Labshake search
Citations for New England Biolabs :
1751 - 1800 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... 2.5 µl 25 µM Custom Nextera PCR Primer 2 and 25 µl NEB Next High Fidelity 2x PCR Master Mix (NEB, #M0541) with 1 cycle of (72°C for 5 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µl of DNA isolate was used for PCR (primers are in supplementary table 1.4) and PCR cleanup was performed using Exo-SAP (NEB, M0293, M0371). Sanger Sequencing reactions were prepared using (Thermo Fisher # 4337455) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR product was denatured and annealed in an 18 μl reaction (15 μl PCR product, 2 μl NEB Buffer2 (10X), 1 μl nuclease-free H2O ...
-
bioRxiv - Genetics 2021Quote: ... 200-800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR (Gloor et al., 1993) using OneTaq PCR master mix (NEB #M0271L). PCR conditions were 95°C for 30 seconds ...
-
bioRxiv - Synthetic Biology 2022Quote: ... DNA fragments to be assembled were amplified by PCR using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532S), purified with gel electrophoresis and Zymoclean Gel DNA Recovery Kit (Zymo Research ...
-
bioRxiv - Systems Biology 2022Quote: ... PCRs for screens with the vTR and CoV libraries were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541L) with 33 cycles ...
-
bioRxiv - Genetics 2023Quote: ... were genotyped by amplifying the fourth coding region of EDNRB1 via PCR and digesting the PCR products with the restriction enzyme BsrI (New England BioLabs, #R0527S). Both deletions eliminate cut sites for BsrI and can thus be genotyped simultaneously (S1 Fig) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The PCRs were conducted in volumes of 30 μL comprising a Phusion® High-Fidelity PCR Master Mix (New England Biolabs) (15 μL) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Eluted fragments were amplified by PCR with an appropriate number of PCR-cycles (5-8) using Custom NExtera PCR primers50 and the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S). Amplified DNA was purified using Qiagen MinElute Kit (Qiagen ...
-
bioRxiv - Genetics 2023Quote: ... was genotyped by amplifying the fifth coding region of EDNRB1 via PCR and digesting the PCR products with the restriction enzyme SmaI (New England BioLabs, #R0141S). The R315P variant creates a cut site for SmaI (S2 Fig) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... After confirming the PCR product size with gel electrophoresis the PCR product was incubated with DpnI (NEB, Ipswich, MA, Cat #R0176S) for 1 hour at 37°C to digest the plasmid template ...
-
bioRxiv - Molecular Biology 2023Quote: ... ATAC-seq libraries were then prepared using Custom Nextera PCR primers and NEBNext High-Fidelity 2x PCR Master Mix (NEB, #M0541).
-
bioRxiv - Cancer Biology 2023Quote: ... Transposed DNA was then purified on Diapure columns (Diagenode, Transposed purified DNA was then pre-amplified for 5 PCR Cycles using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) and Illumina indexing primers ...
-
bioRxiv - Biochemistry 2024Quote: ... Truncated EWSR1 constructs were created by PCR-based cloning from the His-MBP-EWSR1 expression vector using inverse PCR with Phusion DNA polymerase (New England Biolabs, F530S) then DpnI digest (New England Biolabs ...
-
bioRxiv - Plant Biology 2020Quote: ... The nuclei were washed two times with a plastid-lysis buffer (NEB + 2% triton) followed with centrifugation (5 min at 1500 g ...
-
bioRxiv - Developmental Biology 2020Quote: ... eluted in 20 μl of water and PCR amplified using 25 μl NEB Next High-Fidelity 2x PCR Master Mix (NEB, #M0541 L), 2.5 μl of P5_BRB primer (5 μM ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 µl of genomic DNA was used for screening by PCR amplification (Phusion® High-Fidelity PCR Master Mix with HF Buffer, NEB, M0531S) of the targeted genomic region ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 ∼ 1.5 μl of DNA extract was added to 20 μl of PCR reaction mix containing standard PCR buffer and 1 unit of Taq DNA polymerase (Cat # = M0273, New England Biolabs, Beverly, MA). For long-range PCR ...
-
bioRxiv - Genetics 2020Quote: ... All the PCR was performed using NEBNext® High-Fidelity 2X PCR Master Mix (Catalog number: M0541L, New England Biolabs Inc., USA) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: ... and the library was amplified 4 cycles by PCR using Phusion high-fidelity PCR master mix with HF buffer (New England Biolabs cat. #M0531L). Finally ...
-
bioRxiv - Microbiology 2023Quote: ... The fosA PCR product was cloned into the inverse PCR product from pCD13pSK using NEbuilder Hifi assembly (New England Biolabs, Ipswich, MA). Both fosA complemented derivatives of Mu208Δ fosA and Mu582 were created by incorporating the fosA gene along with a 200bp upstream region from the Mu208 genome into the att site of each genome (12).
-
bioRxiv - Synthetic Biology 2022Quote: The detection in the specific primer amplification experiments was performed with a lower concentration of LbCas12a (1μM, NEB, Cat. # M0653S). Interestingly ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 2) library for miRNAs detection utilizing NEBNext® Small RNA Library Prep Set for Illumina (New England Biolabs (UK) Ltd ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10 μL RNA was mixed with 2 μL of 100 μM PvG748-SBS12-RT random hexamer primer (IDT) and 1 μL of 10 mM dNTP mix (NEB). The sample was incubated for 3 min at 65 °C and transferred to ice ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from total RNA using the rflP specific primer (P-LCM621) and template-switching oligonucleotide (P-LCM619) using RT Template Switching Enzyme Mix (New England Biolabs). The 5’-end end of the rflP cDNA was enriched by PCR using the primers P-LCM620 and P-LCM622 ...
-
bioRxiv - Biochemistry 2020Quote: ... The coupling reaction was allowed to proceed at RT for 45 minutes and was followed by addition of apyrase (0.0125 Units, NEB Inc.) to hydrolyse residual GDP and maintain the high-affinity nucleotide-free state of Gs ...
-
bioRxiv - Bioengineering 2021Quote: ... Human codon-optimized retron RTs were synthesized and inserted into EcoRI-digested pHG023 backbone or SmaI-digested pHG077 backbone by NEBuilder (New England Biolabs). The cloning of plasmids for expressing 5’ rgRNA and 3’ rgRNA were based on pHG215 backbone ...
-
bioRxiv - Microbiology 2020Quote: Complementary DNA (cDNA) synthesis and qPCR were carried out using LunaScript RT Supermix and Luna UniveRsaL qPCR Master Mix (New England BioLabs), respectively ...
-
bioRxiv - Microbiology 2020Quote: ... by PCR amplification of cDNA generated using reverse transcribed from total cellular RNA isolated from C636 Aedes albopictus cells using Protoscript II RT (NEB) and oligo-dT primers (IDT) ...
-
bioRxiv - Genomics 2022Quote: ... Poly-A mRNA was eluted in 36 μl of nuclease free water and 12 μl was reverse transcribed in a 30 μl reaction using the Template Switching RT Enzyme Mix (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... according to the manufacturer’s recommendations and subjected to ligation (2h at RT) with a pre-adenylated 3’ linker (2µM final) and a truncated T4 ligase (NEB M0373L). Ligated RPFs of 50 – 70 nucleotides (nt ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA was mixed with gene-specific primers and subjected to RT-qPCR using Hot Start Taq-based Luna qPCR master mix (NEB). The reaction was run on a Bio-Rad CFX Real Time PCR system ...
-
bioRxiv - Microbiology 2021Quote: ... by PCR amplification of cDNA generated using reverse transcribed from total cellular RNA isolated from C636 Aedes albopictus cells using Protoscript II RT (NEB) and oligo-dT primers (IDT) ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB], 30 U ProtoScript II Reverse Transcriptase [NEB]) were added ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were then subjected to library RT using random octamers (63) and to cDNA amplification thereafter using the Phusion High-Fidelity DNA Polymerase (New England Biolabs). A forward primer sequence against the 5’ Illumina adapter (63 ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA was generated from RNA (isolated of supernatant of Omicron isolate of RD infected cells) by LunaScript RT SuperMix (NEB). PCR reactions with Q5® High-Fidelity DNA Polymerase (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs, New England Biolabs, Ipswich, MA, USA) and CFX96 (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... and RT‒qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs, New England Biolabs, Ipswich, MA, USA) and CFX96 (Bio-Rad ...
-
bioRxiv - Plant Biology 2023Quote: ... And 1 µg of the total RNA was reverse transcribed into cDNA using Luna script RT Supermix (New England Biolabs) as per the manufacture’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
-
bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we first mixed 10.5 uL of purified RNA with 2 uL of 2 uM RT primer (AGGGACATCGTAGAGAGTCGTACTTANNNNNNNNNNAGATGAACTTCAGGGTCAGC, where Ns comprise the UMI) and 2uL of 10mM dNTP mixture (NEB), incubated the mixture at 65C for 5 minutes ...
-
bioRxiv - Immunology 2024Quote: Reverse-transcription on total RNA (100 to 500 ng) was performed with random hexamer/oligo-dT-Primer containing LunaScript RT Supermix (New England Biolabs) following the manufactureŕs instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR was done with Q5 High-Fidelity Polymerase (NEB) following the manufacturer’s instructions and in the presence of 10% DMSO ...
-
bioRxiv - Microbiology 2020Quote: ... Genotype analysis PCR was done using Phusion polymerase (NEB). For P ...
-
bioRxiv - Synthetic Biology 2021Quote: The PCR product was then circularized with electroligase (NEB) and transformed with high efficiency (≥ 1.5 million CFU/mL ...
-
bioRxiv - Synthetic Biology 2021Quote: ... After PCR amplification samples were digested with DpnI (NEB) restriction enzyme to remove excess pUC19 template.
-
bioRxiv - Immunology 2021Quote: ... Sequences were amplified by PCR using Phusion polymerase (NEB). Amplicons were separated on 1.0 -1.8 % agarose gel ...
-
bioRxiv - Genetics 2021Quote: ... PCR products were generated by Phusion (New England Biolabs) PCR using pDEW201-PiraD as template and the primer pairs 5’-GGGGCGGCCG TTGCTGACAG GATTCAGGCC TGTCTC-3’ and 5’-GGGGCGGCCG AAACCTTACT TGCCTATGAA TATCTA-3’ for pDEW201-P1iraD and 5’-GGGGGGCCCA ATAATGCCTG TGAATGGTAT TTTTG-3’ and 5’-GGGGGGCCCG ACCTCAATAT AGCAACATCA AATTC-3’ for pDEW201-P2iraD ...