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Citations for New England Biolabs :
5851 - 5900 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coli in vitro transcription were synthesized through a 200 µL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 µM dNTP Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Templates for in vitro transcription were generated by PCR amplification (Q5 High-Fidelity DNA Polymerase, New England Biolabs) from plasmids using a T7 extended primer (5’-CCTAATACGACTCACTATAGGGAG-3’ 85) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were ligated to pUCm-T vector (B522211, Sangon) with Blunt/TA Ligase Master Mix (M0367S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplification at the α-tubulin locus was done using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with a 30 s extension time ...
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bioRxiv - Genetics 2023Quote: ... the primary probes were amplified using Phusion® High-Fidelity PCR Master Mix with HF Buffer (M0531S, NEB) by monitoring the amplification on a qPCR machine (CFX Connect ...
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bioRxiv - Cell Biology 2023Quote: Polymerase chain reaction (PCR) was performed using the Phusion Hot Start Flex 2X Master Mix (New England Biolabs). Reactions were set up according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The DNA library was then prepared for Illumina NGS sequencing by PCR using Phusion HF master mix (NEB) and custom indexed primers for the PTM library 56 ...
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bioRxiv - Genetics 2024Quote: Double-stranded RNA was in vitro transcribed from a PCR amplicon using T7 RNA Polymerase (New England BioLabs) (Extended Data Fig ...
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bioRxiv - Biophysics 2024Quote: ... Bisulfite-converted libraries were PCR-amplified and uniquely dual-indexed using NEBNext Multiplex Oligos for Illumina (NEB #E6440S) and the Kapa HiFi Uracil+ PCR system (Kapa #KK2801) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplification and barcoding performed as described (Hart paper, Cell, 2015) using NEBNext Q5 Ultra mastermix (NEB, # MO544). PCR products were extracted from agarose using a Monarch DNA Gel Extraction Kit (NEB ...
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bioRxiv - Genomics 2024Quote: ... Psoralen pull-down DNA was further purified using a Monarch PCR & DNA clean-up kit (New England Biolabs), eluted in 15 μl of EB buffer (10 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic region containing the CRISPR-edit was PCR-amplified using OneTaq polymerases in Standard Buffer (New England Biolabs). After PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... and the adaptor ligated DNA was amplified using NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB, M0543). The libraries were sequenced at a rate of 5 million reads per sample ...
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bioRxiv - Cell Biology 2024Quote: ... The ATAC-seq libraries were constructed and amplified using NEBNext High-Fidelity 2x PCR Master Mix (NEB, #M0541) and SYBR Green I (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: sgRNAs were synthesized (Bhattacharya, Marfo, Li, Lane, & Khokha, 2015) using PCR with Q5 High-Fidelity DNA Polymerase (NEB), 1 µM each of forward and reverse primer and the following cycling conditions ...
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bioRxiv - Microbiology 2024Quote: ... Each strain was streaked for isolation and PCR was performed with each strain using Taq DNA polymerase (NEB) and the primers and reaction conditions listed in Table S7A to amplify one 610-bp region of the eps locus (NE91_04885) ...
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bioRxiv - Microbiology 2024Quote: ... and amplified by PCR using Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs, USA; #M0494) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was carried out on 200-300 ng of restricted genomic DNA using OneTaq 2X Master Mix (NEB) with primers complementary to sequences within the neo cassette that flanked the intron [Neotet1s ...
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bioRxiv - Bioengineering 2024Quote: ... The target genomic locus was amplified by PCR using Q5 Hot Start high-fidelity 2X master mix (NEB) and primers listed in Table S3 and purified by PureLink PCR purification kit (Thermo Fisher Scientific) ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... The digested pWallium20 backbone and the amplified PCR fragment were assembled using a HiFi assembly reaction (NEB #E5520), with an incubation time of 1 hour at 50 °C ...
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bioRxiv - Genetics 2024Quote: We amplified the oligo pool using five cycles of PCR with Phusion High-Fidelity polymerase (New England Biolabs) and primer set KC_1 (Table S4) ...
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bioRxiv - Plant Biology 2024Quote: ... PCR on both cDNA and gDNA were amplified using OneTaq Hot Start 2X Master Mix (New England Biolabs). cDNA reactions were done using 10% total volume of the reaction as input and 100 ng of gDNA as input ...
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bioRxiv - Synthetic Biology 2024Quote: All PCR amplifications for plasmid construction and cloning were performed using Q5® High-Fidelity DNA Polymerase (NEB), followed by purification with Monarch® PCR & DNA CleanupKit (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... extracted PCR product with 2 μl of NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery PCR reactions were carried out with 1 µl of the selection product in Taq DNA polymerase (NEB) reactions with recovery primers (CSR_Rec_SHORT_F1/R1 ...
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bioRxiv - Genomics 2024Quote: ... 2 μL of 10% Tween-20 and 5 μL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
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bioRxiv - Synthetic Biology 2021Quote: ... The polymerase chain reaction (PCR) products were amplified using Q5 High-Fidelity DNA polymerase (New England Biolabs, MA, USA) with strict accordance to the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: The purified DNA was PCR amplified for 15 cycles using Q5 High Fidelity DNA polymerase (New England Biolabs, M0491S) with the incorporation of Illumina Nextera XT adaptors (Illumina ...
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bioRxiv - Genetics 2021Quote: ... 20 μl of this product was used for PCR amplification using Q5 Hot Start High-Fidelity polymerase (NEB #M0494S) and a unique combination of the dual barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293 ...
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bioRxiv - Developmental Biology 2021Quote: ... Ascl2 promoter regions are amplified by Nested PCR with Touchdown protocol with LongAmp DNA Taq polymerase (New England Biolabs). The PCR for both rounds were performed as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR addition of indices (7-11 cycles) was done using NEBNext Ultra II DNA library prep kit (NEB). Biotinylated capture probes 70 nt in length were designed against every DpnII restriction fragment in a 2.5 Mb window centered on Runx1 (chr16:91,566,000-94,101,999) ...
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bioRxiv - Genetics 2021Quote: ... A 25μl PCR mix was prepared using 12.5μl OneTaq® Quick-Load® 2X Master Mix (New England Biolabs) with Standard Buffer ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions for cloning were performed by using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Inc (NEB). All the products sequenced to verify the absence of any mutations (GATC-biotech).
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bioRxiv - Molecular Biology 2021Quote: ... nts 3619 to 5070) were amplified from genomic DNA by touchdown PCR with Q5 DNA polymerase (New England Biolabs) using a forward primer that introduces the T7 promoter sequence for in vitro transcription (IVT) ...
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bioRxiv - Developmental Biology 2021Quote: ... The eluted DNA was then amplified in a reaction with 25μl NEBNext High-Fidelity 2x PCR Mastermix (NEB; M0541L) and custom 25um Nextera PCR Primers (Ad1_noMx universal primer ...
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bioRxiv - Developmental Biology 2021Quote: ... and duck were amplified by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (M0493L, NEB, Ipswich, MA, USA). pGL3 was digested with HindIII-HF (R3104S ...
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bioRxiv - Systems Biology 2022Quote: ... we performed a standard PCR using all 10μl of eluted DNA with 25μl 2x NEB High-Fidelity master mix (NEB, MO544), 2.5μl of 25μM forward primer (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng of gel or PCR purified DNA was used as a template for in vitro transcription (NEB E2040S) carried out according to the manufacturer’s instructions with the addition of 0.1μl of Cy3 (Sigma PA53026 ...
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bioRxiv - Molecular Biology 2021Quote: ... eight rounds of linear amplification PCR reactions with 40μg genomic DNA were performed with 5 μg genomic DNA each using HS Flex polymerase (NEB) and biotinylated Myc primer (Table S2) ...
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bioRxiv - Immunology 2022Quote: ... PCR fragments were assembled into a linearized pcDNA vector using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... PCR products were cleaned using one unit each of Exonuclease I and Antarctic Phosphatase (New England Biolabs, Massachusetts, USA), with sequencing reactions performed using an ABI3730 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: The 254bp V4 segment of the 16S ribosomal RNA subunit was amplified by a 17-cycle touchdown PCR using the following protocol: 12.5 μl Phusion 2X Master Mix (New England BioLabs),1 μl each of 16S V4 forward and reverse primers (primer pair 515F-806R) ...
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bioRxiv - Genomics 2020Quote: ... were added to each sample in a 9-cycle PCR reaction prepared by adding 12.5 μl Phusion 2X Master Mix (NEB) and 2 μl each of Nextera XT S5 and N7 adapters ...
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bioRxiv - Biochemistry 2020Quote: ... encompassing a T7 promoter followed by hammerhead ribozyme and the specific 5’ tRNA isoacceptor or scrambled RNA sequences were PCR-amplified and afterwards in vitro-transcribed using 1.875 U/µL T7 Polymerase (NEB) in 1x T7-Buffer with 20 mM DTT ...
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bioRxiv - Developmental Biology 2019Quote: ... and the Tol2-5xUAS-TATA:M2H37A Bleeding Heart plasmid was assembled from the digested pBH and 5xUAS-TATA:M2H37A PCR product using Gibson Assembly Master Mix (NEB). The entire plasmid was sequenced to confirm ligation fidelity.
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were generated using 18 cycles of PCR amplification with NEB high fidelity 2x master mix (New England Biolabs), cleaned up with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2019Quote: ... libraries were generated using the NEBNext Ultra II library preparation kit with 15 cycles of PCR amplification (NEB E7645). The libraries for the ‘MicroChIP’ samples were generated using the MicroPlex library prep kit (Diagenode C05010012 ...
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bioRxiv - Genetics 2019Quote: ... The constructed library of each sample was amplified by PCR with Phusion high-fidelity DNA polymerase (New England Biolabs). Finally ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR was performed on 0.5 – 1 ng of genomic DNA with the LongAmp Taq DNA Polymerase (New England Biolabs) using standard PCR reaction mix and long extension times (9 minutes at 65°C).
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bioRxiv - Cancer Biology 2019Quote: ... The second round PCRs were performed with NEBNext® Ultra(tm) II Q5® Master Mix (NEB, cat. M0544). Amplified samples were purified with AMPure XP SPRI beads (Beckman Coulter ...