Labshake search
Citations for New England Biolabs :
9501 - 9550 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The purified ChIP DNA and 220 ng of whole cell extract DNA were used to prepare ChIP-seq libraries using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645S) with PCR amplifications carried out for 16 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The resulting double-stranded cDNA fragments were subjected to end repair ...
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bioRxiv - Plant Biology 2024Quote: ... by Gibson assembly using the NEBuilder® HiFi DNA Assembly kit and following the manufacturer’s instructions (New England BioLabs Inc., Ipswich, MA, USA). The resulting Gibson assembly products were transformed into 50 µl of DH5α competent E ...
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bioRxiv - Cell Biology 2024Quote: ... GFP mRNA was produced in-house using the Hiscribe® T7 Quick high yield RNA synthesis kit and 3’-O-me-m7G cap analog (New England Biolabs, MA, USA) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently used to assemble the RNA-seq library using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB; E7760), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was used as the template for IVT with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Biochemistry 2024Quote: 1μl of total cellular RNA was used for sequencing library generation by NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2024Quote: ... DNA segments exceeding 1 kb in length were manually excised from the agarose gel and retrieved using a Monarch® DNA Gel Extraction Kit (NEB Inc., USA). A Qubit 2.0 Fluorometer and the Qubit (ds)DNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... These PCR products were inserted into EcoRI-XhoI-digested linearized pCAGGS using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswich, MA, USA). Previously constructed plasmids expressing G of VSBV-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The two fragment products of rpfF and the linearized pK18mobsacB (Schäfer et al. 1994) were then isothermally assembled using the Gibson cloning kit (NEB; Gibson et al. 2009) following the manufacturer’s instructions in a 5:1 insert to backbone ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... The precipitated DNA was washed once with 70% ethanol and suspended in 30μl 0.1 X TE (1mM Tris-HCl and 0.1mM EDTA) for library preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... high molecular weight (HMW) DNA was extracted from an overnight culture using the Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, Ipswich, MA, USA) as per manufacturer’s protocol for HMW DNA extracted from bacteria ...
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bioRxiv - Microbiology 2024Quote: ... RNA was synthesized from a DNA template (Supplementary Table 3) using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA) and treated with DNase I (NEB ...
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bioRxiv - Genomics 2024Quote: ... 5 ng of the resulting RNA was applied for library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... and the sgRNA was synthesized in vitro as described in the manual (HiScribe™ T7 High Yield RNA Synthesis Kit, NEB, NO. E2040S), the sgRNA and Cas9 protein were mixed and coinjected into the cell cytoplasm at the one-cell stage ...
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bioRxiv - Developmental Biology 2024Quote: ... dilution of the universal adaptor and 13 cycles (or 16 cycles) of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat. No. E7760L), the NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A library was then prepared following the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit procedure (New England Biolabs, Ipswich, MA, USA). A library was directly prepared from 100 ng of total RNA extracted from floral buds using the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was submitted for small RNA sequencing to the UT Southwestern McDermott NGS Core using the NEBNext Small RNA kit (New England Biolabs, cat. no. E7330), with size selection between 15bp and 30bp ...
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bioRxiv - Genetics 2024Quote: To determine the T-DNA insertion site of line ClvRubq7 we extracted genomic DNA from two different plants and constructed sequencing libraries using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E7805) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end repaired ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were constructed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina according to manufacturer’s instructions (NEB - catalogue no. E7645S). Library PCRs were supplemented with 2x SYBR dye (Sigma – catalogue no ...
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bioRxiv - Genetics 2021Quote: ... Bands corresponding to small RNAs were collected and their content was recovered using the Monarch DNA Gel Extraction kit (NEB #T1020 New England Biolab). The sRNA collected were turned into libraries using the NEBNext Multiplex Small RNA Library Prep Set (NEB #E7300S New England Biolab) ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Microbiology 2021Quote: ... The RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration was determined using a Nanodrop and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Molecular Biology 2021Quote: ... following the manufacturer’s instructions and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Evolutionary Biology 2021Quote: Extracted and purified RNA was reverse transcribed into first-strand copy DNA (cDNA) using the ProtoScript® II Reverse Transcriptase Kit (NEW ENGLAND BioLabs®Inc, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was conducted using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific RNASeq libraries were created using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #6420L; New England Biolabs, Inc., Ipswich, MA, USA). The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626 ...
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
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bioRxiv - Genomics 2020Quote: Whole Genome DNA libraries with an average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Genomics 2020Quote: ... Approximately 300 ng of genomic DNA was used to prepare DNA sequencing libraries for each parent following the protocol of NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB Cat No. #E7805S/L). The libraries were sent to Novogene (https://en.novogene.com/ ...
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bioRxiv - Microbiology 2019Quote: ... sequencing libraries were generated from rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: Shotgun libraries for Illumina MiSeq sequencing were prepared with the NEBNext® Ultra II DNA Library Prep Kit for Illumina® in combination with the Index Primer Set 1 (NEB, Frankfurt, Germany) according to manufacturer’s instructions and as described in (57) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The library was then PCR amplified in five separate aliquots (12 cycles, Phusion High Fidelity® PCR kit, New England Biolabs Inc., Frankfurt, Germany). We used Agencourt® AMPure XP beads (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and transcribed after subcloning into the DR274 vector using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). The Cas9 protein was produced in Escherichia coli as previously described (Ménoret et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA samples were used for RNA-Seq library construction using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). We followed the protocol for normal insertion size option.
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bioRxiv - Genomics 2019Quote: ... Two strand-specific RNA-seq libraries with an insert size of 350 bp using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) were prepared and sequenced on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of RNA was used for cDNA library construction using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7760S, E7335). Library size (~350 bp ...
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bioRxiv - Plant Biology 2021Quote: The RNA-seq libraries were constructed with the NEB Next® Ultra™ II RNA Library Prep Kit for Illumina® following manufacturer’s instruction (NEB, Cat#E7775).
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bioRxiv - Plant Biology 2020Quote: ... A total of 12 sRNA libraries were constructed from 5 μg of size-selected RNA using the NEBNext® Small RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) as per the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Libraries were prepared from 1 μg of total RNA using NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) by poly(A ...
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bioRxiv - Genomics 2019Quote: The genomic DNA was extracted from 10 mg frozen brain cortex samples using NEB Monarch genomic DNA® purification kit (NEB, Ipswich, MA, USA). Four microliters of RNase A (100mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were generated from a total amount of 3 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was inserted to the PstI and BglII sites on the pWALIUM20 vector (Perkins 2015) by Gibson-assembly (NEB HiFi DNA Assembly Kit) to generate pWALEXA20 (White-AttB-LexAop for shRNA expression) ...
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bioRxiv - Microbiology 2021Quote: ... D186N were first introduced into the Rluc-CV-B3 reporter viruses with Q5 site-directed mutagenesis kit (New England Biolabs, Bioké, Leiden, The Netherlands)52,53 ...
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bioRxiv - Microbiology 2019Quote: ... Metatranscriptomic libraries were prepared from total RNA using the NEBNext® Ultra II Directional RNA Sequencing Prep Kit (New England BioLabs, Ipswich, MA, USA) in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs ...