Labshake search
Citations for New England Biolabs :
9901 - 9943 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
-
bioRxiv - Cell Biology 2024Quote: ... Library preparation for gene expression analysis was performed with the NEBNext® Poly(A) mRNA magnetic isolation module and NEBNext® UltraTM II Directional mRNA kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions with 1μg total RNA as input material ...
-
bioRxiv - Genomics 2024Quote: Illumina sequencing libraries for good quality samples (total DNA amount > 20 ng) were prepared with the standard NEBNext®Ultra™ II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA), while double stranded libraries for the samples extracted in the ancient DNA laboratory were prepared following the Meyer and Kircher protocol67 ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated using the NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L) with added indexes ...
-
bioRxiv - Microbiology 2024Quote: ... dsDNA libraries were prepared using NEBNext Ultra II FS DNA library kit for Illumina with 96-unique indexes (New England Biolabs, Massachusetts, USA; Cat. No: E6609L). DNA libraries was quantified using dsDNA High Sensitivity quantification assay on a Quibit fluorometer (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: According to the 16S Amplification SMR Tbell® Library Preparation and NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, USA) standard process database building operations ...
-
bioRxiv - Molecular Biology 2024Quote: ... All remaining steps for library construction were used according to the NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...
-
bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted from total RNA and strand-specific libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England BioLabs, Ipswich, MA, USA) following manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2024Quote: ... DNA libraries were prepared using NEBNext Ultra II FS DNA library kit for Illumina with 96-unique indexes (New England Biolabs, Massachusetts, USA; Cat. No: E6609L). DNA libraries was quantified using dsDNA High Sensitivity quantification assay on a Qubit fluorometer (Invitrogen ...
-
bioRxiv - Bioengineering 2024Quote: ... was then used as a template for in vitro transcription using HiScribe® T7 Quick High Yield RNA Synthesis Kit (NEB E2050; Ipswich, MA, USA) per the supplier’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... All remaining steps for library construction were used according to the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... BlpI-KPC-2_Rv (5’ GCT CAG CTT ACT GCC CGT TGA CGC CCA AT 3’) and the NEBuilder® HiFi DNA Assembly Cloning Kit (New England BioLabs®Inc, United Kingdom), following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Sequencing libraries were prepared with 30 ng of normalized DNA using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, Massachusetts, USA). Custom 8 bp barcodes were added for pooling ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced it as a 400- 600bp insert Illumina® library using the NEBNext Ultra II® Library Preparation kit (New England Biolabs® [NEB], Ipswich, MA USA) on an Illumina® NovaSeq lane (Novogene® ...
-
bioRxiv - Genomics 2022Quote: ... Capture and removal of CpG methylated host DNA from samples were carried out using the NEBNext® Microbiome DNA Enrichment Kit (NEB, New England Biolabs, Ipswich, MA), following the manufacturer’s recommendations with modifications (New England Biolabs ...
-
bioRxiv - Molecular Biology 2020Quote: 1000 ng per sample was then processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat # E7760L; New England Biolabs, Ipswich, USA; protocol v. v3.1_5/20) including PolyA selection ...
-
bioRxiv - Cell Biology 2021Quote: ... 80ng of genomic DNA were fragmented to around 170bp followed by End Pair and A tailing with the End Pair mix (NEB Next Ultra DNA library preparation kit). After ligation of truncated TruSeq adapters with the NEB Next Ultra Ligation Module ...
-
bioRxiv - Plant Biology 2022Quote: ... Paired end libraries for Illumina sequencing were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Inc, MA, USA) as per the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... All libraries were constructed according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). The libraries were quantified by Qubit 2.0 and their quality was assessed by TapeStation D1000 ScreenTape (Agilent Technologies Inc. ...
-
bioRxiv - Molecular Biology 2022Quote: ... then quantified with the NEBNext® Library Quant Kit for Illumina® using qPCR on a StepOne Plus real-time PCR platform (New England Biolabs® Inc., MA, USA). Following qPCR ...
-
bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
-
bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... One microgram of mRNA was used as a template for first-strand cDNA synthesis using NEBNext® Poly(A) kit (NEB #E7490, New England Biolabs, Inc.) and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760 ...
-
bioRxiv - Microbiology 2021Quote: Metagenome sequencing libraries were prepared with the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs GmbH, Frankfurt am Main, Germany) and sequenced on an Illumina NextSeq500 sequencer using 2 × 150 bp ...
-
bioRxiv - Microbiology 2020Quote: Libraries were prepared for RNA sequencing according to the instructions of the NEBNextl® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs, Ipswich, MA, USA), with the following exception ...
-
bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA; input 60 ng). Manufacturer’s instructions were followed except for Step 4 ...
-
bioRxiv - Plant Biology 2022Quote: ... The DNA library preparation was performed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (# E7645S/L, New England BioLabs, Frankfurt am Main, Germany) according to the manufacturer’s protocol.
-
bioRxiv - Plant Biology 2022Quote: DNA fragments recovered from S1 nuclease mapping and ChIP experiments were used to construct Illumina sequencing libraries using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, catalog No. E7645L) NEBNext® Multiplex Oligos for Illumina® Index Primer sets 1 ...
-
bioRxiv - Neuroscience 2023Quote: ... at -80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 100 ng RNA using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (#57760S and #57765S, New England Biolabs, Frankfurt am Main, Germany) with the NEBNext® Poly(A ...
-
bioRxiv - Cell Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA).
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
-
bioRxiv - Genomics 2024Quote: ... The small RNA library was prepared using NEB Next Multiplex Small RNA Library Prep kit for Illumina (New England Biolabs [NEB]; Ipswitch, MA, USA; ref. E7300 L) with Universal miRNA Cloning Linker (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... All remaining steps for library construction were used according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...
-
bioRxiv - Cell Biology 2024Quote: ... Retrieved RNA was further processed to build an Illumina compatible strand-specific RNA sequencing library using either NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat. E7760S, for 1F8 vs 1C9) or QuantSeq 3ʹ mRNA-Seq V2 Library Prep Kit FWD with UDI (Lexogen ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Library preparation for sRNA from sperm samples was done using the New England BioLabs kit NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 (NEB #E7300 and NEB #E7580). The provided guidelines of NEB were followed for small sample preparation until step 15 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced it as a 400- 600bp insert Illumina® library using the NEBNext Ultra II® Library Preparation kit (New England Biolabs® [NEB], Ipswich, MA USA) on an Illumina® NovaSeq lane (Novogene® ...
-
bioRxiv - Microbiology 2021Quote: The libraries for the Illumina sequencing were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs GmbH, Frankfurt am Main, Germany) according to the protocol for large fragment sizes >550 bp but with a minimal fragmentation time of only 30 s ...
-
bioRxiv - Genomics 2022Quote: ... Strand-specific RNA sequencing library and small RNA sequencing libraries were generated respectively using NEBNext® Ultra™ RNA Library Prep Kit and NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, Ipswich, MA, USA). After the cDNA synthesis and PCR amplification ...
-
bioRxiv - Genomics 2024Quote: NEB VarSkip amplicon libraries were prepared according to the NEBNext® ARTIC SARS- CoV-2 FS Library Prep Kit Workflow for Illumina® (NEB, Cat No. E7658, Ipswich, MA).
-
bioRxiv - Genomics 2024Quote: ... The small RNA library was prepared using NEB Next Multiplex Small RNA Library Prep kit for Illumina (New England Biolabs [NEB]; Ipswitch, MA, USA; ref. E7300 L) with Universal miRNA Cloning Linker (NEB ...
-
bioRxiv - Plant Biology 2021Quote: A sequencing library for the flower RNA sample was prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®, Inc., Ipswich, MA, USA) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs® ...