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Citations for New England Biolabs :
1401 - 1450 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The plasmids for this work were constructed using the Gibson-Assembly technique (NEBuilder protocol, New England Biolabs, Germany). Plasmid constructions were first established in E ...
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bioRxiv - Biochemistry 2023Quote: Site directed mutagenesis for I72W M.TaqI mutation on M.TaqI plasmid a was carried out using Q5 Site-Directed Mutagenesis Kit (NEB) by following kit protocol and expressed ...
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bioRxiv - Microbiology 2022Quote: ... Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutagenesis of the HA-AHR plasmid was performed using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S).
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bioRxiv - Biophysics 2023Quote: ... the CBP-TEV sites was removed from plasmid pRS306 orc1-gal1-10-orc2 through Gibson assembly (NEB #E2611L) using primers TL-441 ...
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bioRxiv - Microbiology 2023Quote: ... Constructs for plasmid-based expression of genes were generated using PCR and Gibson Assembly® (NEB, Boston, MA) followed by cloning into pMQ72 ...
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bioRxiv - Synthetic Biology 2023Quote: ... All plasmids were generated using standard cloning procedures and the NEBuilder HiFi DNA assembly system (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described 40 ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA containing one copy of the SIV gene was linearized using EcoR1 (New England Biolabs, Ipswich, MA) following their restriction digest protocol (New England BioLabs ...
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bioRxiv - Biochemistry 2023Quote: ... All DNA fragments were fused in pCR2.1-TOPO (genome integration cassette) or pGRB2.0 (rescue plasmids) vectors using a Gibson assembly kit (New England Biolabs) and validated by sequencing ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs Cat. #E2621) and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat ...
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bioRxiv - Developmental Biology 2023Quote: Plasmids (otxG:Tet-ON 3G; TRE3Gs:Cas9; TRE3Gs:Kaede; U6:sg2; Table S1) were built by Gibson cloning (NEB, Cat# E2611) following standard protocols ...
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bioRxiv - Genetics 2023Quote: ... All PCR amplifications for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmids were constructed in the present using NEB-HiFi DNA assembly master mix from NEB (Beverly, MA).
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bioRxiv - Synthetic Biology 2023Quote: The two plasmids pEKEx2_low_mCherry and pEKEx2_high_mCherry were assembled via Gibson cloning using the Gibson Assembly® Master Mix (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... was subcloned into the pIRES2-dsRED2 plasmid using PCR with ag409 and ag410 and NotI restriction digestion (NEB) to create KCNE1-HA:pIRES2:dsRED2 ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
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bioRxiv - Biochemistry 2024Quote: Plasmid constructs were cloned by Gibson assembly(51) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Codon-optimised gene fragments for wild-type MxEnc and the triple mutants 3×His-MxEnc ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB #M0530L), restriction enzymes (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... plasmid backbones without eYFP were amplified by PCR with PR41 and PR42 using Q5 Polymerase (New England Biolabs) and pAJM657 ...
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bioRxiv - Microbiology 2023Quote: ... the pBOMBL12CRia(ct226) plasmid was modified by SalI-digest and insertion of a gBlock (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Molecular Biology 2023Quote: ... The circularization of the plasmids was then achieved using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520). The primers utilized for site-specific mutagenesis are presented in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... the pBOMBL12CRia(ct226) plasmid was modified by SalI-digest and insertion of a gBlock (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Microbiology 2023Quote: Plasmid mAID-5xTy/DHFR/OsTir1_N was constructed by Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix; NEB) of the following fragments ...
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bioRxiv - Biophysics 2023Quote: A Tandem HaloTag plasmid containing the HaloTag-GTGSGSGS-HaloTag cDNA sequence was built via Gibson Assembly (NEB, (62)) of two HaloTag genes ...
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bioRxiv - Genetics 2023Quote: ... The ints-4 sgRNA was inserted into plasmid pDD162 via the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). The sgRNA plasmid and repair template were Sanger sequenced to confirm correct construct assembly followed by microinjection into EG9882 strain with integrated Cas9 activity (10 ng µl-1 repair template ...
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bioRxiv - Molecular Biology 2023Quote: ... the positive control and each viral mutant plasmid was constructed using the Q5 site-directed mutagenesis kit (NEB) according to manufacturer protocol and verified via whole plasmid sequencing ...
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bioRxiv - Biochemistry 2023Quote: 16.5 nM of linearized template plasmid was added to IVT reactions in RNA polymerase buffer (New England Biolabs) containing 5 mM of each rNTPs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... To confirm integration of the ampicillin marker plasmid were digested with ScaI and XbaI (NEB R3122L and R0145L) and verified using gel electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... The plasmids harboring the desired insertions (UPR TF coding sequences) were linearized by incubation with restriction enzymes (NEB). Linearized plasmids were subjected to invitro transcription using a SP6 Transcription Kit (Invitrogen AM1340) ...
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bioRxiv - Microbiology 2023Quote: ... plasmid was pre-treated with Alkaline phosphatase and amplicons were treated with T4 polynucleotide kinase (New England Biolabs). Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Genetics 2023Quote: All plasmids were generated using Gibson Assembly strategies67 using NEBuilder® HiFi DNA Assembly Master Mix (NEB #E2621) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Linearization of the homology donor and pKS diaCas9_sgRNA plasmids were performed by KpnI-HF® and NdeI (NEB) restriction enzymes respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR amplifications for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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bioRxiv - Immunology 2024Quote: ... we found cloning was most efficient when we digested the library and plasmid with BsmBI-v2 (NEB, #R0739L) (but not the barcode fragment due to the quantity ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR amplification for plasmid and vector constructions were performed using Q5 polymerase (New England Biolabs, Ipswich, MA). Most PCR reactions were performed using the following conditions ...
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bioRxiv - Bioengineering 2024Quote: ... The pTA-Mob plasmid (gift from Thomas Hamilton and David Edgell, Western University) was linearized using AvrII (NEB) and combined with the amplified fragment from pFAST-PETase-cis using a modified NEB Hifi Assembly protocol with a two-hour assembly step at 50 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Guide RNA spacers were cloned into rcRNA vectors by digesting plasmids with Esp3i restriction enzyme (New England Biolabs) for 1 hour at 37°C according to manufacturer’s directions ...
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bioRxiv - Developmental Biology 2024Quote: ... We verified that this guide RNA digested the Gbait plasmid in combination with Lba Cas12a (New England Biolabs) in an in vitro reaction (data not shown) ...
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bioRxiv - Cell Biology 2023Quote: All plasmids used in this study were constructed using the NEBulider (New England Biolabs, Ipswich, USA; Cat #E2621L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The linearized plasmid library and barcoding oligo were assembled with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) at a 1:5 molar ratio ...
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid libraries were first double-digested with PmlI and MfeI-HF restriction enzymes (New England Biolabs, Ipswich, MA), then purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Genetics 2021Quote: ... The synthetic oligo was cloned into the plasmid backbone by using the Gibson assembly kit (NEB, Gibson Assembly®) and the ligation reaction was carried out for 1 hour at 50 °C ...
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bioRxiv - Microbiology 2019Quote: ... The original plasmid template was then digested using the restriction enzyme DpnI in Cutsmart buffer (1X) (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Microbiology 2022Quote: ... we first generated two plasmids encoding guide RNAs targeting either MMCAP2_0582 or MMCAP2_0575 by modification of the base plasmid p426-SNR52p-gRNA.Y-SUP4t (25) using the Q5 Site-Directed Mutagenesis kit (NEB). In addition ...
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bioRxiv - Neuroscience 2021Quote: RNAs were in vitro transcribed from PspOMI-linearized pcDNA3.1(+) reporter plasmids using HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA ...