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Citations for Bioline :
151 - 200 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Gene expression levels were assessed using SensiFAST SYBR reaction kit (Bioline Reagents Ltd.) for quantitative real-time PCR (qRT-PCR) ...
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bioRxiv - Immunology 2024Quote: ... DNA was extracted using ISOLATE II Fecal DNA Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cDNA synthesis was performed using the SensiFAST cDNA Synthesis kit (Bioline) and 200 ng of RNA as template ...
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bioRxiv - Immunology 2024Quote: ... the aqueous phase was mixed 1:1 v/v with 70% ethanol and loaded into an Isolate II RNA Mini kit column (Bioline, Cincinnati, OH, USA). Total RNA was purified according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using the SensiFAST SYBR Hi-ROX kit (Bioline) and predesigned KiCqStart SYBR green primers for the genes of interest (MilliporeSigma ...
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bioRxiv - Microbiology 2024Quote: Tissue DNA was isolated using the ISOLATE II Genomic DNA kit (Bioline) according to the manufacturer’s instructions using Proteinase K ...
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bioRxiv - Developmental Biology 2024Quote: ... and qPCR was performed using gene-specific primer sets (listed in Supplemental Table 1) and SensiFast SYBR Hi-ROX master mix (Bioline). MiRNA qRT-PCR was performed with the polyA and SYBR Green method as previously described using miRNA-specific forward primers and a 3’ RACE adaptor reverse primer62 ...
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bioRxiv - Microbiology 2024Quote: ... in conjunction with HyperLadder 100 bp (Bioline) to determine the presence of the target sequences ...
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bioRxiv - Microbiology 2024Quote: ... and then cDNA was synthesized using SensiFAST cDNA synthesis kit (Bioline) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... SensiFAST cDNA synthesis kit (Bioline), propidium iodide (PCR biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... coli (alpha-select competent cells, Bioline) and plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of 10 mM dNTP mix (Bioline). This mixture was incubated at 50 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: PCR amplification conditions included a 20 μl PCR mix including 1X MyTaq mix (Bioline, London, UK), 0.5 μM of each primer ...
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bioRxiv - Immunology 2024Quote: ... 3 µL of 5x PyroTaq EvaGreen qPCR Mix Plus with ROX (Cultek Molecular Bioline, #88H24), and transcript-specific forward and reverse primers at a 20 μM final concentration ...
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bioRxiv - Microbiology 2024Quote: ... 5’-[FAM]-CACAGGAGC-[ZEN]-CAAGAGTGAAGAACAGT-[3IABkFQ]-3’) was used for quantification using the SensiFAST™Probe Lo-ROX One-Step Kit (Bioline). 0.2 μL of 10 μM probes and 0.8 μL of 20 μM primers were used for both targets ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR (qPCR) was performed using a SensiFAST™ SYBR® No-ROX Kit (Roche Bioline, cat. no. BIO-98020). Expression levels of all genes were normalized to the geometric mean of housekeeping genes ...
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bioRxiv - Immunology 2024Quote: ... Tetro cDNA synthesis kit (Bioline) was used to prepare the cDNA ...
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bioRxiv - Microbiology 2024Quote: ... falciparum infection by rapid diagnostic test (RDT; SD BIOLINE Malaria Ag P.f test of histidine-rich protein II ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA samples were then used as templates for real-time PCR assays performed on a Bio-Rad CFX system using the SensiFAST SYBR No-ROX Kit (BioLine). Alpha-tubulin mRNA was used as a reference gene for normalization ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA samples were then used as templates for real-time PCR assays performed on a Bio-Rad CFX system using the SensiFAST SYBR No-ROX Kit (BioLine). Beta-actin mRNA was used as reference gene for normalization ...
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bioRxiv - Plant Biology 2024Quote: ... 0.15 unit Taq polymerase (Bioline; Meridian) and 1.0 ng of template DNA ...
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bioRxiv - Plant Biology 2024Quote: ... Each 6 µl of reaction mixture contained 1xNH4 reaction buffer (Bioline; Meridian), 0.2 mM dNTP ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with Trisure reagent (BIO-38032, Bioline), and RNA was isolated using the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from frozen tissues using TRIsure (Bioline) as previously described [19] ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 μL 10 μM specific primer pairs (mixture of forward and reverse primers) was mixed with 10 μL SensiFAST SYBR (Bioline) mastermix and 9.6 μL of cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was extracted from the cells using TRIsure (Bioline), treated with DNase I (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... SensiFAST SYBR Hi-ROX kit (Bioline) was used in the qPCR-reactions ...
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bioRxiv - Immunology 2024Quote: BC1 tissue pieces from replicate wells were collected in 1 mL of TRIsure (BioLine, cat. BIO-38032) and homogenized in gentleMACS M tubes (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... cells were fixed with 4% PFA or treated with Trisure reagent (BIO-38032, Bioline) for qRT-PCR analysis or lysed with the Lysis buffer for Western blot analysis or coprecipitation experiments.
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bioRxiv - Neuroscience 2024Quote: The total RNA was extracted from myotubes or Tibialis muscles using Trisure Reagent (BIO-38032, Bioline) following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2024Quote: ... where we used MyTaq DNA Polymerase (Bioline, Heidelberg, Germany). The SNPs were selected after a search in the GTEx portal (https://www.gtexportal.org).
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bioRxiv - Pathology 2024Quote: ... or with a Isolate II RNA Mini Kit (Bioline) as per the manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... 40 mM dNTPs (Bioline, London, UK), 200 U/μL M-MLV-RT (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... and the concentration was increased using Co-precipitant pink (Bioline), if necessary ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR reaction was performed using the SensiFASTTM SYBR No-ROX Kit (Bioline) on the Roche LightCycler 480 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... and reverse-transcribed with the SensiFASTTM cDNA Synthesis Kit (Bioline) to generate cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Each combination of DNA sample and primer pair was amplified using 5ul BioMix Red (Bioline), 0.5ul forward primer ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 μM final concentration of sense and antisense primer (see Table S1 for sequences) and MyTaq Red Mix (Bioline) was used ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were run on 1% agarose gel and the corresponding band was gel extracted using PCR gel extraction kit (Bioline) and further sequenced by Sanger method using one of the PCR primers ...
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bioRxiv - Microbiology 2024Quote: ... Standard plasmid isolation was carried out (Bioline) followed by PCR to detect the transposition and mini-circle formation and DNA sequencing to confirm the exact location were performed as described above.
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bioRxiv - Microbiology 2024Quote: ... The PCR products were run on 1% agarose gel and the corresponding band was gel extracted using PCR gel extraction kit (Bioline) and further sequenced by Sanger method using one of the PCR primers ...
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bioRxiv - Microbiology 2024Quote: ... Standard plasmid isolation was carried out (Bioline). The resulting plasmid DNA templates were diluted to 10 ng/µL and 10 ng was used as template for PCR to detect transposition ...
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bioRxiv - Microbiology 2024Quote: ... Standard plasmid isolation was carried out (Bioline) followed by PCR to detect the transposition using primers that are complementary to the cargo gene (cat ...
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR (qPCR) was performed in duplicate using SYBR Green containing supermix (Bioline) on a Bio-rad C1000 Touch Thermal Cycler (Bio-rad ...
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesised from 1 µg of RNA using the sensiFAST cDNA synthesis kit (Bioline, UK). 2 µl of cDNA (corresponding to 20 ng ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR reactions were setup using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2µM ...
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bioRxiv - Genetics 2024Quote: ... The polymerase chain reactions (PCRs) were carried out using BIOLASE DNA polymerase (Bioline) with 35 cycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used the primer pairs desatF_for/desatF_rev (Tm = 60) and eloF_for/eloF_rev (Tm = 62) with MyTaq Red (Bioline, Meridian Bioscience) to distinguish between D ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed using BIOTAQ (BioLine, London, UK) with the primers listed in Supplementary Table 2 ...