Labshake search
Citations for Bioline :
51 - 100 of 1617 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... and SensiMix™ SYBR® reagents (Bioline). The reactions were run on a LightCycler® 96 (Roche Diagnostics) ...
-
bioRxiv - Molecular Biology 2024Quote: ... After selection on LB-agar kanamycin plates the resulting colonies were first screened by colony PCR using MyTaq DNA polymerase (Bioline) to check for the presence of the right size insert using T7forward and T7reverese primers ...
-
bioRxiv - Cell Biology 2024Quote: RNA from cell pellets treated with dTAG-13 for four hours was isolated using the Isolate II RNA Mini Kit (Bioline BIO-52072), and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414) ...
-
bioRxiv - Biophysics 2024Quote: ... Total RNA was extracted from both cells and tissue powder by TRIsure reagent (Bioline, Ecogen, BIO-38033) following the manufacturer’s protocol and using phenol-chloroform ...
-
bioRxiv - Pathology 2024Quote: ... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
-
bioRxiv - Cancer Biology 2024Quote: ... PCR products were separated by gel electrophoresis on 2% DNA grade agarose (Bioline) gels in TAE buffer (40 mM Tris Acetate ...
-
bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using the SensiFast Sybr No-Rox Mix (Bioline) on a RealPlex 2 Thermocycler (Eppendorf).
-
bioRxiv - Genetics 2024Quote: RT-qPCR was performed (in triplicate) using the Bio-Rad cycler CFX 96 and the SensiFAST SYBR Green Kit (Catalog Number BIO-98005, BIOLINE, USA). Gene expression levels were normalized to housekeeping genes pmp-3 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Single organoids were hand-picked with capillary pipet tips and examined for locus deletion by PCR using MyTaq™ Red Mix (Bioline, #BIO-25043) and the following cycling parameters ...
-
bioRxiv - Cancer Biology 2024Quote: ... and quantitative RT-PCR analyses were performed using SensiFAST™ SYBR® kit (Bioline) on an Applied Biosystems StepOne Plus instrument ...
-
bioRxiv - Developmental Biology 2024Quote: ... using the Sensifast SYBR no Rox kit (Bioline, BIO-98020). Data analysis and visualisation was performed using Microsoft Excel (Office 365 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 0.625 units of Immolase™ Taq polymerase (Bioline, London, UK), 2 µL genomic DNA and PCR-grade H2O ...
-
bioRxiv - Genomics 2024Quote: ... in the presence of RiboSafe RNase Inhibitor (Bioline). Reverse transcription was conducted using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher ...
-
bioRxiv - Genomics 2024Quote: ... RT-qPCR was conducted using SensiMix SYBR Hi-ROX 2x Master Mix (Bioline) on the Rotor-Gene Q (Qiagen) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... dNTPs (0.2 mM each) (Bioline, London UK), MgCl2 (1.5 mM ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... MgCl2 (1.5 mM) (Bioline, London UK), 0.5 µM each of forward and reverse primers ...
-
bioRxiv - Developmental Biology 2024Quote: Snap frozen cell pellets had total RNA extracted using ISOLATE II RNA mini kit (Bioline)
-
bioRxiv - Genomics 2024Quote: ... using 10 µL SensiFAST SYBR No-ROX mix (2x) (Bioline Inc., Taunton, MA), 300 nM forward and reverse primer ...
-
bioRxiv - Cell Biology 2024Quote: ... Real time qPCR was performed using a RotorGene 6000 (Corbett Research) instrument with SYBR (Bioline, UK) and results were analysed using beta-actin (Actb ...
-
bioRxiv - Microbiology 2024Quote: ... Single colonies were tested (n = 24-48) for the loss of the vector (erythromycin susceptibility) and by discriminatory PCR (MyTaq HS - Bioline) with specific oligonucleotides to select mutant over WT genotypes ...
-
bioRxiv - Microbiology 2024Quote: ... or Accuzyme (Bioline). All plasmids are listed in Table S4.
-
bioRxiv - Microbiology 2024Quote: ... The qPCR was performed as described (24) using 2x SensiFast mastermix (Bioline) with primers and probes to a final concentration in 25uL reaction volume of 0.32uM (primers ...
-
Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA was used for reverse transcription with a Sensifast cDNA synthesis kit (Bioline). RT-qPCR was performed with LightCycler 480 SYBR Green I Master reagent with the primers listed below.
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized utilizing a gene-specific primer designed to target the 10xSunTag socRNA and Tetro Reverse Transcriptase (Bioline). The resulting cDNA was isolated via column-based purification using the GeneJet Gel Extraction Kit (Thermo Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... genomic DNA was isolated using the ISOLATE II Genomic DNA Kit (Bioline) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: DNA was extracted from lung tissue and digested using proteinase K (Bioline: BIO-37084) for genomic excision gels ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated from cells 3 hours after inducing socRNA expression using TRIsure (Bioline). Subsequently ...
-
bioRxiv - Immunology 2024Quote: Total RNA was isolated from mouse tissues using TRIsure reagent (BIO-38033, Bioline Gmbh, Germany) and genomic DNA was digested using RNase-free DNase (rDNase ...
-
bioRxiv - Immunology 2024Quote: ... Quantitative PCR was performed using 2X Taq based Master Mix (Bioline) and Taq Man gene expression assays (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 1 µg of RNA using the SensiFAST cDNA synthesis kit (Bioline, #BIO65054). cDNA was then diluted (1:5 ...
-
bioRxiv - Genomics 2024Quote: ... cDNA was synthesized using SensiFASTTM cDNA Synthesis Kit (BioLine; BIO-65053) and following all protocol specifications from the vendor ...
-
bioRxiv - Molecular Biology 2023Quote: ... individuals testing HIV negative by rapid immunoassays (SD Bioline HIV1/2 Elisa 3.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The final product was diluted to 1:20 and 2 µL of the dilution were used in each 10-µl RT-qPCR reaction along with 400 nM of primers gene- or isoform-specific primers (Table S4B) and 1X SensiFAST SYBR No-ROX Mix (Bioline, cat#BIO-98005). Quantitative PCR was performed in CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was performed using 35 μL SensiFAST™ Probe LoROX One-Step Kit (Bioline, Taunton, MA) and 15 μL of extracted eluate ...
-
bioRxiv - Plant Biology 2023Quote: ... the extracts were reverse transcribed using random hexamers and Tetro RT enzyme (Bioline). The obtained cDNA was amplified with the MangoTaq™ DNA Polymerase and the primers described by Gaafar et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... and MyTaq Master mix (Bioline), to perform PCR ...
-
bioRxiv - Plant Biology 2023Quote: ... and MyTaq Master mix (Bioline) to perform PCR ...
-
bioRxiv - Microbiology 2023Quote: ... and SensiFAST cDNA Synthesis Kit (Bioline, UK), according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions and first strand cDNA synthesis was performed with oligo dT primers using BioScript reverse transcriptase (Bioline). GdMYBSG6 sequences have been deposited in GenBank under the following accession numbers ...
-
bioRxiv - Plant Biology 2023Quote: ... The total RNA was extracted from ground root samples using an ISOLATE II Plant Mini Kit (Bioline, Australia), cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline ...
-
bioRxiv - Plant Biology 2023Quote: ... and real time PCR reactions were completed in duplicate for each sample using a SensiMix SYBR (Bioline); 95◻ for 10 min ...
-
bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed using SYBR green supermix (Bioline) as per the manufacturer’s instructions on a CFX384 Touch real-time PCR detection system ...
-
bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline, Australia) and real time PCR reactions were completed in duplicate for each sample using a SensiMix SYBR (Bioline) ...
-
FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... RT-qPCR standards were generated from Col-0 cDNA pool using MyTaq polymerase mix (Bioline) and purified using Favorprep PCR Purification Kit (Favorgen ...
-
bioRxiv - Cell Biology 2023Quote: ... were used for complementary DNA synthesis and SensiFAST SYBR kit reagents (Bioline) were used for Q-PCR ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.25uM forward and reverse oligo primers (see supplementary table 6) and SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 384-well plates with the LightCycler 480 Instrument II (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... which were subsequently separated using 1% agarose gels prepared with molecular grade agarose (Bioline, BIO-41025), Tris base-acetic acid-EDTA (TAE ...
-
bioRxiv - Neuroscience 2023Quote: ... The Ranger (Bioline, #BIO21121) or Q5 (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: PCR for cloning purposes was performed using the proofreading Bio-X-ACT (Bioline) or Phusion (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... and HyperlLadder 1 kb (Bioline) was used for size determination.