Labshake search
Citations for New England Biolabs :
9551 - 9600 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Metagenomic libraries were prepared from DNA (100 ng) using the NEBNext® Ultra II FS DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) > 100ng input protocol as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... We inserted TFT reporters into BFA0190 by digesting the plasmid with SwaI and inserting TFT components between the LYP1 flanking sequences using isothermal assembly cloning (Hifi Assembly Cloning Kit; New England Biolabs [NEB], Ipswich, MA, USA). The 5’ and 3’ LYP1 flanking sequences in each TFT plasmid contain natural SacI and BglII restriction sites ...
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bioRxiv - Molecular Biology 2022Quote: ... A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...
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bioRxiv - Genomics 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
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bioRxiv - Genetics 2022Quote: ... with insert-specific primers LC186 and 187) between hlh-30 and 3XFLAG-tag segments with HiFi cloning (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs Inc., Ipswich, MA). Lastly ...
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bioRxiv - Cancer Biology 2022Quote: ... CUT&RUN barcoded libraries for Illumina sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) accordingly with Zhu et al.(Zhu et al. ...
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bioRxiv - Plant Biology 2021Quote: ... and phloem tissues were also prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs®) ...
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bioRxiv - Genomics 2020Quote: Genomic DNA purified from one single haploid drone of each species was used to generate one “fragment” library with an insert size of 400 or 450 bp using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA). The prepared fragment libraries were sequenced on an Illumina HiSeq 2500 platform with a read length of 250 bp to produce overlapping paired-end shotgun reads (2 × 250 bp) ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (cat no. E7770, New England Biolabs, Ipswich MA). Single-end sequencing was performed using the Illumina NextSeq500 platform according to standard operating procedures.
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bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Illumina MiSeq adaptors were ligated onto the amplicon library using a NEBNext® DNA MasterMix for Illumina Kit (New England BioLabs, Ipswitch, MA, USA) and sequences were generated using a MiSeq instrument (2 × 250 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for ChIP-seq were constructed using NEBNext® UltraTM DNA Library Prep Kit for Illumina (Cat. #E7370; New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The extracted DNA from water and soil underwent a further purification step using Monarch®PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA). DNA Library preparation was conducted using Native barcoding genomic DNA Kit SQK-LSK 109 combined with EXP-NBD104 (Oxford Nanopore Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... 100 ng total RNA were used as input for ribosomal RNA (rRNA) depletion using the NEBNext rRNA depletion kit (New England BioLabs inc., product code: E6350) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... DNA samples were purified before and after PCR with the Monarch® PCR and DNA Clean-Up Kit (New England Biolabs, Ipswitch, MA, USA). Individual sample libraries were pooled ...
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bioRxiv - Bioengineering 2022Quote: ... The library was prepared by a Ribo-Zero protocol (250-300 bp insert strand specific library with rRNA removal using NEB Ribo- Zero Magnetic Kit). Paired-end sequencing produced 150 bp reads ...
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bioRxiv - Plant Biology 2019Quote: ... Sequencing libraries were constructed from 1 µg of total RNA using NEBNext Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... Sequencing libraries were prepared from the sheared DNA using the NEBNext® DNA library prep kit for Illumina (New England BioLabs, Ipswich, Massachusetts, USA). Size selection was performed using an E-gel precast agarose system ...
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bioRxiv - Molecular Biology 2019Quote: Libraries for next generation sequencing were prepared by using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645) following the protocol supplied with the kit and individual samples were indexed during the PCR step with NEBNext® Multiplex Oligos for Illumina® ...
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bioRxiv - Evolutionary Biology 2020Quote: ... leprae positive faecal and necropsy samples (Supplementary Information Table 1) were converted into dual-indexed libraries using the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA)57,58 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK or equivalent from Illumina, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μg RNAs for each sample were used for library construction using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA, cat. no. E7530) according to the instruction and RNA-Seq on Illumina Hiseq 2500 platform.
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bioRxiv - Developmental Biology 2020Quote: ... digested pCFD6 backbone to generate UAS-t::gRNA-twi4x using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Cat. No: R5520S), following the instructions provided by the manufacturers.
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bioRxiv - Molecular Biology 2020Quote: ... The DAP gDNA library was prepared using the kit from NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB #E6040S/L). ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A ...
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bioRxiv - Genomics 2021Quote: Whole Genome DNA libraries with average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Plant Biology 2021Quote: ... cablin seedlings and were introduced into the expression vector pET-28a (+) between the restriction sites of NdeI and BamHI using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, Catalog number: E5520S), in each case generating a fusion gene that encoded a “tag” of HIS6 residues at the N-terminus for expression in E ...
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bioRxiv - Microbiology 2020Quote: ... samples were treated using a Pan-Prokaryote riboPOOL™ kit (siTOOLs Biotech Gmbh, Planegg, Germany) coupled with streptavidin magnetic beads (New England Biolabs, Ipswich, MA, USA). mRNA was eluted in 50 μl of nuclease-free water and then cleaned and concentrated using a Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... Three recB fragments were directly assembled into pET28c vector in frame with the N-terminal 6xHis tag employing NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Inc., Ipswich, MA, USA). Wild type RecC encoding gene was individually sub-cloned into pET28c vector in frame with the N-terminal 6xHis tag ...
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bioRxiv - Genomics 2021Quote: ... cDNA libraries were synthesized using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (Both: New England Biolabs, Ipswich, Massachusetts) and subsequently sequenced in a paired-end mode with 75bp read length on NextSeq500 (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg gDNA was then used to prepare the gDNA sample library using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and sequencing libraries were constructed using a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, E7420L) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Cell Biology 2022Quote: ... We created DNA libraries with an average library size of 320 bp using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Library preparation and sequencing were prepared with Illumina-compatible NEBNext® Ultra™ II using Directional RNA Library Prep Kit (New England BioLabs, MA, USA). Viral total RNA was taken for fragmentation and priming ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We used a standard miniaturized protocol to prepare DNA libraries using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA))56 ...
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RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: ... and pELS53 [Peft-3::Cas9 + rtel-1 sgRNA] plasmids were generated for this study by modifying the pDD162 plasmid (Dickinson, 2013) using Q5 Site Directed Mutagenesis Kit (NEB, Cat# E0554S, Ipswich, MA).
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bioRxiv - Microbiology 2022Quote: ... with duplicate sample in each group) was depleted of bacterial rRNA using NEBNext® rRNA depletion kit (Cat. no. E7850X, New England Biolabs, Ipswich, MA,USA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... and reversely transcribed into cDNA from 1 μg of mRNA template using the M-MuLV cDNA Synthesis Kit (New England Biolabs, Frankfurt am Main, Germany) according to the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated from 1 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Whole mRNAseq libraries were generated using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). The clustering of index-coded samples was then performed on a cBot Cluster Generation System ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were generated from 1 µg total RNA per sample using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... dsRNA was synthesized from T7-linked DNA using the HI Scribe™ T7 High Yield RNA Synthesis Kit (New England, Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... strand-specific total-transcriptome RNA sequencing libraries were prepared by incorporating dUTPs in the second-strand synthesis using NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA). The isolated cell RNA-seq libraries were prepared using the Ovation® SoLo® RNA-Seq library preparation kit (Tecan ...
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bioRxiv - Developmental Biology 2022Quote: The RNA-seq libraries were prepared from 100 μg of total RNA per genotype with NEBNext Ultra II RNA Library Prep Kit mRNA Isolation Module (NEB, Cat# E7775S, E7490S, E7500S). Quality control and sequencing of the RNA-seq libraries were performed by GENEWIZ ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared from the enriched mRNAs using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA). The library concentration was determined in a Qubit3 Fluorometer using the Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from agar plate-grown seedlings by acid guanidinium thiocyanate-phenol-chloroform-based extraction and purified from the aqueous phase using the Monarch RNA Clean Up Kit (New England Biolabs, Ipswich, MA, USA; NEB). Genomic DNA in the samples was removed using TURBO™ DNase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat. No. E7645) and the NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Cat ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis thaliana RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Preparation Kit (New England BioLabs, MA, USA). Five hundred ng of total RNA was taken for mRNA isolation ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA-seq libraries were constructed using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Finally ...