Labshake search
Citations for New England Biolabs :
9651 - 9700 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... cDNA fragments corresponding to the full-length ORFs of the candidate genes were then amplified via PCR with Q5® High-Fidelity Polymerase PCR kit (NEB, Frankfurt am Main, Germany) using gene-specific gateway primers (Supplementary Table S9) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs, Ipswich, MA, USA) and sequenced on two lanes (two chips ...
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed at the McGill Genome Center using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following manufacturer’s recommendations and sequenced by Illumina HiSeq 2×150 cycles run (Illumina Inc. ...
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bioRxiv - Genomics 2020Quote: ... double stranded cDNA synthesis was performed from DNase pretreated total nucleic acid extracts using the NEBNext® Ultra™ II RNA First Strand Synthesis Module and NEBNext® Ultra™ II Non-Directional RNA Second Strand kit (New England Biolabs, MA, USA). The enrichment was based on a hybridization step with a respiratory viruses biotinylated adjacent oligoprobes panel (16 ...
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bioRxiv - Molecular Biology 2019Quote: ... 400ng of RNA was used to generate sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2021Quote: ... The remaining mRNA was used for stranded RNA-seq library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... was used for poly A selected RNA-seq library preparation using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (mRNA) (New England Biolabs Cat#E7530S). Biological replicates were individually barcoded ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genetics 2020Quote: ... 5 ng of fragmented DNA were then used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), as per manufacturer instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... mRNA Magnetic Isolation Module in combination with the NEBNext® Ultra™ II Directional RNA Library Prep Kit and indexed with NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) (New England Biolabs, Ipswich, Massachusetts, USA). Fragmentation was carried out using incubation conditions recommended by the manufacturer for samples with a RIN ≥7 to produce RNA sizes ≥ 300bp (94°C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The IL-2 signal sequence that was present in the original mCherry-progranulin plasmid was later replaced with progranulin signal sequence by site-directed mutagenesis (NEB Q5 site directed mutagenesis kit). For cloning of Prosaposin-RUSH (Str-KDEL_Prosaposin-SBP-mCherry) ...
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bioRxiv - Genomics 2022Quote: ... The RNA samples and standards were amplified using the Luna® Universal Probe One-Step RT-PCR Kit (E3006, BioLabs®Inc, Ipswich, Massachusetts, USA), with 0.6 mM primer and 0.2 mM probe concentrations (Table S4) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Biophysics 2022Quote: Site-specific mutagenesis was performed with the Q5® Site-Directed Mutagenesis Kit (based on PCR-amplification) according to the manufacturer’s protocol (New England Biolabs GmbH, Frankfurt am Main, Germany). Oligonucleotides were ordered from metabion GmbH (Planegg/Steinkirchen ...
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bioRxiv - Molecular Biology 2023Quote: ... For the library preparation of the pools the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich (MA) – United States of America ...
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bioRxiv - Neuroscience 2023Quote: ... Both input samples without IP and m1A input samples were used for library construction using an NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., MA, USA). Eluted RNA fragments were converted to cDNA and sequenced or treated to induce partial m1A to m6A conversion before cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Neuroscience 2022Quote: ... Polyadenylated RNA was captured from 50 ng of total RNA per sample and libraries were prepared using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Paired-end sequencing was done at the Vanderbilt VANTAGE core laboratory (Vanderbilt University ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated mRNA material was used to prepare the libraries using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs, Ipswich, MA, USA). Each library was individually labeled and subjected to 12 PCR cycles for final enrichment and pooled in equal quantities ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Developmental Biology 2023Quote: ... The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) were used based on the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription reactions were then used as input material for NEBNext® Ultra™ II DNA library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), using NEBNext Multiplex Oligos for Illumina® (Unique Dual index UMI Adaptors DNA Set 1 ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit, New England Biolabs, GmbH, Frankfurt am Main, Germany). PCRs were performed using the Q5 polymerase (Q5 Hot Start High-Fidelity ...
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bioRxiv - Immunology 2022Quote: ... Libraries for bulk RNA sequencing were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, cat. #E7490 and #E7760). The input RNA and the resulting libraries were analyzed with Agilent 4200 Tapestation for quality assessment ...
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bioRxiv - Immunology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Paired-end libraries of 2 x 150 bp fragments with an insert size of 330 bp were prepared with NEBNext® Ultra™ II DNA Library Prep Kits (New England Biolabs, Ipswich, MA, USA), and sequencing was performed on a HiSeq4000 Illumina sequencer (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The constructs M1 and M2 were used as templates to generate higher-order mutants by site-directed mutagenesis PCR (NEB, Q5 Site-Directed Mutagenesis Kit) using the primers listed in Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded RNA-Seq libraries were prepared with 200 ng of total RNA with RIN > 8 using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Poly A+ RNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation and first and second cDNA strand synthesis ...
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bioRxiv - Immunology 2023Quote: ... dual unique indexed libraries were made using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Manufacturer protocol was modified by diluting adapter 1:30 and using 3μL of this dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing of RNA samples was performed by Azenta Life Sciences (Burlington, Massachusetts, US) with NEBNext®Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, Massachusetts, US) for library preparation.
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bioRxiv - Genomics 2023Quote: ... 1 µg of the resulting DNA was used for library preparation using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB cat. E7103). Library PCRs were supplemented with 1× EvaGreen (Jena Bioscience cat ...
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bioRxiv - Biophysics 2023Quote: ... The mutation was introduced with an oligonucleotide designed to alter codon Arg90 (CGC) to Ser (AGC) using a Q5TM site-directed mutagenesis kit (#EO552, #EO554S, New England BioLabs GmbH, Frankfurt am Main, Germany). The successful introduction of mutations was confirmed by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting mRNA material was used to prepare the libraries with the use of NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Cat No. E7760S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... All remaining steps for library construction were used according to the NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Library preparation for gene expression analysis was performed with the NEBNext® Poly(A) mRNA magnetic isolation module and NEBNext® UltraTM II Directional mRNA kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions with 1μg total RNA as input material ...
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bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries for good quality samples (total DNA amount > 20 ng) were prepared with the standard NEBNext®Ultra™ II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA), while double stranded libraries for the samples extracted in the ancient DNA laboratory were prepared following the Meyer and Kircher protocol67 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and sequenced it as a 400- 600bp insert Illumina® library using the NEBNext Ultra II® Library Preparation kit (New England Biolabs® [NEB], Ipswich, MA USA) on an Illumina® NovaSeq lane (Novogene® ...
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bioRxiv - Genomics 2022Quote: ... Capture and removal of CpG methylated host DNA from samples were carried out using the NEBNext® Microbiome DNA Enrichment Kit (NEB, New England Biolabs, Ipswich, MA), following the manufacturer’s recommendations with modifications (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: 1000 ng per sample was then processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat # E7760L; New England Biolabs, Ipswich, USA; protocol v. v3.1_5/20) including PolyA selection ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Cell Biology 2021Quote: ... 80ng of genomic DNA were fragmented to around 170bp followed by End Pair and A tailing with the End Pair mix (NEB Next Ultra DNA library preparation kit). After ligation of truncated TruSeq adapters with the NEB Next Ultra Ligation Module ...
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bioRxiv - Plant Biology 2022Quote: ... Paired end libraries for Illumina sequencing were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Inc, MA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... All libraries were constructed according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). The libraries were quantified by Qubit 2.0 and their quality was assessed by TapeStation D1000 ScreenTape (Agilent Technologies Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... then quantified with the NEBNext® Library Quant Kit for Illumina® using qPCR on a StepOne Plus real-time PCR platform (New England Biolabs® Inc., MA, USA). Following qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One microgram of mRNA was used as a template for first-strand cDNA synthesis using NEBNext® Poly(A) kit (NEB #E7490, New England Biolabs, Inc.) and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760 ...