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Citations for New England Biolabs :
1601 - 1650 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... was amplified from the LRP1 plasmid15 and inserted into the TOPO genomic sequence plasmid to replace the stop codon using NEBuilder HiFi DNA assembly master mix (New England Biolabs). CRIPSR guide RNA (gRNA ...
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bioRxiv - Molecular Biology 2021Quote: The singly nicked pCG09 plasmid was prepared by incubating CsCl purified negatively supercoiled pCG09 (60 μ l of 1X NEB Smart Cut buffer with the nicking endonuclease Nb.BbVCI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Gluc200 and Gluc200A44 templates were generated using PCR amplification of GLuc of the first 200 nt at the 5’end of pCMV-GLuc 2 Control Plasmid (NEB: https://www.neb.com/tools-and-resources/interactive-tools/dna-sequences-and-maps-tool) ...
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bioRxiv - Biophysics 2022Quote: ... was performed using plasmid pHSCRP-His6-H17C-C178S [constructed from plasmid pAKCRP-His6 (16) using site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB)].
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bioRxiv - Biophysics 2022Quote: ... single point mutations were introduced to the respective wild-type plasmids by site-directed mutagenesis using the Q5 site-directed mutagenesis Kit (NEB). The used primers are listed in table 1.
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bioRxiv - Genomics 2020Quote: ... 180 ng control linearized plasmid DNA and 180 ng of 3C library was treated with either 0.5 Unit exonuclease V (NEB, M0345) or 0.5 Unit T5 exonuclease (NEB ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions were performed in 50 μl volumes with 1 ng pFA-MNase plasmid and the Q5 high fidelity polymerase (New England Biolabs). PCR thermocycling was executed as following ...
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bioRxiv - Molecular Biology 2019Quote: Overlapping primers for each cbf5 mutation were used to introduce mutations in a pUC19-ScCbf5 plasmid by QuikChange mutagenesis using Q5 DNA polymerase (NEB) (Table 1) ...
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bioRxiv - Developmental Biology 2019Quote: ... and Tol2-elavl3:mCherry-CAAX was assembled from the digested plasmid and PCR products using Gibson Assembly Master Mix (NEB). The insert was sequenced to confirm ligation fidelity.
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bioRxiv - Synthetic Biology 2019Quote: ... By mixing the linearized pCRISPR-BEST plasmid and chemically synthesized spacer containing oligo with the NEBuilder (New England Biolabs, USA). The linearized pCRISPR-BEST plasmid then will be bridged by the spacer containing oligo ...
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bioRxiv - Biochemistry 2020Quote: Site-directed mutagenesis was carried out by PCR amplification of the starting plasmid with forward and reverse mutagenesis primers containing the desired mutation (Table 1) followed by DpnI (NEB) treatment ...
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bioRxiv - Genomics 2019Quote: ... and then ligated into PiggyBac plasmid pB-TRE3G-BsmBI-EF1α-HygroR-P2A-rtTA by Golden Gate Assembly with enzyme BsmBI and T4 ligase (NEB).
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bioRxiv - Genomics 2019Quote: ... and then ligated into PiggyBac plasmid pB-TRE3G-BsmBI-EF1α-PuroR-P2A-rtTA by Golden Gate Assembly with enzyme BsmBI and T4 ligase (NEB). The ScFV-sfGFP-GB1-NLSSV40 fragment was amplified by PCR from plasmid pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE (Addgene Plasmid # 60906 ...
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bioRxiv - Cell Biology 2019Quote: ... This was sub-cloned into a (pUAS-k10.attB) plasmid using standard restriction digestion with NotI and BamHI (New England Biolabs) followed by ligation with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was circularized again by incubating the phosphorylated product with 400 U of T4 DNA ligase (New England Biolabs) for 2 hours at 16°C ...
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bioRxiv - Cell Biology 2019Quote: ... The 3xHA epitope was PCR-amplified from a pFA6a-3xHA-his3MX6 (Longtine et al. 1998) plasmid using Q5 DNA polymerase (New England BioLabs) and assembled into pFA6a-GFP-hisMX6 (Longtine et al ...
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bioRxiv - Molecular Biology 2019Quote: ... that were integrated into various backbone plasmids (e.g. p28-GFP, p28-HA, or TCV_sg2R) using Gibson Assembly cloning (NEB, Ipswich, MA). The identities of all new constructs were verified with Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... and the CMV promoter was removed to prevent aberrant transcription by digesting the plasmid with ClaI-HF and BamHI-HF (NEB), gel extracting ...
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bioRxiv - Bioengineering 2019Quote: ... the 720 bp Cerulean cassette between the AgeI/HpaI sites of pPPIA-Cer-Fib was replaced with a 560 bp SNAP tag fragment PCR amplified from plasmid pSNAPf (New England Biolabs) using primer pair Snap-XmaI-For/Snap-HpaI-Fib-Rev and double digested with XmaI/HpaI ...
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bioRxiv - Biochemistry 2019Quote: The required coding region was amplified from the H6-TtBac construct by PCR and was cloned into the plasmid pHis17 using Gibson Assembly (New England Biolabs), resulting in a C-termin tag ...
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bioRxiv - Biochemistry 2019Quote: The required coding region was amplified from the H6-TtBac construct by PCR and was cloned into the plasmid pHis17 using Gibson Assembly (New England Biolabs), The plasmid was used to transform C41(DE3 ...
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bioRxiv - Genomics 2020Quote: ... The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB) for 1 h at 50 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and then mixed the plasmids with gBlocks in a 1:3 molar ratio in the presence of NEBbuilder HiFi DNA (New England Biolabs) assembly mix according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Integration into the yeast genome via homologous recombination at the URA3 or LEU2 locus was achieved by transformation of linearized plasmids (NotI digestion, NEB) whereas replicating CEN6/ARS4 or 2µ plasmids were transformed directly into yeast without pre-digestion with NotI ...
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bioRxiv - Cell Biology 2019Quote: ... The MADBUB plasmid was generated using Gibson cloning using the NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Gibson assembly mixes were used according to manufacturer instructions to construct the plasmids used in this study (New England Biolabs). All recombinant strains and constructs used in the study were tested by colony PCR and verified for accuracy by Sanger sequencing.
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bioRxiv - Cell Biology 2019Quote: ... All the OptoSrc and mutants plasmid construction were cloned in a Nhe1-Not1 digested pSico backbone amplified by PHUSION high fidelity DNA polymerase (NEB) using Gibson assembly (NEB ...
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bioRxiv - Bioengineering 2019Quote: ... The pgRNAMPN142-400 plasmid is constructed by removing the gRNAMPN372 from the pgRNA-triple-target plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Genomics 2019Quote: ... Site-directed mutagenesis for Exd and Hth was performed via amplification of the original plasmid with primers harboring single amino acid replacements (arginine to alanine) using Taq-polymerase (NEB). Double and triple mutations were generated consecutively ...
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bioRxiv - Cell Biology 2019Quote: ... were PCR amplified using the primers pAVA0421-AAP4-FR and cloned into the pAVA0421 plasmid (Alexandrov et al., 2004) by Gibson Assembly (NEB). The fusion protein was expressed in BL21 Codon Plus (DE3 ...
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bioRxiv - Biochemistry 2021Quote: Sequence verified effector constructs (∼100 ng plasmid DNA) were chemically transformed into SHuffle T7 Express C3029 (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: Sequence verified effector constructs (∼100 ng plasmid DNA) were chemically transformed into SHuffle T7 Express C3029 (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR product and the plasmid pT1-3B (42) was digested with the restriction enzymes Sal1 and HindIII (New England Biolab (NEB)) ...
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bioRxiv - Biophysics 2021Quote: Wild-type MukB was 6×His-tagged at the C-terminus and was expressed from plasmid Pet21 in C3013I cells (NEB). For Immobilized His6-tagged MukB ...
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bioRxiv - Microbiology 2021Quote: The resulting fragment was ligated into VSV NG-P (Jia et al., 2020) plasmid linearized with MluI and NotI using T4 ligase (NEB).
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bioRxiv - Microbiology 2021Quote: ... The NdeI-HindIII-cut pET21b plasmid backbone and parB* gBlocks fragments were assembled together using a 2x Gibson master mix (NEB). Gibson assembly was possible owing to a 23-bp sequence shared between the NdeI-HindIII-cut pET21b backbone and the gBlocks fragment ...
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bioRxiv - Evolutionary Biology 2020Quote: The transgenic plasmid was injected into Nematostella zygotes along with the yeast meganuclease I-SceI (New England Biolabs, Ipswich, MA) to facilitate genomic integration according to an established protocol (60) ...
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bioRxiv - Microbiology 2021Quote: ... Forward and reverse complementary primers consisting of the nucleotide codon sequence encoding for the mutation of interest were used to separately amplify the pMQ30 (for chromosomal mutations) or pMQ72 (ectopic expression) parental plasmids with the gene of interest using high fidelity Phusion polymerase (NEB). After four cycles of amplification ...
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bioRxiv - Microbiology 2020Quote: The ACE2-pLEX overexpression plasmid was generated by cloning a synthesized gBlock of the ACE2 ORF (Ref. seq. NM_001371415.1) into the pLEX plasmid via HiFi DNA Assembly (NEB Cat# M5520AA) and subsequent bacterial transformation ...
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bioRxiv - Cell Biology 2021Quote: ... were expressed with an N-terminal His8 tag followed by a 3C protease site from a pBAD-TOPO derived plasmid in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
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bioRxiv - Microbiology 2021Quote: ... Primers listed in supplemental table 1 were annealed and ligated into the digested plasmid using T4 ligase (New England Biolabs). For the generation of pGRA1.GFP.GRA2.DHFR ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 variant plasmids were constructed via ATW PCR and a KLD reaction and/or via DNA assembly using New England Biolabs (NEB) HiFi DNA Assembly (NEB E5520S) ...
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bioRxiv - Bioengineering 2021Quote: ... were either synthesized or PCR-amplified and then cloned into the px552-U6-CAG-mCherry plasmid using an NEBuilder HiFi DNA assembly kit (NEB). The AAV vectors were then sent to VectorBuilder Inc (Chicago ...
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bioRxiv - Bioengineering 2021Quote: ... were PCR-amplified using primers listed in Supplementary Table 7 and cloned into the px601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB). Similarly ...
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bioRxiv - Bioengineering 2021Quote: ... The Blunt End Ligation Method was used to construct the plasmid (Georgescu et al., 2003) with T4 DNA Ligase and T4 Polynucleotide Kinase (New England Biolabs). The sequence of the RBS of selected colonies was determined by submission of colony PCR products to Eurofins Genomics for DNA sequencing (Supplementary Table V) ...
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bioRxiv - Bioengineering 2020Quote: ... A HiFi assembly reaction was then performed to join the PCR product with the linear pEERM1 to form the assembled circular plasmid by incubating at 50 °C for one hour using NEBuilder DNA HiFi Assembly Master Mix (New England Biolabs). The HiFi reaction solution (5 μL ...
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bioRxiv - Biochemistry 2020Quote: ... The entire mDHFR ORF was PCR amplified from a mDHFR plasmid with a C-terminal AviTag fused to the mDHFR fragment using Gibson Assembly (NEB). The backbone contained a T7 promoter ...
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bioRxiv - Neuroscience 2021Quote: ... The 3’UTRs were then cloned into the cut plasmid via Gibson Assembly using the Gibson Assembly Master Mix (New England Biolabs). The 3’UTRs of ppk and chic were amplified by RT-PCR ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids to express Flag-tagged and HA-tagged proteins were created by changing the Myc-coding sequence in pCMV-Myc expression plasmids to intended tag-coding sequence using inverse PCR and NE Builder HiFi Assembly (New England BioLabs), respectively.