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Citations for New England Biolabs :
9251 - 9300 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 3.0μg RNA per sample was used to prepare the sequencing libraries using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). All above libraries were constructed and sequenced by Novogene Corporation (Beijing ...
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bioRxiv - Genomics 2020Quote: ... 75 ng for 37PF and 100 ng for 362YU3) were fragmented using the NEBNext® Ultra™ II FS DNA kit (New England Biolabs, USA) at 37°C for 20 minutes (resulting in DNA fragments with an average size of 350 bp) ...
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bioRxiv - Genomics 2020Quote: ... DNA was then purified and indexed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® per the manufacturer’s instructions (New England Biolabs, Inc). Libraries were sequenced on a HiSeq4000 with paired end 150bp chemistry at the Michigan State Genomics Core Facility (East Lansing ...
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bioRxiv - Immunology 2021Quote: Adherent Xela DS2 cells and any detached cells in suspension were harvested for total RNA isolation using the Monarch Total RNA Minipreps kit (New England Biolabs, Ipswich, United States) according to the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2021Quote: ... mRNA was generated from the T7 promoter in the linearized plasmids with the HiScribe T7 ARCA mRNA kit with tailing (NEB #E2060S, Ipswich, MA). We would typically use half of a kit (10 reactions ...
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bioRxiv - Molecular Biology 2019Quote: M6A and m1A immunoprecipitationwere adapted from the protocols provided in the EpiMark® N6-Methyladenosine Enrichment Kit (NEB #E1610S, New England BioLabs Inc) and that previously described (28) ...
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bioRxiv - Molecular Biology 2020Quote: ... The Npac mutant plasmid was generated according to the manual of Q5® Site-Directed Mutagenesis Kit (E0554S, New England Biolabs. Ipswich, MA). The plasmid of full-length Npac cDNA inserted in pCAG-Neo vector was used as PCR template ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of total RNA from various tissues was used to construct RNA-seq library and sequence on the Illumina HiSeq X-10 platform following the protocol of NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Ipswich, MA, USA).
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bioRxiv - Genomics 2019Quote: ... 3 μg RNA was used to generate sequencing library by using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). PCR was carried out using Phusion High-Fidelity DNA polymerase ...
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bioRxiv - Genomics 2020Quote: ... RNAseq was performed with 100 bp paired-end reads using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... asparagine 36 and 484 were replaced by glutamine in N-glycosylation sites through PCR using the Gibson Assembly® Cloning Kit (New England BioLabs, Frankfurt, Germany) with primers PDI1Asn36mut-1 and PDI1Asn36mut-2 for the mutation N36Q and primers PDI1Asn484mut-1 and PDI1Asn484mut-2 for the mutation N484Q ...
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bioRxiv - Physiology 2019Quote: ... Total RNA was then isolated from four-day old whole female mosquitoes injected with dsCAPAr or dsARG using the Monarch Total RNA Miniprep Kit (New England Biolabs, Whitby, ON, Canada) and used as template (500ng ...
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bioRxiv - Microbiology 2019Quote: Mutant strains of A12 were engineered by double-crossover recombination using linear DNA assembled through a Gibson Assembly® kit (New England Biolabs, Beverly, MA) (32) ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was done using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to manufacturer instructions with 4 cycles of PCR amplification ...
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bioRxiv - Genetics 2021Quote: RNAseq libraries were prepared with the “protocol for library preparation of Intact RNA using NEBNext rRNA depletion kit (Bacteria) (NEB#E7850, NEB#78860) and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760 ...
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bioRxiv - Genomics 2021Quote: ... A total of eight cDNA libraries (four per experimental condition) were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (New England Biolabs, USA). The libraries were sequenced using Illumina HiSeq 2500 (paired-end reads with 150 bp length) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately one microgram of total genomic DNA from each sample was used for ~400-bp library construction with the NEBNext DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and Covaris-based fragmentation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to evaporate the samples to enable miniaturized mNGS library preparation with the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Beverly, MA, USA). Library preparation was performed per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... was prepared with the Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore) and the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (BioLabs, E7180S) using the Nanopore Oxford standard protocol for ligation sequencing.
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bioRxiv - Immunology 2021Quote: ... Libraries from purified CD14+ monocytes RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA), which allows for lower input concentrations of RNA (10ng) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was used to prepare 48 separate cDNA libraries to produce 100 base pair paired end reads using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA). Each library was individually barcoded with NEBNext dual adaptors (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were concentrated and purified with a Monarch® PCR & DNA Cleanup Kit and the entire 8 μl of eluted product was used as a substrate for Illumina sequencing library construction using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) and the protocol provided with the kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were individually barcoded and pooled into a single library with the NEB Next Ultra DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and sequenced on a HiSeq machine (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... The H543R point mutant from HF-GPR133 was generated by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat# E0554S), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from a high-titer ϕHNL01 lysate and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs, Ipswich, MA, USA). Libraries were quantified using a BioAnalyzer (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing libraries were generated from 500 ng of total RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat No. E7530), including polyA selection ...
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bioRxiv - Plant Biology 2021Quote: ... all RNA-seq libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (catalog number E7765; New England Biolabs) using 500 ng of total RNA as starting material ...
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bioRxiv - Plant Biology 2021Quote: ... promoters were inserted into the pH7WG Gateway vector (Department of Plant Systems Biology, Ghent University, Belgium (Karimi et al., 2002)) by the Gibson Assembly kit (New England Biolabs, Ipswich, MA, USA). The tagged PIN7 and PIN4 cDNA entry clones were then recombined with the modified pH7WG destination vector by the Gateway LR reaction (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... and used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), following manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... Amplicons and pCineo plasmid (linearized by NheI/EcoRI digestion) were assembled using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, cat. no. E5520), following the manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... In total 27 libraries (3 species x 9 time points) were prepared according to NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, U.S.A.) followed by paired end sequencing (100bp x 2 ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 1 μg RNA was used as input material for library generation using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB USA), at which point index codes were added to each sample ...
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bioRxiv - Genetics 2021Quote: ... sgRNAs were synthesized directly from gBlock® DNA (IDT) templates containing the T7 promoter using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs E2050) following manufacturer’s instructions for sgRNA synthesis.
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bioRxiv - Microbiology 2021Quote: ... The resulting sheared DNA was used for library preparation using the NEBNext Ultra II Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The amplified mRNA of each sample was used to construct sequencing libraries using NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, USA) according to the instruction manual ...
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bioRxiv - Plant Biology 2021Quote: ... a non-adenylated 3’ adapter was ordered for chemical synthesis from IDT™ and adenylated with the 5’ DNA Adenylation Kit (New England Biolabs® Inc.) (see Supplemental Table 4 for primers and adapters used) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-Seq libraries were generated from 2 µg RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7490 and #E7420). Libraries were quantified using the Agilent DNA 7500 Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed to enrich for sub-nucleosomal fragments using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina as described (NEB, E7645S). The libraries were size-selected and purified with AMPure XP beads ...
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bioRxiv - Cell Biology 2021Quote: pcDNA4/TO-GFP-CLC7-Y715C mutation was generated by site-directed mutagenesis of the pcDNA4/TO-GFP-CLC7 expression vector using the Q5™ Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) and complementary primer pairs (forward ...
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bioRxiv - Biochemistry 2022Quote: Cysteine mutations were introduced into a pENTR1A FLAG (FT) WT F3 or pENTR1A 3xFT WT F3 plasmid using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA). pENTR1A constructs were shuttled into the pcDNA DEST40 vector (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... Quantification of enriched sequencing libraries was performed using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs, Ipswich, Massachussets, USA). Libraries were then pooled and sequenced on an Illumina Miseq System (Illumina ...
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bioRxiv - Physiology 2022Quote: ... in zebrafish f5 proximal promoter sequences (Fig. 4E, Supplemental Table 5) (28) was conducted using a Q5 site-directed mutagenesis kit (NEB, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: In vitro transcription/translation by codon skipping of the short FLAG tag-containing peptides X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag) where X = 7, 13, 14, or 15 was carried out using the PURExpress® Δ (aa, tRNA) Kit (New England Biolabs, E6840S) based on a previous protocol with slight modifications 16 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 500 ng of RNA was used for cDNA library construction with NEBNext ultra II directional RNA library prep kit for Illumina as recommended by the manufacturer (New England Biolabs, Inc, Ipswich, MA). cDNA libraries were validated on the TapeStation (Agilent ...
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bioRxiv - Genetics 2022Quote: ... We inserted TFT reporters into BFA0190 by digesting the plasmid with SwaI and inserting TFT components between the LYP1 flanking sequences using isothermal assembly cloning (Hifi Assembly Cloning Kit; New England Biolabs [NEB], Ipswich, MA, USA). The 5’ and 3’ LYP1 flanking sequences in each TFT plasmid contain natural SacI and BglII restriction sites ...
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bioRxiv - Plant Biology 2022Quote: ... and pCambia1305.1 vectors were prepared and subsequently digested using NcoI restriction endonuclease prior to purification using Monarch DNA & PCR Cleanup Kits (New England Biolabs, Ipswich, MA, USA). Vector and insert concentrations were estimated using a Synergy H1 microplate reader (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: ... Five microliters of resulting clean RNA was used for first- and second-strand cDNA synthesis and library preparation using NEB Ultra II Directional RNA library prep kit for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were barcoded with unique dual indices synthesized in the CDC Biotechnology Core Facility Oligonucleotide Synthesis Laboratory ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted from organoids (three biological replicates for each group) by TRIzol and the rRNAs were removed with NEBNext rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA). RNA libraries were constructed using the NEBNext® Ultra(tm ...