLPPR3 ICD (final concentration 0.075 mg/ml) was mixed with 0.2 μl purified PKA catalytic subunit (final concentration 20 000 Units; #P6000S, Biolabs), 0.8 μl phosphorylation buffer (final concentration of 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Snap-cooled RNAs were diluted to 8 nM in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
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bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Biochemistry 2024Quote: ... Cleavage reactions were incubated at 37°C for 1 hour and subsequently worked up with 0.2 mg/mL RNase A (New England Biolabs) and 4 units of Proteinase K (New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... Then the suspension was incubated at room temperature for 1.5 h after adding apyrase (25 mU/mL; New England Biolabs). The supernatant was removed by ultracentrifugation at 40,000 rpm for 30 min ...