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Citations for New England Biolabs :
1151 - 1200 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: All plasmids were generated by using some combination of PCR amplification using Q5 DNA polymerase (NEB), HiFi Assembly (NEB ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were assembled via Gibson assembly using the HiFi DNA Assembly mix (New England Biolabs), unless stated otherwise ...
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bioRxiv - Cell Biology 2023Quote: ... The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs) and treated with a Quick Blunting Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were used for cloning plasmids smaller than 10 kb and NEBStable (NEB cat no. C3040I) competent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Assembled plasmids were transformed in the commercial Escherichia coli DH5α competent cells (New England Biolabs (NEB), USA) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid containing the origin DNA sequence was digested with HindIII and EcoRI (New England Biolabs) according to manufacturer’s protocol (Supplemental Figure S2A ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid constructs were created by Gibson cloning with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621L) or Gibson Assembly Master Mix (NEB E2611L) ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... small insertions or mutations were inserted into plasmids using Q5 site-directed mutagenesis (NEB CN# E0554S) according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Template plasmid was then removed by addition of 1 μL DpnI (New England BioLabs, Cat.#R0176), 5 μL 10X FastDigest Buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... FlucWT-HA-GFP11 and FlucDM-HA-GFP11 plasmids were constructed using site-directed mutagenesis kit (NEB) based on FlucSM-HA-GFP11 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the pAY24 plasmid digested with PstI and SmaI (New England BioLabs, #R0140 and #R0141, respectively). The sequence of all constructs was confirmed by Sanger sequencing at the Genomic Core Facility.
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Bioengineering 2023Quote: ... All plasmid construction was done by Gibson Assembly (HiFi DNA Assembly Master Mix, New England Biolabs) and validated by colony PCR and sequencing ...
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bioRxiv - Genomics 2024Quote: ... 4 μg of the barcoded plasmid library was linearized by digestion with NruI-HF (NEB #R3192) at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... Chimeras were cloned in plasmid pSHU1234 using restriction digestion with NdeI and PstI (New England Biolabs). Plasmids carrying chimeric constructs were used as templates to generate the double-stranded donor DNA used to replace the motA and motB gene on the RP437 chromosome with pomA and potD variants ...
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bioRxiv - Genomics 2024Quote: ... Plasmid libraries were amplified using 1X NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544), 1X EvaGreen dye (Biotium 31000) ...
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bioRxiv - Immunology 2023Quote: Plasmids with CAR constructs were linearized by digestion with the restriction enzyme SalI (New England Biolabs). mRNA was produced using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit ( AMB13455 ...
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bioRxiv - Genomics 2023Quote: ... Twist BioScience) and then assembled into pNL3.3 enhancer reporter plasmids using Gibson Assembly (New England Biolabs). Each cloned reporter plasmid was verified by Sanger sequencing (Quintara Bio) ...
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bioRxiv - Genomics 2023Quote: ... then ligated into a PB-CRISPRia expression plasmid linearized with BstXI and BlpI (New England Biolabs). Chemically competent Stable E ...
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bioRxiv - Synthetic Biology 2024Quote: The plasmid library containing static barcodes was generated by transforming high-efficiency competent cells (NEB C3019), then plating them onto a large surface area of LB-agar (∼30 10-cm petri dishes ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified plasmids were first amplified and linearized using Q5 Hot Start High-Fidelity DNA Polymerase (NEB); forward and reverse primers are listed in Table S5 ...
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bioRxiv - Genomics 2023Quote: ... then cloned into the EagI and EcoRI sites of the plasmid pJK03 with T4 ligase (NEB). The libraries were transformed into 5-alpha electrocompetent cells (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... We assembled the plasmids through Gibson Assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB) and followed the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid obtained by PCR was ligated using the KLD enzyme mix (NEB cat # M0554S) according to the manufacturer’s protocol before transformation ...
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bioRxiv - Biochemistry 2024Quote: ... lentiviral plasmids were constructed using a pLX317 backbone in competent Escherichia coli (New England Biolabs, C3050H). Lenti-XTM 293T (Takara ...
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bioRxiv - Biophysics 2024Quote: ... Plasmids created in this study were assembled using the Gibson Assembly Cloning Kit (New England Biolabs). All plasmid sequences were confirmed by whole plasmid sequences services provided my Plasmidsaurus SNPsaurus LLC.
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bioRxiv - Cell Biology 2024Quote: ... PCR products were inserted into the respective plasmids using the NEBuilder HiFi DNA Assembly system (NEB). All constructs used in this study were confirmed by sequencing ...
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bioRxiv - Biophysics 2023Quote: ... The 12mer DNA sequence was excised from the plasmid using Eco-RV digestion (New England BioLabs), and the excess plasmid was removed via PEG purification ...
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bioRxiv - Cell Biology 2024Quote: ... All donor plasmids were assembled by Gibson assembly using NEB Gibson Assembly Master Mix (NEB, E2611L).
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bioRxiv - Cell Biology 2023Quote: ... and G118V were generated from EGFP-PFN1 plasmid with site-directed mutagenesis (Q5 New England Biolabs) using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Microbiology 2020Quote: ... The cut plasmid and PCR product were then purified and ligated by T4 ligation (New England Biolabs). For constructing the pZKF2 (pBBR-ftsZGFP) ...
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bioRxiv - Cell Biology 2020Quote: ... and the point mutation plasmids generated using Q5 site-directed mutagenesis (New England Biolabs, Beverly, MA, USA). For lentiviral transfection ...
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bioRxiv - Cell Biology 2020Quote: All the plasmids generated for the opto-release system were cloned using the Gibson Assembly System (NEB) by combining pRK5.1 backbone with desired PCR fragments according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The size-selected fragment was cloned into the digested plasmid with NEBuilder HiFi DNA Assembly (NEB E2621), and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K) ...
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bioRxiv - Molecular Biology 2022Quote: ... the constructed plasmids were transformed by heat shock into chemically competent Top10 Escherichia coli (New England BioLabs). Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.0001ng of pUC19 plasmid methylated at 100% of CpG sites (New England BioLabs, Ipswich, MA, USA) were used as spike-in controls to determine the efficiency of APOBEC deamination and TET2 oxidation ...
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bioRxiv - Molecular Biology 2020Quote: ... pGEMHE plasmids were linearized and 5’-capped mRNA was synthesised with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and cloned into the pHERD30T-IC plasmid to replace araC-ParaBAD using Gibson Assembly (New England Biolabs). Without induction ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the plasmid pSC201 (cut with XbalI and SphI) in a Gibson-assembly mix (NEB). The reaction was transformed into E ...
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bioRxiv - Cell Biology 2019Quote: ... and 1B11 DNA and 10ng of pGRP170-DDD plasmid was digested overnight with Mfe1 (New England Biolabs). The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The gltA complement plasmid was constructed using a Gibson Assembly Cloning Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Mini-CRISPR elements and plasmid pAY5211 were digested using the restriction enzymes KpnI and BamHI (NEB, USA) and ligated using the Quick Ligation Kit (NEB ...
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bioRxiv - Immunology 2020Quote: ... Plasmid digestions were performed following manufacturer recommendations and generally subjected to Antarctic phosphatase (New England Biolabs # M0289S) treatment to prevent potential re-annealing ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed on a plasmid template using the Q5 High-fidelity 2× Master Mix (NEB, M0492L), with primers at final concentration of 500nM ...