Labshake search
Citations for New England Biolabs :
9801 - 9850 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... we used PCR with long protruding primers to add GGS sequences and BsaI sites directly to the genes of mTurquiose2 and mVenus and assembled them with the pET09 via NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2) (NEB, Ipswich, MA). Control proteins were PCR amplified from either E.coli DH5 αgenome or in-house generated constructs ...
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Benzonase was inactivated using 50 mM EDTA and DNA was then extracted using Monarch Genomic DNA purification kit (New England Biolabs, Ipswich, MA, USA, #T3010S).
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bioRxiv - Neuroscience 2023Quote: ... 50 ng total RNA were used as input for ribosomal RNA (rRNA) depletion using the NEBNext rRNA depletion kit (New England BioLabs inc., product code: E6350) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: For library construction the ChIP purified DNA was used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645L) with modifications of the manufacturer instruction as we assembled half volume reactions ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were obtained using the NEB-Next® UltraTM II RNA library prep kit for Illumina® (E7770S, New England Biolabs, Ipswich, MA). The isolated mRNA was fragmented ...
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bioRxiv - Microbiology 2023Quote: ... Point mutations to generate the mutant variants were introduced using the Q5® Site-Directed Mutagenesis Kit purchased from New England Biolabs (NEB, Ipswich, MA, USA). Primers were acquired from Sigma Aldrich (St ...
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bioRxiv - Microbiology 2023Quote: ... The resultant fragments were cloned into plasmid pKNG101 by using the NEBuilder® HiFi DNA Assembly Cloning kit (New England Biolabs, Ipswich, MA, USA) (33) ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries using 500 ng of good-quality RNA (RIN ˃7and a NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA, #E7760L) at the Genome Québec core facility and paired-end sequenced on a NovaSeq6000 S4 sequencer ...
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bioRxiv - Microbiology 2023Quote: ... the products were then used to construct sequencing libraries using the NEB Next® Ultra II™ DNA Library Prep Kit for Illumina® (New England Biolabs, USA) following the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2023Quote: ... Site directed mutagenesis was then performed for the promoter region of iLight repressor through using NEB Q5® Site-Directed Mutagenesis Kit with non-overlapping primers or NEBuilder® HiFi DNA Assembly (New England BioLabs, MA, USA) with overlapping primers ...
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bioRxiv - Bioengineering 2023Quote: ACE2 plasmids with different cytoplasmic tail lengths were generated using the Q5® Site-Directed Mutagenesis (SDM) Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with Unique Dual Index UMI Adapters RNA Set1 (NEB, Franklin Lake, NJ, USA) were used in the succeeding steps according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat. No. E7645) and the NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Cat ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis thaliana RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Preparation Kit (New England BioLabs, MA, USA). Five hundred ng of total RNA was taken for mRNA isolation ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... 200 ng of DNA was used for transcription by T7 polymerase (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB, Ipswich, MA, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... We created DNA libraries with an average library size of 320 bp using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Library preparation and sequencing were prepared with Illumina-compatible NEBNext® Ultra™ II using Directional RNA Library Prep Kit (New England BioLabs, MA, USA). Viral total RNA was taken for fragmentation and priming ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We used a standard miniaturized protocol to prepare DNA libraries using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA))56 ...
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RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: ... and pELS53 [Peft-3::Cas9 + rtel-1 sgRNA] plasmids were generated for this study by modifying the pDD162 plasmid (Dickinson, 2013) using Q5 Site Directed Mutagenesis Kit (NEB, Cat# E0554S, Ipswich, MA).
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bioRxiv - Microbiology 2022Quote: ... with duplicate sample in each group) was depleted of bacterial rRNA using NEBNext® rRNA depletion kit (Cat. no. E7850X, New England Biolabs, Ipswich, MA,USA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... and reversely transcribed into cDNA from 1 μg of mRNA template using the M-MuLV cDNA Synthesis Kit (New England Biolabs, Frankfurt am Main, Germany) according to the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated from 1 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Whole mRNAseq libraries were generated using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). The clustering of index-coded samples was then performed on a cBot Cluster Generation System ...
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bioRxiv - Cancer Biology 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, Cat. No. E7490L, E7760L) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Systems Biology 2023Quote: ... Purified DNA fragments were assembled by One Step Cloning Kit (Vazyme Co., Ltd. Cat. C113) or Golden gate assembly54(NEB, Cat. M0202 and R3733). Sequence contents of genetic parts are collected in Supplementary Table 11.
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bioRxiv - Evolutionary Biology 2023Quote: ... Individual genomic libraries for the NGS experiments were constructed with the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) with inputs ≤ 100 ng (Supplementary information) ...
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bioRxiv - Microbiology 2023Quote: ... samples were repaired and end-prepped using the NEBNext® FFPE DNA Repair Mix and NEBNext® Ultra™ II End Repair/dA-Tailing kits (New England Biolabs, USA). DNA was cleaned using Ampure XP Beads (Beckman Coulter ...
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bioRxiv - Zoology 2023Quote: ... The recombinant clones containing these constructs were then grown overnight in LB liquid media containing 25 μg/ml of Zeocin™ antibiotic and plasmid DNA isolation was isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Whitby, ON). Base-pair accuracy and orientation of the receptor constructs were confirmed by Sanger sequencing (Center for Applied ...
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bioRxiv - Bioengineering 2023Quote: ... RNA synthesis was conducted via in vitro transcription using a HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs, NEB, Ipswich, MA). After DNase I (RNase-free ...
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bioRxiv - Plant Biology 2023Quote: ... High quality of RNA was used to prepare RNA-Seq libraries with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England BioLabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Frankfurt am Main, Germany). To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... and polyA mRNA sequencing libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat# E7760L and E7765L) per the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ARTIC V4 amplicon libraries were prepared according to the NEBNext® ARTIC SARS- CoV-2 Library Prep Kit Workflow for Illumina® (Discontinued, New England BioLabs, Ipswich, MA). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) and reverse-transcribed to cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Cat no. E3010) with random hexamers and oligo dT ...
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bioRxiv - Microbiology 2024Quote: cDNA was synthesized from the total RNA samples using either the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA), following the standard protocol with Random Primer Mix ...
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bioRxiv - Genomics 2024Quote: Adult worm Illumina sequencing libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Cat: E7805L) and DNA concentrations measured with the dsDNA High Sensitivity Assay kit on a Qubit 4 Fluorometer (both Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries for bulk RNA sequencing were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, cat. #E7490 and #E7760). The input RNA and the resulting libraries were analyzed with Agilent 4200 Tapestation for quality assessment ...
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bioRxiv - Pathology 2024Quote: ... CrRNA was synthesized from dsDNA template by overnight incubation at 37°C using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Frankfurt am Main, Germany). Transcribed crRNA was cleaned up using Monarch@ RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid was directly amplified using Q5 polymerase using Polymerase chain reaction (PCR) (Q5® High-Fidelity PCR Kit, New England Biolabs, Ipswich, MA, USA). PCR was carried out following manufacturer protocol (Table S3 ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II FS DNA library kit for Illumina with 384 unique indexes (New England Biolabs, Ipswich, MA, United States). Double-stranded DNA libraries were then sequenced using the HiSeq X10 with 150 bp paired-end chemistry (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, New England Biolabs, Ipswich, Massachusetts, USA) and following the manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, Cat. No. E7770) using compatible NEBNext® Oligos for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... Mutations were introduced via site-directed mutagenesis into the human GARS1 cDNA using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA). Next ...
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bioRxiv - Neuroscience 2024Quote: High molecular weight (HMW) DNA was extracted from isolated cells using the Monarch HMW DNA extraction kit (New England Biolabs, Ipswich, MA, USA, #T3050L) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested by scraping and total RNA was isolated using the Monarch Total RNA Miniprep kit (T2010S, New England Biolabs Inc., Ipswich, MA, USA) according to the supplierś instructions ...
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bioRxiv - Zoology 2024Quote: ... and post-aestivation stages) were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module followed by NEBNext kit (E7490, NEB, Ipswich, MA, USA). The quality and quantity of the libraries were checked on an Agilent 2100 Bioanalyzer using the Agilent DNF-935 Reagent Kit (Agilent Technologies ...
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bioRxiv - Physiology 2024Quote: ... All remaining steps for library construction were done according to the NEBNext Ultra™ II Non-Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...