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Citations for New England Biolabs :
1951 - 2000 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 200 ng of total RNA were ligated to the pre-annealed custom RT adaptors (21) using concentrated T4 DNA Ligase (NEB-M0202T). Ligated RNA was reverse transcribed using Maxima H Minus RT (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... Included in the gel as controls were an RT reaction conducted in the absence of tRNA and a Small Range RNA Ladder (NEB N0364S). The gel was stained with SYBR gold and visualized using a ChemiDoc imager (BioRad) ...
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bioRxiv - Systems Biology 2024Quote: ... 3 µl of 100 µM barcode-linked oligo-dT primer was added to each well with 5 µl of 5x ProtoScript RT buffer (New England Biolabs M0368). The plate was incubated at 94°C for 2 min and immediately cooled on ice for at least 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 250ng of polyA(+) RNA were ligated to pre-annealed custom RT adaptors (IDT) containing barcodes 78 with T4 DNA concentrated Ligase (NEB-M0202M) for 15 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... 250 ng of total RNA was used to synthesize cDNA using LunaScript®RT SuperMix (New England BioLabs Inc, Massachusetts, USA). Quantitative PCR amplification was performed using PerfeCta SYBR Green PCR master mix (QuantaBio ...
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bioRxiv - Biochemistry 2023Quote: ... Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies ...
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bioRxiv - Genomics 2023Quote: ... 800 ng of poly(A)-tailed RNA (200 ng per curlcake construct and 250 ng per riboswitch) was ligated to ONT RT Adaptor (RTA) using concentrated T4 DNA Ligase (NEB, M0202T). The optional reverse transcription step was performed using SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... an optional PBS wash followed by Red Blood Cell lysis step was included (10 mins at RT) prior to hypotonic lysis with Nuclei Prep Buffer (NEB, Cat.T3052) and Rnase digestion ...
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Cell Biology 2022Quote: ... The V3-V4 hypervariable regions of bacterial 16S rRNA genes were amplified by PCR with Phusion® High-Fidelity PCR MasterMix (New England Biolabs, Ipswich, MA, USA) using specific primer as previously described59 ...
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bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... Almost full-length PCR products were obtained in a preliminary PCR using 1.25U OneTaq® Quick-Load® DNA Polymerase (New England BioLabs, Ipswich, MA, USA), 200 μM mixed dNTPs ...
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bioRxiv - Microbiology 2024Quote: ... 5’-GCT GCG TTC TTC ATC GAT GC-3’) were chosen with barcodes for PCR amplification using Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA). The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were cloned into pMiniT (New England Biolabs), and the stop codon was altered to create a NheI site using site-directed mutagenesis ...
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bioRxiv - Synthetic Biology 2021Quote: ... All plasmid-template PCR products were treated with DpnI (NEB) following standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplified (8-12 cycles) with Phusion polymerase (NEB) using custom primers (22) ...
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bioRxiv - Genetics 2020Quote: ... We performed this PCR using Taq DNA Polymerase (M0320S, NEB) and the following conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (NEB). Each reaction contained 1x Q5 reaction buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1X Phusion High Fidelity PCR Mastermix (New England Biolabs). Cycle conditions were 98°C for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA polymerase (NEB) while qPCR products were amplified using Ssofast EvaGreen Supermix (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified PCR fragment was subcloned via HIFI cloning (NEB) under a Math1 enhancer.
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bioRxiv - Molecular Biology 2020Quote: ... PCR was carried out with Phusion polymerase (New England Biolabs), with PCR cycling conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified PCR product was digested with Hpy188I (New England Biolabs) for 2 hours at 37 °C to generate single dT 3’ overhangs ...
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bioRxiv - Genomics 2019Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2020Quote: ... PCR mix (NEBNext Ultra II Q5 Master mix, NEB M0544), USER enzyme (NEB M5505) ...
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bioRxiv - Genomics 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed with Q5 polymerase (New England Biolabs) in the buffer provided ...
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bioRxiv - Cell Biology 2019Quote: ... PCR reactions were performed using Phusion polymerase (New England Biolabs). PCR products were digested with DpnI restriction enzyme (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... PCR was performed for using Q5 DNA polymerase (NEB #M0491S) with 1X GC buffer (72°C 5min ...
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bioRxiv - Genomics 2019Quote: ... PCR was performed for using Q5 DNA polymerase (NEB #M0491S) with 1X GC buffer (72°C 5min ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was conducted using Q5® High Fidelity DNAPolymerase (NEB). DNA fragments were separated in 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... digesting the PCR product with BamHI-HF (New England Biolabs), dephosphorylating with Quick CIP (New England Biolabs) ...
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bioRxiv - Immunology 2019Quote: ... The PCR product was purified and assembled into BsmBI (NEB) digested vector backbones in a Gibson reaction (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed using Phusion high HF buffer (NEB) and standard PCR conditions (98°C for 30s ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed using Phusion high HF buffer (NEB) and standard PCR conditions (98°C for 30s ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed using Q5 polymerase (New England Biolabs Inc) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Correct PCR products were digested with DpnI (New England Biolabs) to remove the template and subsequently cleaned up with a DNA cleanup kit (Zymo Research ...
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bioRxiv - Microbiology 2019Quote: PCR was performed using Phusion HF polymerase (New England Biolabs). All restriction enzymes and T4 polynucleotide kinase were purchased from New England Biolabs and used according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... PCR was performed for using Q5 DNA polymerase (NEB #M0491S) with 1 × GC buffer (72°C 5 minutes ...
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bioRxiv - Physiology 2021Quote: ... PCR was done using Q5 High-Fidelity DNA polymerase (NEB), PCR primers as listed in Table 1 ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added to each well and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA absence was confirmed with PCR using Taq polymerase (NEB) with primer 1 & 2.
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR (qPCR) with the Luna Sybr-Green mastermix (NEB) used primer sequences in Table S3 and 2 μL of a 1:10 dilution of the cDNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed with Phusion polymerase (New England Biolabs) and TruSeq primers (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... and they were obtained through PCR with Q5 Polymerase (NEB). The PCR products were subjected to 1% agarose gel electrophoresis and they were purified using a Zymogen gel DNA recovery kit (Zymo Research) ...
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bioRxiv - Neuroscience 2021Quote: ... the PCR product was digested with the AflII enzyme (NEB) whose recognition site is at the targeted cut-off site ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (NEB). For reactions using Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Cell Biology 2021Quote: ... were cloned using overlap PCR followed by Gibson assembly (NEB) cloning into pHAGE-C-HA/FLAG and were resistant to siRNA # 0 ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was cloned into pMini T2.0 (NEB).