Labshake search
Citations for New England Biolabs :
1051 - 1100 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... Annealed products were ligated to the cleaved px330 plasmid using T4 DNA ligase (NEB, M0202T) and specific buffer (NEB ...
-
bioRxiv - Biochemistry 2024Quote: All plasmids were constructed using either NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) for Gibson assembly28 ...
-
bioRxiv - Microbiology 2024Quote: ... Purified PCR fragments were assembled with the corresponding plasmid backbones by HiFi cloning (NEB; E2621L). Subsequently the assembly mix was transformed by electro transformation into E ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 μg of plasmids were then digested overnight with the restriction enzyme CspCI (NEB, #R0645S) in a total volume of 50 μL ...
-
bioRxiv - Cancer Biology 2024Quote: ... The digested plasmid and phosphorylated oligo cassettes were ligated with T4 DNA ligase (NEB #M0202L) at room temperature for 10 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: All plasmids were constructed with NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
-
bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
-
bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal antibodies against PAR (clone 10H, Tulip BioLabs, USA), anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad ...
-
bioRxiv - Genetics 2024Quote: ... and mid-range PFG ladder (for mouse samples) (NEB Inc.) and 1 kb ladder (GeneDirex Inc.) ...
-
bioRxiv - Biophysics 2021Quote: ... The GRB2 mutagenesis plasmid (pGJJ057) was assembled by a ligation reaction (T4 Ligase, New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... 400-800 ng of the plasmid was mixed with the BsaI-HFv2 restriction enzyme (NEB, R3733S) in a 10 μL reaction volume (1 μL BsaI-HFv2 enzyme ...
-
bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
-
bioRxiv - Synthetic Biology 2022Quote: Isolated no recombinase OR gate plasmid (250 ng) was incubated with purified Cre recombinase (M0298S; NEB) at 37 °C for 1.5 hours followed by a 10-minute heat inactivation at 70 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR fragments used for plasmid construction were amplified by Q5 DNA polymerase (M0491, New England Biolabs) according to manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: ... and cloned in frame into Ch plasmid with AsiSI and NotI restriction endonucleases (New England BioLabs). The mammalian expression constructs for RFP-PCNA ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Both the PCR product and plasmid were digested by EcoRI HF and KasI (New England BioLabs) and purified ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Three microliters of prepared plasmid were linearized for integration in a twenty microliter NotI-HF (NEB) reaction for one hour and then added to the transformation mixture without purification ...
-
DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... the plasmid to integrate the hgRNA into 293T cells was digested with EcoRI-HF (NEB#R3101), then purified on a silica column (Epoch #3010) ...
-
bioRxiv - Microbiology 2019Quote: ... this DNA insert was ligated into plasmid pSRKV004 cut with BamHI and EcoRV (New England BioLabs). The plasmid pSRKV004 is a derivative of the integrating plasmid pSET152 (Hopwood ...
-
bioRxiv - Microbiology 2019Quote: ... pGRA1plus-HPT-3xHA plasmid [43] was first digested using EcoRV-HF and NcoI (New England Biolabs) for 4 hrs at 37 °C to remove the GRA1 promoter ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202). Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... Plasmids were generated by Gibson assembly of PCR fragments using the NEBuilder HiFi Master Mix (NEB) or in vivo assembly (31) ...
-
bioRxiv - Genetics 2019Quote: ... the duplexes were ligated into the BbsI site of the cut pX260 plasmid (New England Biolabs) and transformed into DH5-Alpha E ...
-
bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...
-
bioRxiv - Developmental Biology 2021Quote: ... 6-8 µg of Cas9-mSA plasmid was linearized by Not1-HF restriction enzyme (NEB #R3189S). The 8.8kb linearized fragment was cut out from a 1.5% agarose gel and DNA was extracted using QIAquick Gel Extraction Kit (Qiagen #28115) ...
-
bioRxiv - Bioengineering 2021Quote: ... The amplicons and the linearized plasmid were assembled using an NEBuilder HiFi DNA assembly kit (NEB). The assembled plasmid was named pLenti-NG-PE2-BSD.
-
bioRxiv - Biochemistry 2020Quote: ... The 186 bp fragment was excised from the plasmid by restriction digest with EcoRV (NEB: R0195) and EcoRI (NEB ...
-
bioRxiv - Biochemistry 2020Quote: ... the amplified DNA and the P4H-TM isoform 1 plasmid were both digested with BlpI (NEB) and XbaI (NEB ...
-
bioRxiv - Neuroscience 2021Quote: ... Human α-synuclein cDNA from a pcDNA3.1 plasmid was restriction digested with EcoRI and HindIII (NEB), purified and ligated in the same sites of the pAAV2.5-THP backbone to generate the pAAV2.5-TH-α-syn plasmid ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Backbone plasmids were purified from gel after digestion using the Monarch DNA Gel Extraction Kit (NEB). Ligations were carried out in 96-well PCR plates by mixing 0.2 μL of digested plasmid (3-9 ng μL−1 ...
-
bioRxiv - Genetics 2021Quote: All plasmids were constructed using Gibson-cloning with the NEBuilder HiFi DNA Assembly master mix (NEB). For the construction of the N-terminal part of the split NG-ABEmax-encoding vector (AAV-NT-ABEmax) ...
-
bioRxiv - Microbiology 2021Quote: ... the plasmid-Safe DNase-treated DNA was amplified using phi29 DNA polymerase (New England Biolabs, USA) following the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The pST76-A plasmid was cut with fast digest BsaI (New England Biolabs, Ipswitch, MA, USA) followed by phosphatase treatment (Fast AP ...
-
bioRxiv - Biophysics 2021Quote: ... the agarose-gel purified assembled insert and plasmid backbone were digested with BamHI and XhoI (NEB) and the insert ligated with T4 ligase ...
-
bioRxiv - Biophysics 2021Quote: Purified plasmids were digested at the 3’ end of the insert sequence with EcoRI-HF (NEB) to linearize the template with a 5’ overhang for in vitro transcription ...
-
bioRxiv - Cell Biology 2022Quote: Transforming plasmids were linearized by digestion with either ScaI-HF® or AseI restriction enzymes (NEB), which cut within the ampicillin resistance gene ...
-
bioRxiv - Biophysics 2022Quote: ... 2.5 kilo base pair DNA tethers were PCR-amplified from the pUC19 plasmid (New England Biolabs) with a 5’-biotinylated primer on one side and a 5’-phosphoprimer on the other side ...
-
bioRxiv - Developmental Biology 2022Quote: ... into the linearized PacI-AvrII pUASt-attB plasmid using ligation T4 strategy (New England Biolabs, Inc.).
-
bioRxiv - Microbiology 2021Quote: ... the entire pGE450 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotide TGD4981 and TGD4982 ...
-
bioRxiv - Microbiology 2021Quote: ... the entire pGE190 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotides TGD4006 and TGD5162 ...
-
bioRxiv - Microbiology 2021Quote: Complementation plasmid pMQ70_lasA was generated using the NEBuilder HiFi DNA assembly cloning kit (New England BioLabs). P ...
-
bioRxiv - Neuroscience 2020Quote: cDNA of tau 151-254 was inserted into a pMCSG17 plasmid using HiFi assembly (NEB #E2621S). The resulting vector encoded a protein consisting of a hexahistidine tag followed tobacco etch virus (TEV ...
-
bioRxiv - Plant Biology 2021Quote: ... Plasmids were isolated from positive colonies using Monarch Miniprep kit (New England Biolabs, Ipswich, MA, US) and then FastDigest XbaI or XhoI separately (Thermo Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... The 15.6-Kb ANEp8_Cas9_LIC1 plasmid was initially digested with the NotI restriction enzyme (New England Biolabs) to liberate a 5.3-Kb fragment containing the AMA1 cassette (purified by gel extraction using an Invitrogen PureLink Quick Gel Extraction Kit ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmids constructed in this study were obtained by using NEBuilder HiFi DNA assembly (New England Biolabs) method ...
-
bioRxiv - Genomics 2022Quote: ... and ligated with the aforementioned triple-digested plasmid pool using T4 DNA ligase (New England Biolabs) at 16°C overnight with a insert:vector molarity ratio of 20:1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... was sub-cloned via restriction digest of the plasmid with NsiI-HF (NEB, Cat. No. R3127) and SnaBI (NEB ...