Labshake search
Citations for New England Biolabs :
9101 - 9150 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Sonicated DNA was subjected to end-repair and adapter ligation using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB) following the NEB protocol ...
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bioRxiv - Genomics 2023Quote: The RNA-Seq paired end sequencing libraries were prepared from the QC passed RNA samples using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB) as per manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) in combination with the NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Cell Biology 2023Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Synthetic Biology 2023Quote: ... The assembly reaction is conducted in a 25.0 μL assembly reaction mix composed of 1.0 μL NEBridge® Golden Gate Assembly Kit BsaI-HF® v2 (NEB® E1601), 2.5 μL T4 DNA ligase buffer 10X (NEB® B0202) ...
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bioRxiv - Plant Biology 2023Quote: ... Shotgun libraries for genome assembly were prepared using the NEBNext Ultra II DNA library preparation kit (New England Biolabs, Ipswich, MA, USA) and sequenced on Illumina HiSeq2000 (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg RNA of each sample was used to generate cDNA libraries by NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs). The qRT-PCR was performed using TB Green Premix Ex TaqTM (RR820A ...
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bioRxiv - Genomics 2023Quote: The strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... 150 ng of total RNA per sample was used to synthesize cDNA using LunaScript RT SuperMix kit (Cat# E3010L, NewEngland BioLabs, MA, USA) in a 20 µl reaction volume ...
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bioRxiv - Molecular Biology 2023Quote: 150 ng RNA was used to prepare libraries using the NEBNext Ultra II Directional mRNAseq kit with poly(A)+ purification module (NEB E7760, E7490) as described with modifications ...
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bioRxiv - Plant Biology 2023Quote: ... The enriched mRNAs were further taken for the library preparation using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (Catalog: E7775S, New England Biolabs). Sequence data was generated using Illumina HiSeq ...
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bioRxiv - Synthetic Biology 2023Quote: ... in a 20.0 μL reaction mix composed of 2.0 μL NEBridge® Golden Gate Assembly Kit BsaI-HF® v2 (NEB® E1601), 2.0 μL T4 DNA ligase buffer 10X (NEB® B0202 ...
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bioRxiv - Plant Biology 2023Quote: ... Library concentration was further quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England BioLabs® Inc.). Libraries were equimolarly pooled in batches of ∼15 (five libraries per age cohort ...
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bioRxiv - Genetics 2023Quote: ... unique Y-adapters containing UMI sequences and sample-specific P5 indexes were ligated to the DNA using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs). Y-adapter-ligated DNA fragments were amplified by PCR1 using a TdTomato-specific forward primer and a reverse primer binding to the P5 sequence on Y-adapter ...
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bioRxiv - Genomics 2023Quote: ... The DNA library quality was assessed by a qPCR based on the NEBNext Library Quant Kit (New England Biolabs Inc., Ipswich, USA). Sequencing with standard Illumina primers was performed on a NextSeq 500/550 instrument (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA library was prepared using the NEBNext® Ultra II Directional mRNA Library Prep Kit (New England Biolabs, Frankfurt/Main, Germany). The quality of RNA and the library was determined by Nanodrop and Qubit (both Thermofisher) ...
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bioRxiv - Genomics 2023Quote: ... Libraries were constructed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to manufacturer’s recommendations (NEB, cat. no. E7420) using 14 cycles of PCR ...
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bioRxiv - Genetics 2023Quote: ... were assembled in the pCFJ150 vector to create the pCT3.37 plasmid (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs, cat#E5520). The protocol previously described for the generation of mosSCI transgenic lines (Frøkjær-Jensen et al. ...
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bioRxiv - Genomics 2023Quote: ... The PCR2 product pools were subjected to three steps of purification with the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L), Gel DNA Recover Kit (Zymoclean ...
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bioRxiv - Molecular Biology 2023Quote: ... and ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S, NEB) and sequenced on the Illumina HiSeq 2500 platform configured for 50-bp single-end reads.
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bioRxiv - Genomics 2023Quote: ... An additional USER treatment step was performed to the purified DNA by adding 2μl USER (included in all the NEB index primer kits) and incubating for 15 min at 37⍰ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library preparation was performed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA). The quantity of the prepared DNA libraries was determined using a Qubit© 2.0 Fluorometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... 1.2 μg of RNA was used as the starting material for the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA). Poly-A RNA was purified as per instructions and converted to adapter-ligated ...
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bioRxiv - Pathology 2023Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... The YFP-CESA6 construct was used as a template to prepare 18 YFP-CESA6 mutated constructs using a Q5 Site Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) with the specific primers listed in Supplemental Table S4 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared from total RNA using the Ultra II RNA Sample Prep Kit (New England BioLabs, Ipswitch, MA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Genomics 2022Quote: ... and then used for qPCR or converted into sequencing libraries following the NEBNext® Ultra™ II DNA Library Prep Kit (NEB). For ChIP-qPCR ...
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bioRxiv - Genomics 2022Quote: An Illumina sequencing DNA library was prepared from the same DNA sample using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs). An Omni-C library was prepared for a separate visceral sample using the Dovetail™ Omni-C™ Kit with Library Module and Primer Set for Illumina (Dovetail Genomics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The beads were washed and re-suspended in elution buffer and used for library preparation using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). The amplified final libraries were sequenced on the Illumina HiSeq X Ten platform (San Diego ...
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bioRxiv - Genetics 2023Quote: ... RNA-seq libraries were prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). Sequencing was performed using HiSeq X Ten (Illumina) ...
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bioRxiv - Evolutionary Biology 2023Quote: Sequencing libraries were prepared from 0.5 μg total RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 1 µg of the duplex was in vitro transcribed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB # E2050S) following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Plant Biology 2023Quote: ... pCAMBIA0380 was digested by EcoRI and XhoI and used for the cloning of three PCR fragments with Gibson Assembly cloning kit (NEB, Evry, France) according to the supplier’s indications ...
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bioRxiv - Biochemistry 2023Quote: ... ABCB5β with ABCB6 and ABCB5β with ABCB9 coding sequences were fused following the NEBuilder HIFI DNA Assembly kit’s instructions (New England Biolabs, Ipswich, MA, USA). The ABCB5β-ABCB6 ...
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bioRxiv - Microbiology 2023Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (cat # E7630L, New England Biolabs, Ipswich, USA).
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bioRxiv - Genomics 2023Quote: The 40 libraries were constructed with NEBNext® Ultra II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to the instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we purified the products of the ace-1 PCR (see above) using the BS664-250 Preps EZ-10 Spin Column PCR Purification kit (New England BioLabs, Evry France). The purified PCR products were then cloned (TOPO TA Cloning Kit pCR 2.1-TOPO Vector and TOP10F’ invitrogen bacteria) ...
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bioRxiv - Genomics 2023Quote: ... Illumina libraries for all 4 isolates were constructed using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs Inc., USA) as per standard protocol and sequenced using the Illumina MiSeq and NextSeq500 platforms (paired end ...
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bioRxiv - Bioengineering 2023Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA). GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... 3μg RNAs of each biological sample were collected to construct sequencing library using NEB Next Ultra Directional RNA LibraryPrep Kit for Illumina (NEB, Ispawich, USA). The mRNAs were measured by DEGseq software to find differential expressed genes (DEG ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA libraries were prepared from 50 ng (hippocampi) or 10 ng (amygdalae) RNA using the NEBNext® Ultra™ II RNA Library Prep Kits for Illumina (New England Biolabs). Libraries were sequenced (40M bp sequencing depth ...
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bioRxiv - Biochemistry 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, E7490L, E7760L) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP and input libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswitch, MA, USA). Libraries were sequenced on an Illumina NextSeq500 (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... and library preparation was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, catalog # E6420) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and Illumina-compatible libraries prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs E7760S). Libraries were sequenced on the Illumina HiSeq3000 system ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from HCT116 Ki-67-AID in presence or absence of Ki-67 and extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK) according to manufacturer’s protocol ...