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Citations for Bioline :
51 - 100 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was isolated using the ISOLATE II Genomic DNA Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from lung tissue and digested using proteinase K (Bioline: BIO-37084) for genomic excision gels ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated from cells 3 hours after inducing socRNA expression using TRIsure (Bioline). Subsequently ...
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from mouse tissues using TRIsure reagent (BIO-38033, Bioline Gmbh, Germany) and genomic DNA was digested using RNase-free DNase (rDNase ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR was performed using 2X Taq based Master Mix (Bioline) and Taq Man gene expression assays (Applied Biosystems) ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized using SensiFASTTM cDNA Synthesis Kit (BioLine; BIO-65053) and following all protocol specifications from the vendor ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gel contained 1.95 g of agarose powder (Bioline, final concentration 1.3% w/v) in 150 mL of 0.5 x TBE buffer (50 mM Tris ...
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bioRxiv - Evolutionary Biology 2024Quote: ... thermocycler using qPCR MasterMix No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline, Madrid, Spain). The PCR program consisted of an initial step of pre-incubation denaturation at 95°C for 15 min followed by 45 cycles of denaturation at 95°C for 15 s ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... We synthesized complementary DNA (cDNA) from 100 ng of RNA in a 40µl reaction containing 100 units of Tetro Reverse transcriptase (Bioline, Taunton, MA), 8µl of 5X RT buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... and 0.06 units of Velocity DNA Polymerase (Bioline, UK) and 50 ng of template DNA ...
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bioRxiv - Genetics 2024Quote: ... and 0.12 units of Velocity DNA Polymerase (Bioline, UK) and 0.5 μL of first round PCR product as template.
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bioRxiv - Plant Biology 2024Quote: ... cDNAs were diluted to 50 µL and used as template for Real-Time PCR monitored by SensiFast SYBR No-Rox Kit (Bioline, Cincinatti, OH, USA). RT-PCR was performed in the Roche Light Cycler 480 using primers for CAH4 ...
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bioRxiv - Plant Biology 2024Quote: ... This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline), a LightCycler® 480 (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Gels were prepared by dissolving 1.5g molecular grade agarose (Bioline Reagents Ltd., London, UK) in 150ml 0.5x Tris-borate EDTA (TBE ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using the MasterMix qPCR No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline, Madrid, Spain). Two microliters of the diluted DNA sample were mixed with 0.3 μL (10 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using Random hexamers and Tetro Reverse Transcriptase (Bioline). Quantitative PCRs (qPCRs ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from siRNA-treated cells was isolated using TRIsure (Bioline). Next ...
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bioRxiv - Microbiology 2024Quote: ... 1 ng/μl of total DNA was amplified using the primer sets in SensiFAST SYBR No-ROX (Bioline). qPCR was realized in technical duplicates in the Rotor-Gene Q real-time PCR cycler (Qiagen) ...
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bioRxiv - Physiology 2024Quote: ... 10 μl SensiFAST SYBR no-ROX Mix (Bioline Cat.# BIO-98005). Cycling conditions were ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from leaves using TRIsure™ (Bioline, USA) and treated with DNase I (NZYtech ...
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bioRxiv - Genomics 2024Quote: ... BS-treated DNA was PCR-amplified using the IMMOLASE DNA polymerase Kit (Bioline). Primers used for the PCR were designed with PyroMark Assay Design 2.0 software (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... PCR was conducted using BioTaq DNA polymerase (Bioline). qPCR was conducted using PowerUp SYBR Green (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... These library pools were further qPCR-based quantified using the JetSeq Library Quantification Lo-ROX kit (BIO-68029, Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... The measurement was done with a Sensifast Sybr no-ROX kit (Bioline) in a Bio-Rad CFX96 Real-time PCR detection system ...
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bioRxiv - Genetics 2024Quote: ... Reaction volume of 10 μl contains 2× SensiFAST SYBR No-ROX mix (Bioline), 0.4 μl of each 10 μM forward and reverse primers ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from ∼ 50 mg of mouse gastrocnemius (androgen deprivation experiment) or spinalis (DHT treatment experiment) by phenol chloroform extraction (1 mL TRIsure solution; Bioline), purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: Isolation of total RNA was performed by using the Isolate II RNA Mini Kit (Bioline) followed by cDNA synthesis (SensiFAST cDNA Synthesis Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by cDNA synthesis (SensiFAST cDNA Synthesis Kit, Bioline). Quantitative real time PCR was performed with PowerUp SYBR Master mix in a QuantStudio 6 Pro system ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time RT-PCR was performed on 1:10 diluted cDNA using the SensiFAST SYBR No-ROX Kit (Bioline). Three biological and technical replicates were analyzed per genotype ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCRs were conducted using the SensiFAST SYBR No-ROX kit (Bioline) using primers previously described (Stanton-Turcotte et al. ...
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bioRxiv - Microbiology 2024Quote: ... and precipitated as per the Co-Precipitant Pink (Bioline) protocol before resuspension in 4 μL molecular-grade water (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... HTLV-2 and microbial contaminants and by us for the absence of mycoplasma using EZ-PCR Mycoplasma Test kit (Biological Industries; #20-700-20) and the HyperLadderTM 1kb (Bioline/Meridian Bioscience; # BIO-33026).
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with SensiFast cDNA synthesis kit (Bioline) using the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... cDNA synthesis was performed using a SensiFAST cDNA synthesis kit (BIOLINE) with equalized amounts of RNA between samples ...
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bioRxiv - Genomics 2024Quote: ... Pre-processed samples were homogenized in TRISure (Bioline Meridian Bioscience), and total RNA was isolated using Direct-zol-96 MagBead RNA kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2024Quote: ... were used at a concentration of 500 nM in 2× PCR premix reagent (My Taq Red PCR Mix, Catalog #: MER-BIO-25044, Bioline).
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bioRxiv - Cancer Biology 2024Quote: Quantitative RT-PCR analyses were performed in technical triplicates with SensiMix SYBR kit (Bioline, Cat# QT605–20) using the ViiA™ 7 Real Time PCR System (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1 μL of MangoTaqTM DNA polymerase (Bioline, Australia) and autoclaved Milli-Q water to final volume ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μL of purified genomic DNA (50 ng μL-1) were used directly in 10 μL reaction volumes containing 5 μL of SensiFAST HRM mix (Bioline), 1.6 μL sterile distilled water and 0.7 μL of each primer (100 nM) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted using the Bioline Isolate II RNA extraction Kit (Bioline, BIO-52072) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Specific genomic loci were amplified using Velocity DNA Polymerase (Bioline). Off-target loci represent the top predicted off-target sites in the CRISPR Design Tool (crispr.mit.edu)68 ...
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bioRxiv - Microbiology 2024Quote: ... 0·5 U BioTaq (all Bioline, Meridian Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were generated using BioMix™ Red (Bioline, Bio-25006) and were subsequently incubated with the respective restriction enzyme (BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... 1μg of total RNA was used to synthesize cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and genotyping PCRs were performed with MyTaq HS Red Mix (Bioline) using the following FBXO22 genotyping primers ...
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bioRxiv - Physiology 2024Quote: ... cDNA synthesis was performed using the SensiFAST ™ cDNA Synthesis Kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... 800 nM of forward and reverse primers and SensiFAST SYBR Lo-ROX (Bioline). We ran gene-specific qPCR plates and each sample was analysed in duplicate ...
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bioRxiv - Systems Biology 2024Quote: ... 14 PCR cycles were performed using MyTaq Red Mix (#BIO-25043; Bioline), yielding 30 µg of barcodes ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were collected 24 hours after transfection in 5 ml of TRIsure (#BIO-38032; Bioline) and frozen at −80 °C until further processing.