Labshake search
Citations for New England Biolabs :
9401 - 9450 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... A second PCR purification was performed prior to insertion of the Ultramers into the vector by DNA assembly with the NEBuilder HiFi DNA Assembly Kit (NEB, Cat. No. E2621). Next ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was cloned using either classical restriction and ligation ([55]; restriction enzymes, T4 DNA ligase and Quick Ligation kit from New England Biolabs, Ipswich, Massachusetts, USA) or isothermal assembly ([56] ...
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bioRxiv - Plant Biology 2024Quote: 5 μl of ribosomal depleted RNA from each sample were used to prepare 64 individually barcoded RNA-seq libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from 30-35 sets of MTs isolated from 5-7-day old female flies in five independent biological replicates using the Monarch Total RNA Miniprep kit (New England BioLabs, Whitby, ON, Canada). Total RNA was quantified using a UV spectrophotometer (Synergy 2 microplate reader ...
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bioRxiv - Physiology 2024Quote: ... Samples were then thawed at room temperature and total RNA was isolated using the Monarch Total RNA Miniprep Kit following manufacturers protocol with an on-column DNase treatment to remove genomic DNA (New England Biolabs, Whitby, ON, Canada). Purified total RNA samples were subsequently aliquoted onto a Take3 micro-volume plate and quantified on a Synergy Multi-Mode Microplate Reader (BioTek ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit according to the manufacturer’s recommendation for Illumina® (NEB, USA). The library quality was assessed using the Agilent 2100 Bioanalyzer system ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated with the NEBnext® UltraTM DNA Library Prep kit for Illumina® (New England Biolabs, Inc., Ipswich, MA, USA). After quality assessment via Qubit (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... 3 ug RNA was treated with Promega RQ1 DNase (M6101) then cleaned up with the Monarch® RNA Cleanup Kit (NEB Biolabs, T2050S) and eluted in 30 µl dH2O.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were generated at the Novogene Bioinformatics Institute using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... Strand-specific RNA-seq libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® sequencing (E7760S) according to the manufacturer’s protocol (New England Biolabs, MA USA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were generated from 500 ng of total RNA using NEBNext Ultra II Directional Poly-A RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA; #E7760). cDNA library quality and quantity were assessed by TapeStation 2200 and Qubit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq and MNase-seq libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat. no. E7645S) and Agencourt Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: Library preparation for RNA sequencing was performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA, Catalog #: E7530L), strictly adhering to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Amplified genes were purified by agarose gel electrophoresis and extraction with the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA, USA). Both the synthesized genes and PCR amplified genes were then inserted into a doubly digested BamHI/XhoI pET28b(+ ...
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bioRxiv - Immunology 2024Quote: ... were prepared and sequenced at Azenta (South Plainfield, NJ) using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The sequencing libraries were validated on the Agilent TapeStation ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared dual indexed libraries according to the instruction manual using the NEBNext Ultra II DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, New England Biolabs, Ipswich, Massachusetts, USA) and following the recommended conditions of bead-based size selection according to distribution of DNA fragments per sample ...
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bioRxiv - Genetics 2023Quote: Twenty nonfunctional 9 base pair barcodes “CellTags” were subcloned into pLentiV_Blast using Q5® Site-Directed Mutagenesis kit (New England Biolabs; catalog no. E0552) (Biddy et al. ...
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bioRxiv - Genomics 2024Quote: ... Metagenomic libraries were generated with the NEBNext Ultra DNA Library Preparation kit following the manufacturer’s instructions (New England Biolabs, Ipswich, MA, United States) and paired-end sequenced (2 x 150 bp ...
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bioRxiv - Biochemistry 2024Quote: N-term-His-ELMO1C438A construct was generated from N-term-His-ELMO1 construct obtained above using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S), with the following primers.
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bioRxiv - Biochemistry 2024Quote: ... B2R phosphorylation site mutants mentioned in the manuscript were generated by site-directed mutagenesis using Q5 Site-directed mutagenesis kit (NEB, Cat. No. E0554). All DNA constructs used in this study were verified by sequencing from Macrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... after which the cells were washed twice with DPBS and total RNA was extracted using the Monarch® Total RNA Miniprep Kit (NEB, Ipswich, MA). Total RNA was stored at −80°C until sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... beads and library preparation was carried out with the Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The Illumina NovaSeq6000 platform was used for library sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... RNA Library preparations for Synpo-related transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Polyadenylated RNA enrichment and library preparation were conducted using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB, #E7490S and #E7760S), respectively ...
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bioRxiv - Immunology 2022Quote: ... the MPXVac-097 vaccine candidate construct encoding five monkeypox antigens in tandem was in vitro transcribed from linear DNA template using HiScribe T7 ARCA mRNA kit (NEB, Cat. No. E2060S) with 50% replacement of uridine by N1-methyl-pseudouridine (Tirlink ...
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bioRxiv - Microbiology 2023Quote: ... each of the upstream and downstream fragments was ligated with the pKKPH fragment using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Japan, Tokyo, Japan). To insert pKKPH-p49 to the genome of ΔpyrFHM13 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA extraction was performed using TRI reagent (Cosmo Bio Co., Ltd., Tokyo, Japan) and the Monarch Total RNA Miniprep Kit (New England BioLabs Inc., Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were sequenced on an Illumina NovaSeq 6000 sequencer.
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bioRxiv - Microbiology 2022Quote: ... A total amount of 1 μg DNA per sample was used as input material to prepare libraries that were generated using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The upstream and downstream PCR products were designed to have an overlapping region of sequence to promote 3-part Gibson assembly (NEB Gibson assembly kit) and primers 5b and 4 had extensions to anneal to vector pMP62 ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 – 1 μg DNA template were in vitro transcribed using HiScribe® T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA), following manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used to generate sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following polyA selection ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and quantitative PCR were performed in one step using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). The primers used are listed in supplemental table (Table ...
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bioRxiv - Genomics 2023Quote: ... were used to remove rRNA from total RNA and libraries prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, New England Biolabs). The libraries were sequenced on an Illumina NovaSeq S4 (Illumina ...
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bioRxiv - Immunology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). The Hifi assembly products were Ampure bead purified and eluted into 20 µL of H2O ...
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bioRxiv - Genomics 2023Quote: ... and single colonies were picked 1 day later to grow up and extract plasmid DNA using a Monarch Plasmid Miniprep Kit (New England Biolabs, Cat. No. T1010L). Extracted plasmid DNA was sequence confirmed via long-read Nanopore sequencing (Primordium Labs ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... NEB Ultra II directional RNA-sequencing Library Prep kit protocol was used to prepare libraries for total RNA-Seq (NEB, catalog no. E7760L). An initial concentration of 500ng of total RNA was taken for the assay ...
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bioRxiv - Biochemistry 2023Quote: ... The primers listed in Table 1 and 2 were designed to introduce point mutations in the WT variants using the Q5® site-directed mutagenesis kit (New England BioLabs, Ipswich, USA). All variants were verified by Sanger sequencing (Microsynth ...
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bioRxiv - Plant Biology 2023Quote: ... Sheared DNA was then used to generate barcoded libraries utilizing NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA). Libraries produced followed the NEBNext Ultra II Version 5 protocol with size selection on DNA fragments at 300-400bp range but were adjusted by halving the amount of reagents and DNA used to save on supplies and resources ...
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bioRxiv - Cancer Biology 2023Quote: ... and bar-coded libraries were constructed using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and sequenced single-end (1X75 ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” combined with “NEB Ultra II polyA mRNA magnetic isolation” for mRNA enrichment (New England Biolabs, Ipswich, MA, USA). Libraries were pooled and sequenced (single-end ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were prepared for each sample at the University of Texas Genomic Sequencing Facility using the NEBNext Small RNA library preparation kit (New England Biolabs; Ipswich, MA, USA). Libraries were enriched with 14 cycles of PCR and the final product was size selected for 105–155 bp using a 3% gel cassette on the Blue Pippin (Sage Science ...
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bioRxiv - Genetics 2023Quote: ... COL3A1-5’ UTR fragment was created from the reference allele (TT) PCR product using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Massachusetts, USA). The sequence of primers carrying out the alternative allele ...
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bioRxiv - Genomics 2023Quote: ... The cDNA libraries were constructed from 1 ug input RNA using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB), following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: All PolD mutants (Supplementary table 3) were generated using pLB047 and a Q5 Site-Directed mutagenesis kit (E0554 New England Biolabs Inc., Ipswich MA) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Molecular Biology 2023Quote: ... the DNA pellet was resuspended in 0.1 X TE and used for DNA library generation with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit) ...
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bioRxiv - Genomics 2023Quote: ... Then the enriched mRNA was sheared into short fragments using fragmentation buffer and reversely transcribed into cDNA by Next Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). CDNA libraries were sequenced on Illumina Novaseq6000 platform by Gene Denovo Biotechnology Co ...
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bioRxiv - Immunology 2023Quote: ... dual unique indexed libraries for sequencing on Illumina platforms were generated using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). The manufacturer’s protocol was modified by diluting adapters 1:30 and using 3 µL of this dilution ...