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Citations for Bioline :
301 - 350 of 1617 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Total RNA was prepared using Trizol (BIOLINE, BIO-38032). Tetro cDNA synthesis kit (BIOLINE ...
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bioRxiv - Microbiology 2023Quote: ... Increasing amounts of poly(dT) (average length 221bp) (Bioline) or additional unlabeled ATP (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 1 kb Hyperladder (Bioline). PCR products were purified using Mag-Bind RXNPurePlus beads (OMEGA Biotek ...
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bioRxiv - Developmental Biology 2023Quote: ... For real-time PCR SYBR Green Master mix (Bioline BIO-94020) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... expanded and initially screened for correct integration using homology- arm spanning PCRs (Immolase DNA polymerase, Bioline, supplemented with Q solution, Qiagen). Genotypes of positive clones were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using SensiFAST™ SYBR® Lo-ROX Kit (Bioline) and either the QuantStudio™ 5 or QuantStudio™ Flex 7 analyser (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was generated using the Tetro cDNA Synthesis Kit (Bioline) and the Uni12 primer (5’-AGCAAAAGCAGG-3’ ...
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bioRxiv - Genomics 2023Quote: ... Bisulfite PCR reactions used MyTaq HS DNA polymerase (Bioline), and contained 1× reaction buffer ...
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bioRxiv - Physiology 2023Quote: ... 800 nM of forward and reverse primers and SensiFAST SYBR Lo-ROX (Bioline). We ran gene-specific qPCR plates and each sample was analysed in duplicate ...
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bioRxiv - Genomics 2023Quote: ... PCR reactions were prepared using the MyTaq DNA polymerase kit (Bioline, Cat# BIO-21111). Reactions contained a 5× MyTaq Buffer ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cultures (ODMCs and VSMCs) using RNA isolation kit (Bioline) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 ng of the total RNA was reverse-transcribed into cDNA using a Tetro cDNA synthesis kit (BIO-65042, Bioline, London, UK). Quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... and SYBR green chemistry (Bioline, London, UK), were conducted to confirm and validate template cDNA quality ...
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bioRxiv - Molecular Biology 2023Quote: ... Routine PCR genotyping of was performed using MyTaq Red mix (Bioline) and using the mentioned primers in 2:1:1 combination ...
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bioRxiv - Molecular Biology 2023Quote: Cells were harvested in TRIsure (Bioline #38033). RNA was extracted using Direct-zol RNA MicroPrep columns (Zymo #R2062 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primers were re-evaluated for specificity and to ensure that they produced only a single band of the appropriate length using MyTaq DNA Polymerase (Bioline, Alexandria, NSW, Australia) and agarose gel electrophoresis.
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bioRxiv - Molecular Biology 2023Quote: ... Tetro cDNA synthesis kit (BIOLINE, Cat# BIO-65043) was employed to synthesis cDNA ...
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bioRxiv - Plant Biology 2023Quote: ... A total of 1 µg of total RNA was used to initiate cDNA synthesis using the SensiFAST™ cDNA Synthesis Kit (Bioline, London, UK). The SYBR Green assay with SensiFAST™ SYBR no ROX kit was used for the quantification of transcripts in RT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was performed with the Bio-Rad CFX96 cycler using the SensiFAST™ SYBR® (Bioline). Gene expression for occludin (Ocln) ...
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bioRxiv - Neuroscience 2023Quote: ... Ligated plasmids were transformed into α-select chemically competent cells (BIO-85026; Bioline, London, UK). Positively transformed cells were selected using antibiotic Ampicillin LB agar plates and colonies were picked to grow in Ampicillin LB medium at 37°C for overnight ...
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bioRxiv - Neuroscience 2023Quote: ... or 1 μg (4 weeks of treatment) of the total RNA was reverse-transcribed into cDNA using a Tetro cDNA synthesis kit (BIO-65042, Bioline). Quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated using TRIsure (Bioline, Cat. No. BIO-38032) following standard phenol-chloroform RNA extraction ...
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed using the SensiFast cDNA Synthesis Kit (Bioline). Quantitative real-time PCR was performed in triplets for each sample using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Genotyping PCR on genomic DNA was performed using the MyTaq DNA Polymerase system (Bioline, cat. no. BIO-21105). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant bands were compared to a 1 Kb HyperLadder (Bioline) using the expected sizes per gene region as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared for RT-qPCR using the SensiFAST cDNA preparation kit according to manufacturer instructions (Bioline #65054). 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1µL of cDNA was used per RT-qPCR reaction prepared with SYBR Lo-ROX (Bioline #94020). cFos primers were from9 ...
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bioRxiv - Plant Biology 2023Quote: ... and the 200 to 400 bp range was purified by gel extraction using Isolate II PCR and Gel Kit (Bioline). Libraries were sequenced (100 bp paired-end ...
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bioRxiv - Plant Biology 2023Quote: ... and further purified with Isolate II Gel and PCR Clean-up Kit (Bioline). Libraries were then generated from fragmented DNA (Covaris ...
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bioRxiv - Microbiology 2023Quote: ... If a band was visualised at ∼480 bp the PCR product was purified using an ISOLATE II PCR and Gel Kit (Bioline).
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the ISOLATE II Genomic DNA Kit (Bioline). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... PCR products that produced bands of the correct size were purified using the ISOLATE II PCR and Gel Kit (Bioline) as per manufacturer’s protocol and submitted for sequencing using an Applied Biosystems 3730xl capillary analyser (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from the FTATM card using an ISOLATE II Genomic DNA Kit (Bioline) with a pre-lysis in 180 µL of Lysis Buffer GL and 25 µL Proteinase K for 2 hours before completing the extraction as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Standard qPCR was performed with SensiFast syb Green Mix (Bioline #B2092020) in ABI qPCR plate (GeneFlow #P3-0292 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR reactions were performed with different sets of primers and Sensifast SYBR (Bioline, Taunton, MA) on a CFX96 or CFX384 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... Trisure (Bioline) was used for RNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... Indexing PCRs were created by combining 10 μl of purified DNA with 10 μl 2x MyTaq HS Mix polymerase (Bioline, Narellan, NSW, Australia) and 1 μl (5 μM ...
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bioRxiv - Microbiology 2023Quote: ... 7.5 μL of 2x MyTaq HS Mix polymerase (Bioline), 0.45 μL of 10 μM forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... both samples were subjected to PCR with human GAPDH-specific primers41 and MyTaq polymerase (Bioline, Taunton, MA). Resulting PCR amplicons were visualized by agarose gel electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... were recruited if they had a capillary haemoglobin of < 10 g/dL (i.e., moderate or severe anaemia) and a negative malaria rapid diagnostic test (mRDT, SD Bioline Malaria AG Pf/Pan ...
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bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
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bioRxiv - Microbiology 2023Quote: ... Indexing PCRs were created by combining 10 μL of purified DNA with 10 μL 2x MyTaq HS Mix polymerase (Bioline) and 1 μl (5 μM ...
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bioRxiv - Microbiology 2023Quote: ... was performed in technical triplicate with 50 ng of cDNA per reaction using the SensiFAST SYBR & Fluorescein Kit from Bioline, on a Roche Lightcycler 96 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was isolated using the Isolate II Genomic DNA kit (Bioline). Library preparation and whole genome sequencing was performed at the Hartwig Medical Foundation using the HiSeqX system generating 2 × 150 base read pairs ...
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bioRxiv - Microbiology 2023Quote: ... or Isolate II RNA mini kit (Bioline). DENV-2 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR assays were carried out in the 7500 Real Time PCR System from Applied Biosystems using SensiFAST SYBR Lo-ROX Kit (BioLine, London, UK) and primers previously described [7 ...