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Citations for New England Biolabs :
7501 - 7550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and excess primers were removed with an Exonuclease I digestion (New England Biolabs). Whole transcriptome amplification (WTA ...
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bioRxiv - Immunology 2023Quote: ... samples were amplified with NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs) (Buenrostro et al ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3 μl NEBNext Ultra II End Prep Enzyme Mix (NEB, E7546), incubated for 10 min at room temperature followed by 10 min at 65 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75 ...
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bioRxiv - Cancer Biology 2023Quote: ... The end-repaired DNA was ligated with 5 μl Adapter Mix (ONT, SQK-LSK110) using 8 μl NEBNext Quick T4 DNA ligase (NEB, E6056) at 21°C for up to 1h ...
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bioRxiv - Cancer Biology 2023Quote: ... the samples were end-repaired by adding 2 μl NEBNext FFPE DNA Repair Mix (NEB, M6630) and 3 μl NEBNext Ultra II End Prep Enzyme Mix (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... and Amylose Resin (New England Biolabs, cat. E9021V) were used for purifying proteins fused with polyhistidine (His) ...
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bioRxiv - Genomics 2023Quote: ... and 15 units of Phi29 polymerase (New England Biolabs). We used a PCR machine (SimpliAmp ...
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bioRxiv - Genomics 2023Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB8_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Molecular Biology 2023Quote: ... These immuno-enriched DNA samples could be used for NGS after DNA library construction with NEBNext® UltraTM II DNA Library Prep Kit (NEB, USA, Catalog #: E7645L). The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co. ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Plant Biology 2023Quote: ... In vitro transcription was performed using the HiScribe T7 ARCA mRNA (with tailing) kit (New England Biolab, http://www.NEB.com) and Xenopus oocytes were injected with 30 ng of RiSKC3 cRNA using a pneumatic injector ...
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bioRxiv - Plant Biology 2023Quote: ... Three cDNA samples derived from three independent RNA extractions were synthesized and amplified using Luna®Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Biochemistry 2023Quote: ... plates that contain unc-104(R551H) allele was selected by genomic PCR and BamHI (NEB) digestion ...
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bioRxiv - Biochemistry 2023Quote: SipA constructs were cloned into mammalian expression vectors using NEBuilder assembly (New England Biolabs). SipA full-length (a.a ...
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bioRxiv - Biochemistry 2023Quote: ... modified to include a tobacco etch virus (TEV) protease recognition sequence downstream of the 6xHis-tag47 using NEBuilder HiFi DNA assembly master mix (New England BioLabs). Sequences were verified by Sanger DNA sequencing [Genomics Shared Resource ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB9_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Genomics 2023Quote: ... 1× Phi29 reaction buffer (New England Biolabs), and 15 units of Phi29 polymerase (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) (New England Biolabs C2527H) was transformed with pRSET B-GA-MatryoshCaMP6s ...
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bioRxiv - Genomics 2023Quote: ... 1 mM dNTPs (New England Biolabs), 3.5 μM of Phi29 Random Hexamer Primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phusion DNA polymerase and T5 exonuclease were purchased from New England BioLabs (NEB). Sybr Safe DNA gel stain was purchased from Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext ® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DH5α (NEB). Escherichia coli strains and plasmids used are listed in Supporting Information ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was treated with DNaseI and purified using Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The cDNA was synthesised from approximately 1000 ng of RNA using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... Quick CIP and T4 DNA ligase (New England Biolabs) described by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... reactions were quenched with the addition of final concentrations of 15% Proteinase K (New England Biolabs) (∼1.2 U ...
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bioRxiv - Microbiology 2023Quote: ... Cloning was performed using restriction enzymes (New England Biolabs & Vivantis Technologies), Quick CIP and T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The template was gel-purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Massachusettes, USA) and transformed into UKMCC1015 electrocompetent cells ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol and cDNA library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). DNA library quality control was done by measuring concentration using the Qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Taq DNA polymerases (New England Biolabs) and 2xTaq PCR MasterMix (SolarBio ...
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bioRxiv - Microbiology 2023Quote: ... the entire region of pET15b-His-sdeA except for the region encoding 1-199 aa of SdeA was amplified with primers 2649/2712 and then the fragment was self-ligated with a Gibson assembly kit (New England Biolabs). For construction of pmGFP-sdeAΔDUB ...
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bioRxiv - Molecular Biology 2023Quote: ... and m7Gppp5′N cap was added using Vaccinia Capping System (NEB). To induce superovulation ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Poly(A) polymerase (NEB), and m7Gppp5′N cap was added using Vaccinia Capping System (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... and DNase I (NEB) were added to lysate and was then incubated at room temperature on a Nutator mixer ...
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bioRxiv - Bioengineering 2023Quote: ... RNase I (NEB) and DNase I (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Taq DNA polymerase (New England Biolabs) was used for error-prone PCR (EP-PCR) ...
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bioRxiv - Microbiology 2023Quote: ... Subtraction of both bacterial and mammalian rRNA was done using the NEBNext rRNA Depletion Kits (NEB), and sequenced on a Novaseq 6000 instrument (Novogene) ...
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bioRxiv - Biochemistry 2023Quote: ... each protein construct was transformed in either BL21 DE3 pLysS (NEB) harbouring a truncated OGT version with an intact catalytic core (OGTSW ...
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bioRxiv - Molecular Biology 2023Quote: All plasmids were constructed with NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and T4 DNA ligase were from NEB Canada (Whitby ...
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bioRxiv - Physiology 2023Quote: ... The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and amplified using NEBNext® High-Fidelity 2x PCR Master Mix (NEB, Cat. #M0541s) for 9 cycles ...
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bioRxiv - Immunology 2023Quote: ... and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB, Cat.#E7103S). Briefly ...
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Biophysics 2023Quote: Escherichia coli BL21 (DE3) cells (#C2527, NEB) were transformed with the appropriate plasmid and grown at 37°C to an OD600 of 0.8 - 1.0 in MOPS-buffered Terrific broth (TB) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 2µg of RNA was reverse transcribed into cDNA using reverse transcriptase (NEB), and quantitative real-time PCR was performed using SYBR green (Applied Biosystems) ...