Labshake search
Citations for New England Biolabs :
1651 - 1700 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Homology arms were then cloned into an SEC backbone plasmid (pJF250, pDD282, or pLC019) with Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB). A mixture of the modified SEC repair plasmid (25 ng/μl ...
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bioRxiv - Biochemistry 2021Quote: AcpP was expressed from a pET28a plasmid encoding acpP with a thrombin-cleavable N-terminal 6xHis tag in C3031I cells (NEB). 2L cultures of LB supplemented with kanamycin (25 μg/mL ...
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bioRxiv - Biochemistry 2020Quote: Deglycosylation of S protein from the lysate of HEK293 cells transfected with pTwist-EF1a-nCoV-2019-S-2xStrep plasmid was performed as described previously [35] using PNGase F (New England Biolabs).
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bioRxiv - Genetics 2021Quote: ... Then these sgRNAs were annealed and ligated to the pSpCas9(BB)-2A-RFP plasmid which was digested by Bbs I (NEB) enzyme ...
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bioRxiv - Microbiology 2020Quote: ... targeting the C-terminus of the TgPPM3C gene was cloned into the p-HXGPRT-Cas9-GFP plasmid backbone using KLD reactions (New England Biolabs), as previously described [49] ...
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bioRxiv - Plant Biology 2020Quote: The cDNA encoding the full length AtHMA4 protein or the cDNA encoding the 473 amino acid AtHMA4 C-terminal domain was amplified from previously generated AtHMA4 cDNA plasmids (Mills et al., 2010) by PCR using Phusion High-Fidelity DNA polymerase (New England Biolabs). The primers AtHMA4FL-F (5′-ACTGGATCCCTCTCAACCTTTATCTGAT-3′ ...
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bioRxiv - Systems Biology 2020Quote: The extracted plasmids were amplified using “lib-seq” primers in the Supplementary Table 6 for 10 cycles using Phusion PCR kit (NEB). Each 50 μL PCR reaction consisted of 6 μL of plasmid library ...
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bioRxiv - Microbiology 2020Quote: Plasmids for the expression of the Holo-nanocompartment (DypB-loaded) and naked DypB constructs were designing using Gibson Assembly (NEB). Each construct was cloned into a pET-14-based destination vector containing a T7 promoter ...
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bioRxiv - Plant Biology 2021Quote: ... and ligated together into the gateway compatible Gene Entry Clone (GEC) plasmid (Wang et al., 2013) by using Gibson Assembly (New England Biolabs). RBP47B-DENDRA2 was cloned into pEarleyGate 100 (Earley et al. ...
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bioRxiv - Microbiology 2020Quote: Viral RNA genome for both WT ZIKV and the X1 mutant was in vitro transcribed from ligated plasmid DNA using a HiScribe T7 ARCA mRNA kit (NEB). Approximately 40 μg of the resulting mRNAs were transfected into Vero cells using MessengerMAX lipofectamine transfection reagent (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was ligated to a low-copy plasmid vector pWSK29 (BamHI/Sall) by Gibson assembly (New England Biolabs). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... were generated by PCR amplification of either full-length pfget4 or nucleotides encoding for the first 246 amino acids of PfGet2CD using PfGet4-EcoRIF (5’-GTACCGGAATTCATGAAAAAGTTCAAATTTAGTAAAGAAAAGCTAGCC-3’)/PfGet4-XhoISalIR or PfGet2CD-BamHIF (5’-GTACGCGGATCCATGGATAAAAATACATTAAAAAGAA-3’)/PfGet2CD-XhoISalIR (5’-AGACCGGTCGACCTCGAGTTATTCATGTTTCGTAATAATAAATTG-3’) primer pairs and subsequently cloning at corresponding sites in pMALc2X plasmid (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... the codon optimized RBD was PCR amplified and cloned into the bicistronic pDZ rescue plasmid system for A/Puerto Rico/8/1934 using the NEBuider HiFi DNA assembly kit (NEB). Specifically ...
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bioRxiv - Immunology 2021Quote: ... Restriction digest of pMK-RQ plasmid DNA containing EtIMP1 was performed using Bam HI and Not I restriction enzymes (New England Biolabs). Restriction digest of pYD1-EtAMA1Cit plasmid was performed with the same restriction enzymes to remove the EtAMA1 insert but retain the citrine tag ...
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bioRxiv - Microbiology 2021Quote: ... a 1kb fragment up- and downstream of the target gene was amplified via PCR and cloned into the pMQ30 plasmid using Gibson cloning (NEB) and transformed into E ...
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bioRxiv - Immunology 2021Quote: ... pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). 293T cells were transfected with 3.75 μg pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G ...
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bioRxiv - Immunology 2021Quote: ... The resultant DNA was cloned into a pUC19 plasmid and in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs). After DNAseI digestion and clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 S R403T and RaTG13 S T403R/T403A mutant plasmids were generated using Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Synthetic Biology 2021Quote: ... Divergent 5’ phosphorylated primers carrying the mutations were used to amplify the whole plasmid and introducer the mutations using the standard Phusion DNA polymerase protocol from NEB. Mutagenesis primers are listed in Supplementary Table 5) ...
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bioRxiv - Synthetic Biology 2021Quote: ... the templates were prepared by polymerase chain reaction (PCR) amplification from corresponding plasmid constructs using Q5 DNA Polymerase Master Mix (NEB). The PCR reaction was worked up using a Qiagen PCR purification kit ...
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bioRxiv - Microbiology 2019Quote: ... the RH-Luc+/Δgra45 parasites were co-transfected with plasmids containing sgRNAs specifically targeting the UPRT locus and SalI (New England Biolabs)-linearized pUPRT::GRA45HA plasmid at a ratio 1:5 of sgRNAs to linearized plasmid ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 1500-3000 bp of the 3’ coding sequence of each gene was amplified from RH genomic DNA and cloned into the pTKO2-HPT-3xHA plasmid (11) using either Gibson Assembly (NEB) or by cloning into the EcoRV and NotI restriction sites.
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bioRxiv - Biochemistry 2021Quote: All plasmids were constructed by Gibson Assembly from PCR products generated using Q5 Hot Start DNA Polymerase (New England Biolabs) or Phusion Polymerase (generated in-house) ...
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bioRxiv - Cell Biology 2021Quote: MBP-M18BP1.S-161-580 and MBP-M18BP1.S-161-580 SANTA were independently expressed from modified pMal-c2x plasmids (NEB) in BL21 (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The diluted phosphorylated oligonucleotide duplex was ligated into the dephosphorylated px330 plasmid using a T4 ligase (New England Biolabs, M0202M). Next ...
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bioRxiv - Cell Biology 2021Quote: ... The digested plasmid was mixed with the different PCR-amplified sensory domains-ORFs containing overlapping ends using the Gibson Assembly method (NEB). AtLEA4-2 ...
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bioRxiv - Biophysics 2021Quote: SARS-CoV-2 S N501Y plasmid was obtained from SARS-CoV-2 S plasmid (HDM-IDTSpike-fixK) by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). SARS-CoV-2 S and SARS_CoV-2 S N501Y pseudotyped retroviral particles were produced in HEK293T cells as described previously (29) ...
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bioRxiv - Cancer Biology 2021Quote: ... vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23) by digesting the plasmid and the annealed primer pair using EcoRV (NEB, #R0195L) and HindIII (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... The fragments were cloned into the srpHemo plasmid using an Infusion HD cloning kit after its linearization with NotI (NEB).
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bioRxiv - Bioengineering 2022Quote: ... Linearized plasmids were used as templates and RNA products were then produced using the HiScribe T7 RNA Synthesis Kit (NEB) per manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... a kind gift from Jelena Jakovlievic) and cloned into a T7 promoter-containing plasmid digested with EcoRI and HindIII using Gibson Assembly (NEB). The resulting plasmids were then digested with HindIII and run-off transcription was performed using the MEGAscript T7 kit (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: 1.0 pmol of oligo pool and 0.005 pmol linearized plasmid were mixed with NEBuilder® HiFi DNA Assembly Master Mix (E2621, New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: Saturation mutagenesis of mEosEM at His63 was performed in the pEGFP-N1-mito-mEosEM plasmid with Q5 high fidelity polymerase (New England Biolabs). The amplified fragments containing homologous arms and mutation sites were transformed into the Top10 competent cells (Tsingke ...
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bioRxiv - Genetics 2022Quote: ... The primers used to amplify the pEIA plasmid were pEIA-Fgibson and pEIA-Rgibson (S2 Table) and the PCR reaction was performed using Phusion polymerase (NEB). The ORF of the puromycin resistance gene was amplified using Phusion polymerase and pEA-PAC as a template and the primer pair used for the PCR reaction were PAC-Fgibson-AscI/PAC-Rgibson-NcoI (S2 Table) ...
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bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
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bioRxiv - Genetics 2022Quote: ... We used this synthetic HIS3 fragment and BsmBI-v2/HpaI-digested PFA0055 to create plasmid PFA0227 by isothermal assembly cloning using the HiFi Assembly Cloning Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the EcoRI to KpnI fragment was subcloned to a temporary plasmid and PCR amplified with Q5 high fidelity DNA polymerase (NEB) using the dCore-BamHI-FW (5’-TTT CTG GAT CCT TGC TGG CCC TGC TGT CCT GCA TC-3’ ...
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bioRxiv - Genetics 2022Quote: ... We produced linear DNA transformation fragments by digesting TFT-containing plasmids with SacI and BglII and gel purifying the fragments (Monarch Gel Purification, NEB). Genomic integration of each linear transformation fragment results in deletion of the LYP1 gene ...
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bioRxiv - Immunology 2022Quote: ... The plasmid at 10-25 ng/μl concentration together with 1 μl I-SceI meganuclease and NEB buffer (NewEngland BioLabs) were co-injected into the blastomere at one-cell stage embryos ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB) or the mEos3.2 coding sequence using InFusion (Clontech) ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... we digested the p416-UbMpro(lib)-B112 plasmid upstream of the N18 sequence and downstream of the Mpro sequence with NotI and SalI enzymes (NEB). The resulting 1800 bp fragment containing the barcoded library was isolated by Blue Pippen selecting for a 1 to 4 kB range ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Molecular Biology 2022Quote: ... fluorophores were prepared by PCR amplification from the pTriEx-MLucV plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The proteins were expressed and purified in a similar way to MLucV ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested PCR products were then ligated to the digested pcs2+MCS-P2A-sfGFP plasmid using T4 DNA ligase (M0202S, NEB) in a 3:1 insert:backbone ratio ...
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bioRxiv - Genetics 2022Quote: ... the TSS/Promoter region was amplified by PCR using Illumina compatible primers (TE127 and TE111) from 5 ng plasmid using Phusion HF polymerase (NEB). Sequencing results were analyzed using custom Python scripts to quantify the frequency of each 7 nt TSS sequence.
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bioRxiv - Microbiology 2022Quote: ... The ORF was removed by inverse PCR to generate the genomic deletion vector (P5/P6) and plasmid recircularized from PCR product (KLD enzyme blend, NEB).
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bioRxiv - Genetics 2022Quote: A DNA template for in vitro transcription was generated by PCR using oligos TE122 and TE126 on plasmid pCT-TE2 and Phusion DNA polymerase (NEB) in HF buffer (Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: The purified insert was digested with XhoI and NcoI and inserted into the plasmid by ligation with T7 DNA ligase (NEB) following the manufacturer’s instructions to generate the pGL_CXCL8_promoter_Firefly.
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bioRxiv - Microbiology 2022Quote: ... containing a T7 polymerase promoter flanking either side of the amplicon and directly cloned into a pTOPO plasmid using EcoR1 (NEB). FLuc plasmid was described previously (59 ...