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Citations for New England Biolabs :
1501 - 1550 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... PCR was performed using Phusion HF (NEB) and TruPE PCR primers (Illumina) ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed with Q5 polymerase (NEB) following manufacturer’s instructions with 10 PCR cycles (primers listed in Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... PCR with Q5 High Fidelity Polymerase (NEB) was used to generate insert and backbone DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Phusion HF PCR Master Mix (1x, BioLabs), water (6.42 µL) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR product was digested with DPNI (NEB), followed by treatment with T4 Polynucleotide kinase (PNK ...
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bioRxiv - Developmental Biology 2022Quote: ... and a second round of PCR (NEB Q5 polymerase ...
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bioRxiv - Molecular Biology 2022Quote: ... mTurbo was amplified by pfusion PCR (NEB) using Ti833/Ti834 as primers and 3xHA-miniTurboNLS_pCDNA3 (addgene #107172 ...
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bioRxiv - Genetics 2023Quote: ... All libraries were PCR amplified (NEB M0544S) for 5 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed using Phusion polymerase (NEB). Unique Fluidigm sample barcodes were added using Fast Start High Fidelity PCR System (Roche) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products and NdeI/XhoI (NEB, USA) digested pAN001 vector DNA were assembled using Gibson assembly standard protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Q5 PCR Master Mix (New England BioLabs) was used for PCR reactions ...
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bioRxiv - Cell Biology 2023Quote: ... 14-cycle PCR with index primers (NEB) was performed (initial denaturation ...
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bioRxiv - Biochemistry 2023Quote: ... PCR was performed using Phusion polymerase (NEB) and primers that add Illumina adapter sequences and a 6 bp identifier sequence used to distinguish cell populations ...
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bioRxiv - Bioengineering 2023Quote: ... PCR with Q5 polymerase from NEB (M0493S), followed by HiFi assembly (NEB E2621S ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were digested by ExoI (NEB) to remove excess of oligos and purified using Qiagen PCR purification kit ...
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bioRxiv - Genomics 2024Quote: ... 20uL 2X NEBnext PCR master mix (NEB) was added ...
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bioRxiv - Microbiology 2024Quote: ... PCR was conducted using Q5 polymerase (NEB) following manufacturer’s guidelines ...
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bioRxiv - Biophysics 2022Quote: Site-specific mutagenesis was performed with the Q5® Site-Directed Mutagenesis Kit (based on PCR-amplification) according to the manufacturer’s protocol (New England Biolabs GmbH, Frankfurt am Main, Germany). Oligonucleotides were ordered from metabion GmbH (Planegg/Steinkirchen ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit, New England Biolabs, GmbH, Frankfurt am Main, Germany). PCRs were performed using the Q5 polymerase (Q5 Hot Start High-Fidelity ...
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bioRxiv - Plant Biology 2023Quote: ... The constructs M1 and M2 were used as templates to generate higher-order mutants by site-directed mutagenesis PCR (NEB, Q5 Site-Directed Mutagenesis Kit) using the primers listed in Table S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The CXCL8 promoter was amplified by PCR with Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB) according to the manufacturer’s instruction with the following primers:
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bioRxiv - Genomics 2020Quote: ... Transposed DNA was PCR amplified using the NEB Next High-Fidelity PCR Master Mix (New England Biolabs, Hitchin, UK) and primer Ad1_noMX in combination with one of six barcoded primers (Table 1 ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA libraries were made by a limited cycle PCR Using the NEBNext High-fidelity 2X PCR Master Mix (NEB). To maximize the complexity of DNA libraries ...
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bioRxiv - Microbiology 2019Quote: Index PCR was performed with 25µL NEBNext Hig-Fidelity 2X PCR master mix (New England Biolabs Inc., Ipswich, MA), containing 5µL of each index primer (Nextera XT Index kit v2 set A ...
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bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a final concentration of 50mM and 12.2µl of each tagmented sample was PCR amplified for 15 cycles using 2x NEB Next HiFi PCR mix (NEB). Amplified libraries were purified using the Qiagen PCR purification MinElute kit.
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bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a concentration of 50mM and 16µl of each sample were PCR amplified using 2x NEB Next HiFi PCR mix (NEB) and 16 PCR cycles.
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bioRxiv - Cancer Biology 2020Quote: ... The promoter region and nanoluciferase coding region was PCR amplified from the pNL1.1 reporter constructs using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), and 400 nM of forward and reverse primers (pNL1.1_forward 5’-AATTATCTTAAGATTTCTCTGGCCTAACTGGCCGG and pNL1.1_reverse 5’-AATTATCTTAAGTGGGTTGAAGGCTCTCAAGGGCATC) ...
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bioRxiv - Zoology 2021Quote: ... The PCR mixtures for first step and nested PCR step (12.5 μl) contained 1X OneTaq Hot Start Master Mix (NEB) and 0.2 μM of each primer ...
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bioRxiv - Genomics 2020Quote: ... a PCR reaction was run on the diluted sample using 25μL Q5 Hot Start PCR Master Mix (M0494S, NEB) and 2.5μL (20μM ...
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bioRxiv - Genetics 2022Quote: ... All PCR reactions were conducted using Q5 High-Fidelity 2X PCR Master Mix following the manufacturer’s directions (New England Biolabs). These fragments were transformed into S ...
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bioRxiv - Cancer Biology 2021Quote: PCR amplifications were carried out using Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Cat#M0531L) according to the manufacturer’s instructions (25 µl reaction) ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial 16S rRNA V4 fragments were PCR amplified using NEBNext Q5 Hot Start HiFi PCR master mix (NEB, U.K.) with universal 16S rRNA V4 primers [564F (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-AYTGGGYDTAAAGNG ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed using NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) following the manufacture’s protocol unless specified differently ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... DNA amplification via PCR was performed using a large-scale PCR system using Q5 polymerase (New England BioLabs, USA) and resulted in 2780 bp linDNA amplicon expression cassettes ...
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bioRxiv - Molecular Biology 2022Quote: ... and a PCR with 10-13 cycles was performed using the NEBNext High Fidelity 2X PCR Master Mix (NEB) and Ad1_noMX and Ad2.1–2.12 barcoded primers described in (30) ...
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bioRxiv - Molecular Biology 2022Quote: ... the products were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs) with 200 nM of Illumina multiplex primer and 200 nM of Illumina index barcode primers (98°C for 10 sec pre-denaturation followed by 12 cycles of 98°C for 5 sec ...
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bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
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bioRxiv - Genomics 2022Quote: ... Purified genomic DNA was directly amplified by 22 cycles of PCR using NEBNext Ultra II Q5 PCR MasterMix (NEB). Sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
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bioRxiv - Genetics 2023Quote: ... The PCR reaction was performed using NEBNext® High-Fidelity 2× PCR Master Mix (New England BioLabs, catalog #M0541) with a reaction volume of 10 ul including 5 ul 2× PCR Master Mix ...
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bioRxiv - Genetics 2023Quote: ... The target loci were PCR amplified in 30 PCR cycles using Q5 High-Fidelity DNA Polymerase (New England Biolabs), locus-specific primer pairs (Supplementary Information ...
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Plant Biology 2024Quote: ... The sequencing libraries were prepared by PCR amplification using the NEBNext PCR master mix (New England Biolabs, no. M0541S). The amplified libraries were purified with AMPure beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
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bioRxiv - Genomics 2023Quote: ... Accessible DNA libraries were amplified by PCR using NEBNext High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), custom Nextera PCR primers as previously described (29) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reactions contained 2 μl cDNA in 50 μl PCR reaction with Phusion Hot Start Flex (New England Biolabs). Reaction conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene cat#52961) by PCR amplification using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...