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Citations for New England Biolabs :
1251 - 1300 of 2483 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... the 10XUAS-DSCP promoter was amplified from plasmid pNP with Q5 High-Fidelity DNA Polymerase (NEB) using primers UP0394 and UP0395 ...
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bioRxiv - Neuroscience 2023Quote: ... Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs, Ipswich, MA), [α-35S]-CTP ...
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bioRxiv - Synthetic Biology 2023Quote: All PCR reactions used for plasmid assembly were done using the 2x Phusion mix from NEB, in a 50 µL volume ...
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bioRxiv - Developmental Biology 2023Quote: ... The amplicon was subcloned into pN2-CMV:RFP plasmid using XhoI/EcoRI restriction enzyme digestion-ligation (NEB) to generate pN2-CMV:AmRps14-RFP plasmids and sequence verified ...
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bioRxiv - Immunology 2023Quote: ... All fragments were assembled into a pLIX plasmid linearized by NheI and SalI (New England Biolabs) using HiFi DNA Assembly Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... digested plasmid and amplicon were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: DNA templates for the transformation assays were prepared by PCR amplification from the pUC19 plasmid (NEB), after which the PCR fragments were Dpn1-treated ...
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bioRxiv - Biochemistry 2023Quote: PCR mutagenesis was performed by whole plasmid PCR with Q5 high-fidelity polymerase (New England Biolabs) followed by PCR purification (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: All plasmids were generated by using some combination of PCR amplification using Q5 DNA polymerase (NEB), HiFi Assembly (NEB ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were assembled via Gibson assembly using the HiFi DNA Assembly mix (New England Biolabs), unless stated otherwise ...
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bioRxiv - Cell Biology 2023Quote: ... The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs) and treated with a Quick Blunting Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were used for cloning plasmids smaller than 10 kb and NEBStable (NEB cat no. C3040I) competent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid containing the origin DNA sequence was digested with HindIII and EcoRI (New England Biolabs) according to manufacturer’s protocol (Supplemental Figure S2A ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid constructs were created by Gibson cloning with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L) or Gibson Assembly Master Mix (NEB E2611L) ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... small insertions or mutations were inserted into plasmids using Q5 site-directed mutagenesis (NEB CN# E0554S) according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Template plasmid was then removed by addition of 1 μL DpnI (New England BioLabs, Cat.#R0176), 5 μL 10X FastDigest Buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... FlucWT-HA-GFP11 and FlucDM-HA-GFP11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP11 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the pAY24 plasmid digested with PstI and SmaI (New England BioLabs, #R0140 and #R0141, respectively). The sequence of all constructs was confirmed by Sanger sequencing at the Genomic Core Facility.
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Bioengineering 2023Quote: ... All plasmid construction was done by Gibson Assembly (HiFi DNA Assembly Master Mix, New England Biolabs) and validated by colony PCR and sequencing ...
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bioRxiv - Cell Biology 2024Quote: Plasmid with the requisite gene sequence was linearized using not1 or hind III (New England Biolabs) restriction enzyme and was used as template ...
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bioRxiv - Cell Biology 2024Quote: ... Double digested PCR product and plasmid were further subjected to Ligation using T4 DNA ligase (NEB) at 16°C for 12-14 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The pPbU6-hdhfr/yfcu plasmid and the synthetic fragment were digested with BsmBI and AatII (NEB) and ligated into the vector with T4 ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... We digested pWallium20 backbone plasmid with the restriction enzymes HpaI and PstI (NEB # R0105S and R0140T) followed by gel purification ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Level 0 plasmid was amplified with the primer pair SLo0765/SLo0766 followed by DpnI (NEB) digestion prior to ligation (any Level 0 plasmid will do ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Microbiology 2020Quote: ... The cut plasmid and PCR product were then purified and ligated by T4 ligation (New England Biolabs). For constructing the pZKF2 (pBBR-ftsZGFP) ...
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bioRxiv - Cell Biology 2020Quote: ... and the point mutation plasmids generated using Q5 site-directed mutagenesis (New England Biolabs, Beverly, MA, USA). For lentiviral transfection ...
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bioRxiv - Cell Biology 2020Quote: All the plasmids generated for the opto-release system were cloned using the Gibson Assembly System (NEB) by combining pRK5.1 backbone with desired PCR fragments according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The size-selected fragment was cloned into the digested plasmid with NEBuilder HiFi DNA Assembly (NEB E2621), and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K) ...
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bioRxiv - Molecular Biology 2022Quote: ... the constructed plasmids were transformed by heat shock into chemically competent Top10 Escherichia coli (New England BioLabs). Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.0001ng of pUC19 plasmid methylated at 100% of CpG sites (New England BioLabs, Ipswich, MA, USA) were used as spike-in controls to determine the efficiency of APOBEC deamination and TET2 oxidation ...
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bioRxiv - Molecular Biology 2020Quote: ... pGEMHE plasmids were linearized and 5’-capped mRNA was synthesised with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and cloned into the pHERD30T-IC plasmid to replace araC-ParaBAD using Gibson Assembly (New England Biolabs). Without induction ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the plasmid pSC201 (cut with XbalI and SphI) in a Gibson-assembly mix (NEB). The reaction was transformed into E ...
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bioRxiv - Cell Biology 2019Quote: ... and 1B11 DNA and 10ng of pGRP170-DDD plasmid was digested overnight with Mfe1 (New England Biolabs). The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The gltA complement plasmid was constructed using a Gibson Assembly Cloning Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Mini-CRISPR elements and plasmid pAY5211 were digested using the restriction enzymes KpnI and BamHI (NEB, USA) and ligated using the Quick Ligation Kit (NEB ...
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bioRxiv - Immunology 2020Quote: ... Plasmid digestions were performed following manufacturer recommendations and generally subjected to Antarctic phosphatase (New England Biolabs # M0289S) treatment to prevent potential re-annealing ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed on a plasmid template using the Q5 High-fidelity 2× Master Mix (NEB, M0492L), with primers at final concentration of 500nM ...