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Citations for New England Biolabs :
1451 - 1500 of 2065 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Developmental Biology 2023Quote: ... 25 uL of 2xphusion PCR mastermix (Phusion High-Fidelity PCR Master Mix with HF Buffer – 100 rxns, NEB, cat #M0531S), 0.625uL of 100uM F primer ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg of cDNA was amplified with Phusion polymerase for 32 cycles and separated on a standard 1% agarose gel together with a 100 bp or 1 kb ladder (New England Biolabs). Ribosomal protein L32 (RpL32 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Genetics 2023Quote: ... libraries were generated using total RNA from single and pooled mites (100-600 ng) with the NEBNext Multiplex Small RNA Library Prep kit (NEB) following the manufacturers protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The reserved half of the stored fixed cells were pelleted and resuspended in 100 μL Klenow extension buffer (1X NEB buffer 2 ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Total amount of 100 ng of small RNA or mRNA was digested with a Nucleoside Digestion Mix (New England BioLabs) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was eluted at 100% Buffer F and protein fractions were pooled and combined with TEV and CIP (#M0525) from NEB and dialyzed overnight against Buffer E at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... containing the sgRNA sequences were resuspended at 100 nM in H2O and amplified by PCR using HF Phusion polymerase (New England Biolabs). After verifying amplification on a 10% acrylamide gel ...
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bioRxiv - Genomics 2022Quote: ... The eluted ATAC-Seq library was cloned into AgeI- and SalI-digested pLenti-STARR using a 3:1 molar ratio (insert:backbone) in a total reaction volume of 100 μL using the NEBuilder HIFI DNA Assembly Kit (NEB #E5520S). The reaction was concentrated to a total of 20 μL in a Reaction Cleanup Kit (Qiagen #28206) ...
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bioRxiv - Developmental Biology 2023Quote: ... MNase digestion was performed in 100 µl of digestion buffer containing 1 mM CaCl and 40 U of Mnase (New England Biolabs) at 24°C for 15min while shaking ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then reverse crosslinked and lysed by adding 10μL of 10X Lysis-T (250mM EDTA, 2M NaCl, 10% Triton X-100) and 4μL of proteinase K (NEB, P8107S) and incubating at 55°C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide bound beads were washed with 100 µL 1x lambda phosphatase buffer prior to incubating with lambda protein phosphatase (P0753; NEB).
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bioRxiv - Evolutionary Biology 2023Quote: ... We then quantified DNA concentrations using a Qubit fluorometer and submitted 100 ng DNA to Genome Quebec where technicians prepared shotgun DNA libraries using the NEB Ultra II (New England Biolabs) library preparation kit and then sequenced the libraries using four NovaSeq6000 S4 PE150 lanes.
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bioRxiv - Plant Biology 2023Quote: ChIP-seq libraries were constructed from 100 ng of DNA samples using the NEB Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... libraries were prepared for all samples following the epiGBS2 protocol42,43 by digesting 100 ng of gDNA with restriction enzymes NsiI and Csp6I (New England Biolabs, UK). Individually barcoded hemimethylated adapters ...
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bioRxiv - Microbiology 2023Quote: ... biwaensis Akk2750 (100% sequence match to CSUN-19) (38) with Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and incorporating a C-terminal HA epitope tag ...
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bioRxiv - Bioengineering 2023Quote: ... genomic loci were amplified in a first PCR reaction (PCR-1) reaction from approximately 100 ng of gDNA using Q5 High-fidelity DNA Polymerase (NEB) and the primers listed in Sup ...
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bioRxiv - Cell Biology 2023Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without 1 U Endo H (NEB, Frankfurt, Germany). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Microbiology 2024Quote: The msdDNA cDNA was isolated from acrylamide gels and 100 ng was used to extend the 3’ end with dCTP or dGTP and terminal deoxynucleotidyl transferase (TdT) from NEB, according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 15 µl of genomic DNA (DNA concentration is around 100 ng/µl) were digested or mock with 20 units of BsrGI or BamHI restriction enzymes (New England BioLabs) for 5 hours at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) with minor modifications ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from each homogenized pool of 100 flies using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA quality was assessed using Nanodrop and quantified with Qubit ...
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bioRxiv - Cell Biology 2024Quote: ... Size-selected DNA fragments were amplified using NEB unique multiplexed i5 and i7 primers (E6440) in a total reaction volume of 100 µl together with 2 U Phusion High-Fidelity DNA Polymerase (NEB) and in the presence of 0.2 mM dNTPs and 1X Phusion High-Fidelity buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Ligation was performed on a 7-fold dilution of HindIII-digested chromatin using 100 units of Quick T4 DNA ligase (New England Biolabs) at 16°C for 16 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of genomic DNA (∼100 ng) was mixed with 8 µl of 1x Cutsmart buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of genomic DNA (∼100 ng) was mixed with 8 µl of 1x Cutsmart buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biophysics 2021Quote: ... following incubation for ∼2 hours of 10 mg/ml BSA (New England Biolabs) diluted in buffer A containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were transferred to 7 ml of 1.15x T4 ligation buffer (NEB), incubated with 1% Triton X-100 for 1 hour at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sections were treated with 25µl/ml DNAase I (REF-M0303S, BioLabs, New England) in DNase I buffer (pH 7.9 40mM Tris-HCl ...
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1.2 mL of 1X DpnII buffer (NEB, Ipswich, MA), transferred to a VK05 tubes (Precellys ...
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bioRxiv - Molecular Biology 2021Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Molecular Biology 2019Quote: ... XPG solution was mixed with 10 ml of amylose resin (New England BioLabs) pre-equilibrated in dialysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Peak fractions were pooled and passed over an 8 mL amylose column (NEB), washed with 5 CV of Amylose Wash Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... and proteins were de-glycosylated with PNGase F (500,000 U/ml, New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 1x EvaGreen (Biotum) and 20 U/ml Q5 DNA polymerase (New England Biolabs). The reaction components were prepared as a 2x-master mix and 5 µl of the mix was pipetted into opaque wells of 384-well PCR-plate (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mL of ligation mix (500 μL of T4 Ligase Buffer 10x (NEB), 100 μL of T4 DNA Ligase (400 U/μL ...
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bioRxiv - Genomics 2021Quote: ... polony gels were digested with 800 unit/mL (final concentration) TaqI (NEB R0149) diluted in 1× CutSmart buffer at 60°C for 1 hour.
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bioRxiv - Microbiology 2021Quote: ... and sometimes included up to 0.01 mg/mL bovine serum albumin (B9000S, NEB) to limit enzyme adsorption to pipettes and tubes ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl BSA (20 mg/ml) (New England Biolabs Inc., Ipswich, MA, USA), 10 μl Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... followed by digestion with 55 µL of proteinase K (NEB, 20 mg/mL) for 30 min at 50 °C ...
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bioRxiv - Biophysics 2021Quote: ... following passivation for ~2 hours of 10 mg/ml BSA (New England Biolabs). After removing non-adhered BSA by washing the flow cell with PBS ...
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bioRxiv - Neuroscience 2019Quote: ... sections were treated with DNase I (10 U/mL, New England Biolabs, M0303S) for 1 h at 37°C and rinsed in PBS (3 times ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in the final concentration of 30 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 μl of proteinase K (NEB, 800 units/ml) for 20 min at 37 °C and 5 ul of each sample was applied onto 20% denaturing gels (20% acrylamide/bis-acrylamide ...
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bioRxiv - Molecular Biology 2020Quote: ... Clear lysate fraction was added to 1.5 ml magnetic chitin beads (NEB E8036S) equilibrated in lysis buffer ...
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bioRxiv - Genomics 2021Quote: ... the PCR reactions were treated with 200 U/ml of Exonuclease I (NEB) for 1 h at 37°C ...
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bioRxiv - Genomics 2021Quote: ... tissues were treated with 1.5uL RNase H (5,000 units/ml; New England Biolabs) in 50uL final volume in 1X RNAse H buffer at 37°C for 2 hours and washed three times with Stellaris wash buffer at room temperature for 10 minutes before mounting and imaging.
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bioRxiv - Genomics 2021Quote: ... the PCR reactions were treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...