Labshake search
Citations for New England Biolabs :
9201 - 9250 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and the sequencing library was generated using the NEBNext® Ultra™II Directional RNA Library Prep Kit (New England Biolabs, E7760). RNA-seq was performed on NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Site directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instruction (New England BioLabs Inc. (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Illumina compatible cDNA libraries generated for polyA selected mRNA using NEB next ultra RNA library preparation kit Cat#E7530L (New England Biolabs Inc, MA). The cDNA libraries were purified using AmpureXP beads ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 ng of total RNA was used for RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, cat# E3005L) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and the linear backbone vector (5098 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S). The second product from the digestion (2878 base pairs ...
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bioRxiv - Bioengineering 2024Quote: The two fragments from pCpf1b-sp2 plasmid and the gRNA cassette obtained above was assembled by using Golden Gate Assembly (Golden Gate Assembly Kit, New England BioLabs, and E1601S). The cycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... input and immunoprecipitated DNA were prepared into multiplexed libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). For the RNA-seq library ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fully homogenized tissue was then used for DNA purification using the “Protocol for Extraction and Purification of Genomic DNA from Tissues” protocol for extraction and purification of genomic DNA from tissues in the Monarch® Genomic DNA Purification Kit (New England Biolabs T3010). DNA extract quality was assessed for quality (260:230 nm and 260:280 nm wavelengths ...
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bioRxiv - Genomics 2024Quote: Each cDNA library was built from 300 ng total RNA with ERCC spike-ins (Thermo cat. #4456740) using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB cat. #E7760), specifically the protocol for use with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg total RNA was used as input to NEBNext Poly(A) mRNA Magnetic Isolation Module for NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Cat #E7775), following the manufacturer’s protocol and using 7 PCR cycles for library amplification ...
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bioRxiv - Neuroscience 2024Quote: ... Isolated RNA from brain regions and purified microglia was converted to cDNA and prepared for Real Time quantitative PCR (RT-qPCR) reactions using the LunaScript® RT SuperMix Kit (New England Biolabs E3010) and Luna® Universal qPCR Master Mix (New England Biolabs M3003) ...
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bioRxiv - Molecular Biology 2024Quote: ... a two-step process was used in which cDNA was first synthesized with oligo dT priming using the ProtoScript II First-Strand cDNA Synthesis kit (New England BioLabs, Ipswich, MA). To denature the template RNA ...
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bioRxiv - Biochemistry 2024Quote: ... The concentration of adapter-labelled DNA fragments in each sample was measured via qPCR using NEBNext® Library Quant Kit for Illumina® (NEB). The samples were then pooled together to an equimolar concentration and checked on a Tapestation (Agilent).
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bioRxiv - Plant Biology 2024Quote: ... cDNA libraries were constructed using a NEBNext Ultra II RNA Library Prep Kit with Sample Purification Beads (New England Biolabs, Tokyo, Japan), a NEBNext Poly(A ...
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bioRxiv - Biochemistry 2024Quote: ... DNA digestion was performed with DNase I and RNA was reverse-transcribed into cDNA using LunaScriptTM RT SuperMix kit (New England Biolabs, Cat#E3010L). qPCR was performed using the StepOnePlusTM Real-Time PCR System with SYBR green as the detection method ...
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bioRxiv - Bioengineering 2024Quote: ... Reactions were incubated at 16°C for 16 hours and purified with Monarch® RNA Cleanup Kits (New England Biolabs, Cat# T2030) according to manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... with library preparation conducted using the NEBNext® Ultra™ IIDNA Library Prep Kit (from New England BioLabs Inc., Cat No. E7645). Ethical approval was obtained from the medical research ethics committees of the Ministry of Health ...
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bioRxiv - Evolutionary Biology 2024Quote: The RNA prepared as described above was sent to Macrogen Japan for library preparation and cDNA sequencing as follows: ribosomal RNA (rRNA) was removed from each sample using the NEBNext Bacteria rRNA Depletion Kit (New England BioLabs, Ipswich, MA), and library preparation was performed using the TruSeq Stranded Total RNA Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L, New England BioLabs ® Inc., Ipswich, MA, USA) as described previously (6 ...
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bioRxiv - Cell Biology 2024Quote: ... Purified RNA was treated with PNK4 before being used for cDNA synthesis using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (NEB). Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and the eluate was pooled together and used to construct DNA libraries for Illumina deep sequencing using an NEB DNA Library Prep Kit (NEB; cat # E7645S). The prepared DNA libraries were stored at –80°C before being sequenced at the Tufts University Core Facilities (US).
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bioRxiv - Microbiology 2024Quote: ... For Nanopore sequencing high-molecular weight DNA was isolated from a separately cultivated batch with the Monarch Genomic DNA Purification kit (New England Biolabs, Frankfurt, Germany) and library preparation was performed with 1.5 µg using the ligation sequencing kit 1D (SQK-NBD114.24 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of the RNA were sent to Azenta Life Sciences for genome-wide mRNA sequencing using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, MA) for NovaSeq (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... About 200ng of DNA was taken and preceded for library preparation using NEBNext Ultra II DNA kit (E7645, New England Biolabs, Massachusetts, United States) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... prior to library preparation with NeBNext®Ultra™II Directional RNA Library Prep Kit for Illumina (both New England BioLabs, Ipswich, USA) using an RNA input of 100 ng per sample (New England BioLabs ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were constructed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to the protocol for ribosome depleted RNA and with a 11 min RNA fragmentation step (NEB - catalogue no. E7760). Library PCRs were supplemented with 2x SYBR dye (Sigma – catalogue no ...
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bioRxiv - Immunology 2021Quote: ... Libraries from purified CD14+ monocytes RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA), which allows for lower input concentrations of RNA (10ng) ...
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bioRxiv - Cell Biology 2020Quote: ... The coding sequence for Dendra2 was amplified from a plasmid clone and inserted into this backbone using the Gibson Assembly® Cloning Kit (New England Biolabs, Frankfurt, Germany); the primer sequences are available upon request ...
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bioRxiv - Developmental Biology 2021Quote: ... by in vitro transcription from v5-hPMLVI or v5-hPMLVI (K160, 490R) using a HiScribeTM T7 ARCA mRNA Kit with tailing (New England Biolabs, Ipswich, MA, USA) and a MEGAclearTM Transcription Clean-Up Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from uninfected and FV3-infected Xela DS2 cells at 24 h post-infection (hpi) and 72 hpi using the Monarch Total RNA Minipreps kit (New England Biolabs, Ipswich, United States) and genomic DNA was removed using on-column DNase I digestion ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples were sheared on a Covaris E-220 Focused-ultrasonicator and sheared DNA was then used to prepare Illumina sequencing libraries with the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs. France) and the NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) ...
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bioRxiv - Molecular Biology 2021Quote: Extraction of total RNA from HCT116 +TetOsTIR cells was performed by using Monarch® Total RNA Miniprep Kit (New England Biolabs, Inc, #T2010S). cDNA library construction using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human (Illumina #RS-122-2201 ...
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bioRxiv - Molecular Biology 2021Quote: ... Complementary DNA (cDNA) was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, Ipswich, MA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase (FLuc) RNA served as the spike in control and was generated using HiScribe T7 Quick RNA Synthesis kit (NEB, Ipswich, MA, USA) using linearized plasmid DNA encoding FLuc gene as template ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and single bands of the expected size were excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs, MA, USA). Purified PCR products were cloned into pCR-XL-TOPO™ vector using a TOPO™ XL PCR Cloning Kit following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina sequencing libraries were constructed from sheared DNA samples using an NEBNext Ultra II DNA library preparation kit (New England Biolabs, Ipswich, MA, USA) with dual indexes for multiplexing following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... libraries were prepared either directly in-house with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs), or at the sequencing company Novogene (Cambridge ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified Mmp13 promoter sequences and digested pGL3 were purified using GeneJET PCR Purification Kit and cloned using NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEB, Ipswich, MA, USA). Mutations in Mmp13 promoter SNPs were generated through site-specific mutagenesis PCR with the mutations in the primers (Ho et al. ...
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bioRxiv - Genomics 2020Quote: ... Multiple adapters were used for pooling libraries (KAPA Single-Indexed Adapter Kit KK8700 and NEBNext® Multiplex Oligos for Illumina NEB #E7535S/L). The DNA samples were sequenced using the Illumina NextSeq 500 platform and 150-nucleotide single-end reads were generated ...
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bioRxiv - Microbiology 2022Quote: cDNA libraries were generated with the NEBNext Ultra II Directional RNA Library Prep with rRNA Depletion Kit (New England BioLabs catalog no. E7760) by the High Throughput Genomics Core at the University of Utah ...
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bioRxiv - Genomics 2022Quote: ... Capture and removal of CpG methylated host DNA from samples were carried out using the NEBNext® Microbiome DNA Enrichment Kit (NEB, New England Biolabs, Ipswich, MA), following the manufacturer’s recommendations with modifications (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645L) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Poly(A) mRNA was prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs) and 2 × 100bp paired-end sequencing at a depth of 80-100 million reads per sample was performed using the Illumina Novaseq 6000 platform by the University of Toronto Donelly Sequencing Centre ...
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bioRxiv - Molecular Biology 2021Quote: ... The rRNA-depleted RNAs were then subjected to RNA-seq library construction using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, #E7765). The final RNA-seq libraries were amplified with 7 – 8 PCR cycles.
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bioRxiv - Molecular Biology 2021Quote: ... coli using the Qiagen Miniprep kit (Hilden, Germany) and performing double restriction enzyme digests using EcoRI and HindIII purchased from New England Biosciences (NEB, Ipswich, MA, USA), using the manufacturer’s recommended procedures.
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bioRxiv - Genomics 2022Quote: ... The purified A-tailed DNA was ligated with adaptors from the Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore Technologies) and the NEBNext® Quick Ligation Module (NEB, Cat. E6056). The purified ligation products were used as the constructed sequencing library ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 1000 ng of RNA was utilized for library preparation with the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...