Labshake search
Citations for New England Biolabs :
9301 - 9350 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 1 μg RNA was used to generate a pair-end sequencing library using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to track sequences to each sample ...
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bioRxiv - Microbiology 2020Quote: ... and further ligated with illumina adaptor sequences using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). The prepared libraries were purified using AMPure XP beads and sequenced as 2 × 250 bp on MiSeq Reagent Kit v2 (illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... GAL4-Creb5(1-128)T59/T61A and GAL4-Creb5(1-128)C18/C23S were generated by inducing point mutations in the GAL4-Creb5(1-128) vector using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat#: E0554S). The control vector GAL4(1-147 ...
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bioRxiv - Molecular Biology 2021Quote: Variants of concern (VOC) of SARS-CoV-2 were created with Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: ... 3 μg of RNA from each species was used to prepare sequencing libraries using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-C library was prepared on beads using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and NEBNext® Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima Hi-C kit ...
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bioRxiv - Molecular Biology 2020Quote: A construct for the deletion of a 9,478 bp region containing genes putatively involved in arsenic resistance was similarly constructed using the NEBuilder assembly kit (New England Biolabs, Ispwich, MA, USA). Genomic DNA on the 5’ flank of the region was amplified using primers AP34 and AP35 ...
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bioRxiv - Immunology 2021Quote: ... and converted to cDNA with ProtoScript® II First Strand cDNA Synthesis Kit (cat. n° E6560L, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturing specifications ...
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bioRxiv - Immunology 2021Quote: ... and converted to cDNA with ProtoScript® II First Strand cDNA Synthesis Kit (cat. n° E6560L, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturing specifications ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (for old herbarium specimens) or the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina®” (for more recent specimens; New England BioLabs, Ipswich, USA). In both cases ...
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bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids expressing cysteine substitution mutant derivatives of pMTD372 and pMTD607 were generated using the Q5 Site-Directed Mutagenesis Kit (NEB catalog number E0554S).
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bioRxiv - Cancer Biology 2019Quote: ... Libraries for whole genome sequencing were prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Inc., Ipswich, MA). Libraries were sequenced on a NovaSeq S1 flow cell (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the eluted RNA was input to the library prep using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).
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bioRxiv - Cancer Biology 2022Quote: ... and bar-coded libraries were constructed using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and sequenced single-end (1×75 ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic localization of BioTAP-Pcl and -Jarid was determined by high-throughput DNA sequencing of libraries generated from BioTAP-XL purified genomic DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, catalog no. E7645) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The sheared DNA was prepared for sequencing library preparation with the NEBNext Ultra II Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Strand-specific cDNA libraries were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Sequencing was performed using NovaSeq6000 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of RNA was used to create indexed cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs, Ipswich, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Products were visualized on 2% agarose gels and successful amplification reactions were prepared for Sanger sequencing with Exo-CIP™ Rapid PCR Cleanup Kit according to the manufacturer’s protocol (New England Biolabs: E1050S, Ipswich, MA). Samples were then sequenced with amplification primers (Eurofins Genomics Services ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared using NEBNext FFPE DNA Repair Mix in combination with the NEBNext Ultra II DNA Library Prep kits for Illumina (New England Biolabs, Ipswich, MA, USA) per manufacturer recommendations with custom inline barcodes as described in Moore et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England BioLabs® Inc., USA). For sequencing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and used to produce RNA-seq libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: All plasmids were constructed through Gibson assembly (Gibson et al., 2009) using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA, USA). Primers used in this study were purchased from Eurofins Genomics (Ebersberg ...
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bioRxiv - Genomics 2019Quote: ... Short-insert of 250 bp paired-end libraries were prepared for both individuals using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Biolabs) and 10 PCR cycles ...
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bioRxiv - Cell Biology 2019Quote: ... and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Microbiology 2019Quote: ... One hundred ng RNA from each sample were prepared for cDNA sequencing using NebNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The reaction was topped up to 200μL with DEPC treated water and a phenol chloroform extract performed (as before except the pellet was resuspended in 17.5μL DEPC treated water. The SR RT primer (NEB small RNA kit for Illumina) was hybridized to the 3’ adapter and the 5’ adapter ligated by adding 2μL T4 RNA ligase buffer (NEB) ...
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bioRxiv - Physiology 2019Quote: ... the culture sample was diluted with the DNA/RNA protection reagent of the Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA), followed mechanical lysis with ZR BashingBead™ Lysis Tube (0.5mm ...
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bioRxiv - Physiology 2020Quote: ... Sequencing library was prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs Inc., Cat # E7770S) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... sgRNAs were synthesised directly from gBlock® (IDT) templates containing the T7 promoter using the HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs®) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Digoxigenin (DIG)-labelled anti-sense and sense RNA probes corresponding to GPA2 and GPB5 subunits were synthesized using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Whitby, ON, Canada). Fluorescence in situ hybridization (FISH ...
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bioRxiv - Genomics 2019Quote: ... Library preparation for sequencing was performed using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645, New England Biolabs NEB). Samples were sequenced paired-end ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell pellets were suspended in 800 µL of DNA/RNA protecting buffer from the Monarch Total RNA MiniPrep kit (New England Biolabs, Ipswich, MA, USA), transferred into the ZR S6012-50 Bashing beads lysis tubes (Zymo Research ...
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bioRxiv - Synthetic Biology 2019Quote: ... 80 ng of RNA of each sample was converted into cDNA using the LunaScript RT SuperMix kit (New England Biolabs, Ipswich, MA, USA). qPCR experiments were performed with the Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA libraries were prepared using NEBNext® Ultra™ RNA Library Prep Kits for Illumina® (New England BioLabs, Ipswich, MA, USA) according to the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-seq library prep was done with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs cat. no. E7420) per the manufacturer’s standard protocol.
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bioRxiv - Evolutionary Biology 2020Quote: ... dual-indexed Illumina genomic libraries were prepared for each DNA sample using an insert size of ~350 bp and the Ultra II Library Prep Kit (New England BioLabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: The RNA-Seq libraries were prepared using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswick MA), following manufacture’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons were gel purified and 100 ng of the mutated linearized plasmids were re-ligated by using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, Ipswich, MA, USA), following the manufacturers’ protocol ...
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bioRxiv - Zoology 2020Quote: ... of the reaction was then checked for successful amplification on a 1% agarose with the 1 kb DNA Ladder from New England Biolabs and afterwards the remaining PCR product was purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ipswich, MA). Purified products for S ...
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bioRxiv - Zoology 2021Quote: ... a total of nine RNA libraries were prepared with 3 μg RNA using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... of which 5 μl was processed with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) with previously published modifications to the manufacturers protocol 37 ...
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bioRxiv - Microbiology 2021Quote: ... DNA libraries were prepared in a paired-end layout with NEBNext® Ultra™ II FS DNA Library Prep Kit (New England BioLabs, UK) and sequenced under a 150-bp read length using Illumina HiSeq 4000 systems and HiSeq SBS Kit v4 reagents (Illumina ...
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bioRxiv - Pathology 2021Quote: RT-qPCR was also performed on RNA extracted from the 4 dpi lung samples with NEB Luna Universal One-Step RT-qPCR Kit with SYBR-Green (NEB, Ipswich, MA, USA) to quantify the cytokines IL-6 and IFN-β ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genomic libraries were prepared using the NEBNext Ultra DNA Library Prep Kit with NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, MA, USA) to enable multiplexed sequencing ...
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bioRxiv - Genomics 2021Quote: ... Fragmented gDNA was ligated to NEBNext Adaptor for Illumina using NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, cat. No. E7645). We performed bisulfite conversion twice with ligated libraries (QIAGEN ...
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bioRxiv - Genetics 2020Quote: ... Sheared genome DNA was used to build the library using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (E7370/7335, NEB) and Illumina sequenced by Ribobio in Wuhan ...
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bioRxiv - Genetics 2020Quote: ... 17-25 nt small RNA fractions were isolated by denaturating PAGE and subjected to standard small RNA library preparation using the NEB Next small RNA sequencing Kit (NEB, Frankfurt a.M., Germany). The procedure includes 3′-OH and 5′-monophosphate specific ligation steps and we tried to lower 3′-2′-O-me biases by 18 hours 3′-ligation at 16°C ...
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bioRxiv - Cell Biology 2021Quote: ... two-step RT-qPCR was performed using LunaScript® RT SuperMix Kit (E3010L) for cDNA synthesis and Luna® Universal qPCR Master Mix (NEB #M3003) for RT-qPCR ...