Labshake search
Citations for New England Biolabs :
9551 - 9600 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... were used to remove rRNA from total RNA and libraries prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, New England Biolabs). The libraries were sequenced on an Illumina NovaSeq S4 (Illumina ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... NEB Ultra II directional RNA-sequencing Library Prep kit protocol was used to prepare libraries for total RNA-Seq (NEB, catalog no. E7760L). An initial concentration of 500ng of total RNA was taken for the assay ...
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bioRxiv - Genomics 2023Quote: ... and single colonies were picked 1 day later to grow up and extract plasmid DNA using a Monarch Plasmid Miniprep Kit (New England Biolabs, Cat. No. T1010L). Extracted plasmid DNA was sequence confirmed via long-read Nanopore sequencing (Primordium Labs ...
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bioRxiv - Neuroscience 2023Quote: RNA Library preparations for transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: RNA library preparations for transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... ligated to sequencing adapters and amplified using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® and NEBNext Multiplex Oligos for Illumina® (New England Biolabs). The amplified DNA (around 275 bp in size ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation was performed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs E7760S) and paired end sequencing was performed on the Nextseq 550 platform (Illumina) ...
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bioRxiv - Plant Biology 2022Quote: ... One microgram of total RNA was used to prepare each sequencing library with the NEBNext Ultra II Directional RNA library prep kit (New England Biolabs Japan, Tokyo, Japan) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Library preparation for RNA sequencing was performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA, Catalog #: E7530L), strictly adhering to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Amplified genes were purified by agarose gel electrophoresis and extraction with the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA, USA). Both the synthesized genes and PCR amplified genes were then inserted into a doubly digested BamHI/XhoI pET28b(+ ...
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bioRxiv - Biochemistry 2024Quote: N-term-His-ELMO1C438A construct was generated from N-term-His-ELMO1 construct obtained above using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S), with the following primers.
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bioRxiv - Biochemistry 2024Quote: ... B2R phosphorylation site mutants mentioned in the manuscript were generated by site-directed mutagenesis using Q5 Site-directed mutagenesis kit (NEB, Cat. No. E0554). All DNA constructs used in this study were verified by sequencing from Macrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... after which the cells were washed twice with DPBS and total RNA was extracted using the Monarch® Total RNA Miniprep Kit (NEB, Ipswich, MA). Total RNA was stored at −80°C until sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated with the NEBnext® UltraTM DNA Library Prep kit for Illumina® (New England Biolabs, Inc., Ipswich, MA, USA). After quality assessment via Qubit (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... 3 ug RNA was treated with Promega RQ1 DNase (M6101) then cleaned up with the Monarch® RNA Cleanup Kit (NEB Biolabs, T2050S) and eluted in 30 µl dH2O.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were generated at the Novogene Bioinformatics Institute using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq and MNase-seq libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat. no. E7645S) and Agencourt Ampure XP beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... using a NEBNext® single cell/low input cDNA synthesis and amplification kit (E6421L) which uses a template switching method to generate full length cDNAs (New England BioLabs, Ipswich, MA, USA). IsoSeq libraries were prepared from the cDNA according to standard protocols using the SMRTbell v3.0 library prep kit (Menlo Park ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were generated from 500 ng of total RNA using NEBNext Ultra II Directional Poly-A RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA; #E7760). cDNA library quality and quantity were assessed by TapeStation 2200 and Qubit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... multiplexed stranded cDNA sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) using poly(A ...
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bioRxiv - Physiology 2024Quote: ... Double stranded RNA was synthesized by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Whitby, ON, Canada) following manufacturers recommendations ...
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bioRxiv - Physiology 2024Quote: ... Samples were then thawed at room temperature and total RNA was isolated using the Monarch Total RNA Miniprep Kit following manufacturers protocol with an on-column DNase treatment to remove genomic DNA (New England Biolabs, Whitby, ON, Canada). Purified total RNA samples were subsequently aliquoted onto a Take3 micro-volume plate and quantified on a Synergy Multi-Mode Microplate Reader (BioTek ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit according to the manufacturer’s recommendation for Illumina® (NEB, USA). The library quality was assessed using the Agilent 2100 Bioanalyzer system ...
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bioRxiv - Immunology 2024Quote: ... were prepared and sequenced at Azenta (South Plainfield, NJ) using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The sequencing libraries were validated on the Agilent TapeStation ...
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bioRxiv - Genomics 2024Quote: ... Metagenomic libraries were generated with the NEBNext Ultra DNA Library Preparation kit following the manufacturer’s instructions (New England Biolabs, Ipswich, MA, United States) and paired-end sequenced (2 x 150 bp ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was fragmented and TruSeq-Adapters ligated with the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® (NEB) and 3’-end-fragments were finally amplified using primers with Illumina P5 and P7 overhangs ...
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Genetics 2023Quote: ... RNA libraries for RNA-seq were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760L) following manufacturer’s protocol for use with NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Some libraries have been instead prepared with the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Samples were either double indexed with a set of 60 dual-index primers ...
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bioRxiv - Physiology 2023Quote: mRNA was extracted from mesenteric and renal artery whole cell lysates using the Monarch Total RNA Miniprep Kit’ (New England Biolabs, Ipswich, Massachusetts, USA). The concentration and purity of the RNA samples were calculated using a NanoDrop 3300 Fluorospectrometer with absorbance set to 260nm and 280nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was cloned using either classical restriction and ligation ([55]; restriction enzymes, T4 DNA ligase and Quick Ligation kit from New England Biolabs, Ipswich, Massachusetts, USA) or isothermal assembly ([56] ...
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bioRxiv - Plant Biology 2024Quote: 5 μl of ribosomal depleted RNA from each sample were used to prepare 64 individually barcoded RNA-seq libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Library preparation and sequencing were performed by Novogene UK using the NEB Next Ultra RNA Library Prep Kit (New England BioLabs, Cat. No. E7530L). Samples were sequenced using 150-bp paired-end reads using NovaSeq 6000 (Illumina).
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Molecular Biology 2023Quote: ... At day 4 post nucleofection genomic DNA was extracted and 500 ng of gDNA from each sample were randomly digested with NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E6177) to obtain fragments of ca ...
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bioRxiv - Microbiology 2023Quote: ... PCR was conducted using a Rapid Barcoding Kit 96 (ONT, Oxford, UK) with Q5® Hot Start High-Fidelity 2X Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol designed for SARS-CoV-2.
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bioRxiv - Biochemistry 2023Quote: ... was constructed by replacing the gRNA region in pPvU6b-DmtRNA-BbsI (16) using the NEBuilder HiFi Assembly kit (New England BioLabs, Ipswich, MA, USA) as previously described (16 ...
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bioRxiv - Bioengineering 2023Quote: ... and whole mRNAseq libraries were generated using the NEB Next® UltraTM Nondirectional RNA Library Prep Kit for Illumina® (New England Biolabs, MA), following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760). The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA pellet was resuspended in 0.1 X TE and used for DNA library generation with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit) ...
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bioRxiv - Cell Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA was used for library preparation with NEB Next® Ultra IITM DNA Library Prep Kit for Illumina® (New England Biolabs, USA) and sequenced on Illumina NovaSeq 6000 platform (150 bp ...
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bioRxiv - Genomics 2023Quote: ... a standard whole-genome 150 base pair (bp) paired-end Illumina library was prepared using the NEBNext Ultra II library preparation kit (New England Biolabs Inc., Ipswich, USA). Finally ...
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bioRxiv - Genomics 2023Quote: ... We then ligated barcodes to the end-prepped DNA using the Native Barcoding Expansion 1–12 kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase Master Mix (New England Biolabs, Ipswich, MA, USA). We cleaned the barcoded samples using 0.9X AMPure XP beads ...
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bioRxiv - Genomics 2023Quote: ... using the Adapter Mix II from the Native Barcoding Expansion 1–12 kit and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA) as well as Quick T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... the transcriptome sequencing library was prepared and sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). All samples were clustered using the TruSeq PE Cluster Kit v3 cBot HS (Illuminia ...
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bioRxiv - Neuroscience 2023Quote: ... RNA Library preparations for Synpo-related transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...