Labshake search
Citations for New England Biolabs :
1701 - 1750 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using Taq (New England Biolabs) or Phusion (Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: PCR was performed using OneTaq polymerase (NEB #M0480). Reactions consisted of 1X OneTaq Standard Reaction Buffer ...
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bioRxiv - Biophysics 2023Quote: ... PCR was performed using Q5 DNA Polymerase (NEB) followed by purification using DNA clean and concentrator spin-column purification (Zymo) ...
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bioRxiv - Cell Biology 2023Quote: ... using Luna PCR Master Mix (New England Biolabs). Fold induction was calculated by comparing relative gene expression to the housekeeping gene 18S RNA using the ΔΔCT method64.
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bioRxiv - Immunology 2023Quote: ... and PCR product was digested with DpnI (NEB) and in some cases ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the PCR products were digested (NEB restriction enzymes) and cloned directionally into plasmid pBIN61 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were obtained using Q5 Polymerase (NEB) or Expand Long Template PCR system (Roche/Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: The Q5 high-fidelity PCR polymerase (NEB #M0491) was used to amplify the fragment tail fiber fragment E0 (gene 17 1146-1662 ...
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bioRxiv - Systems Biology 2024Quote: ... After PCR amplification by Phusion DNA polymerase (NEB) and TBS380 (Picogreen ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: ... PCRs were conducted using Q5 DNA polymerase (NEB) for cerN (primers 2850 & 2851) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was digested with DpnI (NEB) for 2 h at 37 °C and transformed into chemically competent DH5α Escherichia coli.
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bioRxiv - Neuroscience 2023Quote: ... PCR fragments were amplified using Q5 polymerase (NEB). Both vectors and PCR fragments were purified using gel electrophoresis and gel extraction (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and performed colony PCRs with Taq polymerase (NEB). We used the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... high-fidelity PCR reactions (NEB Q5 DNA Polymerase) with pBSV2G and pcon++flgV were performed using primers PA470 + PA467 and PA466 + PA467 ...
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bioRxiv - Plant Biology 2024Quote: ... PCR using Q5 High-Fidelity DNA polymerase (NEB), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions with Q5 high fidelity polymerase (NEB) were carried out as follows ...
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bioRxiv - Genetics 2023Quote: ... PCR amplified with Q5 polymerase (New England Biolabs) and incubated with restriction enzyme-digested CD510-B1 plasmid (SystemBio ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was DpnI (New England Biolabs) digested at 37 °C for 2 h without prior clean-ups ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were digested with Hpy166II (R0616S, NEB).
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bioRxiv - Evolutionary Biology 2024Quote: ... amplified by PCR with Phusion DNA polymerase (NEB) with RBNS index primers and RBNS reverse primer I (see below) ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were amplified using -inverse PCR (Q5, NEB), using primers with overhangs containing spacer sequences ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was incubated with EcoRV (NEB) that cuts inside mCherry for 30 min at 37°C to linearize any remaining template DNA ...
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bioRxiv - Microbiology 2024Quote: Colony PCR was performed using either Q5 (NEB) or Sapphire master mix (Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR reaction was DpnI (New England Biolabs) digested (37°C ...
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bioRxiv - Microbiology 2022Quote: ... Five microliters of the mixture was added to Luna Universal Probe One-Step RT- qPCR mixture (NEB, MA, USA) to a final volume of 20 µl ...
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bioRxiv - Genomics 2020Quote: ... were combined with RT primer mix [1 μl 250 ng/μl randomhexRT primer and 0.5 μl 10 mM dNTPs (NEB)] ...
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bioRxiv - Molecular Biology 2019Quote: ... SI) were incubated for 2 hours at RT in T4 ligase buffer with 400 U of T4 ligase (NEB) in a total volume of 100 µl ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl was added to a 9 µl Luna® Universal One-Step RT-qPCR reaction (NEB, Cat# E3005L). RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 0.5-1 µg of RNA per sample using Protoscript II RT and Random Primer Mix (New England Biolabs). qPCR reactions were performed on cDNA using iQ SYBR Green supermix (BioRad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... modified and CE treated IVT RNA were ligated to ONT RT Adapter using concentrated T4 DNA ligase (NEB-M0202T), and was reverse transcribed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific-18080044) ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reaction was then amplified using PHUSION® high-fidelity DNA polymerase using HF buffer (New England Biolabs) via PCR to generate a 1675-bp amplicon ...
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bioRxiv - Microbiology 2023Quote: ... The thermal cycle of the RT-qPCR machine was set up based on NEB #M3003 instruction manual (NEB, UK). Transcription values (Ct ...
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bioRxiv - Microbiology 2023Quote: ... The thermal cycle of the RT-qPCR machine was set up based on NEB #M3003 instruction manual (NEB, UK). Transcription values (Ct ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed in CutSmart buffer, and incubated (1h, RT) in 150 µL blunting mix (112.5 µL ddH2O, 15 µL 10x blunting buffer, NEB, 15 µL 100 µM dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted from the gel overnight in 400 µl of 0.4 M NaCl with rotation at RT and precipitated with 96% ethanol in the presence of linear acrylamide (NEB).
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bioRxiv - Pathology 2023Quote: ... Each 10 µL reaction contained Luna Probe One-Step RT-qPCR 4X Mix (New England Biolabs, Ipswich, MA, USA), primers (10 µM) ...
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bioRxiv - Microbiology 2023Quote: ... Glycans were removed by incubating the immunoadhesins ON at room temperature (RT) with endoglycosidase H (endo-H, 0.8 U/mL, #P0702L, New England Biolabs) in 50 mM Na acetate-50 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using 10-fold-diluted cDNA and the Luna Universal qPCR Master Mix (New England Biolabs) in the CFX Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... The nuclei were washed two times with a plastid-lysis buffer (NEB + 2% triton) followed with centrifugation (5 min at 1500 g ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of plasmid DNA was used per PCR reaction and used in a volume of 50 µl using Next High-Fidelity 2x PCR Master Mix (NEB, M0541) (according to the manufacturer’s protocol) ...
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bioRxiv - Genomics 2020Quote: Primer-walk PCRs were performed with edited single-cell clones to identify aberrant PCR products with OneTaq 2x Master Mix (NEB M0486L) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and pre-amplified by PCR in the presence of adapter primers (NEBNext 2x PCR Master Mix, New England Biolabs Inc. #M0541S). Amplification was monitored by RT-PCR using the PowerUp™ SYBR™ Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... After that tagmended library (20 µL) was PCR amplified using 25 µL NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541L), 2.5 µL of P5_BRB primer (5 µM ...
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bioRxiv - Synthetic Biology 2020Quote: ... Reporter plasmids were assembled by Gibson Assembly using promoter PCR product and pHCKan-yibDp-GFPuv PCR product following manufacturer’s protocol (New England Biolabs, Ipswhich, MA). All plasmid sequences were confirmed by DNA sequencing (Genewiz ...
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bioRxiv - Genetics 2021Quote: ... barcodes and barcoding adapters (PCR Barcoding Expansion 1-96, EXP-PBC096, Oxford Nanopore Technologies, UK) by PCR using Q5 polymerase mastermix (NEB, USA) for individual fish identification according to manufacturer’s protocol in a 25μl reaction (98°C for 3 min ...
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bioRxiv - Genomics 2021Quote: ... Purified DNA was subjected to an initial step of PCR amplification consisting of 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) and standard barcoded primers of Nextera kit for each sample ...
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bioRxiv - Plant Biology 2021Quote: ... A 2.3 kb amplicon including the NAC18.1 gene and ∼800 bp of upstream sequence was amplified by PCR using primers NAC18F2 and NAC18R2 (Table S1) and Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB). PCR product size and purity was confirmed by agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymo clean Gel DNA Recovery Kit (Zymo Research,D4002) ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were generated by PCR (primers 341F and 806R) using a Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Amplification product quality was assessed by gel electrophoreses and samples were pooled in equimolar ratios ...
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bioRxiv - Developmental Biology 2022Quote: ... TE buffer (20 µL) was added and PCR was performed using NEBNext High-Fidelity 2X PCR Master mix (New England Biolabs, M0541) as follows ...