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Citations for New England Biolabs :
1551 - 1600 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and assembled into SalI linearized LentiGuide-GBP-Chr3 sgRNA3 plasmid using HiFi DNA Assembly Kit (New England Biolabs, E5520). Primers used were listed in Supplementary Table S3.
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Molecular Biology 2023Quote: Preparative PCR for plasmid construction were performed with Q5 High-Fidelity DNA Poly-merase (New England Biolabs cat. #M0491S) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... digested plasmids and amplicons were ligated using the T4 DNA Ligase according to the manufacturer’s instructions (New England Biolabs). For Gibson Assembly ...
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bioRxiv - Cancer Biology 2023Quote: ... then ligated into a PB-CRISPRia expression plasmid linearized with BstXI and BlpI (New England Biolabs, Cat. #R0113L, #R0585L). Chemically competent Stable E ...
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bioRxiv - Biochemistry 2022Quote: ... the 6XHis-GB1-Aurora B (59-361 aa) and IN-box (790-844 aa; IN-boxΔC) coding regions were introduced in the pTXB1 plasmid (NEB) by in-fusion cloning using the Nde1 and Sap1 cloning site ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid inserts were amplified by PCR using the Q5® Hot-Start High-Fidelity 2X Master Mix (NEB). Site-directed mutagenesis was performed using the Q5® Site-directed mutagenesis kit (NEB ...
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bioRxiv - Biophysics 2023Quote: ... coli were introduced into the plasmid pRSET-A-FRET (Boersma et al, 2015) via Gibson assembly (New England Biolabs), so the encoded membrane anchor substituted the flexible linker between the mCerulean and mCitrine ...
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bioRxiv - Microbiology 2023Quote: The pGEX-6P-PrkA1-338 plasmid (Table 2) was constructed by a two-part ligation (NEB Quick Ligation kit) of BamHI- and NotI-linearized pGEX-6P and PCR-generated prkA1-338 amplified with primers AS21 and AS81 (Table 3 ...
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bioRxiv - Cell Biology 2023Quote: For protein synthesis the verified plasmid was transformed into BL21 competent cells (C2530, New England BioLabs, Ipswich, MA, USA). Cells were grown shaking at 37°C in 2XYT with appropriate selection to an OD600 of 0.6 and then transferred to 18°C ...
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bioRxiv - Microbiology 2023Quote: ... The complementation plasmid was constructed by Gibson assembly using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
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bioRxiv - Neuroscience 2023Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI and NHE-I (NEB #R3131) cut sites ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified HA-E2F2 fragment was assembled into the linearized FUW-tetO plasmid by Gibson Assembly (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... All expression plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs). All PCR amplified sequences were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: The resulting plasmids were mini-prepped and then digested with PmeI and SapI (R0560, R0569, NewEngland BioLabs, Waltham, MA) to generate a 6.01kb fragment containing the Whi3 coding sequence and 5’ UTR ...
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bioRxiv - Biochemistry 2023Quote: ... Both the δ insert and pET28 plasmid were digested with the restriction enzymes XbaI and BamHI (New England Biolabs) and then purified by gel extraction using the QIAquick Gel Extraction kit (Qiagen) ...
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bioRxiv - Biochemistry 2023Quote: ... were generated using the vPRC1.4 recombined plasmid (containing all three expression cassettes) as template and using Phusion polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: A 10 µL reaction containing 200 ng of pTrc200HA plasmid DNA and 1× CutSmart Buffer (New England BioLabs, USA) with partially purified V2c or its variants normalized by the major V2c protein band was incubated at 37°C for 1 hour ...
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bioRxiv - Systems Biology 2023Quote: ... originating from plasmid pGD009 20 was ligated into the backbone using NEB T4 Ligase (New England Biolabs, Ipswitch, MA) according to the manufacturers protocol using 29 fmol of backbone and 87 fmol of insert ...
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bioRxiv - Genetics 2024Quote: ... DNA template was prepared by cloning into a pUC19 backbone and plasmid was linearized using SapI restriction enzyme (NEB) and purified using DNA Clean & Concentrate (Zymogen) ...
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bioRxiv - Biochemistry 2023Quote: ... using the plasmid pSP73-JY0 as template (55) followed by digestion with the restriction enzyme MluI (New England Biolabs). Labelled handles were ligated with the central part with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and TurboID from Dr. Alice Ting’s plasmid (Tess C. Branon et al., 2018) via PCR using Q5 high-fidelity (M0492, NEB). Next ...
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bioRxiv - Synthetic Biology 2024Quote: The plasmid DNA was subjected to double digestion using KpnI and XbaI enzymes (New England Biolabs, Ipswich, MA, USA), followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA with similar profiles was additionally differentiated by digestion with the BamHI and HindIII restriction enzymes (NEB, USA). The digestion was performed according to the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2023Quote: ... mixed in a ratio 1:3 (insert:backbone plasmid) and ligated with T4 ligase according to manufacturer’s instructions (Quick Ligation kit, NEB M2200). The ligation reaction was transformed into competent TOP-10 bacteria and plated on agarose plates with Ampicillin ...
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bioRxiv - Biophysics 2023Quote: ... PhoCl-2c and TMEM115 were cloned into the pTREtight2-vector plasmid using NEBuilder HiFi DNA Assembly (New England Biolabs). The FLAG-BK-YFP fragment was amplified from FLAG-BK667YFP plasmid27 ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid pEC095 was digested with BtsBI and the 5’ overhanging DNA ends were filled in with T4 Polymerase (NEB). A kanamycin resistance marker ...
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bioRxiv - Molecular Biology 2024Quote: ... and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.). The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... Plasmid pools were digested with SphI-HF and SpeI-HF and treated with Antarctic phosphatase or Quick CIP (NEB), then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase) ...
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bioRxiv - Biophysics 2024Quote: ... pCAGEN_6×His-DNAJA2-HA was created from PCR of the ORF from pColdI_6×His-DNAJA2 and insertion into EcoRI-digested pCAGEN plasmids using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was then used to produce pCAGEN_3xFLAG-DNAJA2 in a similar manner ...
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bioRxiv - Genomics 2019Quote: ... mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA, USA). E14 genomic DNA was extracted with a DNeasy Blood and Tissue Kit (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... or anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) were used as the secondary antibodies.
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Developmental Biology 2023Quote: Regulatory elements were amplified from mouse genomic DNA with Q5 polymerase (NEB, M0491) using primers listed in Table S8 and cloned into pGL4.24[luc2P/minP] (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: ... and +1 sites of the promoters controlling transcription of both RNAs were used to amplify the standard pUC19 plasmid (NEB). After PCR amplification samples were digested with DpnI (NEB ...
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bioRxiv - Biophysics 2021Quote: The DNA substrate used in the optical trapping assay was a ~3400-bp PCR-amplified section of plasmid PBR322 (New England Biolabs). The forward primer (5’-/btn/-ACA GCA TCG CCA GTC ACT AT-3’ ...
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bioRxiv - Biophysics 2021Quote: The assembly of GRB2 SH3 and PSD95 PDZ mutant libraries in both assay plasmids were done overnight by temperature-cycle ligation using T4 ligase (New England Biolabs) according to the manufacturer's protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The sgRNA expression plasmids were cloned by insert annealed oligos into the pU6-sgRNA vector that was digested by Bbs I (NEB). shRNA for LacZ (negative control ...
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bioRxiv - Cell Biology 2020Quote: ... All other plasmids were constructed using classical subcloning or Gibson assembly using Hot Start High Fidelity Q5 polymerase from NEB. All constructs generated and used in this study were verified with sequencing of the coding region of the plasmids.
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bioRxiv - Cell Biology 2020Quote: ... photoactivatable-mCherry was PCR-amplified from the plasmid N-PA-mCh and assembled into the retroviral vector pBABE-puro using HiFi DNA Assembly (E5520S, NEB). To create the stable U2OS-dCas9-PA-mCh/GFP-Parkin and U2OS-dCas9-PA-mCh/TFEB-GFP cell lines ...
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bioRxiv - Cell Biology 2020Quote: ... The amplicons were cloned into PCR-linearized pPR3-N prey plasmid (Dualsystems Biotech) at the SfiI sites using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs), and were transformed into DH5α competent cells and plated onto 48-well Bioassay Qtrays (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... carrying the 20mer guide sequence of GAL1 were used to PCR-amplify the plasmid pML104 (Laughery et al., 2015) using the Q5 site-directed mutagenesis protocol (New England Biolabs) as described by (Hu et al. ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids(von Boehmer et al., 2016) using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... These gBlocks were assembled into an expression vector on the pETDuet-1 plasmid backbone with the help of the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). First ...
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bioRxiv - Developmental Biology 2021Quote: ... Positive clones were digested with the appropriate enzyme to linearize the plasmid and anti-sense ribonucleoprobe synthesis was carried out using Sp6 or T7 RNA polymerase (New England Biolabs)+DIG labeled UTP (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... Barhl1a riboprobe was synthesized as previously reporter 19 and gfp riboprobe was synthesized from a linearized pCS2:GFP plasmid with NotI (Fermentas or New England Biolabs) and transcribed with Sp6 (mMessage mMachine Sp6 ...
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bioRxiv - Developmental Biology 2020Quote: ... 3 fragments have been inserted into pCS2+ plasmid linearized with Xho1 using the Gibson Assembly Cloning Kit (New England Biolabs): a first fragment of 4161bp of the ancBE4max to the PIM domain (amplified using the primers F-5’-CGATTCGAATTCAAGGCCTCATGAAACGGACAGCCGAC-3’ and R-5’-CGGTCTGGATCTCGGTCTTTTTCACGATATTC-3’) ...
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bioRxiv - Cell Biology 2022Quote: ... human emerin was first fused to the C-terminus of a SNAP tag by AscI and XhoI insertion in a pSNAP-tag(m) plasmid (NEB). SNAP-emerin was then subcloned into a modified pFUW lentiviral vector by NheI and AgeI insertion ...