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Citations for New England Biolabs :
1451 - 1500 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The amplified plasmid containing the mutated p66 was double digested with NdeI and XhoI restriction enzymes (New England Biolabs), gel purified and cloned into MCS-2 of the pETDuet-1 vector pre-digested with the same set of restriction enzymes and carrying mutated p51 domain in MCS-1 ...
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bioRxiv - Microbiology 2020Quote: ... IVT gRNA was produced from SFV infectious clone plasmid and treated with 10 U of DNase1 (RNase-Free; NEB) for 30 min at 37°C before purification with the RNeasy MiniElute Cleanup kit (Qiagen ...
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bioRxiv - Developmental Biology 2019Quote: ... and the Tol2-5xUAS-TATA:M2H37A Bleeding Heart plasmid was assembled from the digested pBH and 5xUAS-TATA:M2H37A PCR product using Gibson Assembly Master Mix (NEB). The entire plasmid was sequenced to confirm ligation fidelity.
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bioRxiv - Cell Biology 2019Quote: ... were modified based on the pcDNA3.1(+)-C-I30-FLG-TEV plasmid via the Q5 Site-Directed Mutagenesis Kit (cat#: E0554S, NEB). pCMV-FLAG-ABCE1 was a gift from Dr ...
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bioRxiv - Bioengineering 2019Quote: ... plasmid pRM03-Spec was linearized by AgeI and simultaneously dephosphorylated by Shrimp Alkaline Phosphatase (New England Biolabs, Cat. # M0371S). A 4.2 kb PCM expression cassette was PCR amplified form plasmid pPCM using primer pair MMorCF-AgeIfor/newPCFAgeIrev and double digested with DpnI/AgeI ...
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bioRxiv - Biochemistry 2019Quote: ... Methylation assays were performed using 100 ng of an unmethylated pUC19 plasmid purified from dam- /dcm- E.coli strain (C2925I, NEB) or a 1-kb fragment containing 14 CpG sites amplified from the Meis1 enhancer were used as DNA substrates for filter binding assays ...
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bioRxiv - Biophysics 2019Quote: Double-stranded DNA was synthesized through PCR amplification of a 1.565-kbp segment of the pBR322 plasmid (New England Biolabs), using forward and reverse primers conjugated with a 5’ biotin and a 5’ digoxigenin ...
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bioRxiv - Cancer Biology 2019Quote: A deletion between amino acid 336 and the beginning of GFP was generated from the MCSV:GPR68-GFP plasmid using the Q5 mutagenesis kit (NEB).
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bioRxiv - Synthetic Biology 2019Quote: ... A Gibson assembly was carried out to link all DNA fragments and assemble the final integrative pHO_pCMV_CggR_ble plasmid using the NEB Gibson assembly kit (New England Biolabs, MA, US). 5 µl of reaction mix was transformed into chemical competent E.coli DH5alpha cells ...
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bioRxiv - Biochemistry 2021Quote: ... Yeast surface display plasmid pJYDC1 (Adgene ID: 162458) and pJYDC3 (162460) were cleaved by NdeI and BamHI (NEB, USA) restriction enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... clr4 gene specific sgRNA was cloned into Cas9 containing pLSB-KAN plasmid by Golden Gate Assembly kit (E1601S, NEB). The resulting plasmid clr4-pLSB-KAN and clr4 HR template obtained by annealing primer pair WW748-clr4-HR-F/WW749-clr4-HR-R (Table S3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The 36N plasmid DNA library was generated using a Q5 site directed mutagenesis kit (New England Biolabs, Ipswitch, MA). For amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... and KpnI) into pSB/IR-CA-HybIntr-SV40intr-BleoR-T2A-mCherry plasmid restricted with restriction endonucleases BsaI (NEB, USA) and AsiG.
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bioRxiv - Bioengineering 2021Quote: ... 20 ng of extracted plasmid DNA was added to a 50 µL PCR reaction with Q5 DNA polymerase (NEB) for 16 cycles annealing at 53 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pES101 (encoding GPx7) was constructed by assembling three DNA fragments using the Gibson Assembly Kit (New England Biolabs). The DNA fragments used were a fragment amplified from pcDNA3.1+ (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... PCR fragments were inserted into linearized NaV channel expression plasmids by Gibson Assembly using NEBuilder HiFi Assembly Mix according to the manufacturer’s protocol (New England BioLabs). Introns were inserted after c.2946 (exon 15/16 junction ...
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bioRxiv - Genomics 2021Quote: ... non integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 minutes heat inactivation at 65 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The pDD162 plasmid was used to deliver Cas9 and sgRNAs modified using the Q5 Site-Directed Mutagenesis Kit (NEB) for sequence specific CRISPR editing of each gene ...
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bioRxiv - Plant Biology 2021Quote: ... and eGFP were cloned into the digested plasmid according to NEBuilder HiFi DNA Assembly protocol (New England Biolabs, USA). The assembled entry construct was then cloned into the broad host-range vector pHM1 by the Gateway cloning (Supplemental Figure 2 ...
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bioRxiv - Systems Biology 2021Quote: ... Each construct was then randomly barcoded by digesting the plasmid with XbaI followed by HiFi assembly (New England Biolabs) with a single-stranded oligo containing 16 random N’s (TRIP barcodes ...
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bioRxiv - Microbiology 2021Quote: ... were used to amplify the pQE60- ompA recombinant plasmid (Size- 4.5 kb) using Phusion high fidelity DNA polymerase (NEB). The reaction mixture was heated at 950C for 10 minutes for the plasmid’s denaturation ...
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bioRxiv - Microbiology 2020Quote: ... 107 epimastigotes were electroporated with 2.5 µg of pTRIX2-GFP plasmid (Fig 1 A) linearized with AscI and SacI (NEB). The plasmid had been derived from pTRIX-REh9 [28] ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNAs were then ligated with the digested plasmid using the T4 DNA ligase and buffer (New England Biolabs). Reactions were carried out following the Zhang Lab General Cloning Protocol (Addgene).
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biophysics 2020Quote: ... T270 plasmid was digested by HpaI at 37°C for 4 hr in the CutSmart buffer (New England BioLabs) to place the (TTAGGG)270 at the middle region of the linearized substrate ...
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bioRxiv - Genomics 2022Quote: ... and cloned into the STARR-seq plasmid with NEBuilder® HiFi DNA Assembly Cloning Kit (E5520S, New England Biolabs) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The pLVX-StrepII-ORF-IRES-Puro plasmids were then cloned using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) from the pTriEX-6 plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... P301L/V337M)-EYFP plasmids after the Tau sequence using the Q5 site directed mutagenesis (SDM) kit (New England Biolabs). Tau fragments were subcloned into pcDNA3.1 by restriction digestion and further into pCW57.1- MAT2A all-in-one tet-off lentiviral backbone (a gift from David Sabatini (Addgene plasmid # 100521))71 by Gibson assembly ...
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bioRxiv - Cancer Biology 2022Quote: ... Three sgRNAs designed against Rbms3 were cloned into the pLL3.3 sgRNA-CRE vector by modifying the original sgLKB1 plasmid with the Q5 Site-Directed Mutagenesis kit (NEB). The following sgRNAs against Rbms3 were used and pooled to make 2 lentiviruses.
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bioRxiv - Biochemistry 2022Quote: ... 1x arrays DNA were cut from carrier plasmid DNA by incubation overnight with 5,000 units of EcoRV-HF restriction endonuclease (New England Biolabs) in 1x CutSmart Buffer (20 mM Tris,OAc ...
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bioRxiv - Synthetic Biology 2022Quote: ... in which 20-50 ng of uncut acceptor plasmid and a 2:1 molar ratio of donor plasmid or PCR products were mixed together with 1.5 μl DNA T4 ligase buffer (#B0202S; NEB), 0.0015 mg BSA (B9000S ...
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bioRxiv - Synthetic Biology 2022Quote: ... spacer sequences were cloned into pCAST and pHELIX plasmids via Golden Gate assembly with SapI (New England Biolabs, NEB). Target site features for all gRNAs used in this study are found in Supplementary Table 2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... spacer sequences were cloned into pCAST and pHELIX plasmids via Golden Gate assembly with SapI (New England Biolabs, NEB). Target site features for all gRNAs used in this study are found in Supplementary Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated from previously described pcDNA3-FLAG-MS2-TTP plasmids (28) using site-directed mutagenesis according to manufacturer’s protocol (New England Biolabs). Expression plasmids for FLAG-tagged constitutive active and catalytically dead MK2 kinases were previously described (12 ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids encoding Cdiff RNAP mutants (rpoC K84E, K84R, and K84Q) were constructed by Q5 site-directed mutagenesis (NEB) using pXC.026 as the template.
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bioRxiv - Biochemistry 2022Quote: ... Cleavage reactions were performed by combining TtAgo complexes with either 79.85 fmol of linearized KAC833 plasmid substrate (digested with PvuI, NEB) or 79.85 fmol supercoiled plasmid DNA (KAC1151 or MNW95 ...
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bioRxiv - Cell Biology 2022Quote: ... All plasmids used in this study were constructed using either Q5 Site Directed Mutagenesis Kit (New England Biolabs, E0554S) or In-Fusion HD Cloning Plus (Clonetech ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid backbone, pFA6 (Janke et al., 2004) was digested using the restriction enzymes SacII (#R0157S, New England Biolabs) and HindIII (#R3104T ...
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bioRxiv - Microbiology 2021Quote: ... Transformants positive for each Cas9HygAMA-sgRNA plasmid were screened by colony PCR using Taq DNA polymerase (New England Biolabs) with the forward Cas9HygAMAccdB primer (MR139 ...
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bioRxiv - Immunology 2020Quote: The following plasmids used in this study have been previously described: pEF-Tak-Flag (52) and pCMV-Gluc2 (NEB). The following plasmids were generated in this study:
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bioRxiv - Microbiology 2021Quote: ... was amplified from RH genomic DNA and cloned into the pTKO2-HPT-3xHA plasmid (44) using Gibson Assembly (NEB).
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bioRxiv - Biophysics 2021Quote: ... The EGFR-mApple and EGFR-mEGFP plasmids were digested with AgeI (AgeI-HF, R3552S, New England BioLabs, Massachusetts, USA) and SpeI (SpeI-HF ...
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bioRxiv - Synthetic Biology 2022Quote: PCR-amplified parts of DNA were assembled using Golden Gate Assembly and inserted into plasmid backbones (New England Biolabs).49 The T7 driven gene circuits for fusion protein expression were inserted into vector PBS1C,50 which carries homology sites of amyE locus and a chloramphenicol resistance selection marker ...
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bioRxiv - Genetics 2022Quote: ... the sgRNA sequences were inserted into the pBSMVγPDS plasmid after removing the PDS fragment by PacI and NotI digestion (New England BioLabs, Ipswich ...
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bioRxiv - Genetics 2022Quote: ... The digested PCR products and the pBSMVγ plasmid were ligated using T4 ligase (New England BioLabs, catalog number M0202). This plasmid was named pBSMVγQT1 (Figure 1b) ...
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bioRxiv - Microbiology 2022Quote: ... and the fucI PCR product was cloned into the plasmid using the NEBuilder HiFi DNA Assembly Kit (NEB; E5520S), followed by transformation into the E ...
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bioRxiv - Microbiology 2022Quote: ... Briefly 5 µg pAc CrPV 5’UTR-1A-GFP-3’ or pCrPV-3 plasmids were linearized with Eco53KI (NEB) or 5 µg pTOPO dsRNA plasmids with EcoR1 (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then transcribed from the NsiI-linearised plasmids with the HiScribe™ T7 ARCA mRNA Kit (NEB E2065S) or HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids used in this study were linearised by digestion using the appropriate restriction enzymes (New England Biolabs, Hitchin, UK). All DNA constructs were verified by sequencing (Source Bioscience).
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bioRxiv - Biochemistry 2019Quote: To generate the plasmid pIL-SVnisT the nisT gene from pNZ-SV10HnisT was amplified by Phusion DNA polymerase (NEB) with the primer pair infupIL-SVfor and infupIL-SVrev (Supplementary File 4 ...