Labshake search
Citations for New England Biolabs :
9051 - 9100 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
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bioRxiv - Microbiology 2024Quote: ... primers were designed to clone Ceg10 into pET28b using restriction enzymes NdeI and NotI by HiFi DNA Assemby kit (NEB; Table S4), producing the His6x-TEV-Ceg10 vector ...
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bioRxiv - Cell Biology 2024Quote: ... followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs, US). All steps were performed as recommended in user manual E7760 (Version 1.0_02-2017 ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genetics 2024Quote: ... The B splice form (Spc105RB) was generated using site directed mutagenesis to delete the first intron from the A form (NEB BaseChanger Kit). All mutants were generated using site specific mutagenesis of the A- or B-form Spc105R coding region in the pENTR4 vector ...
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bioRxiv - Genomics 2024Quote: ... A library for short-read sequencing was prepared using an NEBNext Ultra II DNA Library Prep kit (New England Biolabs, Beverly, MA) and sequenced using a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: Sequencing libraries were prepared using a commercial kit (NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, New England Biolabs, Ipswich, USA), and cDNA of three replicates per strain was sequenced as 150 bp (100 bp for Mpyr_CVe13f ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries for regular cfDNA were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog #: E7760, New England Biolabs Inc.), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Genomics 2024Quote: Libraries for metagenomic sequencing of CS2022-0121 and F16-31-UTM were prepared using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) to elucidate the enrichment efficiency of the target capture sequencing ...
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bioRxiv - Microbiology 2024Quote: ... and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB, cat# E5520) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Genomics 2024Quote: ... Double-stranded DNA libraries with 450 bp insert size were prepared using the ultraFS-II kit (New England Biolabs, London, United Kingdom). The QC check for the prepared libraries was done on an Agilent Tapestation (Santa Clara ...
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bioRxiv - Microbiology 2024Quote: ... we used a similar protocol but library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, ref. E7370) for Illumina following manufacturer’s specifications except for end repair ...
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bioRxiv - Biophysics 2023Quote: Purified linear and circular DNA were both in vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S). 240 ng reaction was prepared according to the manufacturer’s recommendation (see details in Supplementary Methods) ...
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bioRxiv - Microbiology 2023Quote: ... Damaged double-stranded DNA in the initial DNA sample was repaired using the PreCR® Repair Mix Kit (New England Biolabs, USA) before library construction ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... This plasmid was used as a template for G2019S and I2020T site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Catalog # E0445S). These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Sequencing libraries were validated using the Agilent Tapestation 4200 (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... Ab aliquot of DNA (100 ng) was used to create libraries using the NEBNext Ultra II kit (New England Biolabs, Ipswich, Massachusetts). Libraries were sequenced at The University of Texas at Austin Genome Sequencing and Analysis Facility on a HiSeq 4000 ...
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bioRxiv - Genetics 2023Quote: ... We ran amplified DNA from wildtype and mutant individuals on an agarose gel and purified (Monarch DNA Extraction and Purification kit, new England Biolabs, MA, USA) and cloned the PCR products into JM109 competent cells using pGEM®-T Easy Vector Systems (Promega Corporation ...
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bioRxiv - Cell Biology 2023Quote: The 2NSP23 nanobody mRNA was prepared by in vitro translation of a DNA molecule of using the HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (New England BioLabs). The obtained mRNA has the following sequence and includes a 5’-CAP and a poly-A tail ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mutated enhancer sequence was synthesized as a gBlocks Gene Fragment (IDT, Coralville IA) and inserted into pattBnucGFPh using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Beverley, MA).
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bioRxiv - Molecular Biology 2023Quote: ... 100 - 400 ng of PCR fragments were used as template DNA to synthesize analytic amounts of DNA deaminases using PURExpress In Vitro Protein Synthesis kit (NEB, Ipswich, MA) following manufacturer’s recommendations.
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bioRxiv - Evolutionary Biology 2022Quote: ... Whole-genome libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) with modifications for subsequent target enrichment ...
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bioRxiv - Neuroscience 2023Quote: ... The RNA sequencing library was generated using NEBNext Ultra II Directional RNALibrary Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MS, USA). The sequencing library was validated on the Agilent TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... The libraries for Illumina sequencing were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... End-repaired DNA was barcoded using the Native Barcoding Expansion kit (ONT, United Kingdom) and Blunt/TA Ligase Master Mix (New England Biolabs, United States). Barcoded samples were multiplexed and ligated to sequencing adaptor molecules in Adapter Mix II using the NEBNext Quick T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA from cell pellets was isolated using the Quick-DNA Midiprep Plus Kit (ZymoResearch, D4075) and genome-integrated sgRNA sequences were amplified by PCR using the Q5 Mastermix (New England Biolabs Next UltraII). i5 and i7 multiplexing barcodes were added in a second round of PCR ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Microbiology 2023Quote: HTS was performed on the three RNA extracts after a step of rRNA depletion with the rRNA depletion kit (NEB, Evry, France), according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit; New England Biolabs, Ipswich, MA, USA). Specifically ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was amplified using an AMV first strand cDNA synthesis kit and was normalized to 50 ng/ul for each sample (New England Biolabs, Catalog # E6550). Sslac2 was amplified using specific detection primers (Sslac2 Det F and R ...
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bioRxiv - Physiology 2022Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then subjected to DNase treatment to remove any residual genomic DNA using Monarch RNA purification kit (NEB cat no. 2040S).
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bioRxiv - Microbiology 2022Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA Magnetic Isolation Module and used to construct RNA-seq libraries with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s protocol with slight modifications ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot of RNA from each Leptospira serovar was processed with the addition of poly(A) tail using Escherichia coli poly(A) Polymerase Kit (New England Biolabs, Massachusetts, USA) following the protocols from the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: cDNA libraries were prepared from 500 ng RNA using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs; Ipswich, MA) with rRNA depletion ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... sequence samples for Illumina sequencers were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were generated using NEB Next® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Cell Biology 2023Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Genomics 2023Quote: ... Libraries were constructed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to manufacturer’s recommendations (NEB, cat. no. E7420) using 14 cycles of PCR ...
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bioRxiv - Genetics 2023Quote: ... were assembled in the pCFJ150 vector to create the pCT3.37 plasmid (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs, cat#E5520). The protocol previously described for the generation of mosSCI transgenic lines (Frøkjær-Jensen et al. ...
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bioRxiv - Genomics 2023Quote: ... The DNA library quality was assessed by a qPCR based on the NEBNext Library Quant Kit (New England Biolabs Inc., Ipswich, USA). Sequencing with standard Illumina primers was performed on a NextSeq 500/550 instrument (Illumina ...