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Citations for New England Biolabs :
1351 - 1400 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The HCoV-229E and FIPV nsp5 coding sequences were cloned into pMAL-c2 plasmid DNA (New England Biolabs) for expression as MBP fusion proteins containing a C-terminal His6-tag ...
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bioRxiv - Cell Biology 2021Quote: A donor plasmid was then created via Gibson assembly according to the manufacturer protocol (New England Biolabs #E2611S). Separately ...
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bioRxiv - Genomics 2020Quote: All pooled plasmid libraries were constructed by Gibson-style assembly using HiFi DNA assembly master mix (NEB E2621L) and transformed using either ElectroMAX DH10B (ThermoFisher #18290015 ...
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bioRxiv - Cell Biology 2022Quote: ... All other plasmids generated in this study were assembled by Gibson Assembly Master Mix (New England Biolabs Inc.) according to manufacturer instruction ...
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bioRxiv - Genetics 2022Quote: ... we PCR amplified NatMX from Addgene plasmid #35121 with the primers listed in Supplementary Table 6 using Phusion Hot Start Flex DNA polymerase (NEB). The NatMX cassette was transformed into the BY strain using the methods described above and transformants were plated onto YPD medium containing clonNAT ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding individual variants were pooled and electroporated into electro-competent NEB10β cells (New England Biolabs, Ipswitch, MA), which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research ...
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bioRxiv - Developmental Biology 2022Quote: ... into the linearised XhoI-AsiSI pUASt-attB-Col4α1wt-mScarlet plasmid using ligation T4 strategy (New England Biolabs, Inc.).
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bioRxiv - Biochemistry 2022Quote: ... The plasmids coding for segments 2 and 8 were digested using BbsI restriction enzyme (New England Biolab, NEB), the plasmid coding for segment 10 was linearized with BsaI (NEB) ...
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bioRxiv - Immunology 2022Quote: Plasmids containing templates for CRISPR-mediated KI were assembled using the NEBuilder HIFI assembly kit (New England Biolabs) starting from PCR amplified fragments ...
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bioRxiv - Cancer Biology 2022Quote: ... The amplified plasmids and CSII-EF MCS-mCherry vector were digested using HpaI and NotI HF (NEB, USA) restriction enzymes (Supplementary Table 2C) ...
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bioRxiv - Microbiology 2021Quote: ... Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described (42) ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was synthesized in smaller DNA fragments (500bp-3kb) (IDT gBlocks) and assembled by Gibson Assembly (NEB) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... The popZ insert and the plasmid PCR product were assembled together using the Hifi DNA assembly mix (NEB). The construction was verified by sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs, USA). The coding sequence of full length human WNK1 was derived from a commercial expression vector (#RC218208 ...
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bioRxiv - Molecular Biology 2022Quote: Bclx and CD44-v5 minigenes were cloned in EGFP-C1 plasmid following the CMV promoter using ExoIII (NEB) enzyme following manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... into the linearized AsiSI-EcoNI pUASt-attB-Col4α1wt-mScarlet plasmid using ligation T4 strategy (New England Biolabs, Inc.).
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bioRxiv - Microbiology 2022Quote: ... region between MCS-1/MCS-2 and linearized pETDuet plasmid were ligated using the Gibson Assembly® (NEB) to generate the pETDuet pe15/ppe20 plasmid ...
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bioRxiv - Genetics 2019Quote: ... The mutant plasmid pool was subcloned into the promoterless SCN5A:IRES:mCherry plasmid by restriction digest with AatII and AflII (New England Biolabs). A random 18-mer barcode was cloned into the resulting plasmid by restriction digest with Pfl23II (BsiWI ...
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bioRxiv - Evolutionary Biology 2022Quote: The DMRT regulatory region was amplified from the DMRT>GFP plasmid [28] using Phusion polymerase (New England Biolabs) with primers ...
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bioRxiv - Biophysics 2022Quote: The VcRfaH expression vector pVS13 (V. cholerae rfaH from pHC301 (Carter et al., 2004) in plasmid pTYB1 (NEB)) was a gift from I ...
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bioRxiv - Genomics 2019Quote: ... we transfected K562 cells as previously described with either 1 µg of pUC19 plasmid (New England BioLabs #N3041S); 0.5 µg of PB-SRT-tdTomato plasmid and 0.5 µg pUC19 ...
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bioRxiv - Cell Biology 2020Quote: ... Correct plasmids were linearized with PvuI and used in HiScribe™ T7 ARCA mRNA Kit (with tailing) (NEB) and purified with RNA-25 Clean & Concentrator RNA purification kit (Zymo Research).
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bioRxiv - Immunology 2020Quote: ... and the assembled plasmid pCO1 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Biophysics 2020Quote: ... The PCR-amplified sequences were ligated into a NotI- and BssHII-digested BlucB plasmid using DNA ligase (NEB) and transformed into E ...
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bioRxiv - Developmental Biology 2020Quote: ... The plasmid pDD162 used to deliver Cas9 and each sgRNA was modified (Q5 Site-Directed Mutagenesis Kit, NEB) to insert the appropriate sgRNA guide sequence for each CRISPR edit ...
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bioRxiv - Microbiology 2020Quote: ... The U6 cassette from plasmid pDC_A1G2 was amplified using Q5 high fidelity DNA Polymerase (New England BioLabs; NEB) with primers HD104_Cas9UTR_F and HD104_Cas9UTR_R ...
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bioRxiv - Microbiology 2020Quote: ... The U6 cassette from plasmid pDC_A1G2 was amplified using Q5 high fidelity DNA Polymerase (New England BioLabs; NEB) with primers HD104_Cas9UTR_F and HD104_Cas9UTR_R ...
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bioRxiv - Systems Biology 2021Quote: ... one-cell stage fertilised d/d axolotl eggs were dejellied and injected with 5nl of injection mix (~0.5ng AxFUCCI plasmid and 0.005U I-SceI meganuclease (NEB) diluted in 1X CutSmart buffer (NEB)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were constructed by ligating PCR products amplified with Q5® High-Fidelity DNA polymerase (New England Biolabs) using Golden Gate DNA assembly43 ...
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bioRxiv - Synthetic Biology 2021Quote: All PCR amplifications for plasmid construction and cloning were performed using Q5® High-Fidelity DNA Polymerase (NEB), followed by purification with Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... The amplified product was inserted into pGDB-SDEL plasmid using restriction sites Xho1 and AvrII (New England Biolabs) and transformed into bacteria ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified PCR products were then assembled into intact plasmid using Gibson Assembly Master Mix (NEB, Cat.No. E2611). The newly assembled plasmid was transformed into E.coli Trans5α chemically competent cells (Transgene ...
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bioRxiv - Molecular Biology 2019Quote: ... The hsf1 mutation was generated in the above plasmid by Q5 site-directed mutagenesis (E0552s, New England Biolabs). All single integration plasmids were digested with PmeI before transformation and the correct integrations were confirmed by PCR ...
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bioRxiv - Plant Biology 2019Quote: ... The glgC and gnd knock out plasmids were constructed using HiFi DNA Assembly master mix (NEB, Ipswich, MA). S ...
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bioRxiv - Genetics 2020Quote: ... The purified dephosphorylated linear plasmid and phosphorylated annealed oligos were ligated together using the Quick Ligation Kit (NEB). The ligated product was transformed into One Shot Stbl3 Chemically Competent E ...
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bioRxiv - Cancer Biology 2021Quote: Single gRNA oligonucleotides were cloned into LentiCRISPR 2.1 plasmid (Evers et al. 2016) by BsmBI (New England Biolabs) digestion followed by Gibson Assembly (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmids were either created by molecular restriction cloning or by the Gibson Assembly technique (New England Biolabs, Inc.). HA-PTH1RmTurquoise/mCitrine (PTH1RFRET ...
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bioRxiv - Genomics 2020Quote: ... 1993) and transformation efficiencies were routinely evaluated using a standard batch of the commercial supercoiled plasmid pLITMUS38 (NEB). All E.coli strains used were obtained from New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids (Supplementary Table T 2) were cloned by isothermal assembly using NEBuilder HiFi DNA Assembly Mix (NEB). Plasmids were transformed into E ...
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bioRxiv - Cell Biology 2021Quote: ... the myc tag was removed from the Ccz1-myc plasmid using NEB site-directed mutagenesis (SDM) kit (NEB) following manufacturer’s instructions and primers selected using NEBaseChanger tool (table 1) ...
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bioRxiv - Genomics 2021Quote: ... Resulting supercoiled plasmid is linearized at the 3’ end of the PolyA tail using BamHI-HF (NEB R3136S), and checked for reaction completion by running on agarose gel ...
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bioRxiv - Genetics 2020Quote: ... The mutant Tile 3 plasmid pool was then subcloned into the AttB-KCNH2-HA:IRES:mCherry plasmid by restriction digest with BglII and NdeI (NEB), ligated with T4 ligase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: DNA plasmids were made using conventional molecular biology methods and restriction enzymes (RE; New England BioLabs, Ipswich, MA). An adeno-associated virus serotype 2 (AAV2)-based transfer vector with the human synapsin I promoter (hSyn ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding individual variants were pooled and electroporated into electro-competent NEB10β cells (New England Biolabs, Ipswitch, MA), which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research ...
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bioRxiv - Biochemistry 2022Quote: CSF3R constructs were cloned into an MSCV-IRES-GFP (MIG) plasmid digested with XhoI (New England Biolabs R0146S). For the constructs with truncated domains ...
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bioRxiv - Bioengineering 2022Quote: The plasmid backbone was linearized by sequential digestion with SmaI and SpeI (New England Biolabs, Ipswich, MA, USA), and gel purified from 0.8% SeaPlaque GTG Agarose (Lonza ...
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bioRxiv - Immunology 2022Quote: ... The gBlocks were inserted into a pcDNA 3.1+ Zeocine plasmid using Gibson assembly master mix (New England Biolabs). The cDNA construct encoding LAIR-1 ectodomain (sLAIR-1ecto ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB® 5-alpha was used for plasmid assembly and cloning following the manufacturer’s instructions (NEB). Transformed E ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411 ...