Labshake search
Citations for New England Biolabs :
9401 - 9450 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Libraries from purified CD14+ monocytes RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA), which allows for lower input concentrations of RNA (10ng) ...
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bioRxiv - Cell Biology 2020Quote: ... The coding sequence for Dendra2 was amplified from a plasmid clone and inserted into this backbone using the Gibson Assembly® Cloning Kit (New England Biolabs, Frankfurt, Germany); the primer sequences are available upon request ...
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bioRxiv - Developmental Biology 2021Quote: ... by in vitro transcription from v5-hPMLVI or v5-hPMLVI (K160, 490R) using a HiScribeTM T7 ARCA mRNA Kit with tailing (New England Biolabs, Ipswich, MA, USA) and a MEGAclearTM Transcription Clean-Up Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from uninfected and FV3-infected Xela DS2 cells at 24 h post-infection (hpi) and 72 hpi using the Monarch Total RNA Minipreps kit (New England Biolabs, Ipswich, United States) and genomic DNA was removed using on-column DNase I digestion ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples were sheared on a Covaris E-220 Focused-ultrasonicator and sheared DNA was then used to prepare Illumina sequencing libraries with the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs. France) and the NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) ...
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bioRxiv - Molecular Biology 2021Quote: Extraction of total RNA from HCT116 +TetOsTIR cells was performed by using Monarch® Total RNA Miniprep Kit (New England Biolabs, Inc, #T2010S). cDNA library construction using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human (Illumina #RS-122-2201 ...
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bioRxiv - Molecular Biology 2021Quote: ... Complementary DNA (cDNA) was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, Ipswich, MA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase (FLuc) RNA served as the spike in control and was generated using HiScribe T7 Quick RNA Synthesis kit (NEB, Ipswich, MA, USA) using linearized plasmid DNA encoding FLuc gene as template ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and single bands of the expected size were excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs, MA, USA). Purified PCR products were cloned into pCR-XL-TOPO™ vector using a TOPO™ XL PCR Cloning Kit following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina sequencing libraries were constructed from sheared DNA samples using an NEBNext Ultra II DNA library preparation kit (New England Biolabs, Ipswich, MA, USA) with dual indexes for multiplexing following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... libraries were prepared either directly in-house with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs), or at the sequencing company Novogene (Cambridge ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Genomics 2020Quote: ... Multiple adapters were used for pooling libraries (KAPA Single-Indexed Adapter Kit KK8700 and NEBNext® Multiplex Oligos for Illumina NEB #E7535S/L). The DNA samples were sequenced using the Illumina NextSeq 500 platform and 150-nucleotide single-end reads were generated ...
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bioRxiv - Microbiology 2022Quote: cDNA libraries were generated with the NEBNext Ultra II Directional RNA Library Prep with rRNA Depletion Kit (New England BioLabs catalog no. E7760) by the High Throughput Genomics Core at the University of Utah ...
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bioRxiv - Genomics 2022Quote: ... Capture and removal of CpG methylated host DNA from samples were carried out using the NEBNext® Microbiome DNA Enrichment Kit (NEB, New England Biolabs, Ipswich, MA), following the manufacturer’s recommendations with modifications (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645L) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Poly(A) mRNA was prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs) and 2 × 100bp paired-end sequencing at a depth of 80-100 million reads per sample was performed using the Illumina Novaseq 6000 platform by the University of Toronto Donelly Sequencing Centre ...
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bioRxiv - Molecular Biology 2021Quote: ... The rRNA-depleted RNAs were then subjected to RNA-seq library construction using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, #E7765). The final RNA-seq libraries were amplified with 7 – 8 PCR cycles.
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bioRxiv - Molecular Biology 2021Quote: ... coli using the Qiagen Miniprep kit (Hilden, Germany) and performing double restriction enzyme digests using EcoRI and HindIII purchased from New England Biosciences (NEB, Ipswich, MA, USA), using the manufacturer’s recommended procedures.
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bioRxiv - Genomics 2022Quote: ... The purified A-tailed DNA was ligated with adaptors from the Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore Technologies) and the NEBNext® Quick Ligation Module (NEB, Cat. E6056). The purified ligation products were used as the constructed sequencing library ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1000 ng of RNA was utilized for library preparation with the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 3.0μg RNA per sample was used to prepare the sequencing libraries using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). All above libraries were constructed and sequenced by Novogene Corporation (Beijing ...
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bioRxiv - Genomics 2020Quote: ... 75 ng for 37PF and 100 ng for 362YU3) were fragmented using the NEBNext® Ultra™ II FS DNA kit (New England Biolabs, USA) at 37°C for 20 minutes (resulting in DNA fragments with an average size of 350 bp) ...
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bioRxiv - Genomics 2020Quote: ... DNA was then purified and indexed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® per the manufacturer’s instructions (New England Biolabs, Inc). Libraries were sequenced on a HiSeq4000 with paired end 150bp chemistry at the Michigan State Genomics Core Facility (East Lansing ...
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bioRxiv - Immunology 2021Quote: Adherent Xela DS2 cells and any detached cells in suspension were harvested for total RNA isolation using the Monarch Total RNA Minipreps kit (New England Biolabs, Ipswich, United States) according to the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2021Quote: ... mRNA was generated from the T7 promoter in the linearized plasmids with the HiScribe T7 ARCA mRNA kit with tailing (NEB #E2060S, Ipswich, MA). We would typically use half of a kit (10 reactions ...
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bioRxiv - Molecular Biology 2020Quote: ... The Npac mutant plasmid was generated according to the manual of Q5® Site-Directed Mutagenesis Kit (E0554S, New England Biolabs. Ipswich, MA). The plasmid of full-length Npac cDNA inserted in pCAG-Neo vector was used as PCR template ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of total RNA from various tissues was used to construct RNA-seq library and sequence on the Illumina HiSeq X-10 platform following the protocol of NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Ipswich, MA, USA).
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bioRxiv - Genomics 2020Quote: ... RNAseq was performed with 100 bp paired-end reads using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was done using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to manufacturer instructions with 4 cycles of PCR amplification ...
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bioRxiv - Genetics 2021Quote: RNAseq libraries were prepared with the “protocol for library preparation of Intact RNA using NEBNext rRNA depletion kit (Bacteria) (NEB#E7850, NEB#78860) and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760 ...
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bioRxiv - Genomics 2021Quote: ... A total of eight cDNA libraries (four per experimental condition) were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (New England Biolabs, USA). The libraries were sequenced using Illumina HiSeq 2500 (paired-end reads with 150 bp length) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately one microgram of total genomic DNA from each sample was used for ~400-bp library construction with the NEBNext DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and Covaris-based fragmentation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to evaporate the samples to enable miniaturized mNGS library preparation with the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Beverly, MA, USA). Library preparation was performed per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... was prepared with the Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore) and the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (BioLabs, E7180S) using the Nanopore Oxford standard protocol for ligation sequencing.
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bioRxiv - Immunology 2021Quote: ... Libraries from purified CD14+ monocytes RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA), which allows for lower input concentrations of RNA (10ng) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was used to prepare 48 separate cDNA libraries to produce 100 base pair paired end reads using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA). Each library was individually barcoded with NEBNext dual adaptors (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were concentrated and purified with a Monarch® PCR & DNA Cleanup Kit and the entire 8 μl of eluted product was used as a substrate for Illumina sequencing library construction using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) and the protocol provided with the kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were individually barcoded and pooled into a single library with the NEB Next Ultra DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and sequenced on a HiSeq machine (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... The H543R point mutant from HF-GPR133 was generated by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat# E0554S), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from a high-titer ϕHNL01 lysate and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs, Ipswich, MA, USA). Libraries were quantified using a BioAnalyzer (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing libraries were generated from 500 ng of total RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat No. E7530), including polyA selection ...
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bioRxiv - Plant Biology 2021Quote: ... all RNA-seq libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (catalog number E7765; New England Biolabs) using 500 ng of total RNA as starting material ...
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bioRxiv - Plant Biology 2021Quote: ... promoters were inserted into the pH7WG Gateway vector (Department of Plant Systems Biology, Ghent University, Belgium (Karimi et al., 2002)) by the Gibson Assembly kit (New England Biolabs, Ipswich, MA, USA). The tagged PIN7 and PIN4 cDNA entry clones were then recombined with the modified pH7WG destination vector by the Gateway LR reaction (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... and used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), following manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... Amplicons and pCineo plasmid (linearized by NheI/EcoRI digestion) were assembled using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, cat. no. E5520), following the manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... In total 27 libraries (3 species x 9 time points) were prepared according to NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, U.S.A.) followed by paired end sequencing (100bp x 2 ...