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Citations for New England Biolabs :
1901 - 1950 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The PCR was completed using 1x reaction buffer (NEB), 200 µM dNTPs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... indexing PCR was performed using Q5 (New England Biolabs) under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... New vectors were generated by PCR (Phusion-HF, NEB) into parent vectors digested with FseI/AscI unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... PCRs were performed using Phusion polymerase (New England Biolabs). Details on primer sequences ...
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bioRxiv - Biophysics 2023Quote: ... PCR products were digested with BamHI and NotI (NEB) and ligated into BamHI/NotI-digested pACYC-Duet-1 vector (Novagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed with Taq polymerase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A Q5 high fidelity PCR reaction (New England Biolabs) was performed on the cDNA to amplify TsKSRP and TsAGO genes using primers encoding a c-myc tag at the 3’ end ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: ... All PCRs were conducted using Q5 DNA polymerase (NEB). Primer sequences are listed in the supplemental data (DataSet1 ...
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bioRxiv - Cell Biology 2023Quote: ... All PCRs were made using Q5 DNA polymerase (NEB). All constructs were sequence verified ...
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bioRxiv - Neuroscience 2023Quote: ... End point PCR analysis (Taq 2X Master Mix, NEB) was performed using 300 ng DNA template and 2-LTR circle primers ...
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bioRxiv - Microbiology 2023Quote: ... Each gene was PCR-amplified (Phusion; New England Biolabs) along with ∼300 bases 5’ of the translation start site to include the native promoter using primers designed with 16- 20 bases complementary to the gene of interest and 18-22 bases that overlap the NcoI site in pMV306hyg (Table S4) ...
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bioRxiv - Neuroscience 2023Quote: ... were amplified by PCR employing Q5 polymerase (NEB#M0494). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... 25μl NEBNext High-Fidelity 2x PCR Master Mix (NEB), 2.5μl Nextera XT V2 i5 primer and 2.5μl Nextera XT V2 i7 primer (Illumina) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and PCR-amplified 2.5 mM DNTPs (NEB, CAT#N0447S), 10X PCR buffer (Klentaq ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR products were treated with DpnI (NEB) to remove the template plasmid DNA and then self-ligated using Ligation High Ver2 (Takara) ...
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bioRxiv - Genomics 2024Quote: ... and NEBNext High-Fidelity 2x PCR Master Mix (NEB) for 12 cycles to generate each library ...
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bioRxiv - Bioengineering 2024Quote: ... primarily using PCRs (Q5 ultra II HotStart Polymerase, NEB), Gibson Assemblies (NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were circularized via ligation (New England Biolabs). For Melt-effector fusions ...
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bioRxiv - Synthetic Biology 2024Quote: ... After 5 PCR cycles using Q5 polymerase (NEB #M0491) with 5X GC Buffer with a melting temperature of 72°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The oligo was obtained from IDT DNA and amplified by PCR using Phusion Polymerase (NEB F630) and purified for in vitro transcription using the MEGAshortscript T7 kit (Invitrogen AM1333) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was digested by EagI (New England Biolabs) and ligated into FMR1 RNA construct backbone instead of 5’UTR of FMR1 sequence using T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... we amplified the targeted gene regions by PCR (NEB Next High-Fidelity 2xPCR Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were performed using the Q5 DNA polymerase (NEB) in a GeneAmp PCR system 2400 (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were subsequently ligated using T4 ligase (NEB) in a reaction with DpnI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting PCR product was treated with DpnI (NEB), gel purified using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... A colony PCR using Taq Polymerase (New England Biolabs) on positive resistant colonies can help identifying false positives for Ampicillin resistance ...
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bioRxiv - Bioengineering 2024Quote: ... Genotyping PCR was then conducted using Q5 polymerase (NEB) for genomic DNA and cDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was cloned with BamHI (R0136S, NEB), NheI (R3131S ...
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bioRxiv - Cancer Biology 2021Quote: ... agarose plugs containing DNA were washed three times with 1X NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Microbiology 2019Quote: ... and the product library was cleaned 3 times with XP AMPure Beads (New England BioLabs) to remove short fragments and primers (bead ratios 1X and 0.6X ...
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bioRxiv - Plant Biology 2023Quote: ... Ligation was performed by increasing the volume 5 times with T4 DNA ligase buffer (NEB) to a final concentration of 1X ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, MA, USA) and high-fidelity polymerase (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with forward primer “reverse transcription primer (RTP)” ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with cDNA diluted 1:10 in water ...
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bioRxiv - Genomics 2023Quote: ... Amplification of the libraries was performed for 13 PCR cycles using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, cat. no. M0531L); 6-bp molecular barcodes were also incorporated during this PCR amplification ...
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bioRxiv - Systems Biology 2023Quote: ... PCR with custom Nextera PCR primers was then performed for five cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Ipswich, MA, USA) 76 ...
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bioRxiv - Microbiology 2023Quote: ... Amplification was achieved through 16S rRNA gene Polymerase Chain Reaction (PCR) with Illumina (San Diego, USA) adapted primers [50] and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, USA) at 98°C (2 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted genome DNA was used to amplify the surrounding region of the m.15059G>A mutation for T7 Endonuclease I (T7EI) mismatch detection assay using Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, USA) in accordance with recent studies 27 ...
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bioRxiv - Molecular Biology 2020Quote: ... 800 nanograms of Poly(A)-tailed and capped RNA – 200 ng per construct – was ligated to ONT RT Adaptor (RTA) using concentrated T4 DNA Ligase (NEB-M0202T), and was reverse transcribed using SuperScript III RT (Thermo Fisher Scientific-18080044) ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 ng total of polyA tailed yeast RNAs were ligated to pre-annealed custom RT adaptors (IDT) (Smith et al., 2019) using concentrated T4 DNA Ligase (NEB-M0202T), and was reverse transcribed using Maxima H Minus RT (Thermo Scientific ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Reverse transcription of RNA to cDNA was performed by combining 500 ng/ mL of the RNA samples with the LunaScript™ RT SuperMix (5X) (NEB) in 20 μL aliquots ...
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bioRxiv - Molecular Biology 2019Quote: ... 800 nanograms of Poly(A)-tailed and capped RNA — 200 nanograms per construct-was ligated to ONT RT Adaptor (RTA) using concentrated T4 DNA Ligase (NEB-M0202T), and was reverse transcribed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific-18080044) ...
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bioRxiv - Molecular Biology 2021Quote: ... were heated to 65°C and slow-cooled to 37°C before reverse transcription with 5 U AMV-RT (NEB, M0277L) at 42°C for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 100 μM PvG748-SBS12-RT random hexamer primer (Supplementary Table 3) and 1 μl of 10 mM dNTP mix (NEB, N0447S), incubated for 3 minutes at 65 °C and transferred to ice ...
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bioRxiv - Genomics 2021Quote: ... The sample was then allowed to cool to room temperature and incorporated in the Klenow enzyme mix (1x Maxima h-RT buffer, 1mM dNTP, 1U/μL of Klenow Exo-; NEB M0212L) was added to the single strand library ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted 1:25 in water and used as template for RT-qPCR using the Luna Universal qPCR master mix (NEB M3003S). Primers used are listed in Supplementary Table 3 ...
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bioRxiv - Genomics 2022Quote: ... 500 ng poly-A RNA or poly-A tailed IVT RNA was ligated to the ONT RT adaptor (RTA) using T4 DNA Ligase (NEB, M0202M). Then the product is reverse transcribed using SuperScript™ III Reverse transcriptase (Invitrogen ...
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bioRxiv - Pathology 2023Quote: ... each custom RT adapter was ligated to 500 ng of rRNA-depleted and polyA-tailed RNA samples using T4 DNA Ligase (NEB M0202L), which was followed by reverse transcription using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) ...