Labshake search
Citations for New England Biolabs :
1101 - 1150 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Plasmid pl21 (TriAltus) was truncated by digestion with restriction enzymes XbaI and XhoI (New England Biolabs). To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... or GFP plasmid respectively were digested with XhoI for 1hr (New England Biolabs, Ipswich, MA, USA) for linearization ...
-
bioRxiv - Bioengineering 2019Quote: ... ligation products enclosing fragments of non-integrated transposon donor plasmid DNA were digested with DpnI (NEB) in 50 µl overnight ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and inserts and plasmid were ligated at a 1:1 molar ratio with Quick Ligase (NEB) for 20 min at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... a mini-CTX-lux (TcR) plasmid (38) was digested with EcoRI and BamHI (New England BioLabs). The promoter region of mexAB-oprM operon was amplified with EcoRI_PmexAB_Fw and BamHI_PmexAB_Rv primers ...
-
bioRxiv - Zoology 2019Quote: ... The pCMV-BI empty plasmid was digested with DraIII-HF (Cat. Nr. R3510, New England Biolabs) and NcoI-HF (Cat ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB, MA). All plasmid sequences were confirmed by DNA sequencing (Eton Bioscience ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone ...
-
bioRxiv - Synthetic Biology 2021Quote: ... plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were constructed via isothermal DNA assembly (IDA; all enzymes and dNTPs from New England Biolabs). PCR products and plasmids were cleaned up using Qiagen kits and sequenced using Eurofins Genomics ...
-
bioRxiv - Genetics 2020Quote: The pVG344 plasmid was cloned using the NEBuilder HIFI DNA Assembly Cloning Kit (New England Biolabs) to assemble four amplified fragments ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting plasmid library was then electroporated into electrocompetent NEB10β cells (New England Biolabs, Waltham, MA), which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids with mutated Marcksl1a and Marcksl1b were generated using Q5® Site-Directed mutagenesis kit (NEB). shRNA containing vectors were constructed by ligation of shRNA sequence between the EcoRI and PmeI sites downstream of the U6 promoter of modified pEGFP-U6 vector 36 (a gift from Zuoshang Xu ...
-
bioRxiv - Developmental Biology 2021Quote: ... and integrated into a BmtI and EcoRI-HF-digested pOEMGED plasmid via Gibson assembly method (NEB), with 1:3:3 plasmid to amplicon ratio ...
-
bioRxiv - Biochemistry 2020Quote: ... Plasmids were generated by Gibson assembly of PCR fragments using the NEBuilder HiFi Master Mix (NEB). Fragments were created by PCR with the relevant primers (listed in Supplementary Table 2 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Linear plasmids were subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (New England Biolabs), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmids were purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs., Ipswich, MA, USA). Linearized plasmids ...
-
bioRxiv - Genomics 2020Quote: ... The resulting linearised plasmid and double stranded oligonucleotides were ligated using Gibson Assembly Master Mix (NEB) as per manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... Mutations into these plasmids were introduced using a Q5 site-directed mutagenesis kit (New England Biolabs).
-
bioRxiv - Molecular Biology 2020Quote: ... and then inserted into pU6-2-gRNA plasmids using T4 DNA ligase (New England Biolabs # M0202S).
-
bioRxiv - Microbiology 2021Quote: All plasmids generated in this study were created by Q5 Site-Directed Mutagenesis (New England Biolabs) of existing plasmids or HiFi Gibson Assembly (New England Biolabs ...
-
bioRxiv - Cell Biology 2020Quote: ... as per manufacturer recommendations and the assembled plasmids transformed into NEB5a cells (New England Biolabs; C3019). DNA sequencing was performed to verify the integrity of the construct.
-
bioRxiv - Cell Biology 2021Quote: ... This was ligated into the plasmid pMT-FMR1-V5 (29) using the restriction enzymes XhoI (NEB) and PmeI (NEB) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The plasmid was linearized via EcoRV restriction enzyme digestion using EcoRV-HF (New England Biolabs #R3195S) before Gibson assembly.
-
bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...
-
bioRxiv - Cancer Biology 2022Quote: All the plasmid constructs were generated using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621L) as per the vendor’s instructions with some modifications ...
-
bioRxiv - Cancer Biology 2022Quote: ... were cloned by using ClaI and EcoRI restriction enzymes into the pSNAPm plasmid (New England Biolabs), with the SNAP tag in the C-terminus of the insert ...
-
bioRxiv - Cell Biology 2022Quote: ... and the PCR product was ligated into the plasmid using T4 DNA ligase (New England Biolabs), which yield pDRF1-GW AKAR3-EV.
-
bioRxiv - Microbiology 2022Quote: ... The three fragments were cloned into the pMBsacB plasmid using 2x Hifi Master Mix (M5520AA; NEB) according to manufacturer’s instructions creating the plasmid pLT001.
-
bioRxiv - Synthetic Biology 2022Quote: ... The target gene sequences were PCR amplified from the purchased plasmids using either Phusion (NEB, M0531S) or Q5 High Fidelity (NEB ...
-
bioRxiv - Plant Biology 2022Quote: ... Plasmids were also confirmed through digestion with the restriction enzyme EcoRI (New England Biolabs; Ref.: R3101S) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: Transfection plasmids were constructed by Gibson assembly using NEB Gibson Assembly Master Mix (New England Biolabs). Supplementary Table 1 provides the sequences of all primers used in this study.
-
bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... pDONR/Zeo plasmid was linearized via Polymerase Chain Reaction (PCR) with the Q5 DNA polymerase (NEB) and a pair of primers for each Cac1 mutant that anneal to the sequences flanking the section to be modified (Supplementary Table 2) ...
-
bioRxiv - Neuroscience 2023Quote: Plasmids for the trans-Tango components were generated using the Hi-Fi DNA Assembly (NEB #E5520S), BP Gateway Cloning (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmid pAR128 was generated by digesting pPL5920 with MluI-HF and NsiI-HF (New England Biolabs), PCR amplifying the UME6 locus (chrIV ...
-
bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
-
bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
-
bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit ...
-
bioRxiv - Developmental Biology 2023Quote: ... the pUC18 plasmid was subjected to digestion with the EcoRI-HF restriction enzyme (New England Biolabs), followed by a standard ligation reaction ...
-
bioRxiv - Biochemistry 2023Quote: DNA templates for the transformation assays were prepared by PCR amplification from the pUC19 plasmid (NEB), after which the PCR fragments were Dpn1-treated ...
-
bioRxiv - Genetics 2023Quote: ... the 10XUAS-DSCP promoter was amplified from plasmid pNP with Q5 High-Fidelity DNA Polymerase (NEB) using primers UP0394 and UP0395 ...
-
bioRxiv - Cell Biology 2023Quote: ... The Xlone-GFP backbone plasmid was linearized by treating with restriction enzymes SpeI-HF (NEB R3133L) and KpnI-HF (NEB R3142L) ...
-
bioRxiv - Biochemistry 2023Quote: ... The purified PCR product and pET28a plasmid were incubated with NheI and BamHI restriction enzymes (NEB) following the manufacturer’s protocol for 3 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs, Ipswich, MA), [α-35S]-CTP ...
-
bioRxiv - Microbiology 2023Quote: ... to remove residual plasmid DNA and then purified using Monarch RNA cleanup kit (New England Biolabs). RNA was reverse transcribed using SuperScript IV (Invitrogen Life Technologies ...
-
bioRxiv - Synthetic Biology 2023Quote: All PCR reactions used for plasmid assembly were done using the 2x Phusion mix from NEB, in a 50 µL volume ...
-
bioRxiv - Bioengineering 2023Quote: ... Gene inserts were first assembled into the part plasmid pYTK001 using Esp3I (NEB, Ipswich, MA, USA) unless mentioned otherwise (Lee et al ...
-
bioRxiv - Immunology 2023Quote: ... All fragments were assembled into a pLIX plasmid linearized by NheI and SalI (New England Biolabs) using HiFi DNA Assembly Kit (New England Biolabs ...
-
bioRxiv - Developmental Biology 2023Quote: ... The amplicon was subcloned into pN2-CMV:RFP plasmid using XhoI/EcoRI restriction enzyme digestion-ligation (NEB) to generate pN2-CMV:AmRps14-RFP plasmids and sequence verified ...