Labshake search
Citations for New England Biolabs :
9451 - 9500 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... polyA-enriched fraction was used for library construction using the NEBnext Ultra II Directional Library Prep Kit for Illumina (New England Biolabs, Cat. No. E7760L), following the standard protocol but using half the reaction volumes recommended ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA (tissue samples) or 100ng RNA (cell culture experiments) using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2023Quote: Both small RNA-seq and mRNA-seq libraries were quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, Cat. No E7630L). Libraries were sequenced on an Illumina NextSeq500.
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA; E11.5 samples) and the TruSeq stranded mRNA Library prep (Illumina ...
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bioRxiv - Genomics 2022Quote: ... and one short read library using the NEBNext Ultra II FS DNA Library Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA). The long-read library was then sequenced on the PacBio Sequel IIe system in CLR mode using the Sequel II Binding Kit 2.2 (PacBio) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 viral RNA detection and quantification was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, Whitby, ON, Canada) on the Rotor-gene Q platform (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... The library preparation of the total RNA was performed with the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420S/L). Single read sequencing with a read length of 72 bp was performed on NextSeq ® 2000 System using the corresponding NextSeq2000 P3 Reagent Kit (Illumina) ...
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bioRxiv - Genetics 2023Quote: ... A strand-specific RNA sequencing library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). Then ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries were generated with about 330ng DNA and the NEBNext Ultra II FS DNA Library Prep Kit in combination with NEBNext Multiplex Oligos (New England Biolabs, Ipswich, MA, USA). We sequenced 2×125bp reads with an Illumina HiSeq 2500 at the VBCF-NGS facility (https://www.viennabiocenter.org/vbcf/next-generation-sequencing/) ...
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bioRxiv - Genomics 2023Quote: ... Samples were then processed following the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Ipswich, MA, Cat#T3050L).
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bioRxiv - Microbiology 2023Quote: ... using the NEBNext Ultra II Directional RNA Library Prep Kit and the NEBNext Multiplex Oligos for Illumina (New England BioLabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 750 uL TRIzolTM LS and libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, version 3.0, #E6420L) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Genomics 2022Quote: ... The successful amplification of a COI gene fragment of approximately 680 bp was confirmed by agarose gel electrophoresis before the amplicon was purified with the DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) and sequenced (Eurofins Genomics ...
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bioRxiv - Cell Biology 2023Quote: ... The ChIP and evDNA were purified and used for constructing sequencing libraries with a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The library quantifications were assessed on the Agilent Bioanalyzer 2100 system ...
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bioRxiv - Immunology 2023Quote: ... Libraries from purified progenitor RNA were generated using the NEBnext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). rRNA depleted RNA was fragmented ...
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bioRxiv - Genomics 2023Quote: ... The resulting cDNA was sent to Biotools Co., Ltd (New Taipei City, Taiwan) for library preparation using the NEBNext® DNA Library Prep Kit (NEB, USA,20015828,20015829), and sequenced for 150bp paired-ends on an Illumina Hiseq 2500 sequencer ...
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bioRxiv - Genomics 2023Quote: ... Libraries of the fragmented DNA were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... Libraries for RNA-Seq were prepared with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England BioLabs, USA) for three replicates per treatment resulting in 24 libraries with a mean insert size of 300 bp ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis of ATG8-Interacting Motifs (AIM) was performed using Q5 site-directed mutagenesis kit (New England BioLabs, Frankfurt am Main, Germany) using primers listed in Supplemental Table S5 according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified for high-throughput sequencing by an Ultra II DNA Library Prep Kit (New England Biolabs, Catalog Number: E7645S). Then ...
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bioRxiv - Genomics 2023Quote: ... The other library was shipped frozen to the University of Oregon (Eugene, OR) and prepared for Illumina Novaseq sequencing using the NEB Ultra II kit (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions with a few modifications by the Genomics and Cell Characterization Facility (GC3F ...
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bioRxiv - Genomics 2023Quote: LIDAR and 3’-LIDAR libraries were analyzed on a 2% agarose gel and quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, Cat. No E7630L). Libraries were sequenced on an Illumina NextSeq500.
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bioRxiv - Genomics 2023Quote: ... a standard whole-genome 150 base pair (bp) paired-end Illumina library was prepared using the NEBNext Ultra II library preparation kit (New England Biolabs Inc., Ipswich, USA). Finally ...
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bioRxiv - Neuroscience 2023Quote: RNA Library preparations for transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA was used for library preparation with NEB Next® Ultra IITM DNA Library Prep Kit for Illumina® (New England Biolabs, USA) and sequenced on Illumina NovaSeq 6000 platform (150 bp ...
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bioRxiv - Physiology 2023Quote: mRNA was extracted from mesenteric and renal artery whole cell lysates using the Monarch Total RNA Miniprep Kit’ (New England Biolabs, Ipswich, Massachusetts, USA). The concentration and purity of the RNA samples were calculated using a NanoDrop 3300 Fluorospectrometer with absorbance set to 260nm and 280nm ...
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Microbiology 2023Quote: ... PCR was conducted using a Rapid Barcoding Kit 96 (ONT, Oxford, UK) with Q5® Hot Start High-Fidelity 2X Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol designed for SARS-CoV-2.
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bioRxiv - Immunology 2023Quote: Library preparation and sequencing were performed by Novogene UK using the NEB Next Ultra RNA Library Prep Kit (New England BioLabs, Cat. No. E7530L). Samples were sequenced using 150-bp paired-end reads using NovaSeq 6000 (Illumina).
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Molecular Biology 2023Quote: ... At day 4 post nucleofection genomic DNA was extracted and 500 ng of gDNA from each sample were randomly digested with NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E6177) to obtain fragments of ca ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA pellet was resuspended in 0.1 X TE and used for DNA library generation with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit) ...
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bioRxiv - Cell Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Genetics 2023Quote: ... RNA libraries for RNA-seq were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760L) following manufacturer’s protocol for use with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was fragmented and TruSeq-Adapters ligated with the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® (NEB) and 3’-end-fragments were finally amplified using primers with Illumina P5 and P7 overhangs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Some libraries have been instead prepared with the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Samples were either double indexed with a set of 60 dual-index primers ...
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bioRxiv - Biochemistry 2023Quote: ... was constructed by replacing the gRNA region in pPvU6b-DmtRNA-BbsI (16) using the NEBuilder HiFi Assembly kit (New England BioLabs, Ipswich, MA, USA) as previously described (16 ...
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bioRxiv - Bioengineering 2023Quote: ... and whole mRNAseq libraries were generated using the NEB Next® UltraTM Nondirectional RNA Library Prep Kit for Illumina® (New England Biolabs, MA), following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760). The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... We then ligated barcodes to the end-prepped DNA using the Native Barcoding Expansion 1–12 kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase Master Mix (New England Biolabs, Ipswich, MA, USA). We cleaned the barcoded samples using 0.9X AMPure XP beads ...
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bioRxiv - Genomics 2023Quote: ... using the Adapter Mix II from the Native Barcoding Expansion 1–12 kit and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA) as well as Quick T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... the transcriptome sequencing library was prepared and sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). All samples were clustered using the TruSeq PE Cluster Kit v3 cBot HS (Illuminia ...
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bioRxiv - Cell Biology 2024Quote: ... a total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations and sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA).
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bioRxiv - Cell Biology 2024Quote: ... to pull down mRNA and used for cDNA library construction using NEBNext ultra II directional RNA library prep kit for Illumina as recommended by the manufacturer (New England Biolabs, Inc, Ipswich, MA). The quality of the cDNA libraries were assessed on the TapeStation (Agilent ...
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bioRxiv - Genomics 2024Quote: ... Amplicons prepared for long-Illumina read sequencing (PE300) underwent end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA). Custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix with a 15 min incubation at 20°C (Supplemental Table 6) ...
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bioRxiv - Microbiology 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end repaired ...
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bioRxiv - Cancer Biology 2024Quote: ... 800ng RNA was used per sample as input for the RNA library preparation using NEBNext® rRNA Depletion Kit v2 (NEB, E7405L, USA) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2024Quote: ... 5 μg of HMW DNA sample was fragmented by sonication to a size of 350 bp and sequencing library was prepared using NEB Next® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs, USA) following manufacturer’s instruction ...