Labshake search
Citations for New England Biolabs :
9751 - 9800 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...
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bioRxiv - Genomics 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
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bioRxiv - Genetics 2022Quote: ... with insert-specific primers LC186 and 187) between hlh-30 and 3XFLAG-tag segments with HiFi cloning (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs Inc., Ipswich, MA). Lastly ...
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bioRxiv - Cancer Biology 2022Quote: ... CUT&RUN barcoded libraries for Illumina sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) accordingly with Zhu et al.(Zhu et al. ...
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bioRxiv - Plant Biology 2021Quote: ... and phloem tissues were also prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs®) ...
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bioRxiv - Genomics 2020Quote: Genomic DNA purified from one single haploid drone of each species was used to generate one “fragment” library with an insert size of 400 or 450 bp using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA). The prepared fragment libraries were sequenced on an Illumina HiSeq 2500 platform with a read length of 250 bp to produce overlapping paired-end shotgun reads (2 × 250 bp) ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (cat no. E7770, New England Biolabs, Ipswich MA). Single-end sequencing was performed using the Illumina NextSeq500 platform according to standard operating procedures.
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bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Illumina MiSeq adaptors were ligated onto the amplicon library using a NEBNext® DNA MasterMix for Illumina Kit (New England BioLabs, Ipswitch, MA, USA) and sequences were generated using a MiSeq instrument (2 × 250 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for ChIP-seq were constructed using NEBNext® UltraTM DNA Library Prep Kit for Illumina (Cat. #E7370; New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The extracted DNA from water and soil underwent a further purification step using Monarch®PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA). DNA Library preparation was conducted using Native barcoding genomic DNA Kit SQK-LSK 109 combined with EXP-NBD104 (Oxford Nanopore Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... 100 ng total RNA were used as input for ribosomal RNA (rRNA) depletion using the NEBNext rRNA depletion kit (New England BioLabs inc., product code: E6350) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... In vitro transcription was performed in 20 µL reaction volume using HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs, catalog number E2040S) following the Capped RNA Synthesis protocol provided by the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... DNA samples were purified before and after PCR with the Monarch® PCR and DNA Clean-Up Kit (New England Biolabs, Ipswitch, MA, USA). Individual sample libraries were pooled ...
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bioRxiv - Bioengineering 2022Quote: ... The library was prepared by a Ribo-Zero protocol (250-300 bp insert strand specific library with rRNA removal using NEB Ribo- Zero Magnetic Kit). Paired-end sequencing produced 150 bp reads ...
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bioRxiv - Evolutionary Biology 2020Quote: ... leprae positive faecal and necropsy samples (Supplementary Information Table 1) were converted into dual-indexed libraries using the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA)57,58 ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μg RNAs for each sample were used for library construction using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA, cat. no. E7530) according to the instruction and RNA-Seq on Illumina Hiseq 2500 platform.
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bioRxiv - Developmental Biology 2020Quote: ... digested pCFD6 backbone to generate UAS-t::gRNA-twi4x using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Cat. No: R5520S), following the instructions provided by the manufacturers.
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... The DAP gDNA library was prepared using the kit from NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB #E6040S/L). ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A ...
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bioRxiv - Cell Biology 2020Quote: ... and the resulting derivatives were linearized and used as templates for in vitro RNA transcription with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA); 1.5 μg template was incubated for 4 hrs following kit protocols ...
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bioRxiv - Genomics 2021Quote: Whole Genome DNA libraries with average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Plant Biology 2021Quote: ... cablin seedlings and were introduced into the expression vector pET-28a (+) between the restriction sites of NdeI and BamHI using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, Catalog number: E5520S), in each case generating a fusion gene that encoded a “tag” of HIS6 residues at the N-terminus for expression in E ...
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bioRxiv - Microbiology 2020Quote: ... samples were treated using a Pan-Prokaryote riboPOOL™ kit (siTOOLs Biotech Gmbh, Planegg, Germany) coupled with streptavidin magnetic beads (New England Biolabs, Ipswich, MA, USA). mRNA was eluted in 50 μl of nuclease-free water and then cleaned and concentrated using a Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... Three recB fragments were directly assembled into pET28c vector in frame with the N-terminal 6xHis tag employing NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Inc., Ipswich, MA, USA). Wild type RecC encoding gene was individually sub-cloned into pET28c vector in frame with the N-terminal 6xHis tag ...
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bioRxiv - Genomics 2021Quote: ... cDNA libraries were synthesized using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (Both: New England Biolabs, Ipswich, Massachusetts) and subsequently sequenced in a paired-end mode with 75bp read length on NextSeq500 (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg gDNA was then used to prepare the gDNA sample library using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and sequencing libraries were constructed using a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, E7420L) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
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bioRxiv - Plant Biology 2023Quote: ... and then the sequencing library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan Inc., Tokyo, Japan). The library was sequenced using the illumina NovaSeq 6000 platform with S4 flow cells to produce 150 bp paired-end and dual index reads ...
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bioRxiv - Cancer Biology 2022Quote: ... the fragments were purified and used for adapter ligation and library amplification using the NEBNext Ultra II Fs DNA Library kit for Illumina (NEB # E7805, New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... UAS expression constructs were generated by combining the linearized pGW-HA.attB backbone and each individual ZIKV fragment in Gibson assembly reactions (NEB Gibson Assembly Cloning Kit, E5510S). To generate NS4A::2K ...
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bioRxiv - Microbiology 2023Quote: ... and the expression levels of genes were quantified with the Luna® universal one-step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) with the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (New England BioLabs, Frankfurt am Main, Germany). Sequencing was performed by using the HiSeq2500 instrument (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... where a cDNA library was prepared with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) and 150 paired-end transcriptome sequencing was conducted using an Illumina NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... Whole genome enzymatic methyl-seq libraries were prepared using the NEBNext® Enzymatic Methyl seq kit (New England BioLabs® Inc., Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were further lysed with probe sonication and the lysate was denatured by adding 10 µL of denaturing buffer (NEB PNGase F kit, P0709) and heating at 100 °C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were generated from 1 µg total RNA per sample using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... dsRNA was synthesized from T7-linked DNA using the HI Scribe™ T7 High Yield RNA Synthesis Kit (New England, Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... strand-specific total-transcriptome RNA sequencing libraries were prepared by incorporating dUTPs in the second-strand synthesis using NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA). The isolated cell RNA-seq libraries were prepared using the Ovation® SoLo® RNA-Seq library preparation kit (Tecan ...
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bioRxiv - Developmental Biology 2022Quote: The RNA-seq libraries were prepared from 100 μg of total RNA per genotype with NEBNext Ultra II RNA Library Prep Kit mRNA Isolation Module (NEB, Cat# E7775S, E7490S, E7500S). Quality control and sequencing of the RNA-seq libraries were performed by GENEWIZ ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared from the enriched mRNAs using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA). The library concentration was determined in a Qubit3 Fluorometer using the Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Molecular Biology 2023Quote: ... These immuno-enriched DNA samples could be used for NGS after DNA library construction with NEBNext® UltraTM II DNA Library Prep Kit (NEB, USA, Catalog #: E7645L). The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co. ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA-seq libraries were constructed using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Finally ...
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bioRxiv - Evolutionary Biology 2024Quote: ... For Psav we used a standard miniaturized protocol to prepare DNA libraries for sequencing using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA)) ...
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bioRxiv - Zoology 2024Quote: ... Purified dsRNA templates were reamplified from the L4440 vector with T7 promoter oligonucleotide sequences and used for dsRNA synthesis by in vitro transcription using HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from agar plate-grown seedlings by acid guanidinium thiocyanate-phenol-chloroform-based extraction and purified from the aqueous phase using the Monarch RNA Clean Up Kit (New England Biolabs, Ipswich, MA, USA; NEB). Genomic DNA in the samples was removed using TURBO™ DNase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Strand-specific libraries were generated from the rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... and the processed RNA samples were used to construct RNA-seq libraries using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA). Then the RNA libraries were sequenced using the Illumina HiSeq 4000 platform to generate 150bp paired-end reads ...