Labshake search
Citations for New England Biolabs :
9701 - 9750 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... we prepared a 150 bp paired-end library using the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). We checked qualities and quantities of libraries using a High Sensitivity D1000 ScreenTape on an Agilent TapeStation (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A 150-bp paired-end library was prepared for each sample using the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and sequenced at high-coverage (>50-fold ...
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bioRxiv - Genomics 2024Quote: ... 100ng of RNA per each condition was used as a starting material for cDNA synthesis and library preparation with the Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, Cat.N: E6420). The quality of the RNA-seq libraries was assessed using the Agilent 2100 Bioanalyser system (Agilent).
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were synthesized from 300 ng RNA using the NEBNext Ultra II Directional RNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina (NEB; E7760 and E7735), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Next the fragments were converted to an Illumina sequencing compatible library using NEBNext® Ultra™ II DNA Library Prep kit (NEB: E7103S) and NEB E7350 adapters ...
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bioRxiv - Genomics 2024Quote: ... We employed the Luna Universal One-Step RT-qPCR Kit for the qPCR reactions (New England Biolabs, Ipswich, MA, USA, Catalog No. E3005). Each 20 μL reaction consisted of 10 μL reaction mix ...
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bioRxiv - Neuroscience 2024Quote: All site-directed mutagenesis (SDM) reactions were done with the Q5 Site-Directed Mutagenesis Kit (catalog number E0554S, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol and sequence verified by Sanger sequencing ...
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bioRxiv - Genomics 2024Quote: Strand-specific RNA-Seq libraries were constructed from 16 total RNA samples using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) with NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2024Quote: ... to evaporate to conduct miniaturized mNGS library preparation with the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Beverly, MA, USA). Library preparation was performed following manufacturer’s instructions with a few modifications ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted from VLPs using a Qiagen QIAamp Viral RNA Mini Kit and then treated with DNase I (NEB cat. no. M0303S) for 10 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... m6A enriched RNA fragments and input RNA was processed following NEBNext® Ultra™ II RNA Library Prep Kit for Illumina protocol (NEB #E7775) and subjected to sequencing using Illumina.
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bioRxiv - Cell Biology 2024Quote: ... was replaced with a ten amino acid sequence from CNGB1 (PNPEEAETES) using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Biochemistry 2024Quote: All CrPV-IRES-variant plasmid clones were obtained from Twist Biosciences and subsequently converted into the 309 residues long CrPV-IRES-mRNA by the HiScribe® T7 high yield RNA synthesis kit (New England Biolabs, Catalog # E2040S) for use in the preparation of 80S.Stop-IRES and Stop-POST5 complexes ...
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bioRxiv - Cell Biology 2024Quote: ... RNAseq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit using the NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7765 and E7600). Paired-end 2 x 50nt sequencing of libraries was performed in-house on the Illumina NextSeq2000 using the NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB #E7770, Ipswich, MA, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Library construction was carried out using the NEBNext Ultra II RNA Library Prep Kit for Illumina (Biolabs New England, NEB E7770, E7490, E7600). Libraries were randomly splitted in three groups and sequenced on three lanes with an Illumina® HiSeq4000™ machine on the GenomEast platform hosted at the IGBMC (GenomEast Platform – Institut de Génétique et de la Biologie Moléculaire et Cellulaire IGBMC ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was delivered to the Georgia Tech Molecular Evolution Core where libraries were prepared with the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E6177) using the Protocol for FS DNA Library Prep Kit (E7805 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and strand-specific libraries were prepared (Azenta, Inc., South Plainfield, NJ) using NEBNext Ultra II RNA Library prep kits (New England Biolabs, Ipswich, MA, USA). Sequencing libraries were validated (Agilent TapeStation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Library construction was carried out using the NEBNext Ultra II RNA Library Prep Kit for Illumina (Biolabs New England, NEB E7770, E7490, E7600). Libraries were randomly splitted in three groups and sequenced on three lanes with an Illumina® HiSeq4000™ machine on the GenomEast platform hosted at the IGBMC (GenomEast Platform – Institut de Génétique et de la Biologie Moléculaire et Cellulaire IGBMC ...
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bioRxiv - Developmental Biology 2024Quote: ... The purified PCR products were served as DNA template for in vitro transcription using HiScribe® T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA) with tailing following manufacture’s instruction ...
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bioRxiv - Genetics 2024Quote: ... followed by a 10-fold dilution of the universal adaptor and 6 cycles of PCR with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, Cat. No. E7805S), and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genetics 2024Quote: ... 300ng of RNA was utilized for cDNA synthesis using the NEBNext Single Cell/Low Input cDNA Synthesis kit (New England Biolabs, Ipswich, MA, USA). For this purpose ...
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bioRxiv - Genetics 2024Quote: ... PCR products of interest were purified for Sanger sequencing (Genewiz Inc.) either via gel extraction with Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.) or directly after PCR using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs® Inc.).
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bioRxiv - Evolutionary Biology 2024Quote: ... Sequencing libraries were made with 5 ng DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB #E7103S/L) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The final rRNA depletion probe pool was made by combining the NEBNext rRNA Depletion Solution from NEBNext rRNA Depletion Kit V2 (Human/Mouse/Rat; New England Biolabs; Cat. No. E7405) and NEBNext rRNA Depletion Kit V2 (Bacteria ...
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bioRxiv - Evolutionary Biology 2024Quote: ... a 150bp paired-end library was prepared using the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA), and whole-genome sequenced on the Illumina NovaSeq platform (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Library preparation for sRNA from sperm samples was done using the New England BioLabs kit NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 (NEB #E7300 and NEB #E7580). The provided guidelines of NEB were followed for small sample preparation until step 15 ...
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bioRxiv - Genetics 2021Quote: ... Bands corresponding to small RNAs were collected and their content was recovered using the Monarch DNA Gel Extraction kit (NEB #T1020 New England Biolab). The sRNA collected were turned into libraries using the NEBNext Multiplex Small RNA Library Prep Set (NEB #E7300S New England Biolab) ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Microbiology 2021Quote: ... The RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration was determined using a Nanodrop and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Molecular Biology 2021Quote: ... following the manufacturer’s instructions and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Evolutionary Biology 2021Quote: Extracted and purified RNA was reverse transcribed into first-strand copy DNA (cDNA) using the ProtoScript® II Reverse Transcriptase Kit (NEW ENGLAND BioLabs®Inc, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was conducted using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific RNASeq libraries were created using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #6420L; New England Biolabs, Inc., Ipswich, MA, USA). The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626 ...
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
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bioRxiv - Genomics 2020Quote: Whole Genome DNA libraries with an average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Genomics 2020Quote: ... Approximately 300 ng of genomic DNA was used to prepare DNA sequencing libraries for each parent following the protocol of NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB Cat No. #E7805S/L). The libraries were sent to Novogene (https://en.novogene.com/ ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of RNA was used for cDNA library construction using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7760S, E7335). Library size (~350 bp ...
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bioRxiv - Plant Biology 2021Quote: The RNA-seq libraries were constructed with the NEB Next® Ultra™ II RNA Library Prep Kit for Illumina® following manufacturer’s instruction (NEB, Cat#E7775).
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bioRxiv - Plant Biology 2020Quote: ... A total of 12 sRNA libraries were constructed from 5 μg of size-selected RNA using the NEBNext® Small RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) as per the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Libraries were prepared from 1 μg of total RNA using NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) by poly(A ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were generated from a total amount of 3 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was inserted to the PstI and BglII sites on the pWALIUM20 vector (Perkins 2015) by Gibson-assembly (NEB HiFi DNA Assembly Kit) to generate pWALEXA20 (White-AttB-LexAop for shRNA expression) ...
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bioRxiv - Microbiology 2021Quote: ... D186N were first introduced into the Rluc-CV-B3 reporter viruses with Q5 site-directed mutagenesis kit (New England Biolabs, Bioké, Leiden, The Netherlands)52,53 ...
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bioRxiv - Genetics 2022Quote: ... We inserted TFT reporters into BFA0190 by digesting the plasmid with SwaI and inserting TFT components between the LYP1 flanking sequences using isothermal assembly cloning (Hifi Assembly Cloning Kit; New England Biolabs [NEB], Ipswich, MA, USA). The 5’ and 3’ LYP1 flanking sequences in each TFT plasmid contain natural SacI and BglII restriction sites ...