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Citations for New England Biolabs :
1301 - 1350 of 2483 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... The protospacer-containing plasmids were further modified by excising the Cas9 ORF with PstI (New England Biolabs). Each final plasmid contains the U6 promoter ...
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bioRxiv - Genetics 2020Quote: ... We mixed 20 µg of insert or 15 µg plasmid library with 5 µL AscI (NEB R0558L), 5 µL SbfI-HF (NEB R3642L) ...
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bioRxiv - Microbiology 2021Quote: ... The isolated plasmid was then purified (linear and open circular forms digested) via T5 exonuclease (NEB: M0363) reaction and the NucleoSpin Gel and PCR Clean-up kit (Machery-Nagel) ...
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bioRxiv - Bioengineering 2020Quote: GFP1-10 was inserted into the pBAD plasmid via NEBuilder® HiFi DNA Assembly (New England Biolabs): 25 ng of the digested vector ...
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bioRxiv - Bioengineering 2021Quote: ... were PCR-amplified and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was isolated using NucleoBond miniprep spin columns and then digested with EcoRI (New England Biolabs). Three clones with a wildtype restriction pattern were sequenced in both directions with the M13F and M13R primers by the Northwestern University sequencing core (NU-Seq).
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was digested for 1 hour at 37°C with NcoI (New England BioLabs, Ipswich, MA) unless otherwise specified in a final volume of 20 μl before evaluating restriction fragment patterns by 1% agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids harbouring MICAL1 domain mutants were generated using Q5® Site-Directed Mutagenesis Kits (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Carrier plasmid and the PCR product from the last step were digested with BsmBI (NEB, buffer 3.1) at 55°C for 1h and were purified for ligation ...
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bioRxiv - Immunology 2021Quote: ... The kRSV-DB1-QUAD plasmid and spike insert were digested with the enzymes AatII and SalI (NEB) and ligated with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... or the same plasmid digested overnight with HindIII-HF and SbfI-HF (New England Biolabs, Ipswich, Massachusetts) were digested overnight with 10 units of Plasmid-Safe ATP-Dependent DNAse (Lucigen ...
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bioRxiv - Microbiology 2021Quote: ... 12.5μL of plasmid is mixed with 1.5μl of Custmart buffer and 1μl of I-SceI (New England Biolabs), and incubated at room temperature for 5 min before being put on ice until injection of 1 nL inside the cell of AB embryos at the one-cell stage.
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bioRxiv - Microbiology 2021Quote: ... Annealed oligos were gel-purified from 2% agarose gel and T7_2xS1m_SacI_BamHI DNA fragment was ligated with SacI+BamHI-digested pUC19 plasmid using T4 DNA ligase (NEB). The resulted plasmid was digested with BamHI and XbaI and ligated with 2xS1m_BamHI_XbaI dsDNA oligo to generate pUC19-T7-4xS1m construct ...
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bioRxiv - Molecular Biology 2020Quote: ... The mutant env gene was amplified from pTZ57/R-env plasmid pools using Phusion DNA Polymerase (NEB) and primers from 5’ and 3’ ends ...
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bioRxiv - Molecular Biology 2021Quote: pACYC184Δtet5’ DNA extraction for supercoiling analysis was performed by using the Monarch®Plasmid Miniprep Kit (NEB). Cells were grown at the indicated temperature to an OD600 of 0.4 ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pSF741 was used in a HiScribe T7 in vitro transcription kit (New England Biolabs, Ipswich, MA) to generate Venus mRNA ...
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bioRxiv - Biophysics 2020Quote: ... we used an alternative strategy that enables us to propagate expression plasmids in standard bacterial strains (NEB 5-alpha Competent E ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence intensity measurements of SYBR Gold are recorded in the presence of linear pBR322 plasmid DNA (NEB), which is produced by restriction of supercoiled circular pBR322 using restriction enzyme EcoRV (NEB ...
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bioRxiv - Systems Biology 2022Quote: ... Before electrophoresis, extracted plasmids were mixed with Purple Gel Loading Dye (6X, no SDS) (New England Biolabs) and loaded into wells in the agarose gel ...
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bioRxiv - Molecular Biology 2022Quote: ... all plasmid templates were linearized with XbaI and were in vitro transcribed with T7 RNA polymerase (NEB). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... protegens CHA0 and cloned in both pKT25 and pUT18C plasmids using BamHI and EcoRI (New England Biolabs). The integrity of the cloned sequences was verified by Sanger sequencing (Microsynth) ...
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bioRxiv - Biochemistry 2022Quote: ... Cleavage reactions were initiated by the addition of 34.82 fmol of linearized plasmid (digested with HindIII (NEB)) and buffer to bring the total reaction volume to 22.5 μL with a final composition of 10 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Freshly prepared supercoiled plasmid DNA was then isolated from overnight cultures using a Monarch miniprep kit (NEB) and then immediately subjected to restriction digestion with T7 endonuclease (NEB ...
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bioRxiv - Microbiology 2020Quote: ... The amplicon was cloned in the pXDC39 plasmid vector using the restriction enzymes SacI (New England Biolabs) and XbaI (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... the genomic clone was released from the bacterial plasmid by digestion with Pac I (New England Biolabs), and 1 μg of linearized DNA was transfected into A549 cells using Effectene Transfection Reagent (Qiagen ...
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bioRxiv - Microbiology 2020Quote: The pcDNA3.1(-)-sACE2 plasmid expressing soluble ACE2 was made by Q5 site-directed mutagenesis (New England Biolabs) from the pcDNA3.1(-)-ACE2-Strep plasmid (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... and the PCR fragment of the plasmid were assembled together using the Hifi DNA assembly mix (NEB). The construction was verified by sequencing.
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bioRxiv - Microbiology 2021Quote: ... The parA fragment and the two plasmid fragments were assembled using the Hifi DNA assembly mix (NEB). The construction was verified by sequencing.
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bioRxiv - Microbiology 2021Quote: ... The oligos were then ligated into the double digested psiCheck-2 plasmid using T4 DNA ligase (NEB) at room temperature for 2 h ...
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bioRxiv - Biophysics 2021Quote: ... We excised the luciferase gene and linearized the pLEW100V5 plasmid with HindIII (R3104S, New England Biolabs, MA) and BamHI (R3136S ...
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bioRxiv - Immunology 2022Quote: ... All plasmids were transformed into high efficiency chemically competent cells DH5α (New England BioLabs, Ipswich, MA, USA) using the heat shock method in a water bath at 42°C for 30 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... and RPAS384A mutants were generated in this plasmid background using Q5 site-directed mutagenesis (New England Biolabs). RPA ...
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bioRxiv - Molecular Biology 2019Quote: ... the integrative plasmid pJRL2-pBMH1-yEGFP containing homologies to LEU2 was cut by AscI (New England Biolabs) and transformed ...
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bioRxiv - Microbiology 2019Quote: ... the product was cloned into the pET1-5b N-terminal 6×His expression plasmid (New England Biolabs).
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bioRxiv - Microbiology 2019Quote: ... PCR products and expression plasmid pJN105 (Newman and Fuqua, 1999) were digested with EcoRI and SacI (NEB) for 37°C for 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... The 176 bp GUB DNA was cut out from the purified plasmid with EcoRV (New England Biolabs). The plasmid backbone was removed by polyethylene glycol precipitation using PEG-6000 ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products used for sequencing or plasmid synthesis were performed with Q5 high-fidelity polymerase (NEB). The resulting DNA fragment was digested and ligated into NdeI-xhoI digested pET21b(- ...
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bioRxiv - Molecular Biology 2021Quote: ... The pBluescript plasmid was cut by EcoRV and dephosphorylated by Calf Intestinal Alkaline Phosphatase (CIP) (NEB, M0290L), then ligated with the phosphorylated PCR fragments by T4 DNA Ligase (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone and were designed to overexpress recombinant proteins as C-terminus His-tagged fusions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were built by Gibson Assembly using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs, # E2621). After assembling ...
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bioRxiv - Microbiology 2020Quote: Fragment amplification for constructing remaining plasmids was performed using Q5 high-fidelity DNA polymerase (NEB, Cat. # M0491). A TRL2(Δgfp)-NH fragment was amplified using pTRL2-NH-GrgA as template ...
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bioRxiv - Microbiology 2021Quote: ... Site directed mutagenesis of plasmids were performed using Q5® Site-Directed Mutagenesis Kit (New England BioLabs) following manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... The pET28b plasmid and the PCR amplified target sequences were double digested using SalI-HF (NEB, # R3138S) and XbaI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µL of the purified product was ligated into a circular plasmid using KLD reaction mix (NEB) incubated for 10 min at room temperature.
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bioRxiv - Microbiology 2021Quote: ... Plasmid assembly was performed using Gibson assembly and the NEB HiFi assembly kit (NEB, New England, MA). The assembly mixture was transformed into E ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding GlxChiB and its mutants were transformed into E.coli SHuffle T7 (DE3) cells (New England Biolabs). Cells harboring the plasmids were cultured at 37 °C in LB medium containing 100 mg/L ampicillin-Na for about 3.5 hours to reach OD600 of 0.6-0.8 and then were induced to express recombinant protein by adding IPTG to a final concentration of 0.1 mM ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR fragment and the plasmid (pOPINS3C) were assembled using the NEBuilder HiFi DNA Assembly kit (NEB), following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: The full-length PARP1 gene was purchased from GE Healthcare and subcloned into a pACEBac1 plasmid bearing an N-terminal 6xHis-tag via a Gibson Assembly (NEB). The PARP2 expression plasmid (C-terminal FLAG-6xHis-tag ...
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bioRxiv - Synthetic Biology 2021Quote: The CFP and GFP reporter plasmids were propagated in either dam-/dcm-strain C2925 (New England Biolabs) or dam+/dcm+ strain DH5-alpha Turbo (NEB C2984 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid DE was assembled via Gibson assembly (Gibson Assembly Master Mix of New England BioLabs, Inc.). The assembly was performed at equimolar concentrations of linearized plasmid MinDpUC57 and MinE DNA fragments for 1 hour at 50 °C ...