Labshake search
Citations for New England Biolabs :
9851 - 9900 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... RNA quality was checked on a BioAnalyzer and Strand-Specific RNA-seq library preparation was performed with rRNA depletion using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, E7760) and the NEBNext® Globin & rRNA Depletion Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... iScript™ cDNA Synthesis Kit (Bio Rad, Cat. No. 1708891) was used for cDNA preparation and Luna® Universal qPCR Master Mix (NEB, Cat. No. M3003L) was used for subsequent qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). Following adapter ligation and purification qPCR was used to determine optimal cycle numbers for library PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... This elution product was then ligated to the RTA adapter (Oxford Nanopore Technologies) provided in both the RNA002 and RNA004 sequencing kits using high concentration T4 DNA ligase (NEB, M0202M, 2,000,000 U/mL) at a molar ratio of 3 fold more ligated tRNAs than RTA adapter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... USA) and used to generate sequences with the desired amino acid substitution using Q5 Site-Directed Mutagenesis Kit (NEB, via BioTek, Praha, Czech Republic). Primers were designed using NEBaseChanger (https://nebasechanger.neb.com/) ...
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bioRxiv - Genomics 2024Quote: ... mRNA Magnetic Isolation Module E7490L and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® E7760L (New England Biolabs Inc.). Quality and quantity evaluation of the libraries were conducted using the High Sensitivity D1000 Screen Tape on an Agilent 4150 TapeStation instrument ...
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bioRxiv - Genomics 2024Quote: ... 3 μg of RNA was used to create sequencing libraries using the NEBNext Ultra TM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, United States) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: 1 µg of DNase-treated total RNA for each sample (whole body, dissected head, thorax, and abdomen) was reverse transcribed using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... In vitro-transcription of the annealed DNA oligonucleotides was carried out using HiScribe™ T7 High Yield RNA Synthesis Kit (Catalog no. E2040S, New England Biolabs Pte. Ltd., Singapore), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit with ribodepletion (New England Biolabs, NEB protocol Version 4.0_4/21), starting from 51 ng of input RNA ...
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bioRxiv - Cell Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit with ribodepletion (New England Biolabs, NEB protocol Version 4.0_4/21), starting from 51 ng of input RNA ...
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bioRxiv - Bioengineering 2024Quote: A library containing 252 mutations across the Sm binding and U7 hairpin sequences was synthesized (Twist Biosciences, Suppl. Table 2) and cloned onto three antisense gRNA plasmids in duplicate pools (NEB Golden Gate Assembly kit), each comprising approximately 3000 bacterial colonies ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA Magnetic Isolation Module and cDNA Library was prepared with NEB E7760 NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs E7490) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared with the NEBNext® Ultra II Directional RNA Library Prep for Illumina® kits (New England Biolabs, Ipswich, MA) after ribosomal RNA depletion following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic libraries were prepared using the NEBNext® Ultra TM II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, United States) using reagents at half volumes following Hale et al ...
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bioRxiv - Systems Biology 2020Quote: ... mRNA-seq libraries were prepared for sequencing using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, Ipswich, MA, USA) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... RNA libraries were constructed according to standard protocol using NEB Next® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). Ribosomal RNA depletion was carried out using Ribo-zero rRNA Removal Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... 400ng of RNA was used to generate sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total RNA in a maximal volume of 6 µ L is used for cDNA synthesis using NEB PhotoScript® First Strand cDNA Synthesis Kit (Catalog # E6300L, NEB Inc, Ipswitch, MA) as instructed in the manufacturer standard protocol ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA fragments corresponding to the full-length ORFs of the candidate genes were then amplified via PCR with Q5® High-Fidelity Polymerase PCR kit (NEB, Frankfurt am Main, Germany) using gene-specific gateway primers (Supplementary Table S9) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs, Ipswich, MA, USA) and sequenced on two lanes (two chips ...
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed at the McGill Genome Center using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following manufacturer’s recommendations and sequenced by Illumina HiSeq 2×150 cycles run (Illumina Inc. ...
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bioRxiv - Genomics 2020Quote: ... double stranded cDNA synthesis was performed from DNase pretreated total nucleic acid extracts using the NEBNext® Ultra™ II RNA First Strand Synthesis Module and NEBNext® Ultra™ II Non-Directional RNA Second Strand kit (New England Biolabs, MA, USA). The enrichment was based on a hybridization step with a respiratory viruses biotinylated adjacent oligoprobes panel (16 ...
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bioRxiv - Systems Biology 2021Quote: ... The remaining mRNA was used for stranded RNA-seq library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genetics 2020Quote: ... 5 ng of fragmented DNA were then used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), as per manufacturer instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... mRNA Magnetic Isolation Module in combination with the NEBNext® Ultra™ II Directional RNA Library Prep Kit and indexed with NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) (New England Biolabs, Ipswich, Massachusetts, USA). Fragmentation was carried out using incubation conditions recommended by the manufacturer for samples with a RIN ≥7 to produce RNA sizes ≥ 300bp (94°C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The IL-2 signal sequence that was present in the original mCherry-progranulin plasmid was later replaced with progranulin signal sequence by site-directed mutagenesis (NEB Q5 site directed mutagenesis kit). For cloning of Prosaposin-RUSH (Str-KDEL_Prosaposin-SBP-mCherry) ...
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bioRxiv - Genomics 2022Quote: ... The RNA samples and standards were amplified using the Luna® Universal Probe One-Step RT-PCR Kit (E3006, BioLabs®Inc, Ipswich, Massachusetts, USA), with 0.6 mM primer and 0.2 mM probe concentrations (Table S4) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription reactions were then used as input material for NEBNext® Ultra™ II DNA library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), using NEBNext Multiplex Oligos for Illumina® (Unique Dual index UMI Adaptors DNA Set 1 ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit, New England Biolabs, GmbH, Frankfurt am Main, Germany). PCRs were performed using the Q5 polymerase (Q5 Hot Start High-Fidelity ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Neuroscience 2022Quote: ... Polyadenylated RNA was captured from 50 ng of total RNA per sample and libraries were prepared using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Paired-end sequencing was done at the Vanderbilt VANTAGE core laboratory (Vanderbilt University ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs, Ipswich, MA, USA). Each library was individually labeled and subjected to 12 PCR cycles for final enrichment and pooled in equal quantities ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Developmental Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) were used based on the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... dual unique indexed libraries were made using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Manufacturer protocol was modified by diluting adapter 1:30 and using 3μL of this dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing of RNA samples was performed by Azenta Life Sciences (Burlington, Massachusetts, US) with NEBNext®Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, Massachusetts, US) for library preparation.
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bioRxiv - Genomics 2023Quote: ... 1 µg of the resulting DNA was used for library preparation using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB cat. E7103). Library PCRs were supplemented with 1× EvaGreen (Jena Bioscience cat ...
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bioRxiv - Biophysics 2023Quote: ... The mutation was introduced with an oligonucleotide designed to alter codon Arg90 (CGC) to Ser (AGC) using a Q5TM site-directed mutagenesis kit (#EO552, #EO554S, New England BioLabs GmbH, Frankfurt am Main, Germany). The successful introduction of mutations was confirmed by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting mRNA material was used to prepare the libraries with the use of NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: Site-specific mutagenesis was performed with the Q5® Site-Directed Mutagenesis Kit (based on PCR-amplification) according to the manufacturer’s protocol (New England Biolabs GmbH, Frankfurt am Main, Germany). Oligonucleotides were ordered from metabion GmbH (Planegg/Steinkirchen ...
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bioRxiv - Molecular Biology 2023Quote: ... For the library preparation of the pools the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich (MA) – United States of America ...
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bioRxiv - Neuroscience 2023Quote: ... Both input samples without IP and m1A input samples were used for library construction using an NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., MA, USA). Eluted RNA fragments were converted to cDNA and sequenced or treated to induce partial m1A to m6A conversion before cDNA synthesis ...
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bioRxiv - Immunology 2022Quote: ... Libraries for bulk RNA sequencing were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, cat. #E7490 and #E7760). The input RNA and the resulting libraries were analyzed with Agilent 4200 Tapestation for quality assessment ...
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bioRxiv - Immunology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Paired-end libraries of 2 x 150 bp fragments with an insert size of 330 bp were prepared with NEBNext® Ultra™ II DNA Library Prep Kits (New England Biolabs, Ipswich, MA, USA), and sequencing was performed on a HiSeq4000 Illumina sequencer (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The constructs M1 and M2 were used as templates to generate higher-order mutants by site-directed mutagenesis PCR (NEB, Q5 Site-Directed Mutagenesis Kit) using the primers listed in Table S2 ...